##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512922_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2487139
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.927443942618407	31.0	30.0	34.0	22.0	34.0
2	28.94999837162298	31.0	30.0	34.0	20.0	34.0
3	29.147603732642203	31.0	30.0	34.0	22.0	34.0
4	32.578292970356706	35.0	33.0	37.0	25.0	37.0
5	32.73476311537071	35.0	33.0	37.0	26.0	37.0
6	32.6236720183311	37.0	33.0	37.0	25.0	37.0
7	32.51030119345963	36.0	33.0	37.0	23.0	37.0
8	32.44360528301796	36.0	33.0	37.0	23.0	37.0
9	33.70875130018869	38.0	34.0	39.0	22.0	39.0
10	33.60426618697226	38.0	34.0	39.0	19.0	39.0
11	33.66657392288891	38.0	34.0	39.0	20.0	39.0
12	33.56883149675189	38.0	34.0	39.0	18.0	39.0
13	33.533511396025716	38.0	34.0	39.0	18.0	39.0
14	34.42113770078793	39.0	34.0	41.0	17.0	41.0
15	34.58246684242417	39.0	34.0	41.0	18.0	41.0
16	34.500865050164066	39.0	34.0	41.0	17.0	41.0
17	34.30209931974047	39.0	34.0	41.0	17.0	41.0
18	34.26862350676822	39.0	34.0	41.0	17.0	41.0
19	34.39024960004246	39.0	34.0	41.0	18.0	41.0
20	34.391526569283016	39.0	34.0	41.0	18.0	41.0
21	34.316422202377915	39.0	34.0	41.0	16.0	41.0
22	34.29028735426528	39.0	34.0	41.0	16.0	41.0
23	33.97560811840432	39.0	34.0	41.0	10.0	41.0
24	34.03464301753943	39.0	34.0	41.0	2.0	41.0
25	33.76493030747377	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.49142661799362486
1101	2	0.08171345084348758
1101	3	0.4566559557204535
1101	4	0.5437978441645797
1101	5	0.4646734816139215
1101	6	0.37193032905489076
1101	7	0.09830170688523054
1101	8	0.3805924990469407
1101	9	0.3939280724041936
1101	10	0.40491867257094327
1101	11	0.4684994992841993
1101	12	0.22585649611642822
1101	13	0.7058126475574653
1101	14	0.713728175040238
1101	15	0.5434181851273152
1101	16	0.4065290530496455
1101	17	-0.2000737922345266
1101	18	0.27159035151590416
1101	19	-0.26517073079535436
1101	20	-0.8619338254637725
1101	21	0.15510722205889493
1101	22	0.25190674430233173
1101	23	-0.11615530953405084
1101	24	0.29664183560944224
1101	25	-0.06283440742178925
1102	1	0.8263241368216967
1102	2	0.8354422270396213
1102	3	0.8163854139598357
1102	4	0.9101307548537108
1102	5	0.7942786416451995
1102	6	0.6588999948258731
1102	7	0.7107769147986644
1102	8	0.7851338682472147
1102	9	1.0342162990577393
1102	10	1.1091878737424778
1102	11	0.7829159333728839
1102	12	0.918976485332351
1102	13	0.8358376692075495
1102	14	1.1553389348753669
1102	15	1.0667288218174704
1102	16	1.0383911084113961
1102	17	1.235858138241582
1102	18	1.1891320648295647
1102	19	1.0416721788147498
1102	20	1.1936087343750117
1102	21	1.2206257310616522
1102	22	1.0567360804479335
1102	23	1.0174565917805367
1102	24	0.41389329407204656
1102	25	0.7341506893458032
1103	1	0.6203757659200377
1103	2	0.6287925088507222
1103	3	0.8196976410203476
1103	4	0.870142105724014
1103	5	0.670874206723667
1103	6	0.321780463755708
1103	7	0.21523377774977348
1103	8	0.3921419530482737
1103	9	0.5702594132073244
1103	10	0.7345161794065689
1103	11	0.5624843069506156
1103	12	0.7370090430528151
1103	13	0.8615871424487409
1103	14	0.9535773348815937
