FastQCFastQC Report
Thu 26 May 2016
SRR1512924_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512924_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2756093
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60860.22081983445406234No Hit
GTCCTACAGTGGACATTTCTAAATT44400.16109761172790613No Hit
TATCAACGCAGAGTACTTTTTTTTT44170.16026309707255887No Hit
CTGTAGGACGTGGAATATGGCAAGA43840.15906574995836498No Hit
CTTTAGGACGTGAAATATGGCGAGG40550.14712856206231067No Hit
GTCCTAAAGTGTGTATTTCTCATTT39860.14462501809626888No Hit
GGTATCAACGCAGAGTACTTTTTTT36790.13348606160967716No Hit
GTCCTACAGTGTGCATTTCTCATTT28840.10464088113136966No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8850.012.1499334
GCGTGCG658.0151623E-411.6943439
GGTATCA13150.011.35160351
GGCGAGG10500.011.20654519
TAAGGCG600.00591459811.07399616
TAAACCG700.001471679210.8750355
AGGACCG909.36265E-510.5729515
TGGCGAG26000.010.54765518
ATTATAC3350.010.5016133
AGGACCT17350.010.4754485
TGTAGGA25450.010.4607162
ATAGGAC3750.010.3956513
GTCCTAC24150.010.3149591
TAGGACT5400.010.220524
GTAGGAC24950.010.06083
GTATCAA27200.09.9622931
CTGTAGG26400.09.9400481
TAGGACA9150.09.87964254
GACGTGA13900.09.8457657
GGACCTG17300.09.8424556
Produced by FastQC (version 0.11.5)