##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512925_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2451919
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.327155587113605	31.0	31.0	34.0	26.0	34.0
2	30.482481680675424	31.0	31.0	34.0	27.0	34.0
3	30.59535204874223	31.0	31.0	34.0	27.0	34.0
4	34.06563022677339	37.0	35.0	37.0	30.0	37.0
5	33.69434634667785	37.0	35.0	37.0	28.0	37.0
6	33.549486748950514	37.0	35.0	37.0	28.0	37.0
7	33.414349739938395	37.0	35.0	37.0	27.0	37.0
8	33.30208991406323	37.0	35.0	37.0	27.0	37.0
9	34.72250266016128	39.0	35.0	39.0	27.0	39.0
10	34.61591267900775	39.0	35.0	39.0	27.0	39.0
11	34.633700379172396	39.0	35.0	39.0	27.0	39.0
12	34.48272964971518	39.0	35.0	39.0	27.0	39.0
13	34.46035166740826	39.0	35.0	39.0	27.0	39.0
14	35.51385261911181	40.0	36.0	41.0	26.0	41.0
15	35.52422531086875	40.0	36.0	41.0	26.0	41.0
16	35.49733779949501	40.0	36.0	41.0	26.0	41.0
17	35.483260662362824	40.0	36.0	41.0	26.0	41.0
18	35.41277342359189	40.0	36.0	41.0	26.0	41.0
19	35.40056665819711	40.0	36.0	41.0	25.0	41.0
20	35.3731501733948	40.0	36.0	41.0	25.0	41.0
21	35.32284182307817	40.0	36.0	41.0	25.0	41.0
22	35.2766017148201	40.0	36.0	41.0	25.0	41.0
23	35.17279649123809	40.0	36.0	41.0	25.0	41.0
24	35.157620215023414	40.0	36.0	41.0	25.0	41.0
25	35.062576292283715	40.0	36.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4402139558455893
1101	2	0.48203076437078707
1101	3	0.4931684157979035
1101	4	0.3758924780980095
1101	5	0.6358809363350844
1101	6	0.536522851343527
1101	7	0.6154118512763134
1101	8	0.5880203589365394
1101	9	0.45984286415898623
1101	10	0.7545855266105193
1101	11	0.7118472797290067
1101	12	0.9240108092724029
1101	13	0.934456193820985
1101	14	1.0512129447524217
1101	15	0.9291628688427451
1101	16	1.0841642065028552
1101	17	0.9112331705270904
1101	18	0.41851932380784973
1101	19	0.77783697377469
1101	20	0.6680695863898336
1101	21	0.8103491003843146
1101	22	0.7914330879311535
1101	23	0.7365528588412431
1101	24	0.7586651823787136
1101	25	0.763452093992484
1102	1	0.7204295245694112
1102	2	0.6992924476334643
1102	3	0.6841765106440967
1102	4	0.6219650361864595
1102	5	0.7150744431166771
1102	6	0.7305923631165712
1102	7	0.787720656327302
1102	8	0.8000587962692194
1102	9	0.8929888857042982
1102	10	0.942305175995223
1102	11	0.908648720803825
1102	12	0.9735442157770393
1102	13	1.084827698343851
1102	14	1.247059666531193
1102	15	1.2079049197731493
1102	16	1.1819106123058845
1102	17	1.0457110157358258
1102	18	1.0754468372528976
1102	19	1.163231639976992
1102	20	1.104620024369929
1102	21	1.1504680483072036
1102	22	1.1030944931525184
1102	23	1.1176366563595366
1102	24	1.1575649393815866
1102	25	1.1262569152057083
1103	1	0.5558996448768987
1103	2	0.5255959413373006
1103	3	0.5359250347575006
1103	4	0.5470931159000187
1103	5	0.6022748327381962
1103	6	0.6862850386918566
1103	7	0.6322128280143886
1103	8	0.6284438945002506
1103	9	0.74848082816127
1103	10	0.8170399065871337
1103	11	0.7619756579899004
1103	12	0.77506075762129
1103	13	0.7000301837835607
1103	14	0.8806831954347416
1103	15	0.6955438426285099
1103	16	0.8377800528659591
1103	17	0.9355702656586615
1103	18	0.9738330990531949
1103	19	1.0625930006981008
1103	20	0.9842896821760547
1103	21	0.7264865372139298
1103	22	0.8636579254449828
1103	23	0.900870807605429
1103	24	0.9273122079549765
1103	25	0.8806160846541431
1104	1	0.4687091382380153
1104	2	0.49095778271384205
1104	3	0.4504197560278911
1104	4	0.4507292453700984
1104	5	0.5220375509973962
