##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512922_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2487139
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.167309507027955	31.0	30.0	34.0	26.0	34.0
2	30.198395023358163	31.0	30.0	34.0	26.0	34.0
3	30.361839044781977	31.0	30.0	34.0	26.0	34.0
4	33.92675962220045	37.0	35.0	37.0	30.0	37.0
5	33.54473232095191	37.0	35.0	37.0	28.0	37.0
6	33.42120122759524	37.0	35.0	37.0	28.0	37.0
7	33.25263445267836	37.0	35.0	37.0	27.0	37.0
8	33.184203215019345	37.0	35.0	37.0	26.0	37.0
9	34.51870562923906	39.0	35.0	39.0	27.0	39.0
10	34.408828778769504	39.0	35.0	39.0	26.0	39.0
11	34.4038644402263	39.0	35.0	39.0	26.0	39.0
12	34.290028422215244	39.0	35.0	39.0	25.0	39.0
13	34.26438570582505	39.0	35.0	39.0	25.0	39.0
14	35.32638304493637	40.0	36.0	41.0	25.0	41.0
15	35.32594237796923	40.0	36.0	41.0	25.0	41.0
16	35.31665540205031	40.0	36.0	41.0	25.0	41.0
17	35.31252937612253	40.0	36.0	41.0	25.0	41.0
18	35.246067871558445	40.0	36.0	41.0	25.0	41.0
19	35.260531880204525	40.0	35.0	41.0	25.0	41.0
20	35.24370732797805	40.0	36.0	41.0	25.0	41.0
21	35.21315696468915	40.0	36.0	41.0	25.0	41.0
22	35.165915535882796	40.0	36.0	41.0	25.0	41.0
23	35.018432825829194	40.0	35.0	41.0	24.0	41.0
24	34.978891811032675	40.0	35.0	41.0	23.0	41.0
25	34.90886355768616	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8499705719871393
1101	2	0.6956452302286174
1101	3	0.5940080217736714
1101	4	0.6582415595323283
1101	5	0.710905562181054
1101	6	0.8023122255746173
1101	7	0.8192641953908435
1101	8	0.6946339310925609
1101	9	0.9075808188802839
1101	10	0.6980546206896534
1101	11	0.9603262812432689
1101	12	1.0727027972365164
1101	13	1.1322688389015596
1101	14	1.1977825441805834
1101	15	0.9296418575858922
1101	16	1.0763918957582135
1101	17	1.210482646774338
1101	18	0.9933733233419346
1101	19	0.8921235721843956
1101	20	1.0173194284632174
1101	21	1.0806437809162688
1101	22	1.0282467309740184
1101	23	1.217955519967262
1101	24	1.2114913693837082
1101	25	1.2474759392908865
1102	1	1.0963633639710615
1102	2	1.1364894798094518
1102	3	0.8591856328228964
1102	4	0.9799805904929428
1102	5	1.1731398134283495
1102	6	1.0801985345921565
1102	7	1.0785532936127993
1102	8	1.1386484141541757
1102	9	1.295056946593654
1102	10	1.2429843587955602
1102	11	1.3123863703046368
1102	12	1.3844059606371957
1102	13	1.3487440480516995
1102	14	1.5462138910861398
1102	15	1.5718747422732804
1102	16	1.4738698197867564
1102	17	1.6219852474120415
1102	18	0.9306844590428796
1102	19	1.3466454787271545
1102	20	1.5120209788903622
1102	21	1.3562795945558364
1102	22	1.248291444072187
1102	23	1.4847156815923412
1102	24	1.5116807399592034
1102	25	1.6649489845150498
1103	1	0.8918328939785773
1103	2	1.0314416052988307
1103	3	0.7765478246663733
1103	4	0.6796500921831523
1103	5	0.8682008378264285
1103	6	0.9273127284783058
1103	7	0.9184043074532582
1103	8	1.0746861402324157
1103	9	1.08270766666449
1103	10	1.0929290262961828
1103	11	1.1254235273426687
