FastQCFastQC Report
Thu 26 May 2016
SRR1512926_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512926_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338462
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32640.13957891981995005No Hit
GTATCAACGCAGAGTACTTTTTTTT31860.13624339416248799No Hit
CTGTAGGACGTGGAATATGGCAAGA31700.13555918377121373No Hit
CTTTAGGACGTGAAATATGGCGAGG30130.12884536930683502No Hit
GTCCTAAAGTGTGTATTTCTCATTT29200.12486839640755334No Hit
GTCCTACAGTGTGCATTTCTCATTT24500.1047697161638718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7500.013.4477134
CTTCGCA500.001497014313.3034489
CCGATCA655.44153E-513.1572569
GGTATCA8050.012.6349311
GGCGAGG8450.011.4547319
ACCGATC600.005859289311.0878698
GACGTGA10300.010.9809257
AGGACGT18000.010.6778215
GGACGTG17550.010.6226736
TCCAACG909.6287E-510.54361618
GTAGGAC18400.010.540533
CCTACAC3550.010.44446853
AGGACCT14300.010.3798815
TTAGGAC13200.010.371433
TGTAGGA19000.010.1571982
GTCCTAC20700.010.1026971
GGACCTG13700.09.99762256
GTATCAA16750.09.9880991
TAGGACG19450.09.9796294
ACGTGAA12100.09.9751548
Produced by FastQC (version 0.11.5)