##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512925_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2451919 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.016418976320182 31.0 30.0 34.0 23.0 34.0 2 29.128421044904012 31.0 30.0 34.0 22.0 34.0 3 29.333995535741597 31.0 30.0 34.0 23.0 34.0 4 32.764280141391296 35.0 33.0 37.0 25.0 37.0 5 32.90930777077057 37.0 35.0 37.0 26.0 37.0 6 32.913213283146796 37.0 35.0 37.0 26.0 37.0 7 32.7555249582062 37.0 35.0 37.0 25.0 37.0 8 32.6993758766093 37.0 35.0 37.0 25.0 37.0 9 34.018368061913954 39.0 35.0 39.0 24.0 39.0 10 33.921339571168545 39.0 34.0 39.0 23.0 39.0 11 33.95042780777016 39.0 35.0 39.0 23.0 39.0 12 33.85483574294257 39.0 34.0 39.0 23.0 39.0 13 33.83634288082111 39.0 34.0 39.0 22.0 39.0 14 34.840293663860834 39.0 34.0 41.0 22.0 41.0 15 34.77994093605866 39.0 34.0 41.0 20.0 41.0 16 34.664315583018855 39.0 34.0 41.0 19.0 41.0 17 34.61703547303153 39.0 34.0 41.0 18.0 41.0 18 34.3228585446746 39.0 34.0 41.0 17.0 41.0 19 34.55888918027064 39.0 34.0 41.0 18.0 41.0 20 34.63457357278116 39.0 34.0 41.0 18.0 41.0 21 34.572252590725874 39.0 34.0 41.0 18.0 41.0 22 34.4889741463727 39.0 34.0 41.0 18.0 41.0 23 34.22257749950141 39.0 34.0 41.0 11.0 41.0 24 34.22837948561922 39.0 34.0 41.0 10.0 41.0 25 34.149332013006955 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49088300537449214 1101 2 0.7230119809498952 1101 3 0.7481723286093072 1101 4 0.675459419199619 1101 5 0.5066533353595304 1101 6 0.7620957870609573 1101 7 0.7974441498602687 1101 8 0.5894943103831309 1101 9 0.5212570510447208 1101 10 0.6032671451845815 1101 11 0.7368889958705438 1101 12 0.9691707074072156 1101 13 0.6843655587094304 1101 14 0.6692106479905036 1101 15 0.521212380761618 1101 16 1.0111330535558523 1101 17 0.9285729243914318 1101 18 1.1088345868738898 1101 19 0.5728958475917381 1101 20 0.9264976536643204 1101 21 0.7683538066388991 1101 22 0.7920958358877854 1101 23 1.0032809188080805 1101 24 0.6667753316831835 1101 25 0.911282917560051 1102 1 0.7422420334438016 1102 2 0.8545754503100724 1102 3 0.788072051651838 1102 4 1.0285203664620894 1102 5 0.9615633151495118 1102 6 0.8519977139132564 1102 7 1.0438449672207994 1102 8 1.0338081560167325 1102 9 1.0226359856580487 1102 10 1.0326692190779596 1102 11 1.136283345591309 1102 12 1.193578370306689 1102 13 1.037460321807103 1102 14 1.0793059754079906 1102 15 1.3104980356941454 1102 16 1.2738862839435754 1102 17 0.9885516666591272 1102 18 1.4373278240681273 1102 19 1.4127918855753023 1102 20 1.3373272471445077 1102 21 1.1702588376114562 1102 22 1.3921990175387933 1102 23 1.5271895091140593 1102 24 1.5210472182238277 1102 25 1.441132256453244 1103 1 0.6050926109434371 1103 2 0.7399802504862123 1103 3 0.7428110881468051 1103 4 0.7842022424423263 1103 5 0.4492111563801373 1103 6 0.7076864876993696 1103 7 0.6139268838911249 1103 8 0.6573294650112658 1103 9 0.9665134165249611 1103 10 0.9447381497577965 1103 11 0.9365729140882664 1103 12 1.1087495717581533 1103 13 1.1560743644857112 1103 