1103	12	1.322214206960659
1103	13	1.2740137135392757
1103	14	1.4193606714291391
1103	15	1.4244805183456322
1103	16	1.3567429070588446
1103	17	1.3732851046580876
1103	18	1.3776852040934173
1103	19	1.2934465957342098
1103	20	1.2364328634192034
1103	21	1.3608128184514499
1103	22	1.3993042368502557
1103	23	1.5170835623411492
1103	24	1.4297190748894977
1103	25	1.3926012551530818
1104	1	0.9243075917920649
1104	2	0.9921350868303662
1104	3	0.8797770014549542
1104	4	0.7746821301788529
1104	5	0.8268343667188702
1104	6	0.8291761711090047
1104	7	0.750091182290376
1104	8	0.8001303678512102
1104	9	0.8665187246538153
1104	10	0.9540356453820493
1104	11	1.0380659287526228
1104	12	0.9424054394584758
1104	13	0.8122183436633392
1104	14	1.1225590033078916
1104	15	1.216519203449117
1104	16	1.1842305356647316
1104	17	1.1357144592654933
1104	18	0.7486049915785244
1104	19	1.1731521011345976
1104	20	1.157160791404955
1104	21	1.172160476541272
1104	22	0.9861757755268883
1104	23	0.954691174080402
1104	24	1.1587068418220312
1104	25	1.1383778649052445
1105	1	0.6717326807017479
1105	2	0.6631984160480044
1105	3	0.7147431895443113
1105	4	0.5885511341523681
1105	5	0.5325649128439665
1105	6	0.6480971201410242
1105	7	0.7233939178375053
1105	8	0.6601929284980272
1105	9	0.8971830684714917
1105	10	0.6018445167164117
1105	11	0.8843015255068281
1105	12	0.8189582042927128
1105	13	0.8513308838720661
1105	14	1.0074349789281527
1105	15	1.003610173142654
1105	16	0.9148920545854864
1105	17	0.7184851965384382
1105	18	0.6683453420259937
1105	19	0.854937522549271
1105	20	1.0354010081230953
1105	21	0.9099937829415836
1105	22	0.9643231215654708
1105	23	1.0518192013917158
1105	24	0.9452166898630452
1105	25	0.818737795374183
1106	1	0.2432960142074272
1106	2	0.5182613558877769
1106	3	-1.490963654209441
1106	4	-0.10337353545296679
1106	5	0.21518546585160436
1106	6	0.24038989548359524
1106	7	0.5102435666142142
1106	8	0.47037010106351573
1106	9	0.7812342533097905
1106	10	0.48581954461930366
1106	11	0.6160583547461442
1106	12	0.7418934544167755
1106	13	0.7484145432770646
1106	14	0.7983862736525751
1106	15	0.8272330906446399
1106	16	0.7723053878442556
1106	17	0.8411996629469556
1106	18	0.8652313682338573
1106	19	0.8165950044713668
1106	20	0.8558652572314784
1106	21	0.7774162199290089
1106	22	0.7651777332754719
1106	23	0.850294325353488
1106	24	0.7623268209428176
1106	25	0.8683560617103367
1107	1	0.8708867691963
1107	2	0.9888692841210265
1107	3	0.9253796017175411
1107	4	0.6672423104255785
1107	5	0.9263462672805574
1107	6	0.8441953560235191
1107	7	0.8214513958244254
1107	8	0.8694690986826288
1107	9	0.9572784321380254
1107	10	1.00186519503346
1107	11	1.010229405269584
1107	12	1.247828831505423
1107	13	1.1071359403461614
1107	14	1.2279728699536463
1107	15	1.262531877390856
1107	16	1.2854329370286877
1107	17	1.265410189753723
1107	18	1.3749884959220395
1107	19	1.2654759345136668
1107	20	1.2391978792594145
1107	21	1.3105301680214367
