##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512923_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1747461
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.88548757311322	31.0	30.0	34.0	22.0	34.0
2	28.89830674332646	31.0	30.0	34.0	18.0	34.0
3	29.065598030513986	31.0	30.0	34.0	22.0	34.0
4	32.49319727307219	35.0	33.0	37.0	23.0	37.0
5	32.664833721610954	35.0	33.0	37.0	25.0	37.0
6	32.55114305841447	36.0	33.0	37.0	24.0	37.0
7	32.45271224937209	36.0	33.0	37.0	23.0	37.0
8	32.380735249599276	36.0	33.0	37.0	22.0	37.0
9	33.67459416833909	38.0	34.0	39.0	22.0	39.0
10	33.55035219670138	38.0	34.0	39.0	19.0	39.0
11	33.64546104319353	38.0	34.0	39.0	21.0	39.0
12	33.54300954356063	38.0	34.0	39.0	18.0	39.0
13	33.51813116287001	38.0	34.0	39.0	18.0	39.0
14	34.35722285075318	39.0	34.0	41.0	17.0	41.0
15	34.53275008712641	39.0	34.0	41.0	18.0	41.0
16	34.47744870987106	39.0	34.0	41.0	17.0	41.0
17	34.21722315977295	39.0	33.0	41.0	17.0	41.0
18	34.21352293413129	39.0	33.0	41.0	17.0	41.0
19	34.33743986274944	39.0	34.0	41.0	18.0	41.0
20	34.305917556958356	39.0	34.0	41.0	18.0	41.0
21	34.20679660375825	39.0	34.0	41.0	15.0	41.0
22	34.20720634108572	39.0	34.0	41.0	11.0	41.0
23	33.865576971388776	39.0	34.0	41.0	8.0	41.0
24	33.929453075061474	39.0	34.0	41.0	2.0	41.0
25	33.64101401976925	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6725088572364619
1101	2	0.3272886117883971
1101	3	0.6783981801106442
1101	4	0.7818698149119854
1101	5	0.5774429017495137
1101	6	0.5981282657551787
1101	7	0.2942585792692256
1101	8	0.5592421958219305
1101	9	0.6576363307132951
1101	10	0.6250547295520477
1101	11	0.7167513516196209
1101	12	0.6411242897789364
1101	13	0.9281530635998507
1101	14	0.9865832367056484
1101	15	0.8839592121833277
1101	16	0.7283025813871049
1101	17	0.16174339268670934
1101	18	0.6070495754771486
1101	19	0.07897392464763442
1101	20	-0.769283828494892
1101	21	0.5017899684978033
1101	22	0.6246395381310634
1101	23	0.19157768178668277
1101	24	0.7544467830188708
1101	25	0.32612602815528646
1102	1	1.183006930094848
1102	2	1.3046045386813496
1102	3	1.192091080769604
1102	4	1.3784492007133053
1102	5	1.0746096305820387
1102	6	1.0684373544036916
1102	7	1.0591841045707326
1102	8	1.040410913961928
1102	9	1.3931738167259766
1102	10	1.470332362276153
1102	11	1.3297624171424332
1102	12	1.252605577855526
1102	13	1.2436990041692866
1102	14	1.522607108395313
1102	15	1.4201700787753992
1102	16	1.4708474029710956
1102	17	1.6624590460498823
1102	18	1.5764520372451472
1102	19	1.3498897520752067
1102	20	1.6112388620434643
1102	21	1.671172537191076
1102	22	1.414733781572565
1102	23	1.4004221428086936
1102	24	0.7718303463658813
1102	25	1.1572507328674604
1103	1	0.8694220350355444
1103	2	0.8641979544963974
1103	3	1.0659754678490074
1103	4	1.0593378025544737
1103	5	0.9542840650604774
1103	6	0.5846270406566347
1103	7	0.3688472657505315
1103	8	0.5782962066880231
1103	9	0.5918913642703387
1103	10	0.9751785177697982
1103	11	0.7127066088903149
1103	12	0.8615670297384526
1103	13	1.0935332702686509