1104	6	0.5205733689065397
1104	7	0.4981967899009234
1104	8	0.42247168620979636
1104	9	0.6116839404012282
1104	10	0.7086504093099819
1104	11	0.6811881230211654
1104	12	0.5668216271177045
1104	13	0.742202023229602
1104	14	0.8946877523955834
1104	15	0.786283200512301
1104	16	0.7147632314087531
1104	17	0.7397019257488466
1104	18	0.7484574125954353
1104	19	0.7269532546098958
1104	20	0.7037614638448204
1104	21	0.6102695192121601
1104	22	0.7102562968660493
1104	23	0.6098929378573459
1104	24	0.7166770885896554
1104	25	0.8579798859724121
1105	1	0.39469225766885785
1105	2	0.4769031302747955
1105	3	0.38526369068518207
1105	4	0.32206252373568134
1105	5	0.31793647815541703
1105	6	0.41406815428550203
1105	7	0.5331606554410513
1105	8	0.7083982052647997
1105	9	0.7331994532715456
1105	10	0.5180212213518587
1105	11	0.5963003440615324
1105	12	0.5613646420496536
1105	13	0.5931815089092041
1105	14	0.7434122612717147
1105	15	0.6966977981969009
1105	16	0.7002114763308356
1105	17	0.4768054588762851
1105	18	0.6922397599891639
1105	19	0.7483303772778171
1105	20	0.8146600648881943
1105	21	0.7404350008588807
1105	22	0.7269621178005323
1105	23	0.7272659059477462
1105	24	0.7486046011662637
1105	25	0.8569289353789813
1106	1	0.4774690260553456
1106	2	0.3800168732534779
1106	3	0.4248107203046665
1106	4	0.4177325669753742
1106	5	0.5186483936369228
1106	6	0.5447485878663585
1106	7	0.4368634395323241
1106	8	0.5242808315930247
1106	9	0.48859371392369866
1106	10	0.5651867733985867
1106	11	0.4267395396712672
1106	12	0.5080494113848957
1106	13	0.3545465752831731
1106	14	0.6320173872636587
1106	15	0.5969642043498524
1106	16	0.6494780161812912
1106	17	0.5646264971193204
1106	18	0.7823020939086263
1106	19	0.7013105108046389
1106	20	0.8103578364746156
1106	21	0.7044155127211837
1106	22	0.7001369403533317
1106	23	0.7988796103884894
1106	24	0.7134699276882017
1106	25	0.7759084536534786
1107	1	0.39752943615208025
1107	2	0.39377520625415485
1107	3	0.39378747439299033
1107	4	0.42235407243757095
1107	5	0.4565598542572573
1107	6	0.3402033268654634
1107	7	0.404086704072661
1107	8	0.5151251092665703
1107	9	0.37880674600491915
1107	10	0.5755412502657791
1107	11	0.42669469781532143
1107	12	0.4020234377045355
1107	13	0.5308489185030041
1107	14	0.5590667399175757
1107	15	0.5992311797200998
1107	16	0.5405755106259562
1107	17	0.3948533676367987
1107	18	0.5212912227524527
1107	19	0.6209858398001558
1107	20	0.6257517661913283
1107	21	0.5469844055132214
1107	22	0.5446152712250267
1107	23	0.5849576112667023
1107	24	0.6152130384089958
1107	25	0.54519590724815
1108	1	0.491286403822091
1108	2	0.380499581623031
1108	3	0.4603079019620111
1108	4	0.458149206544725
1108	5	0.5525740191205273
1108	6	0.570131190987702
1108	7	0.5295775559318017
1108	8	0.6176000256545251
1108	9	0.5805681729837389
1108	10	0.6568770755658022
1108	11	0.7004313362834154
1108	12	0.7498370648276236
1108	13	0.762532963358403
1108	14	0.8319816851598887
1108	15	0.8666578962261013
1108	16	0.7619637983825314
1108	17	0.812249918230286
1108	18	0.8515850125091617
1108	19	0.8694362299896738
1108	20	0.7768764301037194
1108	21	0.8544380090282004
1108	22	0.8905632022368906
1108	23	0.8962855671982339
1108	24	0.9134293148861445
1108	25	0.9456759387256213
1109	1	0.10569608277625875
1109	2	0.16428789315037662
1109	3	0.15236272058157851
1109	4	0.2788559898357832
1109	5	0.26162098385199783
1109	6	0.026677719243970444
1109	7	0.17705459600355056
1109	8	0.3209101736029609
1109	9	0.377217242925056
