##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512926_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2338462 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.63635457835107 31.0 31.0 34.0 27.0 34.0 2 30.760411330181974 31.0 31.0 34.0 27.0 34.0 3 30.869794334909013 31.0 31.0 34.0 27.0 34.0 4 34.37739976103952 37.0 35.0 37.0 31.0 37.0 5 34.063451105897805 37.0 35.0 37.0 30.0 37.0 6 33.953775173597 37.0 35.0 37.0 30.0 37.0 7 33.828802007473286 37.0 35.0 37.0 29.0 37.0 8 33.713416339457304 37.0 35.0 37.0 28.0 37.0 9 35.193397198671605 39.0 35.0 39.0 28.0 39.0 10 35.08189057594265 39.0 35.0 39.0 28.0 39.0 11 35.13511744043735 39.0 35.0 39.0 28.0 39.0 12 34.99081704128611 39.0 35.0 39.0 27.0 39.0 13 34.99384723805647 39.0 35.0 39.0 27.0 39.0 14 36.06953330864474 40.0 36.0 41.0 27.0 41.0 15 36.088986692963154 40.0 36.0 41.0 27.0 41.0 16 36.06681955918035 40.0 36.0 41.0 27.0 41.0 17 36.03378759201561 40.0 36.0 41.0 27.0 41.0 18 35.95554129166948 40.0 36.0 41.0 27.0 41.0 19 35.93785488068654 40.0 36.0 41.0 27.0 41.0 20 35.91313008293485 40.0 36.0 41.0 27.0 41.0 21 35.856038712623935 40.0 36.0 41.0 27.0 41.0 22 35.82651503424045 40.0 36.0 41.0 27.0 41.0 23 35.72219005483091 40.0 36.0 41.0 27.0 41.0 24 35.707448314319414 40.0 36.0 41.0 27.0 41.0 25 35.61065478079182 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6385977196027603 1101 2 0.6727916075189206 1101 3 0.6767727943767916 1101 4 0.49092903195811033 1101 5 0.8379529369183842 1101 6 0.7034165514660273 1101 7 0.7543177359101492 1101 8 0.8182053634013684 1101 9 0.584107789137633 1101 10 0.9506366087804992 1101 11 0.8881863971756516 1101 12 0.9941344800861458 1101 13 1.108698951604012 1101 14 1.1163379220764185 1101 15 1.0792286718448239 1101 16 1.2307675270848932 1101 17 1.1828375814363454 1101 18 0.701756258998742 1101 19 1.0033201849616944 1101 20 1.0027173265193525 1101 21 1.2372615711556563 1101 22 1.027871336135476 1101 23 1.1092544083252065 1101 24 1.1018823185678812 1101 25 1.0804019348695064 1102 1 0.4879905999166958 1102 2 0.42952360650322063 1102 3 0.3818570322696324 1102 4 0.371321663588553 1102 5 0.5090388072698318 1102 6 0.3730058072908662 1102 7 0.3654070394915365 1102 8 0.35471000335775926 1102 9 0.46380569402289495 1102 10 0.49329201557893043 1102 11 0.42770217489104567 1102 12 0.5344286313066746 1102 13 0.6516614999866377 1102 14 0.6754021441513629 1102 15 0.6790721817880865 1102 16 0.6820452914028294 1102 17 0.6308604020778859 1102 18 0.7990252104862421 1102 19 0.741729830736233 1102 20 0.7104329065692241 1102 21 0.7145496194386922 1102 22 0.8405031034422734 1102 23 0.7663457898988639 1102 24 0.7258845270170653 1102 25 0.758893200662655 1103 1 0.5309177531332274 1103 2 0.48907186749550746 1103 3 0.518339893414062 1103 4 0.5100345570955653 1103 5 0.48166559905735795 1103 6 0.5141554514265323 1103 7 0.6049535908793331 1103 8 0.6390875472231485 1103 9 0.6748799251346256 1103 10 0.7901301359223964 1103 11 0.8106272743252845 1103 12 0.8406439746120427 1103 13 0.8239907613486324 1103 14 0.9571689442245415 1103 15 0.7318586890026282 1103 16 0.8722306805191096 1103 17 1.0132050541120137 1103 18 0.9986176560091735 1103 19 1.061671309225332 1103 20 1.1090029324347555 1103 21 0.7808135087409553 1103 22 1.0136325781945033 1103 23 0.9820089408269013 1103 24 1.0462141224007908 1103 25 1.0659505806034915 