1103	14	1.0148308131411454
1103	15	1.1200512543693293
1103	16	1.0081675009567235
1103	17	0.6058473662671915
1103	18	1.0467146441892012
1103	19	1.0576981176805518
1103	20	1.1448416642077817
1103	21	0.9042716985499979
1103	22	1.0964678112747706
1103	23	0.29146305517541293
1103	24	0.40170831492469716
1103	25	0.8933598621810646
1104	1	1.181885330511129
1104	2	1.0430354632401553
1104	3	1.1584274084748039
1104	4	1.3693370296104845
1104	5	1.0984403196764774
1104	6	1.0730932584656543
1104	7	1.257449670238998
1104	8	0.9626723129812973
1104	9	1.3031014727273131
1104	10	1.3319781280519862
1104	11	1.0319718114745342
1104	12	1.320860250854622
1104	13	1.2896838991727364
1104	14	1.703838861936191
1104	15	1.3336482743814244
1104	16	1.4486323841307822
1104	17	1.857598937117686
1104	18	1.5389490327148607
1104	19	1.1544862465812002
1104	20	1.2437292575215153
1104	21	1.3407256625836226
1104	22	1.1835718597133322
1104	23	1.5170402944230332
1104	24	1.2779097820879173
1104	25	0.8718728444767976
1105	1	0.5327660554645917
1105	2	0.6068928476687958
1105	3	0.7196393207529468
1105	4	0.8883217805819754
1105	5	0.6383324366332346
1105	6	0.757707861546983
1105	7	0.6738928539093152
1105	8	0.6433154239503907
1105	9	0.3169357493179419
1105	10	0.45936383807917736
1105	11	0.554689336115743
1105	12	0.7677427836881279
1105	13	0.7368460314846814
1105	14	0.77159777158937
1105	15	0.8063522077536476
1105	16	0.7374054329928654
1105	17	1.077753636319045
1105	18	0.9628423495635303
1105	19	0.5967228172279206
1105	20	0.8495065923246017
1105	21	0.7104924878776444
1105	22	0.5728578382418021
1105	23	0.8334863196604374
1105	24	0.8247455094861991
1105	25	1.1163250868485335
1106	1	0.6816764240407949
1106	2	0.7101279636892777
1106	3	0.5938274575295424
1106	4	0.8033694795665838
1106	5	0.7094784836620391
1106	6	0.6956251064484604
1106	7	0.6158120403868068
1106	8	0.7684843152048941
1106	9	0.7094554559912751
1106	10	0.6382180604144168
1106	11	0.37904243143182725
1106	12	0.5424456212766913
1106	13	0.5859315944339798
1106	14	0.9868204494558341
1106	15	0.6349151936863819
1106	16	0.7890729580329463
1106	17	0.7798968043849683
1106	18	0.8470796506651226
1106	19	0.8023784924050545
1106	20	0.9096399598492084
1106	21	0.7916036576502634
1106	22	0.7453421397054214
1106	23	1.1510223497947294
1106	24	0.8873440967256698
1106	25	0.8737665378803072
1107	1	0.9615326667602702
1107	2	0.9420028975026824
1107	3	0.9278743705868351
1107	4	1.0945364815786576
1107	5	0.9746571874637979
1107	6	0.9870330276599404
1107	7	0.771853817364466
1107	8	0.8712660053457384
1107	9	0.9536363307132945
1107	10	1.0273642533615686
1107	11	0.9817989706672421
1107	12	1.048290956445605
1107	13	0.8892006826474699
1107	14	1.1515832367056476
1107	15	1.0786258788499978
1107	16	1.1679930575775828
1107	17	1.430291011734326
1107	18	1.3486210040485815
1107	19	1.2201405913142978
1107	20	1.288954266743204
1107	21	1.0134804446882768
1107	22	1.100758585750114
1107	23	1.4503633960723974
1107	24	1.178113449685533
1107	25	1.575149837679099
1108	1	0.7203613917986722
