##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512926_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2338462 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.524200949170865 31.0 30.0 33.0 16.0 34.0 2 28.65857987001713 31.0 30.0 34.0 16.0 34.0 3 28.875550682457103 31.0 30.0 34.0 18.0 34.0 4 32.28611583168767 35.0 33.0 37.0 22.0 37.0 5 32.42932748105379 35.0 33.0 37.0 23.0 37.0 6 32.42727014593353 36.0 33.0 37.0 23.0 37.0 7 32.256780738793275 36.0 33.0 37.0 22.0 37.0 8 32.20236206532328 35.0 33.0 37.0 21.0 37.0 9 33.505465985763294 38.0 33.0 39.0 19.0 39.0 10 33.395132356223876 38.0 33.0 39.0 18.0 39.0 11 33.43188471739118 38.0 33.0 39.0 18.0 39.0 12 33.331838191084564 38.0 33.0 39.0 17.0 39.0 13 33.32910306004545 38.0 33.0 39.0 17.0 39.0 14 34.27581034030059 39.0 33.0 41.0 17.0 41.0 15 34.20453956489351 39.0 33.0 41.0 17.0 41.0 16 34.0971848163451 39.0 33.0 41.0 17.0 41.0 17 34.021333252368436 39.0 33.0 41.0 16.0 41.0 18 33.7420137680236 39.0 33.0 41.0 10.0 41.0 19 33.95674892301008 39.0 33.0 41.0 11.0 41.0 20 34.02892200087066 39.0 34.0 41.0 11.0 41.0 21 33.94681119470832 39.0 34.0 41.0 10.0 41.0 22 33.850971279413564 39.0 34.0 41.0 8.0 41.0 23 33.58091044455715 39.0 33.0 41.0 2.0 41.0 24 33.569386631042114 39.0 33.0 41.0 2.0 41.0 25 33.46725069725315 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8485680334052219 1101 2 1.0461809593655538 1101 3 1.1183665663023383 1101 4 1.0474879538885986 1101 5 0.8715294715668307 1101 6 1.0897006299002996 1101 7 1.1477488319516311 1101 8 0.8760509949261674 1101 9 0.8421626286839441 1101 10 0.9900362028939043 1101 11 1.0693973058548707 1101 12 1.2953658819144493 1101 13 1.1197470597770547 1101 14 1.1795181991352592 1101 15 0.9066046966110051 1101 16 1.3605557282724448 1101 17 1.2631337879120466 1101 18 1.4854865560067552 1101 19 1.0768934116523496 1101 20 1.2524312902431376 1101 21 1.3351891014835786 1101 22 1.422979148397161 1101 23 1.5970530550498339 1101 24 1.2443434571196406 1101 25 1.5876434860469288 1102 1 0.4027300748695559 1102 2 0.5252279191953058 1102 3 0.556036043810753 1102 4 0.7039193341343974 1102 5 0.5106271174019383 1102 6 0.43394104452033133 1102 7 0.6407240329076984 1102 8 0.5686075107248527 1102 9 0.532785950652773 1102 10 0.7109266208335967 1102 11 0.7115955601782957 1102 12 0.7581056547330505 1102 13 0.6659281520275684 1102 14 0.7178346031603198 1102 15 0.9258723528477191 1102 16 0.8789019597565897 1102 17 0.7976744398005806 1102 18 1.1182254464939945 1102 19 1.0609154973089616 1102 20 1.020324198512121 1102 21 0.8161148010296557 1102 22 1.1058748638193805 1102 23 1.3461550717739428 1102 24 1.0950082923420794 1102 25 0.9556510828325671 1103 1 0.5223612714276662 1103 2 0.6804913217116706 1103 3 0.7053796586566072 1103 4 0.8439990648970763 1103 5 0.37036387216354427 1103 6 0.7636881344899606 1103 7 0.5789939103394914 1103 8 0.7104104579306565 1103 9 0.9208677222205566 1103 10 0.8803461692017862 1103 11 0.9166923820489288 1103 12 1.0118860740911373 1103 13 1.0348299102824399 1103 14 1.2307562227312872 1103 15 1.2725320438014478 1103 16 1.4518116544130493 1103 17 1.0935881465263932 1103 18 1.4126937475068218 1103 19 1.5345493618092618 1103 20 1.3808165222892086 1103 21 