##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512924_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2756093
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.2401040168093	31.0	30.0	34.0	25.0	34.0
2	29.356827581652723	31.0	30.0	34.0	23.0	34.0
3	29.5727426469281	31.0	30.0	34.0	25.0	34.0
4	33.01985636914284	35.0	35.0	37.0	26.0	37.0
5	33.171473894386004	37.0	35.0	37.0	27.0	37.0
6	33.18770593009742	37.0	35.0	37.0	27.0	37.0
7	33.046954511331805	37.0	35.0	37.0	26.0	37.0
8	32.996688428148104	37.0	35.0	37.0	26.0	37.0
9	34.353230460655716	39.0	35.0	39.0	26.0	39.0
10	34.25269285180144	39.0	35.0	39.0	25.0	39.0
11	34.296462419809494	39.0	35.0	39.0	26.0	39.0
12	34.2030584599286	39.0	35.0	39.0	25.0	39.0
13	34.19491504822225	39.0	35.0	39.0	25.0	39.0
14	35.21801659087701	40.0	35.0	41.0	25.0	41.0
15	35.163736855033555	40.0	35.0	41.0	25.0	41.0
16	35.05687725341634	39.0	35.0	41.0	24.0	41.0
17	35.00570118642586	39.0	35.0	41.0	24.0	41.0
18	34.71539022812365	39.0	34.0	41.0	19.0	41.0
19	34.95068199803127	39.0	34.0	41.0	23.0	41.0
20	35.03495709324758	40.0	35.0	41.0	24.0	41.0
21	34.97006958763728	40.0	35.0	41.0	24.0	41.0
22	34.892539547830935	40.0	35.0	41.0	22.0	41.0
23	34.63339154375415	39.0	34.0	41.0	18.0	41.0
24	34.63643353108912	39.0	35.0	41.0	18.0	41.0
25	34.557086063496406	39.0	35.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.49091302594661457
1101	2	0.7928053289781118
1101	3	0.7879621185173384
1101	4	0.8148553133061469
1101	5	0.643523256136433
1101	6	0.7385969095624461
1101	7	0.8186532979888952
1101	8	0.5919409585580624
1101	9	0.4615973344139732
1101	10	0.6211438393435742
1101	11	0.7789920397691574
1101	12	0.9960959366626838
1101	13	0.7793017872965322
1101	14	0.7883166761735794
1101	15	0.5725954342433397
1101	16	1.0477434775116308
1101	17	1.0306624274208644
1101	18	1.2739727112716466
1101	19	0.7471401185459285
1101	20	0.8506369488085639
1101	21	0.9270553412276641
1101	22	0.9066004062957091
1101	23	1.0936903830107028
1101	24	0.794606986839554
1101	25	1.0189538860912748
1102	1	0.5311433033578901
1102	2	0.5910074152401883
1102	3	0.7138170833167159
1102	4	0.7497907985703662
1102	5	0.6932529783350461
1102	6	0.6250718177120262
1102	7	0.798630420719455
1102	8	0.7088317676638312
1102	9	0.7049379730548182
1102	10	0.8684542554674479
1102	11	0.9279820821659541
1102	12	0.8536720885722104
1102	13	0.8497791587233507
1102	14	0.905152813899754
1102	15	1.0871408913219867
1102	16	0.9995293953122868
1102	17	0.8923883655352043
1102	18	1.2122354599156608
1102	19	1.2614365728478205
1102	20	1.1277306717706779
1102	21	0.920126287234801
1102	22	1.1763346705100588
1102	23	1.4291240967494687
1102	24	1.3097456367462783
1102	25	1.2213633836412328
1103	1	0.3253986723722946
1103	2	0.5685062584252485
1103	3	0.5400504228597924
1103	4	0.6787029773322857
1103	5	0.2551788252683522
1103	6	0.6061565959941717
1103	7	0.4208452555940241
1103	8	0.5375220346299514
1103	9	0.7435605683957647
1103	10	0.7109015241003647
1103	11	0.7857697275463096
1103	12	0.8619966217352442
1103	13	0.8018284557021929
