FastQCFastQC Report
Thu 26 May 2016
SRR1512923_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512923_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747461
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53080.30375499081238433No Hit
TATCAACGCAGAGTACTTTTTTTTT30000.17167765117504769No Hit
GGTATCAACGCAGAGTACTTTTTTT28790.16475331924432077No Hit
GTACTGGTTCACTATCGGTCAGTCA26010.14884452356876632No Hit
GTCCTACAGTGGACATTTCTAAATT17870.10226265421660341No Hit
GTCCTAAAGTGTGTATTTCTCATTT17550.10043142593740288No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG551.9628035E-413.816165
GGTATCA14550.012.954891
TGTACCG951.0418444E-611.9982455
TCCAACG1750.011.94179418
CGTTTCC1302.6193447E-911.69126814
GTCGATC658.0322125E-411.6909328
ATCCCGT1157.086237E-811.56385710
TCCCGTT1501.7644197E-1011.39865911
CCAACGA1354.7384674E-911.25761319
ACAACGC600.00588129711.0820298
TCTATAC1201.2779674E-711.0817133
TAGGACC3100.011.0306444
CCGCCCA700.00149302610.8558669
CGCCCTA3500.010.85586616
GTCCTAA5900.010.6493591
TTAGGAC7550.010.567993
CCCGTTT1451.4451871E-810.48182612
TTAGACT1002.4027126E-510.4484724
CCGTTTC1659.295036E-1010.36271413
GGCGAGG4400.010.36212119
Produced by FastQC (version 0.11.5)