1107	22	1.303389000772711
1107	23	1.5189608096461171
1107	24	1.4624344029685759
1107	25	1.4058277465082938
1108	1	0.7962983992709987
1108	2	0.7515155996102401
1108	3	0.7005537743510608
1108	4	0.6501493289050799
1108	5	0.8533428057959114
1108	6	0.7394045017512809
1108	7	0.777150171630943
1108	8	0.7079135462814534
1108	9	0.8915479074672632
1108	10	0.8658402042093343
1108	11	0.8307476072983491
1108	12	0.9478417757411108
1108	13	1.0027579159406983
1108	14	1.1373574746272297
1108	15	1.1197601684205338
1108	16	1.0747568829184004
1108	17	1.0498001174485054
1108	18	1.1693029009299352
1108	19	1.2397723325012606
1108	20	1.160392493646242
1108	21	1.2167628682025224
1108	22	1.2173620497249047
1108	23	1.2405779536272519
1108	24	1.223382421216229
1108	25	1.1695498192352929
1109	1	0.43113592627921093
1109	2	0.3026090045185903
1109	3	0.45537617919237405
1109	4	0.4130260068352243
1109	5	0.16678965610278595
1109	6	0.5117712597969586
1109	7	0.44256858226133033
1109	8	0.5057295844612781
1109	9	0.6584572790082035
1109	10	0.15741965098253985
1109	11	0.5138374890266064
1109	12	0.7781322679131151
1109	13	0.6235513469325298
1109	14	0.7982425346973798
1109	15	0.761860086557995
1109	16	0.7317721408861075
1109	17	0.8654970586655111
1109	18	0.9850309269870223
1109	19	0.9825306935417828
1109	20	1.0107264559700297
1109	21	1.007049776066708
1109	22	0.9617024381013266
1109	23	0.8767370204846685
1109	24	0.9612708818636904
1109	25	0.9148177167227018
1110	1	0.5204129762549492
1110	2	0.337300901655901
1110	3	0.45998559209873235
1110	4	0.4562089277474044
1110	5	0.5042472009839116
1110	6	0.5847940642608123
1110	7	0.4275273401458506
1110	8	0.6326097397257158
1110	9	0.4978556293235812
1110	10	0.7083657936317351
1110	11	0.5875880955283037
1110	12	0.7419847667001136
1110	13	0.6533912304842033
1110	14	0.6871314235862656
1110	15	0.6788906542435882
1110	16	0.5881525387512241
1110	17	0.7488061319309196
1110	18	0.7632939061233657
1110	19	0.8316330710284348
1110	20	0.8409059804726979
1110	21	0.858578160346184
1110	22	0.8229348495520767
1110	23	0.8629087817655972
1110	24	0.8074620511989608
1110	25	0.7782335342869899
1111	1	0.3647704794274418
1111	2	0.21373100693974578
1111	3	0.19759997893985215
1111	4	0.37968210148106607
1111	5	0.5109085201474244
1111	6	0.497669851412212
1111	7	0.3586483776040339
1111	8	0.36104680835267544
1111	9	0.4729910996005344
1111	10	0.2875654254321347
1111	11	0.3516506274941804
1111	12	0.6125772007518151
1111	13	0.4822943667676043
1111	14	0.3332438467395633
1111	15	0.34201096372235185
1111	16	0.26941503907198694
1111	17	0.5138424960166503
1111	18	0.32430055644478273
1111	19	0.20580000702147316
1111	20	0.45163128377676287
1111	21	0.5080432385608091
1111	22	0.28068106026901063
1111	23	0.41299260701127594
1111	24	0.4326768175448876
1111	25	0.4090122700506811
1112	1	0.017281559490932352
1112	2	-0.3644644314015437
1112	3	-0.013981415166835376
1112	4	-0.00394421003972667
1112	5	-0.1251080559788278
1112	6	-0.18522132998565155