1103	14	0.9824662389078256
1103	15	1.0891250406355155
1103	16	1.0651866055062484
1103	17	0.897027698772753
1103	18	1.281884132351017
1103	19	1.1860923282138245
1103	20	1.0399371863186886
1103	21	0.8388740097980758
1103	22	1.0454922087641663
1103	23	1.3473910104218447
1103	24	1.1483032209998285
1103	25	1.07352904500339
1104	1	0.46496855621516175
1104	2	0.5071157522826191
1104	3	0.5258683542933973
1104	4	0.7850874480353198
1104	5	0.44399662891749614
1104	6	0.43347173887514856
1104	7	0.620601409049435
1104	8	0.6418908902831433
1104	9	0.7909828601308604
1104	10	0.7946895835402046
1104	11	0.8146042382215981
1104	12	0.7055269789931344
1104	13	0.7199299165637143
1104	14	0.9705105769473619
1104	15	1.0562549699738781
1104	16	0.8525773418721201
1104	17	0.9109719403930967
1104	18	1.1338179547855347
1104	19	0.9743681567798816
1104	20	0.9675327157635039
1104	21	1.008703042638679
1104	22	0.9027405974654812
1104	23	1.2617832369023745
1104	24	1.1255764907084682
1104	25	0.8701145597371536
1105	1	0.5691118208003161
1105	2	0.7176611999948861
1105	3	0.6727235321213776
1105	4	0.7674990351453488
1105	5	0.5283365127457387
1105	6	0.4714884463954121
1105	7	0.6597498725505417
1105	8	0.6971928449920242
1105	9	0.7059586611606221
1105	10	0.8620653419764395
1105	11	0.8027065503820481
1105	12	0.7991297024734436
1105	13	0.732761425752642
1105	14	0.99708872980802
1105	15	1.0215640027249577
1105	16	1.122782020478894
1105	17	0.9291638659964008
1105	18	0.9746425988998979
1105	19	0.919682868668076
1105	20	1.0314365796646499
1105	21	0.9712719961323941
1105	22	0.6937541981636812
1105	23	0.9935573826307049
1105	24	1.0440353566349003
1105	25	1.0123282957324449
1106	1	0.6108940065045196
1106	2	0.6747347457152237
1106	3	0.7004367592612084
1106	4	0.5639078633202317
1106	5	0.6577892465562698
1106	6	0.5142569546455746
1106	7	0.5604707113447702
1106	8	0.600258090144429
1106	9	0.734271327087967
1106	10	0.8367179447253719
1106	11	0.7052614114231446
1106	12	0.8074096870148963
1106	13	0.7496687920866165
1106	14	0.8953038556607567
1106	15	0.9754765275090449
1106	16	0.9436972673115704
1106	17	0.9094113707851932
1106	18	1.0810268110180559
1106	19	0.6637863569229339
1106	20	0.7772190846214642
1106	21	0.7674216415939696
1106	22	0.6958973913619246
1106	23	0.9704796194153218
1106	24	0.8033432505758213
1106	25	0.9742229759757493
1107	1	0.3507438453705731
1107	2	0.43662709752308615
1107	3	0.5811074465915702
1107	4	0.6247221671459897
1107	5	0.5963799331056805
1107	6	0.44727176908190813
1107	7	0.6797964411830932
1107	8	0.548600776225264
1107	9	0.7197887959349885
1107	10	0.5458044515126019
1107	11	0.48859399703516004
1107	12	0.7623918268603873
1107	13	0.7954093456662434
1107	14	0.9724170303442463
1107	15	1.0705491894052486
1107	16	0.9362071766960582
1107	17	1.0574053591057861
1107	18	1.375289732265557
1107	19	1.207870603930182
1107	20	1.0551188517478138
1107	21	1.0995465741951946
1107	22	1.0504312203514132
1107	23	1.1005153148003046
1107	24	1.139357060988587
1107	25	1.0755581154714662
1108	1	0.4438969270074402