1108	2	0.6889844643229601
1108	3	0.6299455431418792
1108	4	0.8109266505494688
1108	5	0.5431633318570377
1108	6	0.6572762432257377
1108	7	0.6399400948862279
1108	8	0.54123912362531
1108	9	0.704890811000034
1108	10	0.7576855539248086
1108	11	0.5807083408669342
1108	12	0.6714489185551784
1108	13	0.7536809693858189
1108	14	0.9759022331214169
1108	15	0.8552464625673508
1108	16	0.4787623868146582
1108	17	0.9277833312427788
1108	18	1.0182549006179613
1108	19	0.8958689579297641
1108	20	0.9479327613668573
1108	21	0.8782487498598073
1108	22	0.7590931991653704
1108	23	1.122328321827645
1108	24	0.8744344942323892
1108	25	0.9693190645096124
1109	1	0.5961117072031428
1109	2	0.5891152559013726
1109	3	0.6927513504422862
1109	4	0.8019089763246683
1109	5	0.6861648249644361
1109	6	0.8257384969305335
1109	7	0.649338183554427
1109	8	0.5859744732323549
1109	9	0.9259362948324181
1109	10	0.7935197456984397
1109	11	0.7050656680889418
1109	12	0.6931757532060701
1109	13	0.8393513823245442
1109	14	0.9590123719765486
1109	15	0.8900729033002506
1109	16	0.4851298918591951
1109	17	0.9684340482815585
1109	18	0.8900139508936249
1109	19	0.7812696855784864
1109	20	0.7863403449635129
1109	21	0.7232811776833046
1109	22	0.8218133553436289
1109	23	1.0688121889372297
1109	24	0.9402076113545093
1109	25	0.4665211278528645
1110	1	0.6769605309752649
1110	2	0.7572009173046652
1110	3	0.6833977086349243
1110	4	0.6256397347611156
1110	5	0.5644131987792136
1110	6	0.5482282186076048
1110	7	0.4428262360349038
1110	8	0.5840559629129274
1110	9	0.7479079007274407
1110	10	0.6639731642526598
1110	11	0.6654340484607317
1110	12	0.577035652343767
1110	13	0.6328640489841035
1110	14	0.5721702239758031
1110	15	0.6454009849320315
1110	16	0.7588947548901714
1110	17	0.7372888900935948
1110	18	0.054497948885924075
1110	19	0.46667878084753767
1110	20	0.6674761903641411
1110	21	0.7900822834435814
1110	22	0.7857974824969318
1110	23	0.7913145983354823
1110	24	0.6720809178609244
1110	25	0.9319921290510891
1111	1	0.47354199464169255
1111	2	0.407491014037312
1111	3	0.532485921826293
1111	4	0.43187611868573583
1111	5	0.5975788588157016
1111	6	0.5278163141408996
1111	7	0.3772135158055576
1111	8	0.5425048246659543
1111	9	0.7393250605880723
1111	10	0.6974172817285549
1111	11	0.28411688531073764
1111	12	-0.022261688583789407
1111	13	0.4516157094811035
1111	14	0.5581753655070827
1111	15	0.5279588714387984
1111	16	0.641346622617192
1111	17	0.14299290286645316
1111	18	0.5005491921395588
1111	19	0.42895671704903293
1111	20	0.6681224241671728
1111	21	0.6012628367148523
1111	22	0.2207502801022727
1111	23	0.5957627060647326
1111	24	0.6197477456221563
1111	25	0.731452546598085
1112	1	0.11784150761464929
1112	2	0.13844837207114224
1112	3	0.1633553417490532
1112	4	0.32806155456948716
1112	5	0.21371970347055935
1112	6	-0.05474666808353845
1112	7	0.017159226369230396
1112	8	0.13173481136940524
1112	9	0.11699237230526194
1112	10	0.11383111296306936
1112	11	0.0010004253617026393
1112	12	0.1465956483474642
1112	13	0.20482994044596126