1109	10	0.2926483172477816
1109	11	0.23057078177999557
1109	12	0.28219593673581755
1109	13	0.2895032657190981
1109	14	0.3948134562896186
1109	15	0.48340041850632787
1109	16	0.2699410990066582
1109	17	0.38564878725973273
1109	18	0.3202015793751585
1109	19	0.5004714158881285
1109	20	0.5190909786550861
1109	21	0.5086596904506209
1109	22	0.5721635402219647
1109	23	0.413198281487567
1109	24	0.036901685006448304
1109	25	0.23825559378360595
1110	1	0.31425240693865675
1110	2	0.3813502460553444
1110	3	0.3159450223809266
1110	4	0.38316821706796844
1110	5	0.33393880039945856
1110	6	0.3279144025977416
1110	7	0.35899564672011763
1110	8	0.5336503296292463
1110	9	0.6602556386905576
1110	10	0.6504385437849791
1110	11	0.512187779527487
1110	12	0.654143061308261
1110	13	0.34212721617706165
1110	14	0.6177250192118962
1110	15	0.7313761725364714
1110	16	0.7151577599431889
1110	17	0.6463241347966147
1110	18	0.5449616041966863
1110	19	0.7329329268709159
1110	20	0.652093033875353
1110	21	0.7163660956722921
1110	22	0.592134136341059
1110	23	0.7488173557624904
1110	24	0.6653288825177768
1110	25	0.7082541917198597
1111	1	-0.42704988017763057
1111	2	-0.20984436824855024
1111	3	-0.13056987282527643
1111	4	-0.11294891472157786
1111	5	0.17408985466404658
1111	6	-0.1574200985369174
1111	7	0.03584320246586259
1111	8	0.14500673478013226
1111	9	-0.23485499856111147
1111	10	-0.11051700137153375
1111	11	0.17686364925873477
1111	12	0.0357555561061389
1111	13	0.11291172613429268
1111	14	0.2958859510255252
1111	15	0.28909531347106565
1111	16	-0.013526239906255455
1111	17	0.2808813815759734
1111	18	0.16558131482150173
1111	19	0.21342261860393563
1111	20	0.23659530305284449
1111	21	0.10237986850254543
1111	22	-0.002065226137652587
1111	23	0.035235445542419086
1111	24	0.07557724673724664
1111	25	0.07514099124928464
1112	1	-0.12985168063657682
1112	2	-0.2784764559077395
1112	3	-0.11531985753301299
1112	4	-0.2982142521163951
1112	5	-0.13124254083418663
1112	6	-0.034277769536636526
1112	7	-0.2345813962291885
1112	8	-0.10687224705753806
1112	9	-0.25491695698269723
1112	10	0.02981098412258376
1112	11	-0.06854280555503323
1112	12	0.043702040370568795
1112	13	-0.052374590483523775
1112	14	0.027671576134622455
1112	15	-0.02986957589771322
1112	16	-0.23397253259354045
1112	17	-0.05605764833705251
1112	18	-0.037838008279827307
1112	19	-0.018394540441981633
1112	20	0.2118021012542286
1112	21	0.2671397379127143
1112	22	0.14934324223665385
1112	23	0.3250886113258886
1112	24	0.18505080088589665
1112	25	0.2021880354072394
1113	1	-0.10224106084063678
1113	2	-0.5335166582599307
1113	3	-0.09775415270419074
1113	4	-0.07120322417528513
1113	5	-0.02700456486271463
1113	6	-0.15985365319151867
1113	7	0.04451801144841028
1113	8	0.1565828490576422
1113	9	0.1186049416364483
1113	10	-0.12317986401089343
1113	11	0.10051869275042691
1113	12	-0.13479280161170237
1113	13	0.042715806758778285
1113	14	0.052658066286682015
1113	15	0.20150325949217773
1113	16	-0.18744759795270483
1113	17	0.07149312705433886
1113	18	0.17495931392054587
1113	19	0.06740321871753707
1113	20	0.13812910079088425
1113	21	-0.07245291642410479
1113	22	-0.23393094593230757
1113	23	0.07241883317236386
1113	24	-0.17862203598163262
1113	25	-0.24149450110892445
1114	1	-0.5154545487902489
1114	2	-0.38294779642262
1114	3	-0.436197025063187
1114	4	-0.19935072686946853
1114	5	-0.4489171782352699
1114	6	-0.7139581132522892
1114	7	-0.5557364724967684
1114	8	-0.8955417397594374
1114	9	-0.6334151071567362