1108	2	0.4838712173516946
1108	3	0.38680320812765956
1108	4	0.5310435429559135
1108	5	0.4953399841070052
1108	6	0.6505714284600472
1108	7	0.773147674021736
1108	8	0.7329788603652005
1108	9	0.8435030863197213
1108	10	0.8469359924434201
1108	11	0.7794174355791696
1108	12	0.880812072917287
1108	13	0.7822014621192821
1108	14	0.9842591571160568
1108	15	1.1096043366368562
1108	16	0.969759043373351
1108	17	0.8792113905852119
1108	18	1.0698818748731185
1108	19	1.065953221589801
1108	20	0.9863419437443284
1108	21	0.9363207604930892
1108	22	0.971144891609427
1108	23	1.0928734418091466
1108	24	1.0421364393690098
1108	25	1.1732651500179188
1109	1	0.2529643619988988
1109	2	0.23113475398894678
1109	3	0.4683880784188936
1109	4	0.538187391116189
1109	5	0.3300801657671073
1109	6	0.2898975101885881
1109	7	0.42423818958863535
1109	8	0.4095915099757903
1109	9	0.5711310222638772
1109	10	0.7447797807902887
1109	11	0.25357557729378044
1109	12	0.5076581181411512
1109	13	0.4764182148062659
1109	14	0.4398016092190886
1109	15	0.7220507666397538
1109	16	0.5807853674386863
1109	17	0.4603236116358289
1109	18	-0.9804742307191248
1109	19	0.3380211313887145
1109	20	0.48883714942228096
1109	21	0.4464424838591654
1109	22	0.6154206869119108
1109	23	0.39606467205376816
1109	24	0.6262024869360872
1109	25	0.6194976761785753
1110	1	0.3694485155662406
1110	2	0.2923284347133297
1110	3	0.4106604287387121
1110	4	0.1997083980725236
1110	5	0.491652708012154
1110	6	0.36141676432792025
1110	7	0.3523359696212154
1110	8	0.34340461524541865
1110	9	0.6326634369318285
1110	10	0.8118363978082073
1110	11	0.6802801558287257
1110	12	0.22487051368447197
1110	13	0.4769201438307533
1110	14	0.6613887438714343
1110	15	0.7247998296952858
1110	16	0.8446483601021626
1110	17	0.3197974229656566
1110	18	0.8187632630241239
1110	19	0.7032307380602845
1110	20	0.71606030051408
1110	21	0.7229684993049332
1110	22	0.7129007141587564
1110	23	0.62709325675484
1110	24	0.8572094700191357
1110	25	0.8438841233645746
1111	1	-1.1233649334840834E-4
1111	2	0.3130092536931208
1111	3	0.25729997608205224
1111	4	0.24796215648469655
1111	5	0.2566101525805635
1111	6	0.3263896195324705
1111	7	0.261708268514532
1111	8	0.0855530447190418
1111	9	0.3718305663421191
1111	10	0.30063503046449114
1111	11	0.21795258089397862
1111	12	0.3489560643663623
1111	13	0.3297672482443943
1111	14	0.1673685376830676
1111	15	0.46797202636047786
1111	16	0.30978551051845926
1111	17	0.33615647125910897
1111	18	0.4853282534550942
1111	19	0.18206127879955147
1111	20	0.16300631998497295
1111	21	0.40226467050479187
1111	22	0.1709056693363067
1111	23	0.3363660869967049
1111	24	0.389563527474067
1111	25	0.018028981646160958
1112	1	-0.2686729082740662
1112	2	-0.005137070111970843
1112	3	0.09253227815878873
1112	4	0.16981990513044565
1112	5	0.033613841488417506
1112	6	0.02400928279034531
1112	7	-0.2706996057523199
1112	8	0.013036377836904478
1112	9	0.20769485245656938
1112	10	0.3128898753881444
1112	11	0.22266067882241458
1112	12	0.20306762366253395
1112	13	-0.05234973667676712