1112	14	0.07138782616423356
1112	15	-0.05749869216880654
1112	16	0.20336454249281388
1112	17	0.5240894802074081
1112	18	0.22054873032016076
1112	19	0.23154624106075516
1112	20	0.02258818571057475
1112	21	0.023116130802094403
1112	22	-0.09930275215901929
1112	23	0.21302397590131505
1112	24	0.21318311111052424
1112	25	0.1054135010505064
1113	1	-0.1094884453882905
1113	2	-0.02053834121502618
1113	3	0.05422928292214024
1113	4	0.0027977119967488306
1113	5	-0.20210103434344262
1113	6	-0.3997130040860881
1113	7	-0.2659024332839408
1113	8	-0.17185854076900142
1113	9	-0.2440397767667335
1113	10	-0.21564824793524195
1113	11	-0.19706709427725855
1113	12	-0.3548284024090478
1113	13	-0.3533773856168736
1113	14	0.10231308420020468
1113	15	-0.2672170509145033
1113	16	-0.32336003921668777
1113	17	-0.11051638529855978
1113	18	-0.2914365745384089
1113	19	-0.7477977321632423
1113	20	-0.439611455842126
1113	21	-0.18737778749348166
1113	22	-0.8412652237736964
1113	23	-4.781009931036223
1113	24	-2.1725095402511805
1113	25	-1.1794006862341746
1114	1	-0.7465370451650557
1114	2	-0.8962710684862643
1114	3	-0.8111543832353867
1114	4	-0.7284145403622269
1114	5	-0.6287936735415158
1114	6	-1.3087963269884142
1114	7	-1.1264331575832287
1114	8	-1.0654660637492626
1114	9	-1.2411815438642506
1114	10	-0.9126766510963478
1114	11	-1.193337019891203
1114	12	-2.04075643908039
1114	13	-1.240190980889892
1114	14	-3.2316518365200366
1114	15	-1.9404997045414518
1114	16	-1.8444605393324522
1114	17	-1.7382223459181603
1114	18	-1.6651542762475415
1114	19	-1.7740894660860533
1114	20	-1.367810660031111
1114	21	-1.1764035475868155
1114	22	-1.3582260449027501
1114	23	-5.483877926818003
1114	24	-2.7110794584202544
1114	25	-2.712221413863201
1115	1	-1.5539854346487587
1115	2	-1.514882323108239
1115	3	-1.1658934994121353
1115	4	-1.0783926555189502
1115	5	-1.1756341320877794
1115	6	-1.1807305226846019
1115	7	-1.7924681511637672
1115	8	-2.1420907261157396
1115	9	-3.345427744160716
1115	10	-4.284978387798564
1115	11	-2.9162911249822514
1115	12	-3.0970137334649195
1115	13	-2.406126891146627
1115	14	-3.166030154223076
1115	15	-2.7188193541027985
1115	16	-2.684669957952604
1115	17	-2.6927443168679552
1115	18	-5.586723539816482
1115	19	-3.3753883092308
1115	20	-3.534584250175442
1115	21	-3.3984173749147253
1115	22	-2.7109556485397164
1115	23	-3.0668717679716195
1115	24	-2.717992176150112
1115	25	-2.8716425617860892
1116	1	-1.7039974883541653
1116	2	-1.4774715574273536
1116	3	-1.9364672065907342
1116	4	-1.6651088161759837
1116	5	-1.7881394824570158
1116	6	-4.11446876212203
1116	7	-2.572281392528147
1116	8	-2.845539233834213
1116	9	-4.037024261541831
1116	10	-3.50456410112373
1116	11	-3.0345683142877427
1116	12	-2.8218161592936326
1116	13	-4.19024502729938
1116	14	-7.117395502853956
1116	15	-5.093429658626956
1116	16	-3.9112132012357357
1116	17	-4.7737107780476435
1116	18	-3.6985983255967163
1116	19	-3.439053193261035
1116	20	-4.108111033180865
1116	21	-5.017544883438418
1116	22	-4.389860516104754
1116	23	-3.4785722260124246