1112	7	-0.11633309046197127
1112	8	-0.050738178386858124
1112	9	-0.09225375891557519
1112	10	-0.34815046540352057
1112	11	-0.272293966534356
1112	12	0.06629313922555014
1112	13	-0.07623845502580906
1112	14	-0.2975811413088181
1112	15	-0.495801712288916
1112	16	-0.5316920770703675
1112	17	-0.37719929581024303
1112	18	-0.17099819084219092
1112	19	-0.2635312963202452
1112	20	-0.3229843094171514
1112	21	-0.3063453976885455
1112	22	-0.15244716868440378
1112	23	0.08400265936744944
1112	24	-0.007821719749593115
1112	25	-0.08961651119165737
1113	1	-0.6351997188812568
1113	2	-0.5194107167213531
1113	3	-0.50868054944101
1113	4	-2.190476734036281
1113	5	-0.7334889024623621
1113	6	-0.41385692422035447
1113	7	-0.4361364263823688
1113	8	-0.3597973129950631
1113	9	-0.3734895799380027
1113	10	-0.5818999178561697
1113	11	-0.5612180989296007
1113	12	-0.2227475249117461
1113	13	-0.48254294879850335
1113	14	-0.6104597587590206
1113	15	-0.6577981680031897
1113	16	-0.8644489726242668
1113	17	-0.9158727141480654
1113	18	-0.7081496742621667
1113	19	-0.5174712965176269
1113	20	-0.29585911080075533
1113	21	-0.4116372673157187
1113	22	-0.4521184125222035
1113	23	-0.2206236507922199
1113	24	-0.15666837182174476
1113	25	-0.4179534248288519
1114	1	-0.9857061483596858
1114	2	-2.1957250785725186
1114	3	-1.5663699910272442
1114	4	-1.1260746427418553
1114	5	-1.12633987090814
1114	6	-0.593381554160203
1114	7	-0.6917238428468409
1114	8	-0.6481729829186946
1114	9	-0.90166065116005
1114	10	-1.0228840423799923
1114	11	-0.9959963012987032
1114	12	-0.9554457597114094
1114	13	-0.9963229575022936
1114	14	-1.1316797202477247
1114	15	-1.3627575403763998
1114	16	-1.658169127861747
1114	17	-1.6963702609905553
1114	18	-1.4753828822052668
1114	19	-1.820142644949449
1114	20	-1.265526995600716
1114	21	-1.4236675755849149
1114	22	-1.0993287283028934
1114	23	-0.8893490223427492
1114	24	-1.0320968546794518
1114	25	-1.7654969884947036
1115	1	-1.3431944702215795
1115	2	-1.4544791529531444
1115	3	-0.9988797071117723
1115	4	-0.8035014362671973
1115	5	-1.1173233028733947
1115	6	-0.975437458369754
1115	7	-0.8289720103103946
1115	8	-1.1532107734464034
1115	9	-2.216206954864667
1115	10	-1.8950370150511375
1115	11	-2.075675513137057
1115	12	-2.882739476732734
1115	13	-2.9484456507195844
1115	14	-2.4826546321172884
1115	15	-2.848319889488039
1115	16	-2.4034906538931082
1115	17	-2.3927798629112402
1115	18	-2.738252629922613
1115	19	-3.2104497323301047
1115	20	-3.275702878946756
1115	21	-2.62732897712786
1115	22	-2.5684418216951244
1115	23	-2.5192411685854097
1115	24	-3.0470298981013038
1115	25	-3.139270115111465
1116	1	-3.006610180545792
1116	2	-3.041786526117498
1116	3	-3.174944673738466
1116	4	-2.794313426453524
1116	5	-3.5316636046373375
1116	6	-4.1761456024757315
1116	7	-3.5021546315235064
1116	8	-4.109859059268061
1116	9	-4.736750053535815
1116	10	-3.816252762318477
1116	11	-4.679295041805329
1116	12	-6.916160120527476
1116	13	-5.98655294675503