1114	10	-0.68514318341078
1114	11	-0.7276238318473816
1114	12	-0.5714284943275345
1114	13	-0.5642557101677923
1114	14	-1.6901939883474668
1114	15	-0.9395134716153564
1114	16	-0.8697307228070201
1114	17	-1.0522444486101108
1114	18	-1.1082083311184476
1114	19	-1.0346067954876759
1114	20	-0.3794246339050602
1114	21	-0.4072367572880822
1114	22	-0.46043204451063247
1114	23	-0.47588019963968975
1114	24	-1.2538510022831844
1114	25	-1.0533957576274915
1115	1	-1.5573326828609808
1115	2	-1.4144468547037548
1115	3	-1.101442327251906
1115	4	-2.292702370071705
1115	5	-1.477845080430697
1115	6	-1.3339451975660808
1115	7	-1.0110132798009062
1115	8	-0.952402708827286
1115	9	-1.2935340054867766
1115	10	-1.2867212880344923
1115	11	-1.123411736630736
1115	12	-1.6192005584251206
1115	13	-1.069526021022881
1115	14	-1.922437049793757
1115	15	-1.9431071528069097
1115	16	-1.3503775614591902
1115	17	-1.3304014299764901
1115	18	-1.2032290159187298
1115	19	-1.1401002753425615
1115	20	-1.1625444763501989
1115	21	-1.0862981295617686
1115	22	-1.5596598456916055
1115	23	-1.8739667557708728
1115	24	-1.2697490644776366
1115	25	-1.1630237347450816
1116	1	-0.9315092998118644
1116	2	-0.9293788142405859
1116	3	-0.7447988792349136
1116	4	-0.894138317905238
1116	5	-1.029023316035051
1116	6	-1.7005351682497611
1116	7	-1.0960765952319136
1116	8	-1.3066059021043301
1116	9	-1.4479122229301211
1116	10	-1.3826647819291722
1116	11	-1.1967999331308334
1116	12	-1.8803351363160914
1116	13	-1.4386346450248837
1116	14	-1.9225268650001297
1116	15	-1.6759304730315776
1116	16	-1.8436198415749345
1116	17	-1.7181545293600138
1116	18	-2.6070930891498634
1116	19	-1.9700412863516235
1116	20	-1.770553498257982
1116	21	-1.900731664197309
1116	22	-3.12882705745276
1116	23	-2.9936685824841476
1116	24	-2.241905558598795
1116	25	-2.59619765763572
1201	1	0.6671961233809185
1201	2	0.6131369565060432
1201	3	0.5064556215358778
1201	4	0.5902643391289075
1201	5	0.7466414860710486
1201	6	0.7445910882620481
1201	7	0.6827446112550248
1201	8	0.6531462060527033
1201	9	0.9355279024213417
1201	10	0.8082151548161889
1201	11	0.9337695235509358
1201	12	0.7665983148455737
1201	13	0.7045550439733645
1201	14	0.7984013481054362
1201	15	0.9602705973994503
1201	16	0.8375312813988884
1201	17	0.7618070821927603
1201	18	0.8837251667822272
1201	19	0.6933963429564471
1201	20	0.8718439258360107
1201	21	0.8743880203426571
1201	22	0.9660702503544201
1201	23	0.9299328338521846
1201	24	0.9321850532960099
1201	25	0.8788919572553411
1202	1	0.6311637044355898
1202	2	0.661181488955183
1202	3	0.5875904714315183
1202	4	0.6581821565078485
1202	5	0.8630489693033212
1202	6	0.8789147097299548
1202	7	0.7057123683523656
1202	8	0.6905528130645067
1202	9	0.9172979675956299
1202	10	0.9676062111784347
1202	11	0.883727274023677
1202	12	0.928199922373949
1202	13	0.8455307823846496
1202	14	0.9473379370231285
1202	15	1.0402979257241611
1202	16	0.9376451565486335
1202	17	1.1173367649188108
1202	18	1.0348213265066164
1202	19	1.190321799474873
1202	20	1.3458234434249121
1202	21	1.2422983011845403
1202	22	1.2848095110565012
1202	23	1.2713769641197672
1202	24	1.0565074690605911
1202	25	1.1449447633533651
1203	1	0.5442071826964572
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2211	9	-0.14187478308392087
2211	10	-0.20126332896699495
2211	11	0.008634176961066942
2211	12	-0.05689535455629624
2211	13	-0.03525873363598464
2211	14	-0.8899364066312643
2211	15	-0.3006302382353141
2211	16	-0.10313139169713281