1103	15	0.7811248205159558
1103	16	0.7475416999168658
1103	17	0.4556275108276111
1103	18	0.8497259560138701
1103	19	0.763431336216172
1103	20	1.0430384219895998
1103	21	0.7637512853969071
1103	22	0.806822124953996
1103	23	0.05944906464486621
1103	24	0.3848848758092416
1103	25	0.5448335585416899
1104	1	0.6320398963506193
1104	2	0.5979405861103864
1104	3	0.5344047495834339
1104	4	0.7277205188039204
1104	5	0.4834758678914426
1104	6	0.3752145452415405
1104	7	0.4769101659118675
1104	8	0.2773139609657349
1104	9	0.5856800257491912
1104	10	0.5149912326199768
1104	11	0.30184624522335923
1104	12	0.6156328370777544
1104	13	0.6627618045736554
1104	14	0.8646059947950704
1104	15	0.49612736269517654
1104	16	0.6836687856998864
1104	17	0.8541673995660446
1104	18	0.7597935503456625
1104	19	0.32101275058306555
1104	20	0.39016079694068395
1104	21	0.5089216351775931
1104	22	0.32178556125524693
1104	23	0.7221071410932893
1104	24	0.5427462716216453
1104	25	-0.06465882873843753
1105	1	0.3895946679144231
1105	2	0.5208297315989512
1105	3	0.4974931140552634
1105	4	0.6553683581467098
1105	5	0.39597951086798844
1105	6	0.6412092958461315
1105	7	0.46217479003184536
1105	8	0.573682553048215
1105	9	0.2511736875987367
1105	10	0.29872654059720105
1105	11	0.3151201024867518
1105	12	0.5473126757767304
1105	13	0.5088339901063321
1105	14	0.6087333236023653
1105	15	0.516314789634599
1105	16	0.5090867052200352
1105	17	0.8717899019813871
1105	18	0.6016462288713953
1105	19	0.45856318128042517
1105	20	0.6559637703815611
1105	21	0.5584112558454066
1105	22	0.4982988067758143
1105	23	0.6905815253900442
1105	24	0.8146980455428618
1105	25	1.0207292077002776
1106	1	0.64850468724579
1106	2	0.6381982191323274
1106	3	0.4637809376500961
1106	4	0.6971062260940357
1106	5	0.5060417519083771
1106	6	0.6158442170495846
1106	7	0.562854675122928
1106	8	0.5809551238963309
1106	9	0.7752291943431189
1106	10	0.5069802388642586
1106	11	0.2591737897943531
1106	12	0.48667961768130397
1106	13	0.4436089662939082
1106	14	0.8357633952987698
1106	15	0.49450766766408805
1106	16	0.5960106843556545
1106	17	0.7981152066879673
1106	18	0.7737851779140144
1106	19	0.7094572071793763
1106	20	0.8911149677797994
1106	21	0.8026654679337852
1106	22	0.7744478409006064
1106	23	1.035088727247988
1106	24	0.6917411858883753
1106	25	0.7950040374939888
1107	1	0.7212112345903954
1107	2	0.7345877961994702
1107	3	0.7010067673178746
1107	4	0.7485386785514336
1107	5	0.6435318373606407
1107	6	0.732825443951107
1107	7	0.3366135579863254
1107	8	0.6467769346653114
1107	9	0.6379574563255233
1107	10	0.8975187926661121
1107	11	0.8479589874591227
1107	12	0.7673912192588617
1107	13	0.6532107845304225
1107	14	0.945638273181622
1107	15	0.7549237440055876
1107	16	0.8098509498847903
1107	17	1.1403429475517157
1107	18	1.069660352912635
1107	19	0.9222337213211773
1107	20	1.0615100407888889
1107	21	0.837647700457886
1107	22	0.8410892853776204
1107	23	1.3174878717225127
1107	24	1.0625836933121846
1107	25	1.2598229309779896
1108	1	0.7220833651439946
1108	2	0.661184178708055
1108	3	0.7359687085611846
1108	4	0.8099202104054086