1116	24	-4.325222595438664
1116	25	-4.162225419941034
1201	1	0.5513315637201863
1201	2	0.543561950502415
1201	3	0.9296782446788434
1201	4	0.914899677704561
1201	5	0.5365841446873674
1201	6	0.9468030034468669
1201	7	0.9972513153467091
1201	8	0.9643968904681515
1201	9	1.4673839766643297
1201	10	1.2176272342601422
1201	11	1.5190413704520154
1201	12	1.3248843113016662
1201	13	1.3828165018565102
1201	14	1.6006698657075304
1201	15	1.537641213797535
1201	16	1.4270036844271914
1201	17	1.6355835863913057
1201	18	1.4226471003628092
1201	19	1.6983293187826902
1201	20	1.7170051142008305
1201	21	1.7478313997595691
1201	22	1.5594880718948545
1201	23	1.846348733710279
1201	24	1.6864629250689092
1201	25	1.7797133297425916
1202	1	0.9936181589882516
1202	2	0.9820362061629346
1202	3	1.0732592706608557
1202	4	1.1882226399798412
1202	5	1.0187337973823745
1202	6	1.2504638196658604
1202	7	1.280815697453292
1202	8	1.341216042355363
1202	9	1.652674080528243
1202	10	1.3907413814148626
1202	11	1.3698004510521429
1202	12	1.56049858042784
1202	13	1.583952718176704
1202	14	1.9589592544702654
1202	15	1.5984482326619869
1202	16	1.4278949820779516
1202	17	1.767757703074075
1202	18	1.7282398049368126
1202	19	1.7396687186273994
1202	20	1.6443909802887262
1202	21	1.7391262626009336
1202	22	1.7580439299396033
1202	23	1.9093807905949731
1202	24	1.8129061957995276
1202	25	2.0422020212468226
1203	1	0.848797908895488
1203	2	1.0570524311486764
1203	3	1.0444473273239367
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2211	18	-0.024296291925587354
2211	19	0.152006509756049
2211	20	-0.01741284955281941
2211	21	0.33163952494451365
2211	22	0.28438795401218186
2211	23	0.48348706150648724
2211	24	0.06990840828144229
2211	25	0.46682800548285996
2212	1	0.01618841714707031
2212	2	0.1316186788332665
2212	3	-0.18572284447247256
2212	4	0.09496453727746967
2212	5	-0.1374740657595126
2212	6	-0.2971057036448528
2212	7	0.019464699262350393
2212	8	0.006166727367400426
2212	9	-0.006291467120639993
2212	10	-0.3474410586549297
2212	11	-0.35109943057051396
2212	12	-0.013863504616800526
2212	13	-0.026792097135924564
2212	14	-0.09190051780698383
2212	15	-0.35952780861777
2212	16	-0.44278698368079716
2212	17	-0.12746685314447603
2212	18	0.1333992402528068
2212	19	-0.13587152833500227
2212	20	0.03900841836775015
2212	21	0.13806915020658295
2212	22	0.22937256202654055
2212	23	0.27612022949168846
2212	24	0.27385610053649145
2212	25	0.3344265266940525
2213	1	-0.5907964084466393
2213	2	-0.47866460879454564
2213	3	-0.6307672271101801
2213	4	-0.5553267785868741
2213	5	-0.4658330968111173
2213	6	-0.31147183020259916
2213	7	-0.5767904791450711
2213	8	-0.34043874456429535
2213	9	-1.0835712979301064
2213	10	-0.46282916153587195
2213	11	-0.5736130480445283
2213	12	-0.5329316056270841
2213	13	-0.6468950352366605
2213	14	-0.7832188503760378
2213	15	-0.701827521653783
2213	16	-0.6763043515623934
2213	17	-0.7699221944549492
2213	18	-0.7521040769157921
2213	19	-0.6524421722697227
2213	20	-0.5582371690250554