1.2540582737752146 1103 22 1.3860729748192568 1103 23 1.7476588607624848 1103 24 1.5800600438473964 1103 25 1.5191236760529137 1104 1 0.7250484335942708 1104 2 0.7600919256224259 1104 3 0.8955240159407118 1104 4 1.115279131518136 1104 5 0.9396065026429596 1104 6 0.8336387746233243 1104 7 1.133455798513566 1104 8 1.063543339427092 1104 9 1.2601128673621886 1104 10 1.3605079751910552 1104 11 1.3508883457431935 1104 12 1.1610736226928964 1104 13 1.2443607459605275 1104 14 1.3544074255080787 1104 15 1.502255463143129 1104 16 1.3997575168281386 1104 17 1.4762439600769923 1104 18 1.7307922906399966 1104 19 1.5456958084879986 1104 20 1.5737130143424167 1104 21 1.6158699584603653 1104 22 1.5367510590132838 1104 23 1.8594912910610688 1104 24 1.7385805996367267 1104 25 1.3358991878048627 1105 1 0.8089401602530195 1105 2 0.9367043392161776 1105 3 0.9806094229856512 1105 4 1.0518233161065424 1105 5 0.7440207948108863 1105 6 0.5608006564389427 1105 7 0.8287599339484615 1105 8 1.000396073617253 1105 9 0.8415780031716977 1105 10 1.096771861402523 1105 11 0.9958202967019076 1105 12 1.0458407196094228 1105 13 0.9674333012740632 1105 14 1.1423338768068447 1105 15 1.3351145894339709 1105 16 1.3365384046308861 1105 17 1.2529260340343598 1105 18 1.3004606951955893 1105 19 1.3124739414830415 1105 20 1.3831701988732448 1105 21 1.3690770607542362 1105 22 1.172810050745312 1105 23 1.3317437219508719 1105 24 1.5049978298484916 1105 25 1.6147740733780012 1106 1 0.6960877712359022 1106 2 0.7388691297434953 1106 3 0.7810395698363948 1106 4 0.6768346927734186 1106 5 0.6745230951262826 1106 6 0.7069867312328313 1106 7 0.5200860314106848 1106 8 0.8005751563479606 1106 9 0.8272520066741578 1106 10 1.0572758057628988 1106 11 0.8329288175610685 1106 12 0.8843583922636924 1106 13 0.7396345945756266 1106 14 1.0664144503525677 1106 15 1.1308526775195418 1106 16 1.0933329439966428 1106 17 1.0553771330255586 1106 18 1.2372835502818376 1106 19 0.830752798463358 1106 20 0.949514877360869 1106 21 0.9038123311581714 1106 22 0.903951268818858 1106 23 1.1537206020759427 1106 24 1.1336692279646599 1106 25 1.241051136628542 1107 1 0.4125306502063246 1107 2 0.3815207879564788 1107 3 0.5391050590617823 1107 4 0.6139205238950964 1107 5 0.5317125764422101 1107 6 0.457403306698005 1107 7 0.6572086657229406 1107 8 0.6246154781486908 1107 9 0.7630127307511927 1107 10 0.6472727507274456 1107 11 0.5572990708366206 1107 12 0.65215359630416 1107 13 0.7000362284396147 1107 14 0.9085277332088353 1107 15 0.9783402420289349 1107 16 0.9134496970052908 1107 17 0.9418126589079421 1107 18 1.2249707484203398 1107 19 1.1368915802612776 1107 20 0.9519328922824286 1107 21 0.9511329154325026 1107 22 0.9564447536177099 1107 23 1.018570459540797 1107 24 1.0813044376498482 1107 25 1.12405707462635 1108 1 0.23490727821160462 1108 2 0.29779321804545944 1108 3 0.17177069880058582 1108 4 0.3997679676222887 1108 5 0.35874310202378723 1108 6 0.49012528846242986 1108 7 0.4916575353346886 1108 8 0.5302611572860911 1108 9 0.5538246947170578 1108 10 0.5527095793231211 1108 11 0.4657016451542475 1108 12 0.5655608962904779 1108 13 0.6398629178653223 1108 14 0.6933618096169738 1108 15 0.6458407915371609 1108 16 0.6730251031736785 1108 17 