1108	5	0.7071231834462424
1108	6	0.6894174734112468
1108	7	0.5580662797569076
1108	8	0.6338538220734407
1108	9	0.7038008628427974
1108	10	0.7453400378407551
1108	11	0.4543097404215928
1108	12	0.6270637064379869
1108	13	0.682352044332383
1108	14	0.7771479225516842
1108	15	0.6859497250806399
1108	16	0.4848372452174985
1108	17	0.9345395701539019
1108	18	0.8648862619325683
1108	19	0.7187547298495005
1108	20	0.6992238657057612
1108	21	0.7344574702127176
1108	22	0.7212342810955477
1108	23	1.059868685053111
1108	24	0.9180055368282041
1108	25	0.9952321073933845
1109	1	0.5547854504678895
1109	2	0.5563553311429033
1109	3	0.6763742076951509
1109	4	0.7838370773869485
1109	5	0.6184862380544942
1109	6	0.6804549672854492
1109	7	0.5766607448493204
1109	8	0.47164982702895486
1109	9	0.6490700106701368
1109	10	0.7864451369006673
1109	11	0.6388890895988197
1109	12	0.5352502029750639
1109	13	0.6896005427956808
1109	14	0.8916606529224751
1109	15	0.6722508618132963
1109	16	0.35752954315822194
1109	17	0.8063330126071833
1109	18	0.7947628568540352
1109	19	0.7087183206451897
1109	20	0.6696295350248889
1109	21	0.6733768014058796
1109	22	0.7347837982038428
1109	23	0.9800478518196343
1109	24	0.7158389408404773
1109	25	0.4906945890333603
1110	1	0.4992298949123324
1110	2	0.5874082180909852
1110	3	0.5048555274428459
1110	4	0.4898050540972818
1110	5	0.41834067764692406
1110	6	0.4439193049855916
1110	7	0.4560882072462178
1110	8	0.4786852467281264
1110	9	0.5711951926206496
1110	10	0.5525780820729196
1110	11	0.49138787659542515
1110	12	0.5189959574631757
1110	13	0.46762092125274535
1110	14	0.4945233409380023
1110	15	0.3394027298385325
1110	16	0.5792665640218786
1110	17	0.65179637990461
1110	18	-0.02571264047735866
1110	19	0.3632549533477629
1110	20	0.4767619949957762
1110	21	0.6217028567188336
1110	22	0.5940754042203409
1110	23	0.6777674054343805
1110	24	0.5955090039166535
1110	25	0.7864248170780002
1111	1	0.1779075716173324
1111	2	0.23541160208369405
1111	3	0.32885030150610817
1111	4	0.1407516934186006
1111	5	0.13534003776767634
1111	6	-0.032797179077206806
1111	7	-0.015635953357204357
1111	8	0.20087891030348715
1111	9	0.31289618583480916
1111	10	0.327654495669762
1111	11	-0.049737934827867036
1111	12	-0.2827833231025281
1111	13	0.08488639119976682
1111	14	0.0341316416535733
1111	15	-0.03906615536789104
1111	16	0.07553939453145375
1111	17	-0.3208449094183692
1111	18	-0.002030781674854154
1111	19	-0.058462247933228184
1111	20	0.046467384467725026
1111	21	0.11147690992874004
1111	22	-0.12992129740376157
1111	23	0.23549525110841785
1111	24	0.20106069843775742
1111	25	0.1407462724975943
1112	1	0.22785014126817416
1112	2	0.12103750988380213
1112	3	0.05173996474098885
1112	4	0.1831388759848025
1112	5	0.17076165520552422
1112	6	-0.11774928476444302
1112	7	-0.22517245892135662
1112	8	-0.08880264598920462
1112	9	-0.1160035622367559
1112	10	-0.04164833309371829
1112	11	-0.11510591852698582
1112	12	-0.027622050164971768
1112	13	-0.01165097016561134
1112	14	-0.1465648799727859
1112	15	-0.42035266736799315
1112	16	-0.09971561955666175
