FastQCFastQC Report
Thu 26 May 2016
SRR1512924_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512924_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756093
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT49320.1789489687031606No Hit
GTATCAACGCAGAGTACTTTTTTTT48650.17651799122888814No Hit
CTGTAGGACGTGGAATATGGCAAGA43570.15808610231947906No Hit
GTCCTAAAGTGTGTATTTCTCATTT41870.15191795051908624No Hit
CTTTAGGACGTGAAATATGGCGAGG38490.13965421341006998No Hit
TATCAACGCAGAGTACTTTTTTTTT30040.10899487063752927No Hit
GTCCTACAGTGTGCATTTCTCATTT29440.10681787588444948No Hit
GGTATCAACGCAGAGTACTTTTTTT29090.10554796227848626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9150.011.7277914
GGCGAGG10300.011.523519
ACGAGAG855.3102496E-511.17973915
TGCGTGA700.001497444610.85243410
GTCCTAC23300.010.7953291
GGTATCA13400.010.6653311
AGGACGT23600.010.542615
GGACGTG23500.010.5049366
AATGTCC6950.010.2464238
TGTAGGA26950.010.2189442
TCCAACG2053.6379788E-1210.19773318
TGGCGAG24950.010.13085118
GACGTGG12500.010.1025177
TCCTACA26850.010.0447892
GTATCAA25300.09.9796021
AGGACCT17400.09.8784365
GTAGGAC25750.09.8467493
GGACCTG16500.09.8401146
GCCGGTT1451.6286867E-79.82332411
TACACGG1554.090907E-89.8027325
Produced by FastQC (version 0.11.5)