1104 1 0.5656808242340752 1104 2 0.6445664324246572 1104 3 0.6535686877937579 1104 4 0.5456371783335641 1104 5 0.6080247166110269 1104 6 0.5729692305440395 1104 7 0.6020894367944862 1104 8 0.6144506444361824 1104 9 0.6474720660945295 1104 10 0.7402408888111438 1104 11 0.8190088519966494 1104 12 0.7015258499987027 1104 13 0.8544927604141392 1104 14 0.94258250400528 1104 15 0.9814702597513261 1104 16 0.956283579944234 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0.22607198235585457 2211 1 0.01976228830432092 2211 2 0.08351109459325201 2211 3 -0.1877249523108162 2211 4 -0.06738999000643986 2211 5 0.033993736553007636 2211 6 0.22420056203585403 2211 7 0.19735809979562902 2211 8 0.220775817279538 2211 9 -0.0757894253301643 2211 10 -0.09621545322170988 2211 11 0.08792320003528431 2211 12 0.09724915434618708 2211 13 0.0688395564331401 2211 14 -1.0245238126245866 2211 15 -0.41065355937718806 2211 16 -0.04509226189135518 2211 17 0.010157421304356262 2211 18 -0.14849313165152722 2211 19 -0.05746827630789397 2211 20 -0.1829598672561943 2211 21 -0.3018600214957985 2211 22 -0.06649194582450946 2211 23 -0.19619292195400106 2211 24 -0.2254631536024334 2211 25 -0.34268251657592685 2212 1 -0.06330432982946377 2212 2 -0.0023503368980257733 2212 3 0.08130287289654348 2212 4 0.19739111412415866 2212 5 0.05967181388569287 2212 6 0.13091109582818916 2212 7 0.12453570820839843 2212 8 0.012944010725462363 2212 9 -0.09204226852638442 2212 10 -0.06671597390003825 2212 11 0.06690141030831853 2212 12 0.12132651853803367 2212 13 0.1595025647555559 2212 14 0.03294349097053839 2212 15 0.09608417570865413 2212 16 0.15094875439622513 2212 17 -0.11669765346685068 2212 18 0.26118746271502147 2212 19 0.2614327126714997 2212 20 -0.06668221377985617 2212 21 0.14856386298394142 2212 22 0.1303779082224068 2212 23 -0.42533076753279175 2212 24 0.09312222160945538 2212 25 0.001845353920039372 2213 1 -0.11903977836246327 2213 2 -0.11428515849213383 2213 3 -0.1392893955406116 2213 4 -0.06835690223537227 2213 5 0.1122156097566247 2213 6 0.28847399120708417 2213 7 -0.17020317689751607 2213 8 0.057759575806322516 2213 9 -0.0342812440534388 2213 10 0.16906790049345943 2213 11 0.1594403989072788 2213 12 -0.060057577081117586 2213 13 0.19418384233934205 2213 14 0.1694170437125493 2213 15 -0.001567380802278251 2213 16 -0.20737740932384696 2213 17 -0.184254206550321 2213 18 -0.14912202820114118 2213 19 -0.484780635083645 2213 20 0.04417211078561678 2213 21 -0.7126942240123029 2213 22 -0.14156602098766768 2213 23 -0.3100910888567725 2213 24 -0.33964816536739306 2213 25 -0.36757142545265964 2214 1 -0.8171736784767596 2214 2 -0.746167248663749 2214 3 -0.549595316573054 2214 4 -1.148644472443685 2214 5 -1.0689339031148464 2214 6 -2.2993422009205844 2214 7 -1.0417548043076437 2214 8 -1.620927659646192 2214 9 -0.9982369269941316 2214 10 -1.222927561607861 2214 11 -0.9770006774163207 2214 12 -1.0465159561498751 2214 13 -1.1548225205701215 2214 14 -1.3001019557088043 2214 15 -1.7334814483983365 2214 16 -1.1147649969529283 2214 17 -1.1983699890616606 2214 18 -1.23692114576901 2214 19 -2.7961855912382276 2214 20 -1.529827142969566 2214 21 -2.087679805058002 2214 22 -1.6910764217551275 2214 23 -1.794111267996442 2214 24 -1.1173283480038094 2214 25 -0.8956448344144903 2215 1 -1.215248296575922 2215 2 -1.0500079844346892 2215 3 -1.0558632698953936 2215 4 -0.9779371750326931 2215 5 -1.5859705722805586 2215 6 -1.3848157518809998 2215 7 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2216 19 -2.89748115905865 2216 20 -3.2447068164998143 2216 21 -3.2363584700693835 2216 22 -3.4649125632870863 2216 23 -2.451455849881377 2216 24 -2.8064097708522695 2216 25 -2.5112355954465926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 50261.0 3 3.0 4 777.0 5 6741.0 6 8019.0 7 6286.0 8 5069.0 9 4677.0 10 4080.0 11 3568.0 12 3538.0 13 3335.0 14 3209.0 15 3282.0 16 3419.0 17 3640.0 18 4076.0 19 4940.0 20 5986.0 21 8011.0 22 10545.0 23 12596.0 24 15523.0 25 18082.0 26 20929.0 27 23295.0 28 26599.0 29 32329.0 30 41065.0 31 52753.0 32 66864.0 33 85910.0 34 112370.0 35 146604.0 36 200456.0 37 311077.0 38 1028548.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42468672966311 19.514473774308406 13.624178307313064 27.436661188715423 2 13.89146180944879 20.263438556518867 39.99045524871518 25.85464438531716 3 17.0018584864753 24.2074491695824 30.01288881324563 28.777803530696673 4 11.632897844858194 17.10360952673459 37.38932772339994 33.87416490500728 5 12.975477949326288 37.86496139516611 36.0081976964627 13.151362959044896 6 32.56363324347769 37.32314351239841 17.374591894333534 12.738631349790367 7 28.218027628194925 31.45008666964406 22.109699984428556 18.222185717732454 8 25.16764406433663 37.170098863884874 20.162309497326724 17.499947574451767 9 26.04134308548191 14.954392702832454 19.89178161553977 39.11248259614587 10 14.444291265428028 29.097686159600357 34.74939246918676 21.708630105784856 11 34.52420968182001 22.108895029939763 24.677573446711843 18.689321841528386 12 23.024005303621646 24.645330995177552 31.686701382577176 20.643962318623625 13 28.798991044191425 21.721289215608707 25.73474538107198 23.744974359127884 14 20.72609061784036 21.836093085557952 27.429814390856528 30.008001905745164 15 22.775575001571717 31.001479336124927 23.05814145943202 23.16480420287134 16 22.44428760853758 28.103169511099708 27.209675349672786 22.242867530689935 17 21.758083428623742 28.631099281268625 27.616507763761923 21.994309526345713 18 21.76547663644123 27.622402742355124 29.155227357866053 21.45689326333759 19 23.514589704519516 26.988996834061275 27.303709019311672 22.19270444210754 20 23.654280565170943 27.243991915376608 27.658358800923423 21.44336871852903 21 22.970584009277918 26.68145135080583 26.992383008275546 23.355581631640707 22 23.217186746964767 27.81424300595308 27.004263074776357 21.964307172305794 23 22.323347567760347 27.586593239488177 27.503547203247265 22.586511989504213 24 23.120067805249775 26.799580236924953 27.8802477867932 22.20010417103207 25 22.76480514826317 27.265643776658848 27.64518620458427 22.32436487049371 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 73.0 1 73.0 2 184.5 3 296.0 4 296.0 5 296.0 6 762.5 7 1229.0 8 1229.0 9 1229.0 10 1998.0 11 2767.0 12 2767.0 13 2767.0 14 5536.5 15 8306.0 16 8306.0 17 8306.0 18 16134.0 19 23962.0 20 23962.0 21 23962.0 22 39551.0 23 55140.0 24 55140.0 25 55140.0 26 80597.0 27 106054.0 28 106054.0 29 106054.0 30 137279.5 31 168505.0 32 168505.0 33 168505.0 34 194798.0 35 221091.0 36 221091.0 37 221091.0 38 242084.0 39 263077.0 40 263077.0 41 263077.0 42 277695.0 43 292313.0 44 292313.0 45 292313.0 46 299399.0 47 306485.0 48 306485.0 49 306485.0 