1112	14	0.22935522572471712
1112	15	0.14933571561308412
1112	16	0.29382258059477806
1112	17	0.3277682998972722
1112	18	0.24291669458986576
1112	19	0.14132017893616222
1112	20	0.013009228878431145
1112	21	-0.010712657364130962
1112	22	0.20386903451663585
1112	23	0.35236640158814936
1112	24	0.034726684075955916
1112	25	0.27078251864512737
1113	1	-0.26536944523361683
1113	2	-0.1417305927437873
1113	3	-0.2769534138568126
1113	4	-0.016209434993264438
1113	5	-0.21725448608239617
1113	6	-0.12857463359740962
1113	7	-0.15046961415251303
1113	8	0.0026348800212190326
1113	9	-0.16478489095559468
1113	10	-0.3268080335157961
1113	11	-0.31738535109703037
1113	12	-0.20232324882563546
1113	13	-0.23492472620943516
1113	14	-0.1462960528695305
1113	15	-0.5787912511258213
1113	16	-0.536520826830575
1113	17	-0.5346685998770582
1113	18	-0.6311195868878343
1113	19	-0.5098528203554835
1113	20	-0.5943882110364598
1113	21	-0.4402563344384376
1113	22	-0.39998636623069217
1113	23	-0.7597206655317947
1113	24	-0.2942803283768711
1113	25	-0.2762027586271998
1114	1	-1.1490357689196848
1114	2	-0.8997325117255741
1114	3	-0.7533057030118933
1114	4	-0.9270551546274604
1114	5	-0.8785627487093777
1114	6	-1.3649283331399964
1114	7	-1.539708357497247
1114	8	-1.058106235873101
1114	9	-1.0925216570866354
1114	10	-1.2981799452212641
1114	11	-1.9874136015861268
1114	12	-1.4847805391934372
1114	13	-1.4498137703834146
1114	14	-1.6604376985139453
1114	15	-1.5806840018409005
1114	16	-2.2455851308844785
1114	17	-4.257857293697956
1114	18	-3.3065692280403773
1114	19	-2.182472711306886
1114	20	-1.3931820091779556
1114	21	-2.3875443302243724
1114	22	-2.408346438160457
1114	23	-2.3397239884902348
1114	24	-1.8831396026897593
1114	25	-1.6750240165860077
1115	1	-0.9230166107494426
1115	2	-0.5208195498210522
1115	3	-0.7359193221873745
1115	4	-0.6456234051069956
1115	5	-0.5133804855216795
1115	6	-0.6668147467589023
1115	7	-1.5711795941402258
1115	8	-1.00248940463214
1115	9	-0.8100974868627802
1115	10	-0.5695164883891692
1115	11	-1.2826252414082404
1115	12	-0.8873509723961703
1115	13	-0.7934450998679807
1115	14	-0.9311887391223195
1115	15	-2.093663106683451
1115	16	-0.9123586581821215
1115	17	-0.8610445068307016
1115	18	-0.9166318854032554
1115	19	-0.9639764077031145
1115	20	-0.8324642002179914
1115	21	-1.5055170167363272
1115	22	-1.48961829829841
1115	23	-0.9829224766465501
1115	24	-0.7514374444619634
1115	25	-1.066962932461486
1116	1	-1.09139969036465
1116	2	-1.848720572551418
1116	3	-1.4265148268884893
1116	4	-1.014507196462752
1116	5	-1.5555651663264456
1116	6	-1.3226607659985632
1116	7	-1.0374740325905378
1116	8	-1.751117264061115
1116	9	-1.653486119867594
1116	10	-2.5879909155966985
1116	11	-1.2598864909889045
1116	12	-1.2448572284595656
1116	13	-1.7091656125025096
1116	14	-1.5918393470484844
1116	15	-1.2002163330894788
1116	16	-1.067634718312867
1116	17	-1.4226796514001663
1116	18	-1.1942945657184154
1116	19	-1.7019351856854001
1116	20	-1.8812213742906891
1116	21	-2.395880072415295
1116	22	-1.603043447673258