2213	21	-0.8006334452001695
2213	22	-0.9187511299749715
2213	23	-0.696868521883701
2213	24	-0.46544718363580273
2213	25	-0.18784494461669254
2214	1	-2.5281956514503214
2214	2	-1.2783357815435288
2214	3	-1.4657877944268805
2214	4	-3.2545392727999065
2214	5	-1.58833553251932
2214	6	-1.4801741890524447
2214	7	-1.300879354190716
2214	8	-1.0657834776675514
2214	9	-0.904857022314772
2214	10	-0.9251885003839462
2214	11	-0.8044618438505893
2214	12	-0.6881866759662998
2214	13	-1.3239160086376103
2214	14	-1.0642143999265556
2214	15	-1.4948242161067498
2214	16	-1.333991010791273
2214	17	-3.6420011385083413
2214	18	-3.0902405701231856
2214	19	-2.070998362051391
2214	20	-2.0001668558564987
2214	21	-1.7946167277109595
2214	22	-2.852220418259172
2214	23	-1.7292541392726122
2214	24	-2.844497860717503
2214	25	-2.8849920698963416
2215	1	-2.111455093561247
2215	2	-3.611926587334729
2215	3	-2.2713930755220453
2215	4	-4.015039696718752
2215	5	-2.580829659448881
2215	6	-2.421310048701038
2215	7	-2.6551273664133923
2215	8	-1.988428002692256
2215	9	-4.366860563696644
2215	10	-2.6714821112712386
2215	11	-2.2112846975826663
2215	12	-2.074709591599703
2215	13	-2.156626865763201
2215	14	-2.03893375269881
2215	15	-2.360774924949787
2215	16	-2.354443003803489
2215	17	-2.6088677387223775
2215	18	-2.353149182286824
2215	19	-2.512311911425929
2215	20	-2.247777556420843
2215	21	-2.807223793528749
2215	22	-2.8037545673372115
2215	23	-2.6656294793386337
2215	24	-2.7135069375998135
2215	25	-3.499050929584328
2216	1	-3.253397130033356
2216	2	-3.3229664683709004
2216	3	-4.577859827309624
2216	4	-5.1716730558460355
2216	5	-4.857642159008172
2216	6	-4.058000669879117
2216	7	-5.376326260241989
2216	8	-3.3083462176706995
2216	9	-4.16566316892931
2216	10	-3.1309204908434722
2216	11	-3.488825965511662
2216	12	-3.7829734671222504
2216	13	-3.366060217995848
2216	14	-4.4749464273401
2216	15	-3.686508400737466
2216	16	-4.147157304926246
2216	17	-3.7826465457046616
2216	18	-3.451216124512637
2216	19	-4.130804216201273
2216	20	-4.744244446623487
2216	21	-4.3827523236156125
2216	22	-4.081675040309321
2216	23	-4.319273334245175
2216	24	-4.689596649364805
2216	25	-4.7331609455334025
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88789.0
3	1.0
4	866.0
5	4940.0
6	6013.0
7	5596.0
8	4760.0
9	4411.0
10	3895.0
11	3848.0
12	3457.0
13	3470.0
14	3433.0
15	3509.0
16	3621.0
17	3869.0
18	4366.0
19	5387.0
20	6864.0
21	8881.0
22	11061.0
23	13331.0
24	15628.0
25	17353.0
26	19540.0
27	21117.0
28	24370.0
29	28091.0
30	34204.0
31	42355.0
32	53051.0
33	67284.0
34	87343.0
35	113769.0
36	153143.0
37	232895.0
38	642950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.484303828139936	17.81737858742779	12.211221501111332	24.487096083320942
2	15.863915007546098	19.760299119593466	39.967984176376625	24.407801696483812
3	19.57963354575813	25.07787796495706	28.522536392174146	26.81995209711066
4	12.849011735550908	15.853438679528747	36.87899018746548	34.41855939745487
5	13.804137618422688	38.356731227646854	33.8423465162425	13.996784637687961