50 296704.0 51 286923.0 52 286923.0 53 286923.0 54 258138.5 55 229354.0 56 229354.0 57 229354.0 58 198265.0 59 167176.0 60 167176.0 61 167176.0 62 136533.5 63 105891.0 64 105891.0 65 105891.0 66 81667.5 67 57444.0 68 57444.0 69 57444.0 70 41966.5 71 26489.0 72 26489.0 73 26489.0 74 18388.0 75 10287.0 76 10287.0 77 10287.0 78 7101.0 79 3915.0 80 3915.0 81 3915.0 82 2596.0 83 1277.0 84 1277.0 85 1277.0 86 806.5 87 336.0 88 336.0 89 336.0 90 200.5 91 65.0 92 65.0 93 65.0 94 35.0 95 5.0 96 5.0 97 5.0 98 3.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07791445830635692 2 1.7105259781856623E-4 3 0.0 4 0.030105257216067653 5 0.07303945926852778 6 0.07487827469507737 7 0.03634867703644532 8 0.07761511626017442 9 0.057131567671401116 10 0.11879602918499425 11 0.0042763149454641556 12 0.01868749631167836 13 0.008680919339292235 14 0.011503287203298579 15 0.010733550513115031 16 0.024032889993508555 17 0.0372467031749928 18 0.0014539470814578129 19 0.00577302517637661 20 0.008381577293109744 21 2.5657889672784935E-4 22 3.8486834509177397E-4 23 0.0 24 0.0 25 0.005131577934556987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2338462.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.81707673959767 #Duplication Level Percentage of deduplicated Percentage of total 1 80.61955520802816 56.28621672671134 2 12.82823531855421 17.912597793382332 3 3.5383293096820694 7.4110742683212205 4 1.2952789624679746 3.6173036288725218 5 0.6060192330146073 2.1155245648526484 6 0.3031848345281555 1.2700487315120672 7 0.1889908123595865 0.9236350234711709 8 0.12180201134317022 0.6803088298386769 9 0.08186148310544798 0.5143796503191307 >10 0.38369385664239236 4.628814499951646 >50 0.018089154708507128 0.8738968072457538 >100 0.0125721705474776 1.7402583092281068 >500 0.001469320011277939 0.70235590625268 >1k 9.183250070470361E-4 1.3235852600407378 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3264 0.13957891981995005 No Hit GTATCAACGCAGAGTACTTTTTTTT 3186 0.13624339416248799 No Hit CTGTAGGACGTGGAATATGGCAAGA 3170 0.13555918377121373 No Hit CTTTAGGACGTGAAATATGGCGAGG 3013 0.12884536930683502 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2920 0.12486839640755334 No Hit GTCCTACAGTGTGCATTTCTCATTT 2450 0.1047697161638718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.276314945464156E-5 0.0 0.0 0.0 0.0 6 4.276314945464156E-5 0.0 0.0 0.0 0.0 7 4.276314945464156E-5 0.0 0.0 0.0 0.0 8 4.276314945464156E-5 0.0 0.0 0.0 0.0 9 4.276314945464156E-5 0.0 0.0 0.0 0.0 10 4.276314945464156E-5 0.0 0.0 0.0 0.0 11 4.276314945464156E-5 0.0 0.0 0.0 0.0 12 4.276314945464156E-5 0.0 0.0 0.0 4.276314945464156E-5 13 4.276314945464156E-5 0.0 0.0 0.0 4.276314945464156E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 750 0.0 13.447713 4 CTTCGCA 50 0.0014970143 13.303448 9 CCGATCA 65 5.44153E-5 13.157256 9 GGTATCA 805 0.0 12.634931 1 GGCGAGG 845 0.0 11.45473 19 ACCGATC 60 0.0058592893 11.087869 8 GACGTGA 1030 0.0 10.980925 7 AGGACGT 1800 0.0 10.677821 5 GGACGTG 1755 0.0 10.622673 6 TCCAACG 90 9.6287E-5 10.543616 18 GTAGGAC 1840 0.0 10.54053 3 CCTACAC 355 0.0 10.4444685 3 AGGACCT 1430 0.0 10.379881 5 TTAGGAC 1320 0.0 10.37143 3 TGTAGGA 1900 0.0 10.157198 2 GTCCTAC 2070 0.0 10.102697 1 GGACCTG 1370 0.0 9.9976225 6 GTATCAA 1675 0.0 9.988099 1 TAGGACG 1945 0.0 9.979629 4 ACGTGAA 1210 0.0 9.975154 8 >>END_MODULE