0.7608342764356593 1108 18 0.8204374535282071 1108 19 0.7888908615977215 1108 20 0.6633475676181391 1108 21 0.711587126583936 1108 22 0.6388425374345417 1108 23 0.7450318980094437 1108 24 0.8154239560799539 1108 25 0.9065154251002809 1109 1 0.11710004420043418 1109 2 0.1626780918879973 1109 3 0.3590812335437512 1109 4 0.39738229764855504 1109 5 0.27943635351297047 1109 6 0.2927070301851771 1109 7 0.3589312916101619 1109 8 0.43353408055692455 1109 9 0.5735322240510001 1109 10 0.6919251303073679 1109 11 0.20233558012066055 1109 12 0.43652004163107705 1109 13 0.5368971056941803 1109 14 0.37292417210975515 1109 15 0.6344543695686298 1109 16 0.5658603274815448 1109 17 0.45196093336625154 1109 18 -1.1611530601635707 1109 19 0.3669537598728283 1109 20 0.45168701114198484 1109 21 0.5794752307637054 1109 22 0.6815194992414746 1109 23 0.1350155060871856 1109 24 0.5483769016536328 1109 25 0.6362652695055218 1110 1 0.1077758636933055 1110 2 0.16373524134699124 1110 3 0.2743742079377256 1110 4 0.061721708191633695 1110 5 0.28723781152608296 1110 6 0.1260126985245762 1110 7 0.2601657172258527 1110 8 0.08346778295673118 1110 9 0.36237858396559375 1110 10 0.6036366006776674 1110 11 0.41717887772422557 1110 12 -0.04106049090858477 1110 13 0.1848084291050327 1110 14 0.19017231431522674 1110 15 0.369696653073305 1110 16 0.522542639276196 1110 17 -0.01504613687856704 1110 18 0.44122367436830245 1110 19 0.3626617738289113 1110 20 0.20367439309374902 1110 21 0.3807701351950925 1110 22 0.3625434310307014 1110 23 0.29071043761668136 1110 24 0.5851494411510885 1110 25 0.6825318446083202 1111 1 0.17543430022782047 1111 2 0.49817001670395555 1111 3 0.4194671657107989 1111 4 0.3893550790526703 1111 5 0.3943860915850266 1111 6 0.5552863525820939 1111 7 0.474349772908468 1111 8 0.20283105505775723 1111 9 0.6180995918479866 1111 10 0.7124732340099769 1111 11 0.5166886573492775 1111 12 0.5985445319817444 1111 13 0.6205270924894037 1111 14 0.5274228475311773 1111 15 0.7553650041595219 1111 16 0.5200801296090702 1111 17 0.546013481817937 1111 18 0.7582422910987319 1111 19 0.45715999224643866 1111 20 0.4411264115255946 1111 21 0.6734846469297793 1111 22 0.27560661210540616 1111 23 0.7169150021497757 1111 24 0.8705189080767539 1111 25 0.13335285162528976 1112 1 -0.35589714190298594 1112 2 0.015875363419361577 1112 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-2.0798598998391533 2215 17 -2.4411313381137667 2215 18 -1.251557971160544 2215 19 -1.9829594118073892 2215 20 -2.125577401079859 2215 21 -1.7686665952214682 2215 22 -1.3084994015088043 2215 23 -1.920150379177901 2215 24 -1.6741873481049119 2215 25 -1.6241530894872085 2216 1 -2.6141049442947484 2216 2 -2.7008883498238454 2216 3 -3.156589607826522 2216 4 -3.865737427985003 2216 5 -3.0528995179829117 2216 6 -3.061435121493954 2216 7 -2.8613756254327107 2216 8 -3.4542481333293225 2216 9 -3.7865525593769718 2216 10 -3.455015518510269 2216 11 -3.9623800910784936 2216 12 -4.549993004847753 2216 13 -4.927768415791284 2216 14 -4.388613839902327 2216 15 -4.2406011866936595 2216 16 -3.466025886137551 2216 17 -3.500583285045593 2216 18 -3.5502271807645904 2216 19 -3.981495713545243 2216 20 -3.6921514316448025 2216 21 -4.280445368504502 