2211	17	0.02614746953142344
2211	18	-0.16229651240185916
2211	19	-0.1236844420502834
2211	20	-0.1779772289214776
2211	21	-0.2190743746969943
2211	22	-0.14953482584935784
2211	23	-0.05970195892811603
2211	24	-0.08091737599818316
2211	25	-0.0668506884537976
2212	1	-0.3312042714548653
2212	2	-0.23015663655092666
2212	3	-0.2341133102683557
2212	4	-0.05562813315027171
2212	5	-0.2299426573156751
2212	6	-0.2435331713780755
2212	7	-0.28712082079683654
2212	8	-0.47672163553792046
2212	9	-0.5570591721145206
2212	10	-0.5140844761205088
2212	11	-0.4445843986750404
2212	12	-0.29439765294191034
2212	13	-0.3641722575040873
2212	14	-0.5273484411454916
2212	15	-0.5189969301981492
2212	16	-0.39698809947286406
2212	17	-0.5602016243195962
2212	18	-0.32064876214527516
2212	19	-0.39982836946475686
2212	20	-0.5824559884952976
2212	21	-0.4874402308832515
2212	22	-0.5040842921224282
2212	23	-0.9112917731516958
2212	24	-0.6541818859155057
2212	25	-0.841247508491044
2213	1	-0.30513369200023277
2213	2	-0.3118755899115726
2213	3	-0.22829074261828453
2213	4	-0.3435919520151529
2213	5	-0.17203556679954346
2213	6	-0.04744567363626828
2213	7	-0.5927389316716187
2213	8	-0.18561718654267167
2213	9	-0.393391886121627
2213	10	-0.3443446722566037
2213	11	-0.3322899598202085
2213	12	-0.44337358888713396
2213	13	-0.2968584410787116
2213	14	-0.2721783418115393
2213	15	-0.36133049341624
2213	16	-0.5869747283557984
2213	17	-0.4917668230204626
2213	18	-0.642127404620858
2213	19	-0.9979908957883197
2213	20	-0.5137528417993735
2213	21	-1.3335072895407833
2213	22	-0.7341309327940948
2213	23	-0.7645963774839899
2213	24	-0.8022022617045508
2213	25	-0.8446607037001215
2214	1	-0.8553880967654521
2214	2	-0.8899669974880489
2214	3	-0.7432118326063168
2214	4	-1.331748491664996
2214	5	-1.086496278798876
2214	6	-2.5263801797180427
2214	7	-1.1484496784637201
2214	8	-1.6225879884465293
2214	9	-1.240042872844704
2214	10	-1.48647538175085
2214	11	-1.2460634449428198
2214	12	-1.265280789599693
2214	13	-1.4696084619543797
2214	14	-1.6903788595280247
2214	15	-2.0847605042715855
2214	16	-1.4155228954724492
2214	17	-1.5876899449065078
2214	18	-1.4789721175108852
2214	19	-3.0800467312324145
2214	20	-1.7780181575785932
2214	21	-2.31811607958727
2214	22	-1.9821761355663838
2214	23	-1.9877035113944217
2214	24	-1.4211417779458202
2214	25	-1.1817063796541376
2215	1	-1.4398022734751414
2215	2	-1.2157275337499023
2215	3	-1.1998915730327582
2215	4	-1.0638065391583993
2215	5	-1.795833082621165
2215	6	-1.5680281865639394
2215	7	-1.6530007730939715
2215	8	-2.37470237195933
2215	9	-1.9496257010554956
2215	10	-1.4651589729938124
2215	11	-2.194786257465104
2215	12	-2.8189433439746487
2215	13	-2.2168373002913953
2215	14	-2.1850155278586243
2215	15	-3.3414081545603977
2215	16	-2.7504298340733158
2215	17	-2.65850432128817
2215	18	-3.2815942383124295
2215	19	-3.7618245248571753
2215	20	-3.2858239052788036
2215	21	-2.445780630681895
2215	22	-3.2950207479888327
2215	23	-3.294518743513084
2215	24	-2.3326186373910147
2215	25	-2.4425735264843453
2216	1	-1.0421664176395176
2216	2	-0.9286429606852877
2216	3	-1.2988530056942977
2216	4	-0.7594080661330835
2216	5	-0.8292953012255566
2216	6	-1.2431081843183875
2216	7	-2.4180859636851153
2216	8	-1.6509591787858042
2216	9	-1.627954028681387
2216	10	-1.664970206362696
2216	11	-2.0614285973292468
2216	12	-1.614284527380022
2216	13	-1.9660618834609949
2216	14	-2.0237428618281967
2216	15	-2.0616567089869378
2216	16	-1.83250823187268