6	32.89346404367804	35.10949578161896	17.990789491016574	14.006250683686424
7	29.459737766583167	31.377979677883804	20.93886985575673	18.223412699776297
8	26.87655603917925	34.67402375548373	20.285938848018294	18.163481357318727
9	28.117008487433797	14.532704312102055	19.18501391471787	38.165273285746274
10	16.829795253003958	28.099013553190343	32.40914803117314	22.662043162632557
11	35.45166343535126	22.242523347089097	23.278758945779767	19.02705427177988
12	24.380784027091988	24.37849275614691	29.937746168422162	21.302977048338942
13	29.533033905042057	21.245090189313366	25.308678036694282	23.913197868950295
14	22.615929448866524	21.290145946729243	26.549806948922367	29.544117655481866
15	24.50654710698171	29.119133177010724	23.02719074069195	23.347128975315616
16	23.626488178765882	27.197830835900263	25.930709548972864	23.24497143636099
17	22.896815622296945	27.402432248197005	26.38238882746848	23.318363302037568
18	22.721132860162573	26.532792566750874	28.014573201120484	22.731501371966065
19	24.244397818068926	26.227794475060907	26.845278907636818	22.682528799233356
20	24.95479193981958	25.83741594909432	26.850981517535917	22.356810593550183
21	24.79451968493114	25.678009153331182	26.268605769313375	23.258865392424305
22	24.815171675811175	26.288757236459958	26.15969533830486	22.736375749424003
23	23.914331047835077	26.24221699058879	26.62101398220863	23.22243797936751
24	23.967021202864625	25.955738440151464	26.91882287643433	23.158417480549577
25	23.928676368247682	26.169639630196674	26.85994223040138	23.041741771154264
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	235.0
1	235.0
2	569.0
3	903.0
4	903.0
5	903.0
6	1487.5
7	2072.0
8	2072.0
9	2072.0
10	2413.5
11	2755.0
12	2755.0
13	2755.0
14	3869.0
15	4983.0
16	4983.0
17	4983.0
18	9501.0
19	14019.0
20	14019.0
21	14019.0
22	22829.0
23	31639.0
24	31639.0
25	31639.0
26	48354.0
27	65069.0
28	65069.0
29	65069.0
30	84852.5
31	104636.0
32	104636.0
33	104636.0
34	120951.5
35	137267.0
36	137267.0
37	137267.0
38	153493.0
39	169719.0
40	169719.0
41	169719.0
42	184482.5
43	199246.0
44	199246.0
45	199246.0
46	217402.0
47	235558.0
48	235558.0
49	235558.0
50	234110.0
51	232662.0
52	232662.0
53	232662.0
54	212569.5
55	192477.0
56	192477.0
57	192477.0
58	169879.0
59	147281.0
60	147281.0
61	147281.0
62	125043.5
63	102806.0
64	102806.0
65	102806.0
66	80085.5
67	57365.0
68	57365.0
69	57365.0
70	42652.5
71	27940.0
72	27940.0
73	27940.0
74	20008.5
75	12077.0
76	12077.0
77	12077.0
78	8312.5
79	4548.0
80	4548.0
81	4548.0
82	3066.5
83	1585.0
84	1585.0
85	1585.0
86	1034.5
87	484.0
88	484.0
89	484.0
90	293.0
91	102.0
92	102.0
93	102.0
94	63.5
95	25.0
96	25.0
97	25.0
98	16.5
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3352292268611431
2	0.011445176745003179
3	0.010071755535602798
4	0.011788532047353275
5	0.01281859795440356
6	0.08080294781972244
7	0.07914339719169698
8	0.1054673037052043
9	0.08400759730832334
10	0.09150418807630042
11	0.08732669856437425
12	0.0975701317511521
13	0.08395037142459831