14 1.2648626666629 1103 15 1.304571426955114 1103 16 1.3676820907743519 1103 17 1.1470150306006062 1103 18 1.4718847166832276 1103 19 1.425621943844341 1103 20 1.317214778071417 1103 21 1.2134662058616215 1103 22 1.1417996067089575 1103 23 1.497592069245158 1103 24 1.3151723081914213 1103 25 1.3566118385994983 1104 1 0.5501283127323333 1104 2 0.6170047083409287 1104 3 0.5694787069895995 1104 4 0.7394223760689655 1104 5 0.5508262369786792 1104 6 0.6265304660728575 1104 7 0.7631934949958392 1104 8 0.722556969747032 1104 9 0.8510404581206075 1104 10 0.9403740321024046 1104 11 0.8517684204774056 1104 12 0.7466111217715508 1104 13 0.9407920201840483 1104 14 0.9826931140907362 1104 15 1.1047301596692307 1104 16 0.9773171912887904 1104 17 0.916396733930867 1104 18 1.2042483026610853 1104 19 1.0749435252327686 1104 20 1.062722797747334 1104 21 1.1208381818596322 1104 22 1.0817847158851706 1104 23 1.2631237199511816 1104 24 1.0971819034298846 1104 25 0.8632018055526558 1105 1 0.658093522794605 1105 2 0.7486536356014994 1105 3 0.7285306308677413 1105 4 0.825438361292754 1105 5 0.5659429156028821 1105 6 0.3558322703414518 1105 7 0.6517902024822178 1105 8 0.7767126479909763 1105 9 0.5079571856688503 1105 10 0.6967399335550724 1105 11 0.6582262835253943 1105 12 0.6769498885714142 1105 13 0.6170802311464527 1105 14 0.7762468188830098 1105 15 0.9537051683485274 1105 16 0.9344005723086966 1105 17 0.8926305651052147 1105 18 0.9776852461040519 1105 19 0.8401976574198144 1105 20 0.8966360271861475 1105 21 0.8172560756950276 1105 22 0.5579908580801813 1105 23 0.8609720606429434 1105 24 0.8393910792431427 1105 25 0.9771441432873758 1106 1 0.6395285344988935 1106 2 0.6995861113202331 1106 3 0.755694928569131 1106 4 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1107 17 1.024740879744897 1107 18 1.2178583280469084 1107 19 1.1538086848679612 1107 20 0.9484855141648438 1107 21 0.9336805882745907 1107 22 0.9455588760710683 1107 23 1.0552001191936355 1107 24 0.9933372270655596 1107 25 0.9842091795301684 1108 1 0.4950733101131597 1108 2 0.6120652574427048 1108 3 0.47392821859099143 1108 4 0.6898553098501239 1108 5 0.5912138744852271 1108 6 0.7779740922399299 1108 7 0.75039960845222 1108 8 0.7250806193325872 1108 9 0.8870155605764722 1108 10 0.7996043035719111 1108 11 0.7852660940360892 1108 12 0.8428487577374071 1108 13 0.8329252590217564 1108 14 1.127422414925789 1108 15 0.9991220494791051 1108 16 0.8349790407325983 1108 17 1.018729338004455 1108 18 1.1238705255680586 1108 19 1.073455219066929 1108 20 1.0267476952914762 1108 21 0.8796986878220991 1108 22 0.8034807070107988 1108 23 1.0759083623571257 1108 24 1.2551025887286187 1108 25 1.3653895848026494 1109 1 0.01485943060163919 1109 2 0.10297377890462656 1109 3 0.2565665012643237 1109 4 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0.9081106193741988 1110 17 0.6824057472684188 1110 18 1.1196623897096316 1110 19 1.0032461614079153 1110 20 0.8416787990895784 1110 21 0.9237287380047192 1110 22 0.9160134509426001 1110 23 