2216 22 -4.806626046637067 2216 23 -4.649182106444556 2216 24 -4.43744492156177 2216 25 -4.725532912781038 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 131007.0 3 4.0 4 1082.0 5 6291.0 6 7511.0 7 6944.0 8 5939.0 9 5679.0 10 5298.0 11 5011.0 12 4796.0 13 4812.0 14 4800.0 15 4874.0 16 5098.0 17 5476.0 18 6084.0 19 7361.0 20 9176.0 21 12176.0 22 14685.0 23 17968.0 24 21028.0 25 23881.0 26 26353.0 27 29254.0 28 33266.0 29 39590.0 30 47550.0 31 59270.0 32 74120.0 33 93398.0 34 119519.0 35 154343.0 36 207994.0 37 317148.0 38 819676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.086235902013634 18.587883885808456 12.77294731601806 25.552932896159852 2 15.008911237571112 19.725065005403213 41.187714385210164 24.078309371815507 3 18.680858454583255 24.063011743688815 29.434598621832002 27.821531179895924 4 12.285765375441047 16.452988162339196 38.02570473266872 33.23554172955103 5 13.32789442994403 37.64004926671062 35.9199069425591 13.11214936078625 6 32.467978506774266 36.86020635241756 17.81805179468777 12.853763346120402 7 28.40605856084159 31.252408527287944 22.145448744186922 18.19608416768354 8 25.126310152376323 36.7555271919709 20.554164941468738 17.563997714184033 9 26.322450673019755 15.060843381223881 20.087212410766604 38.529493534989754 10 14.816463906771082 28.655249152369112 34.827439160269066 21.700847780590742 11 34.44958558632542 22.13736344109201 24.794504025231802 18.618546947350772 12 23.206199531818665 24.508989158603807 31.749845914191848 20.534965395385683 13 28.909436505981223 21.811783143083957 25.746283977991297 23.532496372943527 14 20.88906017879073 21.860821441541404 27.513445235448287 29.736673144219587 15 22.958254180976827 30.87371796708944 23.109422527505664 23.05860532442806 16 22.495783730052647 27.95355965246729 27.38571588050644 22.16494073697362 17 21.84844331979996 28.53150915085349 27.64418347217446 21.97586405717209 18 21.84907685683641 27.498957831330458 29.2521123710343 21.399852940798826 19 23.560128355494417 26.995331672000557 27.27644081266719 22.168099159837837 20 23.737089017131378 27.21631766877736 27.661155243180723 21.38543807091053 21 23.117937144731883 26.75085238683284 26.89675586126894 23.234454607166338 22 23.284691857239828 27.88103119301999 26.9875718084458 21.846705141294382 23 22.513777180096533 27.63468786838238 27.38904258649982 22.462492365021276 24 23.233561875269995 26.695565887228884 27.93204477311902 22.138827464382103 25 22.83659840533943 27.247685663410365 27.62340312593165 22.292312805318556 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 172.0 1 172.0 2 338.0 3 504.0 4 504.0 5 504.0 6 925.5 7 1347.0 8 1347.0 9 1347.0 10 2202.5 11 3058.0 12 3058.0 13 3058.0 14 5740.5 15 8423.0 16 8423.0 17 8423.0 18 16218.5 19 24014.0 20 24014.0 21 24014.0 22 39363.0 23 54712.0 24 54712.0 25 54712.0 26 79746.0 27 104780.0 28 104780.0 29 104780.0 30 136182.5 31 167585.0 32 167585.0 33 167585.0 34 193805.5 35 220026.0 36 220026.0 37 220026.0 38 240877.5 39 261729.0 40 261729.0 41 261729.0 42 275985.5 43 290242.0 44 290242.0 45 290242.0 46 297429.0 47 304616.0 48 304616.0 49 304616.0 50 295787.5 51 286959.0 52 286959.0 53 286959.0 54 258209.5 55 229460.0 56 229460.0 57 229460.0 58 