2216	17	-2.0884676907374455
2216	18	-1.7777199610991588
2216	19	-2.09395414928877
2216	20	-2.3395578355544444
2216	21	-2.1974332158724863
2216	22	-2.472722426538205
2216	23	-1.5693222578004011
2216	24	-1.7207866888141368
2216	25	-1.6881224233574201
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69229.0
3	2.0
4	1065.0
5	9558.0
6	11513.0
7	9052.0
8	7367.0
9	6613.0
10	5487.0
11	5081.0
12	4467.0
13	4163.0
14	4016.0
15	4131.0
16	4055.0
17	4303.0
18	4778.0
19	5680.0
20	7130.0
21	9641.0
22	11987.0
23	14786.0
24	16907.0
25	19696.0
26	22373.0
27	24344.0
28	27621.0
29	33042.0
30	42023.0
31	53446.0
32	67864.0
33	87353.0
34	114273.0
35	149268.0
36	204805.0
37	322737.0
38	1062063.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.40285007085992	19.59313507794591	13.824092699142499	27.179922152051674
2	13.75771946696423	20.405168525211693	39.67889627630288	26.1582157315212
3	16.685910097356395	24.400846846898286	30.470541645135913	28.442701410609406
4	11.46469659713408	17.170423988436124	37.58562038545442	33.779259028975375
5	12.871608811304865	37.957762259340754	35.862405912842874	13.308223016511503
6	32.03190945707881	37.70590367406116	17.42562650718469	12.836560361675344
7	27.456268515983574	31.948984117412095	22.237132704699412	18.357614661904922
8	24.927776040829087	37.14727810163027	20.203280139166324	17.721665718374314
9	25.712315266915457	15.155672629407526	20.13838020481576	38.99363189886125
10	14.430235331011817	29.166639444404517	34.62075577710848	21.78236944747519
11	33.94614331057695	22.75470008149228	24.56902493957417	18.730131668356606
12	22.678024606149457	25.06888636798435	31.61940378568069	20.633685240185503
13	28.432833294517412	22.0533310383674	26.038006430527176	23.475829236588012
14	20.79855805822423	21.940829483614184	27.70072447866659	29.55988797949499
15	22.697706935237104	30.802093407137992	23.635914225942276	22.864285431682628
16	22.206598280305755	28.282516768201894	27.26610387840902	22.244781073083335
17	21.455026433439873	28.627101232037376	27.936298633000717	21.981573701522034
18	21.45205772209638	27.74125713838245	29.410543557082182	21.396141582438986
19	22.98757938519731	27.178481538748294	28.018228158695756	21.81571091735864
20	23.14152970048875	27.32223899474704	28.24251629746945	21.293715007294757
21	22.631525712952648	26.770492953265933	27.762044050448708	22.835937283332708
22	22.798261766823074	27.62020706264287	27.759445168368398	21.822086002165655
23	22.08282573771809	27.453924864565266	28.068178434931983	22.39507096278466
24	22.68301794758226	26.820717495446424	28.487045651608252	22.009218905363067
25	22.377399338124874	27.17844117558289	28.312128694497396	22.132030791794836
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	249.0
2	549.0
3	849.0
4	849.0
5	849.0
6	2160.0
7	3471.0
8	3471.0
9	3471.0
10	4992.0
11	6513.0
12	6513.0
13	6513.0
14	10478.0
15	14443.0
16	14443.0
17	14443.0
18	24227.5
19	34012.0
20	34012.0
21	34012.0
22	51109.5
23	68207.0
24	68207.0
25	68207.0
26	94514.0
27	120821.0
28	120821.0
29	120821.0
30	151135.0
31	181449.0
32	181449.0
33	181449.0
34	204297.0
35	227145.0
36	227145.0
37	227145.0
38	245805.0
39	264465.0
40	264465.0
41	264465.0
42	279841.0
43	295217.0
44	295217.0
45	295217.0
46	302412.0
47	309607.0
48	309607.0
49	309607.0
50	301594.0
51	293581.0
52	293581.0
53	293581.0
54	266348.5
55	239116.0
56	239116.0
57	239116.0
58	206804.5
59	174493.0
60	174493.0
61	174493.0
62	143228.5
63	111964.0
64	111964.0
65	111964.0
66	86579.0
67	61194.0