1116	23	-1.4342598235316615
1116	24	-2.0842319101125497
1116	25	-1.847657423498994
1201	1	0.25293792883985233
1201	2	0.24589786174029982
1201	3	0.4558090709985514
1201	4	0.44160595765943356
1201	5	0.47044233988420814
1201	6	0.4941652110108805
1201	7	0.3924500816677039
1201	8	-0.07781473171673525
1201	9	0.18591068977027447
1201	10	0.48191924138372144
1201	11	0.05819630883657112
1201	12	-0.4268132730516214
1201	13	-0.12731018496904767
1201	14	0.43629213629628083
1201	15	0.032413200243262
1201	16	0.484259976585939
1201	17	0.7328456052623693
1201	18	0.9803138736486758
1201	19	0.6705154407225749
1201	20	0.5395620586208807
1201	21	0.4495942746868451
1201	22	0.695344008139827
1201	23	0.9851232069116733
1201	24	0.6786466281798127
1201	25	0.6661205467076741
1202	1	0.5849475179883932
1202	2	0.6146593174294885
1202	3	0.39386235641243417
1202	4	0.5959253467652914
1202	5	0.5708337229712228
1202	6	0.6705869871942696
1202	7	0.5874968421313511
1202	8	0.7018751940247654
1202	9	1.0086853995521778
1202	10	1.0430456815196791
1202	11	0.7579438728799843
1202	12	0.8587663503144611
1202	13	0.8011147347233631
1202	14	0.9772131835659081
1202	15	0.6013029990876078
1202	16	0.8442477381963016
1202	17	1.0452996236650876
1202	18	1.3837743188491274
1202	19	1.0053174465389816
1202	20	0.8972079629668883
1202	21	0.9346469418276229
1202	22	1.0396572758599731
1202	23	1.1166362714911955
1202	24	1.2505651326862832
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2211	13	0.10727441584489128
2211	14	0.22277142886402856
2211	15	0.2632700285175673
2211	16	0.25650288576846236
2211	17	0.33540531710994514
2211	18	0.2513826727074786
2211	19	0.22290167590214338
2211	20	-0.0415316569411317
2211	21	0.012046417231836415
2211	22	-0.11487003441239096
2211	23	0.4928000224214628
2211	24	0.23210685430496625
2211	25	0.41156756338925504
2212	1	-0.05071669206122209
2212	2	-0.036826796738690604
2212	3	-0.08652202446187829
2212	4	-0.2137848685618735
2212	5	-0.15570697565929947
2212	6	-0.1289950590083464
2212	7	-0.2510318188226037
2212	8	-0.18558657155173108
2212	9	-0.060802581746166595
2212	10	0.0028587408048608154
2212	11	-0.2257952703147339
2212	12	-0.14003506850206549
2212	13	-0.2071380817842936
2212	14	-0.20040895786540602
2212	15	-0.3815253489069974
2212	16	-0.08222072931486935
2212	17	-0.20117989988781915
2212	18	-2.84284733935473
2212	19	-1.2284001917690617
2212	20	-0.5188179845763656
2212	21	-0.48531313498277484
2212	22	-0.13061272431713178
2212	23	-0.26970787247516625
2212	24	-0.5666338431751186
2212	25	-0.3043025402881909
2213	1	-0.07340955443350339
2213	2	0.08943877866445149
2213	3	-0.29638094802571757
2213	4	-0.3258484740321492
2213	5	-0.22124495306344016
2213	6	-0.18222889095216743
2213	7	-0.04111787595314098
2213	8	-0.4239877452128411
2213	9	-0.29714013732512257
2213	10	-0.3308304050579949
2213	11	-0.38310187614171554
2213	12	-0.45192653238554215
2213	13	-0.22492208702578864
2213	14	-0.1635726656810519
2213	15	-0.3673768217953608
2213	16	-0.923027807351481
2213	17	-0.32506324971118516