1112	17	0.08365193263421133
1112	18	-0.12648592116179458
1112	19	0.027067529594546613
1112	20	-0.20995431260751474
1112	21	-0.130453247837508
1112	22	-0.26108504511539365
1112	23	0.04281149744925017
1112	24	-0.05652115424557991
1112	25	-0.10415988169242496
1113	1	-0.1134496317669651
1113	2	0.05392142630182306
1113	3	0.12295578937197504
1113	4	0.13478579467550134
1113	5	-0.0396189442965138
1113	6	-0.30245975083320786
1113	7	-0.16920375623470107
1113	8	-0.1434697612377036
1113	9	-0.1904446337416914
1113	10	-0.11052295784506327
1113	11	-0.20266984333630234
1113	12	-0.33937754708491497
1113	13	-0.14884058894612195
1113	14	0.1691255560482503
1113	15	-0.017234323500410653
1113	16	-0.09071224557682456
1113	17	0.07118771774816679
1113	18	-0.07266641301431065
1113	19	-0.5755671379534277
1113	20	-0.30055869101252597
1113	21	-0.02126332184844415
1113	22	-0.43832413105103996
1113	23	-4.080891311939993
1113	24	-1.8148868300929877
1113	25	-0.8733711073052177
1114	1	-0.3989160893836754
1114	2	-0.5580385362599536
1114	3	-0.37583700675066467
1114	4	-0.19773478740541606
1114	5	-0.2026182944929502
1114	6	-0.9040943943056661
1114	7	-0.8095881537952536
1114	8	-0.6209621156131497
1114	9	-0.7473203304920801
1114	10	-0.45270713244549654
1114	11	-0.6556425456249215
1114	12	-1.6601241654236851
1114	13	-0.8116104213683428
1114	14	-2.6164452278294696
1114	15	-1.3345053869725092
1114	16	-1.3148110157192434
1114	17	-1.1100299783703065
1114	18	-1.1161674831036734
1114	19	-1.2140855858171236
1114	20	-0.7504552589766149
1114	21	-0.7567276929399398
1114	22	-0.8933356665547549
1114	23	-5.22543981768986
1114	24	-2.3997577337968714
1114	25	-2.2795400681560416
1115	1	-1.1934638840250535
1115	2	-1.116182230409315
1115	3	-0.7504970967651694
1115	4	-0.7555292632127681
1115	5	-0.6569610357385542
1115	6	-0.750470878073692
1115	7	-1.2040187531286328
1115	8	-1.5545732772264564
1115	9	-2.8756204778180674
1115	10	-3.7771130553357466
1115	11	-2.3543543810632563
1115	12	-2.5173268977092746
1115	13	-1.9189905823271225
1115	14	-2.556546283587064
1115	15	-2.201306744524821
1115	16	-2.2723584311475733
1115	17	-2.2278785310298446
1115	18	-5.01995567026135
1115	19	-2.9209107087734125
1115	20	-3.074641300689091
1115	21	-2.6413937363033213
1115	22	-2.1675378134236993
1115	23	-2.6007277168583833
1115	24	-2.3189529606876107
1115	25	-2.4467828597687564
1116	1	-0.15569721170303907
1116	2	0.10263325605577833
1116	3	-0.3628796156029779
1116	4	0.25208837429180164
1116	5	0.0690294076889586
1116	6	-1.5714705833393623
1116	7	-0.31670715292967344
1116	8	-0.8328260816902038
1116	9	-1.8551892797548604
1116	10	-1.1650521964602234
1116	11	-0.5368839601722115
1116	12	-0.38216862421550246
1116	13	-1.8451932171014889
1116	14	-4.726154454850292
1116	15	-2.3002457009336723
1116	16	-1.2592366734423592
1116	17	-2.011930045263661
1116	18	-0.821182552580126
1116	19	-0.6410742139463181
1116	20	-1.1936605533841131
1116	21	-1.7039531246919495
1116	22	-1.5421662831387337
1116	23	-0.4434212296341613
1116	24	-1.3207930235951508
1116	25	-1.5900296797937798
1201	1	0.3296604667729959
1201	2	0.4505156715488816