68	61194.0
69	61194.0
70	44866.0
71	28538.0
72	28538.0
73	28538.0
74	19707.5
75	10877.0
76	10877.0
77	10877.0
78	7461.0
79	4045.0
80	4045.0
81	4045.0
82	2654.0
83	1263.0
84	1263.0
85	1263.0
86	794.0
87	325.0
88	325.0
89	325.0
90	195.5
91	66.0
92	66.0
93	66.0
94	37.0
95	8.0
96	8.0
97	8.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08218052880213417
2	4.0784381539520675E-5
3	0.0
4	0.03417731173011833
5	0.07871385637127491
6	0.08103856611902759
7	0.04062124401336259
8	0.0775311093066288
9	0.05807695931227745
10	0.12002843487080934
11	0.006240010375546663
12	0.017047871483519644
13	0.00717805115095564
14	0.009176485846392152
15	0.00905413270177359
16	0.022553762991354934
17	0.03495221497936922
18	0.0013458845908041824
19	0.004404713206268233
20	0.006443932283244268
21	1.22353144618562E-4
22	1.631375261580827E-4
23	0.0
24	4.0784381539520675E-5
25	0.003956085009333505
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2451919.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.94157305700257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69690604717923	52.68094685517316
2	13.658985024386805	18.28707887788987
3	4.0294641813686605	8.092160126329958
4	1.5212080857172818	4.073282488197858
5	0.735294697068411	2.46108918411158
6	0.38980779923844183	1.5656608362545728
7	0.21963161996817324	1.0291740293609104
8	0.15371370360177328	0.8231869695616435
9	0.10686099924500417	0.6438099049113368
>10	0.45144946685774606	5.115035315288319
>50	0.02101890585188461	0.9767702351992843
>100	0.012667705870527941	1.5655262756088053
>500	0.0020759176319947643	0.9091593314363581
>1k	7.326768112749372E-4	0.9767618100280815
>5k	1.831692028187343E-4	0.800357760648167
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8338	0.3400601732765234	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6006	0.2449509955263612	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5238	0.2136285905040093	No Hit
GTCCTACAGTGGACATTTCTAAATT	2892	0.11794843141229379	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2803	0.11431862145527646	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2683	0.10942449567053399	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2681	0.10934292690745495	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2584	0.10538684189812142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.0784381539520675E-5	0.0	0.0	0.0
6	0.0	4.0784381539520675E-5	0.0	0.0	0.0
7	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
8	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
9	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
10	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
11	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
12	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
13	0.0	4.0784381539520675E-5	0.0	4.0784381539520675E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCGT	75	9.547784E-7	13.950242	6
GGTATCA	1380	0.0	12.537271	1
TAGGACC	630	0.0	12.535673	4
TAGCACC	185	5.456968E-12	10.800843	4
CTAGCAC	275	0.0	10.717267	3
TCGAACT	190	9.094947E-12	10.488516	19
GTAGGAC	1850	0.0	10.380893	3
TGGCGAG	1695	0.0	10.301195	18
CTGTAGG	1930	0.0	10.294368	1
TGTAGGA	1970	0.0	10.279416	2
GTATCAA	2800	0.0	10.219253	1
GTATTAA	355	0.0	10.175738	1
GGCGAGG	795	0.0	10.146124	19
AGGACGT	1735	0.0	10.145708	5
GGACGTG	1725	0.0	10.14563	6
TTAGGAC	1175	0.0	9.952272	3
TAGGACG	1860	0.0	9.924277	4
GACCGTG	155	4.041067E-8	9.81033	7
TAGGACT	440	0.0	9.731279	4
TATGGCG	1810	0.0	9.650045	16
>>END_MODULE