0.9459646903115626 1110 24 1.117142350093296 1110 25 1.1571459247637321 1111 1 0.0806548979548829 1111 2 0.2668543310055931 1111 3 0.255180103613732 1111 4 0.12570597412199191 1111 5 0.1470343513435708 1111 6 0.3400441210883898 1111 7 0.2546773759361045 1111 8 0.05817641162370535 1111 9 0.41276344968911616 1111 10 0.23081946868975933 1111 11 0.20633371681785206 1111 12 0.1288955996227159 1111 13 0.23615945086601187 1111 14 0.266979963740269 1111 15 0.3361047417581915 1111 16 0.25529157243141043 1111 17 0.2504840084652855 1111 18 0.4652959882185712 1111 19 0.2370313726332185 1111 20 0.21705756259940046 1111 21 0.406133502890377 1111 22 0.16010212411510594 1111 23 0.5596324504794836 1111 24 0.6047781957566656 1111 25 0.07852394989993883 1112 1 -0.2731638075424243 1112 2 0.02886791324860738 1112 3 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2214 5 -1.4139682641751712 2214 6 -1.5059168319323213 2214 7 -1.4955643094298559 2214 8 -1.3163101320325872 2214 9 -1.461921330278578 2214 10 -1.5214816161073585 2214 11 -1.3014899714923516 2214 12 -1.9113739560249456 2214 13 -1.7283866923157731 2214 14 -2.7489555487155997 2214 15 -2.863896544562504 2214 16 -2.253393807819279 2214 17 -2.675501670497656 2214 18 -2.0719129817244024 2214 19 -4.149801848742573 2214 20 -2.4688395031601686 2214 21 -2.278107353513356 2214 22 -2.4253836048714135 2214 23 -2.524903830910315 2214 24 -2.788838795110461 2214 25 -2.5515496562286657 2215 1 -0.9134846800210603 2215 2 -2.814448856290433 2215 3 -1.4502733078380956 2215 4 -1.064023183155431 2215 5 -0.901914495366789 2215 6 -1.4734237847954752 2215 7 -1.9456149405358936 2215 8 -1.95767788500984 2215 9 -1.5596586190185064 2215 10 -3.0468363904067246 2215 11 -2.00059857878038 2215 12 -2.2866460090806413 2215 13 -1.6713874767413586 2215 14 -3.022988209344568 2215 15 -2.810590547272337 2215 16 -2.511305096416578 2215 17 -2.6925265581821733 2215 18 -1.6432457921073436 2215 19 -2.2055573932052397 2215 20 -2.4407114278553124 2215 21 -2.2355116179360905 2215 22 -1.8508046987885507 2215 23 -2.3003834001893537 2215 24 -2.0556148059497943 2215 25 -2.085070300883878 2216 1 -2.0222254872966445 2216 2 -2.090690224953768 2216 3 -2.514809741903104 2216 4 -3.2677907966961044 2216 5 -2.3983328415184744 2216 6 -2.4733550147960344 2216 7 -2.207743722885862 2216 8 -2.769551937668009 2216 9 -2.9626171324637447 2216 10 -2.585505480397181 2216 11 -3.020128556886256 2216 12 -3.5804840767840034 2216 13 -4.021378354190414 2216 14 -3.4413462905212384 2216 15 -3.359458649220354 2216 16 -2.478390932603638 2216 17 -2.611144281193461 2216 18 -2.689351164588114 2216 19 -2.9138645284435505 2216 20 -2.8861080483010575 2216 21 -3.2062212120527462 2216 22 -3.730143524365374 2216 23 -3.538302335798182 2216 24 -3.625821256295051 2216 25 -3.6194651248888157 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 118429.0 3 3.0 4 970.0 5 5810.0 6 7336.0 7 6815.0 8 6155.0 9 5786.0 10 5245.0 11 5030.0 12 4843.0 13 4636.0 14 4552.0 15 4683.0 16 4855.0 17 5176.0 18 5698.0 19 6850.0 20 8896.0 21 11275.0 22 14102.0 23 17173.0 24 20256.0 25 22426.0 26 25015.0 27 27918.0 28 31391.0 29 37324.0 30 45851.0 31 57030.0 32 72044.0 33 92001.0 34 118995.0 35 156560.0 36 214706.0 37 330396.0 38 945688.