14	0.08870011977377464
15	0.08578159970379882
16	0.0878417315178994
17	0.10020252240250282
18	0.10249155775150347
19	0.0954527740533265
20	0.09407935284392613
21	0.08183301372677272
22	0.10226265421660341
23	0.09562445170450157
24	0.09224812456472561
25	0.0892723786110248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1747461.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.39060109804177
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.70855479925896	42.69250913394249
2	13.58215592512842	15.318118736506426
3	4.645778064913964	7.8593465294578735
4	2.1362004616993397	4.818465124045606
5	1.1571593191821599	3.262645478744139
6	0.6814422087230528	2.3056161458082114
7	0.4236961978904057	1.6724738297396384
8	0.3263433064552399	1.4722156172266747
9	0.2199219769634583	1.11613792280752
>10	1.0162070953027478	9.964536719849502
>50	0.06023987353654949	2.352495652587704
>100	0.03677057265306597	3.9034754954087925
>500	0.003994032100266878	1.5834533731472675
>1k	0.0014337551129163153	1.3719720553579502
>5k	1.0241107949402251E-4	0.3065381853702066
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5308	0.30375499081238433	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3000	0.17167765117504769	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2879	0.16475331924432077	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2601	0.14884452356876632	No Hit
GTCCTACAGTGGACATTTCTAAATT	1787	0.10226265421660341	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1755	0.10043142593740288	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.72258837250159E-5	0.0	0.0	0.0
3	0.0	5.72258837250159E-5	0.0	0.0	0.0
4	0.0	5.72258837250159E-5	0.0	0.0	5.72258837250159E-5
5	0.0	5.72258837250159E-5	0.0	0.0	5.72258837250159E-5
6	0.0	5.72258837250159E-5	0.0	0.0	5.72258837250159E-5
7	0.0	5.72258837250159E-5	0.0	0.0	5.72258837250159E-5
8	0.0	5.72258837250159E-5	0.0	0.0	5.72258837250159E-5
9	0.0	5.72258837250159E-5	0.0	5.72258837250159E-5	5.72258837250159E-5
10	0.0	5.72258837250159E-5	0.0	5.72258837250159E-5	5.72258837250159E-5
11	0.0	5.72258837250159E-5	0.0	5.72258837250159E-5	5.72258837250159E-5
12	0.0	5.72258837250159E-5	0.0	5.72258837250159E-5	5.72258837250159E-5
13	0.0	5.72258837250159E-5	0.0	5.72258837250159E-5	5.72258837250159E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	55	1.9628035E-4	13.81616	5
GGTATCA	1455	0.0	12.95489	1
TGTACCG	95	1.0418444E-6	11.998245	5
TCCAACG	175	0.0	11.941794	18
CGTTTCC	130	2.6193447E-9	11.691268	14
GTCGATC	65	8.0322125E-4	11.690932	8
ATCCCGT	115	7.086237E-8	11.563857	10
TCCCGTT	150	1.7644197E-10	11.398659	11
CCAACGA	135	4.7384674E-9	11.257613	19
ACAACGC	60	0.005881297	11.082029	8
TCTATAC	120	1.2779674E-7	11.081713	3
TAGGACC	310	0.0	11.030644	4
CCGCCCA	70	0.001493026	10.855866	9
CGCCCTA	350	0.0	10.855866	16
GTCCTAA	590	0.0	10.649359	1
TTAGGAC	755	0.0	10.56799	3
CCCGTTT	145	1.4451871E-8	10.481826	12
TTAGACT	100	2.4027126E-5	10.448472	4
CCGTTTC	165	9.295036E-10	10.362714	13
GGCGAGG	440	0.0	10.362121	19
>>END_MODULE