2213	18	-0.2787930439707367
2213	19	-0.14374334723081006
2213	20	-0.8431162230547358
2213	21	-0.5089028593313216
2213	22	-0.30170684232770384
2213	23	-0.8364778198899501
2213	24	-0.5697004206337866
2213	25	-0.8398668723075744
2214	1	-1.5194001505579315
2214	2	-3.5690852757267493
2214	3	-1.7976837178226823
2214	4	-1.299988098441581
2214	5	-1.317909851094047
2214	6	-1.357881658773529
2214	7	-1.423989218968817
2214	8	-1.1606467164311098
2214	9	-1.4565452547042312
2214	10	-1.5188330693012517
2214	11	-1.2063637114214885
2214	12	-1.7078036159423107
2214	13	-1.5710604583377616
2214	14	-2.587330603376138
2214	15	-2.853904782238118
2214	16	-2.011029794244756
2214	17	-2.4565521778612833
2214	18	-1.8615288040985334
2214	19	-4.239202788078401
2214	20	-2.253510835864553
2214	21	-2.1062654842882793
2214	22	-2.186794022914853
2214	23	-2.211779381746112
2214	24	-2.424342356500027
2214	25	-2.3336640963015682
2215	1	-0.6995605311696451
2215	2	-2.5342554463357168
2215	3	-1.1536486024176114
2215	4	-0.7012426792835811
2215	5	-0.6809273328145125
2215	6	-1.2557462119697433
2215	7	-1.7092650046526252
2215	8	-1.6782007952069833
2215	9	-1.334367199494487
2215	10	-2.7188668520977437
2215	11	-1.7702700664447804
2215	12	-1.9377269909488817
2215	13	-1.320413967832593
2215	14	-2.6237322095201634
2215	15	-2.487512586181005
2215	16	-2.0681989361734168
2215	17	-2.3598022816060293
2215	18	-1.2917822335199602
2215	19	-1.9597993642095162
2215	20	-2.1523964711623123
2215	21	-1.8395586055731954
2215	22	-1.4024773647231115
2215	23	-1.8987776809167443
2215	24	-1.9399341106921923
2215	25	-1.8463155197963914
2216	1	-1.7528448749750858
2216	2	-1.7070183432219181
2216	3	-2.29753448965036
2216	4	-2.832473359101609
2216	5	-1.9911980082484355
2216	6	-2.06156001090309
2216	7	-1.8771636202213529
2216	8	-2.3691030610747994
2216	9	-2.624192342308504
2216	10	-2.151134118791184
2216	11	-2.843036412451408
2216	12	-3.047852298575574
2216	13	-3.6174364154774707
2216	14	-2.971509726110199
2216	15	-2.9205324701799924
2216	16	-1.9803367394706797
2216	17	-2.2201669245403437
2216	18	-2.362596641757854
2216	19	-2.6402087327416623
2216	20	-2.4322986627598695
2216	21	-2.648348562792087
2216	22	-3.308579868255759
2216	23	-3.172849176496218
2216	24	-3.2254092156139578
2216	25	-3.169555428835757
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117808.0
3	3.0
4	1048.0
5	5724.0
6	7144.0
7	6607.0
8	5914.0
9	5695.0
10	5303.0
11	5108.0
12	4726.0
13	4673.0
14	4688.0
15	4761.0
16	4839.0
17	5328.0
18	5969.0
19	7230.0
20	9142.0
21	11691.0
22	14722.0
23	18352.0
24	21126.0
25	24428.0
26	27423.0
27	30605.0
28	34390.0
29	41306.0
30	50848.0
31	64586.0
32	81269.0
33	103797.0
34	134639.0
35	178108.0
36	243517.0
37	379537.0
38	1084039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.637368238999436	19.16401172422582	12.963607566130825	25.23501247064392
2	14.459524156071929	20.138450554488227	42.10963596839709	23.29238932104275
3	18.409615709879436	24.69676875155617	29.961657371373985	26.931958167190412