199041.0 59 168622.0 60 168622.0 61 168622.0 62 137889.5 63 107157.0 64 107157.0 65 107157.0 66 83597.5 67 60038.0 68 60038.0 69 60038.0 70 43946.5 71 27855.0 72 27855.0 73 27855.0 74 19475.5 75 11096.0 76 11096.0 77 11096.0 78 7637.0 79 4178.0 80 4178.0 81 4178.0 82 2749.5 83 1321.0 84 1321.0 85 1321.0 86 867.0 87 413.0 88 413.0 89 413.0 90 265.5 91 118.0 92 118.0 93 118.0 94 69.0 95 20.0 96 20.0 97 20.0 98 18.5 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3567301927506199 2 0.041223676074274455 3 0.03703288742771958 4 0.035835519242989625 5 0.04195064961500337 6 0.018088812219313376 7 0.017746707023676245 8 0.02420394259132712 9 0.021039469531683643 10 0.020312495990954738 11 0.019671048749135115 12 0.020483548588773305 13 0.020440785439318664 14 0.027582231398243805 15 0.028694073284064484 16 0.02116775898004757 17 0.023006574406597157 18 0.08501314111582742 19 0.024802626683692104 20 0.022664469210960023 21 0.026299336914604555 22 0.02014144339313617 23 0.023006574406597157 24 0.01932894355349798 25 0.02287828495823323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2338462.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.73222214782095 #Duplication Level Percentage of deduplicated Percentage of total 1 82.23933275503784 58.992100864729416 2 11.9220396746675 17.103887967967683 3 3.1572451410675155 6.7942862950255 4 1.128104555472459 3.2368578631647686 5 0.5314435067655428 1.9060811843161447 6 0.28200344955555134 1.2137240453982376 7 0.17087554206025157 0.8580097639886701 8 0.1104649035186836 0.6339114398991855 9 0.07687629000848198 0.4963056401509863 >10 0.35002981855015686 4.312876983673078 >50 0.01748799825311935 0.850120784833325 >100 0.011770645641215377 1.6754325203715041 >500 0.0014908457702537852 0.7012379839641474 >1k 8.348736313384833E-4 1.2251666625173494 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3401 0.14543747129523593 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3168 0.13547365747230444 No Hit CTGTAGGACGTGGAATATGGCAAGA 3107 0.1328651053555713 No Hit CTTTAGGACGTGAAATATGGCGAGG 2775 0.11866773973663032 No Hit GTATCAACGCAGAGTACTTTTTTTT 2737 0.11704274005735393 No Hit GTCCTACAGTGTGCATTTCTCATTT 2576 0.11015787299515664 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.276314945464156E-5 7 0.0 0.0 0.0 0.0 4.276314945464156E-5 8 0.0 0.0 0.0 0.0 4.276314945464156E-5 9 0.0 0.0 0.0 0.0 4.276314945464156E-5 10 0.0 0.0 0.0 0.0 8.552629890928312E-5 11 0.0 0.0 0.0 0.0 8.552629890928312E-5 12 0.0 0.0 0.0 0.0 2.1381574727320776E-4 13 0.0 0.0 0.0 0.0 2.1381574727320776E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 955 0.0 12.672355 1 TAGGACC 715 0.0 11.82347 4 TAGTACC 90 7.4769378E-6 11.609437 4 GACGTGA 945 0.0 10.952108 7 TACGACC 70 0.0014936848 10.855577 4 CGTGCGC 80 3.7869535E-4 10.682988 10 GGACGTG 1700 0.0 10.668111 6 AGGACGT 1720 0.0 10.657663 5 TTAGGAC 1140 0.0 10.581808 3 CTTATAC 235 0.0 10.509124 3 GTCCTAA 985 0.0 10.448272 1 GGCGAGG 830 0.0 10.296415 19 AGGACCT 1355 0.0 10.23403 5 TGTAGGA 1990 0.0 10.214608 2 GTCCTAG 300 0.0 10.164493 1 GTCCTAC 1960 0.0 10.161251 1 TACACCG 75 0.0026530053 10.131223 5 GTATAAT 340 0.0 10.089754 1 GTCCTAT 255 0.0 10.089753 1 GGACCTG 1300 0.0 10.079495 6 >>END_MODULE