1201	3	0.7599361745211795
1201	4	0.5636965994666312
1201	5	0.07957558690123534
1201	6	0.6003907097804841
1201	7	0.6364190057312697
1201	8	0.6500123683739361
1201	9	1.045286695008464
1201	10	1.00322522436241
1201	11	1.133108846356194
1201	12	1.012055562899505
1201	13	1.0472706881666412
1201	14	1.126904637047062
1201	15	1.1157788416175336
1201	16	1.1040371733447714
1201	17	1.2962620375793037
1201	18	1.1428606829082
1201	19	1.2716061966566343
1201	20	1.2166489374078893
1201	21	1.2990525206194548
1201	22	1.1096396767483299
1201	23	1.527321619898693
1201	24	1.4043211462827685
1201	25	1.465777610835083
1202	1	0.7731086570876542
1202	2	0.8241243213060301
1202	3	0.8242078866367706
1202	4	1.0297734272093138
1202	5	0.830574642736579
1202	6	1.0900981134077767
1202	7	1.0376219266094466
1202	8	1.0551648444727562
1202	9	1.3079702588119204
1202	10	1.203978310678771
1202	11	1.135415246782749
1202	12	1.3192347219970202
1202	13	1.2363574725437871
1202	14	1.6314105078238228
1202	15	1.4117282793486012
1202	16	1.1393597823561024
1202	17	1.5028285516673137
1202	18	1.5186992677705717
1202	19	1.5570165283838335
1202	20	1.335550994919764
1202	21	1.4364010479762612
1202	22	1.3653065156440292
1202	23	1.5045324052958549
1202	24	1.5648507426708846
1202	25	1.8894242565745287
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2211	7	-0.5348634430940784
2211	8	-0.6258767388382225
2211	9	-0.7619261383172784
2211	10	-0.6867737151391395
2211	11	-0.6002845818892624
2211	12	-0.6546809945980527
2211	13	-0.5724234899707099
2211	14	-0.2816130574691371
2211	15	-0.6363109751328153
2211	16	-0.6849037197990597
2211	17	-0.32398398561375075
2211	18	-0.7046415694062844
2211	19	-0.4973097598370373
2211	20	-0.6015248070901507
2211	21	-0.46111139858449945
2211	22	-0.45111935385651236
2211	23	-0.27851748207321236
2211	24	-0.5140623578381138
2211	25	-0.12317692440146999
2212	1	0.01728101168857421
2212	2	0.04717728263296195
2212	3	-0.2538202684852031
2212	4	0.0570679431837533
2212	5	-0.18019282005777626
2212	6	-0.3261630918654319
2212	7	-0.07084690028813867
2212	8	-0.12549954824092424
2212	9	0.046534147881878596
2212	10	-0.2474643457397292
2212	11	-0.4684691746861631
2212	12	0.10252656313476649
2212	13	0.10422063789903291
2212	14	0.03353751080799583
2212	15	-0.2595595363503662
2212	16	-0.3088390608429492
2212	17	-0.0600418293504319
2212	18	0.10627001751830534
2212	19	-0.15804390568874283
2212	20	-0.09586444437626085
2212	21	0.0710370580261852
2212	22	0.020982938530920592
2212	23	0.12864167808744753
2212	24	0.17205710329385226
2212	25	0.35828753155022497
2213	1	-0.2252434186410497
2213	2	-0.1987757909912311
2213	3	-0.36183199023775003
2213	4	-0.14325999288443825
2213	5	-0.15594781559335757
2213	6	0.004816910143752295
2213	7	-0.21387817576236046
2213	8	-0.042542126760636734
2213	9	-0.4717353343298427
2213	10	-0.05588797503010312
2213	11	-0.16183744798990318
2213	12	-0.18558248883628892
2213	13	-0.17338213896206156
2213	14	-0.2923056611612296
2213	15	-0.25162583010020256
2213	16	-0.22086865765464125
2213	17	-0.20565756005686353
2213	18	-0.11726433524826518