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.16271115957262 18.722310631735088 12.909876324914782 25.20510188377751 2 14.973449233261185 19.952671702778293 41.10735975421926 23.966519309741265 3 18.549939760338763 24.306876705141445 29.833462734027922 27.30972080049187 4 12.25960194868326 16.648259897327353 38.28025968844078 32.8118784655486 5 13.21814998614813 37.68719911968218 35.87735772458897 13.217293169580726 6 32.05907739600636 37.19712371547822 17.77453440674592 12.9692644817695 7 27.763827192220305 31.739050625940745 22.265705626328263 18.231416555510684 8 24.890010439064866 36.67436440617467 20.71103942327736 17.724585731483103 9 25.99401901823298 15.321340095625546 20.32970865659611 38.35493222954536 10 14.762862021618298 28.833478418008667 34.70936697666108 21.694292583711956 11 34.01949278616797 22.68641970474054 24.769536331451604 18.524551177639875 12 22.86010320422607 24.92320871321055 31.71086499826633 20.50582308429705 13 28.541096681108453 22.158223843756208 25.929745281467998 23.370934193667342 14 20.867147313708475 22.057342309871704 27.793001123087173 29.282509253332652 15 22.85744591013954 30.732913274682865 23.586748774602295 22.822892040575297 16 22.32241342174997 28.32741296487266 27.367277141775553 21.982896471601812 17 21.618258954067063 28.59508852084523 27.84873949579832 21.937913029289387 18 21.527054216218158 27.690968536367883 29.42754349807129 21.35443374934267 19 23.10944218264296 27.26977897802834 27.799040524774206 21.82173831455449 20 23.244485781536188 27.417016468207834 28.150722651230524 21.18777509902545 21 22.78446466488395 26.979886305921514 27.55684193993836 22.678807089256175 22 22.944650036549707 27.774188074352544 27.580140128968257 21.701021760129493 23 22.294036324682597 27.58389012605496 27.911540835386926 22.210532713875512 24 22.78549532222306 26.838100171936603 28.333234753628922 22.043169752211416 25 22.50523068664057 27.285480658187428 28.11476222353032 22.094526431641686 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 422.0 1 422.0 2 738.0 3 1054.0 4 1054.0 5 1054.0 6 2227.5 7 3401.0 8 3401.0 9 3401.0 10 4961.5 11 6522.0 12 6522.0 13 6522.0 14 10412.0 15 14302.0 16 14302.0 17 14302.0 18 24119.5 19 33937.0 20 33937.0 21 33937.0 22 50702.0 23 67467.0 24 67467.0 25 67467.0 26 93233.0 27 118999.0 28 118999.0 29 118999.0 30 149701.5 31 180404.0 32 180404.0 33 180404.0 34 203114.0 35 225824.0 36 225824.0 37 225824.0 38 245581.0 39 265338.0 40 265338.0 41 265338.0 42 279676.0 43 294014.0 44 294014.0 45 294014.0 46 301107.5 47 308201.0 48 308201.0 49 308201.0 50 301162.5 51 294124.0 52 294124.0 53 294124.0 54 267392.0 55 240660.0 56 240660.0 57 240660.0 58 208429.0 59 176198.0 60 176198.0 61 176198.0 62 144186.5 63 112175.0 64 112175.0 65 112175.0 66 87109.5 67 62044.0 68 62044.0 69 