FastQCFastQC Report
Thu 26 May 2016
SRR1512922_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512922_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487139
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT104990.4221316138744155No Hit
TATCAACGCAGAGTACTTTTTTTTT60950.24506069021474072No Hit
GTCCTACAGTGGACATTTCTAAATT59140.23778325216242435No Hit
GGTATCAACGCAGAGTACTTTTTTT57100.229581056788543No Hit
CTGTAGGACGTGGAATATGGCAAGA48190.19375676228791394No Hit
GTCCTAAAGTGTGTATTTCTCATTT43970.1767894757791985No Hit
GTCCTACAGTGTGCATTTCTCATTT37810.15202206229728213No Hit
CTTTAGGACGTGAAATATGGCGAGG37770.15186123493700995No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30230.12154527752570322No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATGT400.005285626714.24782712
TAGGACC10850.013.4815434
TCCAACG2250.013.08741918
GGTATCA22700.012.8422081
TACGGCG550.003071916812.08955116
TGTAGGA31250.012.0059272
CTGTAGG30300.011.7905141
CCAACGA1701.8189894E-1211.73374119
GGCGAGG11350.011.46544219
GTCCTAC28450.011.3852021
GTAGGAC31300.011.3798243
AGGACCT19350.011.24095255
GGACCTG19150.011.1601776
CGGACGA600.005882251511.08208917
ACCCGTC600.00588401411.0816437
TGGCGAG26200.010.91289618
AGGACGT28500.010.6981535
GTCCTAA13850.010.661691
GTATCAA48500.010.5874441
GGACGTG27900.010.5539466
Produced by FastQC (version 0.11.5)