4	11.924456423505124	16.863048412843018	39.051270008689094	32.161225154962764
5	12.780125008166909	38.05406131352949	36.46554289987006	12.70027077843355
6	31.470976320323764	37.777197950945194	18.25224305889218	12.49958266983886
7	27.441631925493247	31.78759304634608	22.75944834113316	18.01132668702751
8	24.246329453504476	37.74714674346966	20.99818250970441	17.00834129332145
9	25.892159520499302	15.449243395016499	20.741976234724824	37.916620849759376
10	14.334141279390265	29.355695983410918	35.279674053826696	21.030488683372123
11	33.65724590004496	22.77307237426327	25.343639213392176	18.226042512299593
12	22.71376614101391	24.964417370274912	32.48216876581803	19.83964772289315
13	28.424877637902547	22.404412039552163	26.21508366203347	22.955626660511818
14	20.29823401937973	22.572969779189815	28.219650984828444	28.90914521660201
15	22.38255447643105	31.66169446743028	23.610578807543227	22.345172248595443
16	21.65956816736781	28.69067527196398	28.2236840195216	21.426072541146617
17	21.128260774892215	29.144214438137823	28.35977760680535	21.367747180164617
18	20.957063165093814	28.16632688973903	30.2078940840315	20.66871586113566
19	22.90553703843496	27.424395951525142	28.036719063020794	21.6333479470191
20	22.95113439565433	27.985219250684818	28.524436431309873	20.539209922350985
21	22.355564910956343	27.41071136935243	27.6579359001811	22.575787819510122
22	22.421101871969523	28.579116959355076	27.872288151862406	21.127493016812995
23	21.789579807047442	28.30887796607217	28.139144458928627	21.762397767951757
24	22.467727396112917	27.26728378843281	28.928671484997032	21.336317330457238
25	22.152845521374072	27.896401653717962	28.42690659512943	21.52384622977854
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	281.0
1	281.0
2	527.5
3	774.0
4	774.0
5	774.0
6	1706.0
7	2638.0
8	2638.0
9	2638.0
10	4248.5
11	5859.0
12	5859.0
13	5859.0
14	10193.5
15	14528.0
16	14528.0
17	14528.0
18	25967.0
19	37406.0
20	37406.0
21	37406.0
22	58421.0
23	79436.0
24	79436.0
25	79436.0
26	111810.0
27	144184.0
28	144184.0
29	144184.0
30	184145.5
31	224107.0
32	224107.0
33	224107.0
34	252666.0
35	281225.0
36	281225.0
37	281225.0
38	302153.5
39	323082.0
40	323082.0
41	323082.0
42	334670.0
43	346258.0
44	346258.0
45	346258.0
46	348979.0
47	351700.0
48	351700.0
49	351700.0
50	335440.0
51	319180.0
52	319180.0
53	319180.0
54	283132.0
55	247084.0
56	247084.0
57	247084.0
58	210443.0
59	173802.0
60	173802.0
61	173802.0
62	140186.0
63	106570.0
64	106570.0
65	106570.0
66	81695.5
67	56821.0
68	56821.0
69	56821.0
70	41365.5
71	25910.0
72	25910.0
73	25910.0
74	18025.5
75	10141.0
76	10141.0
77	10141.0
78	6877.5
79	3614.0
80	3614.0
81	3614.0
82	2335.5
83	1057.0
84	1057.0
85	1057.0
86	686.5
87	316.0
88	316.0
89	316.0
90	192.0
91	68.0
92	68.0
93	68.0
94	45.5
95	23.0
96	23.0
97	23.0
98	26.0
99	29.0
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34987934006581056
2	0.03737174326120345
3	0.03392483490216042
4	0.03319916998446714
5	0.03893192283424399
6	0.017452241270523167
7	0.01730710828698451
8	0.023402693595607985