62044.0 70 45535.5 71 29027.0 72 29027.0 73 29027.0 74 20364.0 75 11701.0 76 11701.0 77 11701.0 78 7968.5 79 4236.0 80 4236.0 81 4236.0 82 2795.0 83 1354.0 84 1354.0 85 1354.0 86 874.5 87 395.0 88 395.0 89 395.0 90 242.5 91 90.0 92 90.0 93 90.0 94 52.5 95 15.0 96 15.0 97 15.0 98 15.0 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35657784780002927 2 0.038908299988702724 3 0.03572711822862012 4 0.03376946791472312 5 0.04021340019796739 6 0.017251793391217247 7 0.016680812049663957 8 0.02243140984673637 9 0.01965807190204897 10 0.019454149994351362 11 0.018271402929705263 12 0.019127874942035196 13 0.019087090560495675 14 0.026102004185293234 15 0.027488673157636936 16 0.020188268862062736 17 0.02116709401901123 18 0.10665115772584656 19 0.02361415691138247 20 0.02157493783440644 21 0.02463376644987049 22 0.018883168652798075 23 0.021534153452866918 24 0.017700421588151976 25 0.021207878400550752 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2451919.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.04196262236378 #Duplication Level Percentage of deduplicated Percentage of total 1 79.52217877678744 54.10845115979102 2 13.145813830775586 17.889339466283705 3 3.8822456879968734 7.924668479805487 4 1.4839427936189122 4.038815203885765 5 0.6657448247459133 2.2649292240696783 6 0.3678681068351818 1.5018280785143499 7 0.2321199987280137 1.1055730194148246 8 0.13806378664583063 0.7515304808366091 9 0.09787546069904879 0.5993674594678182 >10 0.4280438365019558 4.94577118915875 >50 0.02128596285341681 0.9873904183082829 >100 0.012171590594638307 1.5967349102566923 >500 0.001743521665847809 0.7646896086542678 >1k 8.417001145389072E-4 1.240079216634954 >5k 6.012143675277909E-5 0.28083208491782535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6865 0.2799847792688095 No Hit TATCAACGCAGAGTACTTTTTTTTT 4077 0.1662779235366258 No Hit GGTATCAACGCAGAGTACTTTTTTT 3902 0.15914065676720968 No Hit GTCCTACAGTGGACATTTCTAAATT 3109 0.12679864220636977 No Hit CTGTAGGACGTGGAATATGGCAAGA 2675 0.10909822061821782 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2672 0.10897586747359925 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 4.0784381539520675E-5 0.0 0.0 0.0 11 0.0 4.0784381539520675E-5 0.0 0.0 0.0 12 0.0 4.0784381539520675E-5 0.0 0.0 0.0 13 0.0 4.0784381539520675E-5 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACCG 70 7.2923976E-6 13.566779 5 GGTATCA 1220 0.0 12.343896 1 GCCGGTT 120 1.284534E-7 11.07818 11 TAGGACC 680 0.0 11.032759 4 GTATCAA 2570 0.0 10.903583 1 TGTAGGA 2025 0.0 10.786207 2 GTAGGAC 1995 0.0 10.615193 3 GTCTTAT 235 0.0 10.545337 1 CCAACGA 145 1.4564648E-8 10.477244 19 GATATAC 470 0.0 10.342542 1 GGTCTAA 120 1.4614434E-6 10.325643 1 GTACTAA 195 1.4551915E-11 10.2645445 1 TAGGACA 705 0.0 10.237417 4 GGACGTG 1750 0.0 10.145881 6 GAAGCGT 75 0.0026605416 10.127794 6 TAGGACT 385 0.0 10.113211 4 CTGTAGG 2120 0.0 10.070874 1 GGACCGT 85 6.629065E-4 10.053326 6 AGGACGT 1800 0.0 10.024343 5 GGTATAC 135 6.162136E-7 9.884376 1 >>END_MODULE