2213	19	-0.13905229440021571
2213	20	-0.010782685305620987
2213	21	-0.2369700369878487
2213	22	-0.34893835323401845
2213	23	-0.21712637712053606
2213	24	-0.04276185447322689
2213	25	0.17327409994939558
2214	1	-2.4939155569461455
2214	2	-1.2309454714664625
2214	3	-1.367304638702656
2214	4	-3.1634559306129866
2214	5	-1.590532491241074
2214	6	-1.374760031844854
2214	7	-1.2035302230816143
2214	8	-1.0435708480417674
2214	9	-0.8301374763399352
2214	10	-0.8938671967609295
2214	11	-0.8147626718390484
2214	12	-0.7935385853424393
2214	13	-1.1826447624561283
2214	14	-1.0330933787607819
2214	15	-1.5363304015673407
2214	16	-1.2572569355484973
2214	17	-3.34323625675281
2214	18	-3.0702768812680112
2214	19	-2.0032500667207387
2214	20	-1.7352468040196953
2214	21	-1.6408210273507322
2214	22	-2.6741624778430335
2214	23	-1.635011307434965
2214	24	-2.7237932477576834
2214	25	-2.520742954042973
2215	1	-1.4248748148756576
2215	2	-3.1055694258533464
2215	3	-1.7614835008398053
2215	4	-3.388993272598807
2215	5	-1.859572697443216
2215	6	-1.6651347645805146
2215	7	-1.7736614572400065
2215	8	-1.2466242258878992
2215	9	-3.5643269486703737
2215	10	-2.069968540182874
2215	11	-1.6538274743983585
2215	12	-1.5868043278148285
2215	13	-1.6082131848061287
2215	14	-1.327720226836746
2215	15	-1.5363137896485526
2215	16	-1.5438345051711977
2215	17	-1.943744259946584
2215	18	-1.4769575519608367
2215	19	-1.706173764426687
2215	20	-1.5946108354644508
2215	21	-2.1409646400679634
2215	22	-1.9721159966225414
2215	23	-1.9301225804973967
2215	24	-1.800793778267348
2215	25	-2.7225911639747586
2216	1	-1.4735493046925683
2216	2	-1.455847277624592
2216	3	-3.0253045524898567
2216	4	-3.3236626384758488
2216	5	-2.984210303870235
2216	6	-1.5982304519801076
2216	7	-2.969086942228209
2216	8	-1.2359367047307401
2216	9	-1.5595035840602876
2216	10	-0.6319915105450846
2216	11	-0.9013796562674941
2216	12	-1.493950885373117
2216	13	-0.9725710505133733
2216	14	-1.6079506839008388
2216	15	-1.301341951546675
2216	16	-1.3672102031403455
2216	17	-1.3414311128915415
2216	18	-0.8184376326379095
2216	19	-1.4925437049769101
2216	20	-2.0475133638501077
2216	21	-1.5674661070088263
2216	22	-1.2054665985612374
2216	23	-1.1425209493222113
2216	24	-1.5901400818462221
2216	25	-1.4381941994375609
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	126292.0
3	7.0
4	1151.0
5	7205.0
6	8621.0
7	7951.0
8	6903.0
9	6547.0
10	5667.0
11	5368.0
12	5050.0
13	4686.0
14	4819.0
15	4815.0
16	4977.0
17	5351.0
18	6048.0
19	7291.0
20	9233.0
21	12263.0
22	15478.0
23	18253.0
24	21412.0
25	24134.0
26	27151.0
27	29326.0
28	32798.0
29	38893.0
30	46828.0
31	58315.0
32	73420.0
33	93600.0
34	120832.0
35	159757.0
36	219322.0
37	341242.0
38	926133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.78185793937047	18.50524073491322	12.438817748299485	24.27408357741683
2	14.780153034574523	20.065230092698148	41.95430515797046	23.20031171475687
3	19.523232407714943	25.40361267016069	28.858215442914393	26.214939479209974
4	12.06994167773814	16.240843919590553	38.172652231397514	33.5165621712738
