FastQCFastQC Report
Thu 26 May 2016
SRR1512923_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512923_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1747461
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64660.37002256416595275No Hit
TATCAACGCAGAGTACTTTTTTTTT47220.27022062294952504No Hit
GGTATCAACGCAGAGTACTTTTTTT37170.21270860980588407No Hit
GTACTGGTTCACTATCGGTCAGTCA23650.1353392150096626No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22140.1266981065671852No Hit
GTCCTAAAGTGTGTATTTCTCATTT17500.10014529651877781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.013.6147631
GCGCATA500.00150717113.29120411
TAGGACC3150.011.7740084
GTATTAG1104.8905895E-711.2410821
GTCTAAT951.3419414E-511.0135291
GTCCTAG2350.010.5235661
TTATACT1451.4202669E-810.4935464
GTCTAAG1553.588866E-910.4321691
TCTATAC1659.113137E-1010.3754913
GGCGAGG4600.010.31719219
GTCCTAA6400.010.2547851
CCGTGTA750.002651693810.1315629
GTACCGT856.533479E-410.0688856
CGTGCGC1701.5661499E-910.05591310
CTATAAG1909.640644E-1110.0122991
AGGCCGT1054.0601466E-59.9623366
GACGTGA5450.09.9414447
GTATCAA30750.09.8983381
GTCCTAC9650.09.8566681
TATACAC3900.09.7547343
Produced by FastQC (version 0.11.5)