9	0.020318617695411583
10	0.019810652253026296
11	0.018649588384717062
12	0.019411536548294996
13	0.019665519269487643
14	0.02684960195465102
15	0.028046949068844917
16	0.021080565858989517
17	0.02224162972729875
18	0.10217362041121253
19	0.024854023430994524
20	0.022386762710837407
21	0.02550712185691847
22	0.019810652253026296
23	0.021661097793144138
24	0.01832303917175509
25	0.021951363760221443
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2756093.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.44835755230588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76867275089981	56.09251664229675
2	12.742460693459599	17.698859326711716
3	3.550256740037154	7.3967849845375255
4	1.2821301767841953	3.561673397836398
5	0.5684090421423338	1.9737537197332247
6	0.3167488854005083	1.3198613908553318
7	0.18473035489119236	0.898045381607352
8	0.11694968229965928	0.6497570681580255
9	0.08058184025999647	0.5036648809139129
>10	0.35182561059626494	4.240692576543583
>50	0.021298239764727698	1.0230354721261468
>100	0.0129472453326208	1.825308500199991
>500	0.001677888074128076	0.8520277206125526
>1k	0.0013108500577927317	1.964018937867403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	4932	0.1789489687031606	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4865	0.17651799122888814	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4357	0.15808610231947906	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4187	0.15191795051908624	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3849	0.13965421341006998	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3004	0.10899487063752927	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2944	0.10681787588444948	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2909	0.10554796227848626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.6283245884663546E-5	0.0
4	0.0	0.0	0.0	3.6283245884663546E-5	0.0
5	0.0	0.0	0.0	3.6283245884663546E-5	0.0
6	0.0	0.0	0.0	3.6283245884663546E-5	0.0
7	0.0	0.0	0.0	3.6283245884663546E-5	0.0
8	0.0	0.0	0.0	3.6283245884663546E-5	0.0
9	3.6283245884663546E-5	0.0	0.0	1.8141622942331772E-4	0.0
10	7.256649176932709E-5	0.0	0.0	2.1769947530798125E-4	0.0
11	7.256649176932709E-5	0.0	0.0	2.9026596707730837E-4	0.0
12	1.8141622942331772E-4	0.0	0.0	3.265492129619719E-4	0.0
13	1.8141622942331772E-4	0.0	0.0	3.265492129619719E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	915	0.0	11.727791	4
GGCGAGG	1030	0.0	11.5235	19
ACGAGAG	85	5.3102496E-5	11.179739	15
TGCGTGA	70	0.0014974446	10.852434	10
GTCCTAC	2330	0.0	10.795329	1
GGTATCA	1340	0.0	10.665331	1
AGGACGT	2360	0.0	10.54261	5
GGACGTG	2350	0.0	10.504936	6
AATGTCC	695	0.0	10.246423	8
TGTAGGA	2695	0.0	10.218944	2
TCCAACG	205	3.6379788E-12	10.197733	18
TGGCGAG	2495	0.0	10.130851	18
GACGTGG	1250	0.0	10.102517	7
TCCTACA	2685	0.0	10.044789	2
GTATCAA	2530	0.0	9.979602	1
AGGACCT	1740	0.0	9.878436	5
GTAGGAC	2575	0.0	9.846749	3
GGACCTG	1650	0.0	9.840114	6
GCCGGTT	145	1.6286867E-7	9.823324	11
TACACGG	155	4.090907E-8	9.802732	5
>>END_MODULE