FastQCFastQC Report
Thu 26 May 2016
SRR1512925_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512925_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2451919
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT83380.3400601732765234No Hit
TATCAACGCAGAGTACTTTTTTTTT60060.2449509955263612No Hit
GGTATCAACGCAGAGTACTTTTTTT52380.2136285905040093No Hit
GTCCTACAGTGGACATTTCTAAATT28920.11794843141229379No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28030.11431862145527646No Hit
CTTTAGGACGTGAAATATGGCGAGG26830.10942449567053399No Hit
CTGTAGGACGTGGAATATGGCAAGA26810.10934292690745495No Hit
GTCCTAAAGTGTGTATTTCTCATTT25840.10538684189812142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGT759.547784E-713.9502426
GGTATCA13800.012.5372711
TAGGACC6300.012.5356734
TAGCACC1855.456968E-1210.8008434
CTAGCAC2750.010.7172673
TCGAACT1909.094947E-1210.48851619
GTAGGAC18500.010.3808933
TGGCGAG16950.010.30119518
CTGTAGG19300.010.2943681
TGTAGGA19700.010.2794162
GTATCAA28000.010.2192531
GTATTAA3550.010.1757381
GGCGAGG7950.010.14612419
AGGACGT17350.010.1457085
GGACGTG17250.010.145636
TTAGGAC11750.09.9522723
TAGGACG18600.09.9242774
GACCGTG1554.041067E-89.810337
TAGGACT4400.09.7312794
TATGGCG18100.09.65004516
Produced by FastQC (version 0.11.5)