5	12.845448162770293	39.23461965014263	34.7369072904511	13.183024896635976
6	31.650400818295438	36.7582286253715	18.296041391198816	13.29532916513424
7	28.558650127232603	31.86215099556899	21.49764285702789	18.081556020170513
8	25.562409391051517	37.13483738485972	20.241707042403174	17.061046181685583
9	27.55055559445428	14.951152595926256	19.815531534924745	37.68276027469472
10	15.692534292193432	29.121351203974417	33.684723096951785	21.501391406880373
11	34.73318398552611	22.559231750801903	24.397883805689034	18.309700457982952
12	24.033608101875515	24.95699782433541	31.219981986173302	19.789412087615776
13	29.32159671642992	22.320671200354113	25.775019113918958	22.58271296929701
14	21.138498635005664	22.53484115844236	27.478237354486453	28.848422852065518
15	23.02341527956849	31.57380714385741	23.349083557474234	22.05369401909986
16	21.88508668538415	28.329941678561266	28.122820692460948	21.662150943593637
17	21.30618864978207	28.92228871779805	27.888928687854918	21.88259394456496
18	21.11366724407725	27.512379983386033	30.400506146524915	20.97344662601181
19	23.35262660555923	26.79595654224639	27.99830489648278	21.853111955711597
20	23.675348372250628	27.121684195614854	28.653451607788345	20.549515824346177
21	23.15058185388466	26.58601458256209	27.472194949218558	22.79120861433469
22	23.236460581317004	28.197674184608278	27.60257647605241	20.96328875802231
23	22.172304992796143	27.873212115358303	28.344561691578328	21.609921200267227
24	22.94263847405093	26.667004707622745	29.05771888353121	21.332637934795116
25	22.410144928702678	27.38795933593728	28.710483044701977	21.491412690658066
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	416.0
1	416.0
2	954.0
3	1492.0
4	1492.0
5	1492.0
6	2762.0
7	4032.0
8	4032.0
9	4032.0
10	5211.5
11	6391.0
12	6391.0
13	6391.0
14	8966.0
15	11541.0
16	11541.0
17	11541.0
18	20536.0
19	29531.0
20	29531.0
21	29531.0
22	46297.5
23	63064.0
24	63064.0
25	63064.0
26	91740.0
27	120416.0
28	120416.0
29	120416.0
30	155880.0
31	191344.0
32	191344.0
33	191344.0
34	214974.5
35	238605.0
36	238605.0
37	238605.0
38	255934.0
39	273263.0
40	273263.0
41	273263.0
42	286631.5
43	300000.0
44	300000.0
45	300000.0
46	312003.5
47	324007.0
48	324007.0
49	324007.0
50	312083.5
51	300160.0
52	300160.0
53	300160.0
54	266831.0
55	233502.0
56	233502.0
57	233502.0
58	201669.0
59	169836.0
60	169836.0
61	169836.0
62	140782.5
63	111729.0
64	111729.0
65	111729.0
66	86286.5
67	60844.0
68	60844.0
69	60844.0
70	44714.5
71	28585.0
72	28585.0
73	28585.0
74	20267.5
75	11950.0
76	11950.0
77	11950.0
78	8181.0
79	4412.0
80	4412.0
81	4412.0
82	2921.5
83	1431.0
84	1431.0
85	1431.0
86	929.5
87	428.0
88	428.0
89	428.0
90	269.5
91	111.0
92	111.0
93	111.0
94	69.5
95	28.0
96	28.0
97	28.0
98	24.5
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3409137969369625
2	0.010051710017011514
3	0.009046539015310363
4	0.010735226298168296
5	0.011941431500209678
6	0.07932809545425487
7	0.07747858081112474
8	0.10296971741426594
9	0.08254464265969856
10	0.09022414911269534
11	0.08491684622371326
12	0.09573248620201766
