FastQCFastQC Report
Thu 26 May 2016
SRR1512922_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512922_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2487139
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT130310.5239353329267082No Hit
TATCAACGCAGAGTACTTTTTTTTT96310.3872320766953516No Hit
GGTATCAACGCAGAGTACTTTTTTT75730.3044863998353128No Hit
GTCCTACAGTGGACATTTCTAAATT53500.21510659436404642No Hit
ACGCAGAGTACTTTTTTTTTTTTTT49320.19830013521560316No Hit
CTGTAGGACGTGGAATATGGCAAGA46510.1870020131564822No Hit
GTCCTAAAGTGTGTATTTCTCATTT40710.1636820459170155No Hit
CTTTAGGACGTGAAATATGGCGAGG39360.15825412250782928No Hit
GTCCTACAGTGTGCATTTCTCATTT34760.1397589760765281No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26270.10562336885875699No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG1250.014.4546495
GGTATCA22150.013.8709581
CGCTAGA655.4732347E-513.14918110
TAGGACC9500.013.1132894
TCCAACG1751.8189894E-1211.39114518
CGTCCAA600.005892875611.07940210
ACCGCTA951.3611849E-510.999868
GGCGTGC1658.0035534E-1110.9392558
AGGCGTG2000.010.931687
CGGTTTC1408.451025E-910.84958313
GTATCAA49300.010.8048511
GATATAC7000.010.7351151
GGCGAGG10750.010.68450419
AGGACCT18700.010.5775645
GCCGGTT1355.7485522E-810.54841811
GTCTAGC1002.3664055E-510.4633411
GTCCTAA14050.010.4261051
GTAGGAC26450.010.3913293
GGACCGC1105.976024E-610.3741496
CTACACT4150.010.3116554
Produced by FastQC (version 0.11.5)