FastQCFastQC Report
Thu 26 May 2016
SRR1512925_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512925_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451919
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68650.2799847792688095No Hit
TATCAACGCAGAGTACTTTTTTTTT40770.1662779235366258No Hit
GGTATCAACGCAGAGTACTTTTTTT39020.15914065676720968No Hit
GTCCTACAGTGGACATTTCTAAATT31090.12679864220636977No Hit
CTGTAGGACGTGGAATATGGCAAGA26750.10909822061821782No Hit
GTCCTAAAGTGTGTATTTCTCATTT26720.10897586747359925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG707.2923976E-613.5667795
GGTATCA12200.012.3438961
GCCGGTT1201.284534E-711.0781811
TAGGACC6800.011.0327594
GTATCAA25700.010.9035831
TGTAGGA20250.010.7862072
GTAGGAC19950.010.6151933
GTCTTAT2350.010.5453371
CCAACGA1451.4564648E-810.47724419
GATATAC4700.010.3425421
GGTCTAA1201.4614434E-610.3256431
GTACTAA1951.4551915E-1110.26454451
TAGGACA7050.010.2374174
GGACGTG17500.010.1458816
GAAGCGT750.002660541610.1277946
TAGGACT3850.010.1132114
CTGTAGG21200.010.0708741
GGACCGT856.629065E-410.0533266
AGGACGT18000.010.0243435
GGTATAC1356.162136E-79.8843761
Produced by FastQC (version 0.11.5)