13	0.0819817468987459
14	0.08632408562609488
15	0.08423332994255649
16	0.08515808726412155
17	0.09766241452528387
18	0.09983358388895835
19	0.09388297155888754
20	0.09235511163630179
21	0.07956933649466315
22	0.10071813437045538
23	0.09484793572052065
24	0.08978187387194685
25	0.088173600269225
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2487139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.858851140394066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.28253312995774	48.25043881789459
2	12.34631222827991	15.027647560674282
3	3.836406845270241	7.004379393308713
4	1.6666516611296527	4.057220213903201
5	0.841336051357166	2.5601372754296357
6	0.48210236767564113	1.760411773728202
7	0.3303148598794063	1.4071808020802636
8	0.21250505161583155	1.0346250642295722
9	0.1500763096645158	0.8220124610615205
>10	0.7719324868740945	8.274197359714623
>50	0.04618676109226979	1.9379372292523764
>100	0.028672517881013954	3.4080486066689404
>500	0.0029155745358717946	1.2613458918899891
>1k	0.0017891025560970318	2.0564720812318944
>5k	1.9878917289967022E-4	0.7145529720040775
>10k+	6.62630576332234E-5	0.423392496928202
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10499	0.4221316138744155	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6095	0.24506069021474072	No Hit
GTCCTACAGTGGACATTTCTAAATT	5914	0.23778325216242435	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5710	0.229581056788543	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4819	0.19375676228791394	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4397	0.1767894757791985	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3781	0.15202206229728213	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3777	0.15186123493700995	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3023	0.12154527752570322	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.020684006804606E-5	0.0
5	0.0	0.0	0.0	4.020684006804606E-5	0.0
6	3.2165472054436847E-4	0.0	0.0	4.020684006804606E-5	0.0
7	3.2165472054436847E-4	0.0	0.0	4.020684006804606E-5	0.0
8	3.2165472054436847E-4	0.0	0.0	4.020684006804606E-5	0.0
9	3.2165472054436847E-4	0.0	0.0	4.020684006804606E-5	0.0
10	3.2165472054436847E-4	0.0	0.0	4.020684006804606E-5	0.0
11	3.618615606124145E-4	0.0	0.0	4.020684006804606E-5	0.0
12	3.618615606124145E-4	0.0	0.0	4.020684006804606E-5	0.0
13	3.618615606124145E-4	0.0	0.0	4.020684006804606E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATGT	40	0.0052856267	14.247827	12
TAGGACC	1085	0.0	13.481543	4
TCCAACG	225	0.0	13.087419	18
GGTATCA	2270	0.0	12.842208	1
TACGGCG	55	0.0030719168	12.089551	16
TGTAGGA	3125	0.0	12.005927	2
CTGTAGG	3030	0.0	11.790514	1
CCAACGA	170	1.8189894E-12	11.733741	19
GGCGAGG	1135	0.0	11.465442	19
GTCCTAC	2845	0.0	11.385202	1
GTAGGAC	3130	0.0	11.379824	3
AGGACCT	1935	0.0	11.2409525	5
GGACCTG	1915	0.0	11.160177	6
CGGACGA	60	0.0058822515	11.082089	17
ACCCGTC	60	0.005884014	11.081643	7
TGGCGAG	2620	0.0	10.912896	18
AGGACGT	2850	0.0	10.698153	5
GTCCTAA	1385	0.0	10.66169	1
GTATCAA	4850	0.0	10.587444	1
GGACGTG	2790	0.0	10.553946	6
>>END_MODULE