1116	14	-5.494692126493305
1116	15	-6.370841008737319
1116	16	-6.173455141011946
1116	17	-5.83366333630611
1116	18	-6.120043462353117
1116	19	-6.615577414832323
1116	20	-8.229214473471067
1116	21	-7.127737783488559
1116	22	-6.340663139962455
1116	23	-6.875242168018524
1116	24	-6.59683493336809
1116	25	-6.0579445749630345
1201	1	0.8539812479233042
1201	2	0.9890013809807847
1201	3	1.0487448003360278
1201	4	0.8876027105816036
1201	5	0.48836310099984814
1201	6	0.9694910475053788
1201	7	1.1037842451548627
1201	8	1.140921452578219
1201	9	1.1180871021069976
1201	10	1.2137397516283315
1201	11	0.9623155845563716
1201	12	1.44038548461954
1201	13	1.4264242071625617
1201	14	1.432977920163374
1201	15	1.373965794087404
1201	16	1.2687272049173615
1201	17	1.2031885765469497
1201	18	1.509636645074778
1201	19	1.5504762597654889
1201	20	1.5239044210951391
1201	21	1.3405645319920794
1201	22	1.3007866926354694
1201	23	1.3822007546092863
1201	24	1.201601697242097
1201	25	1.3873593139003546
1202	1	1.098532881374286
1202	2	1.1962799328586797
1202	3	1.1156631228713678
1202	4	1.0802521113787122
1202	5	1.1808924633798128
1202	6	1.2248329087853875
1202	7	1.2874892060185772
1202	8	1.219759504974924
1202	9	1.5525619049023263
1202	10	1.6425563633916838
1202	11	1.6112936983608037
1202	12	1.5751380143169058
1202	13	1.4676106139587972
1202	14	1.5705403076728714
1202	15	1.6020082612276454
1202	16	1.618890009296372
1202	17	1.6342144292293455
1202	18	1.6127255527603737
1202	19	1.754457639124638
1202	20	1.7318841478323534
1202	21	1.8725275218055657
1202	22	1.7434119097575405
1202	23	1.9098617811695746
1202	24	1.8214245550084698
1202	25	1.9559973172378804
1203	1	1.0427491342584574
1203	2	1.1051777930846498
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2211	15	-0.09371601355958603
2211	16	0.087727441164553
2211	17	-0.1277286998440914
2211	18	-0.22923683422397545
2211	19	-0.1002715410071815
2211	20	-0.10267515524817838
2211	21	-0.08308511865333656
2211	22	-0.10440406141128733
2211	23	-0.21312467527222623
2211	24	-0.015199037837675178
2211	25	-0.2652477198929546
2212	1	-0.11869005415399414
2212	2	-0.05135266821491413
2212	3	-0.0618602410555944
2212	4	-0.12565709017179216
2212	5	-0.38692084786341496
2212	6	-0.13634755943798638
2212	7	-0.319394640980569
2212	8	-0.1759473772607265
2212	9	-0.17090475766272561
2212	10	-0.41808486498238295
2212	11	-0.18002436184060144
2212	12	0.09608357738638773
2212	13	0.18148035995918121
2212	14	0.1374606638978335
2212	15	-0.08899268092229562
2212	16	-0.07347716013509853
2212	17	-0.22778636130745156
2212	18	-0.23415909014672565
2212	19	0.033558512157540576
2212	20	-0.10189523575890291
2212	21	-0.059043192785502185
2212	22	-0.004038438159220448
2212	23	-0.3842641739504984
2212	24	-0.4933229846959861
2212	25	-0.3117383075291116
2213	1	-0.3360408092453895
2213	2	0.08806719501255955
2213	3	-0.04230281287804161
2213	4	-0.13222974287525346
2213	5	0.08976920896878227
2213	6	0.04339215712443689
2213	7	-0.39165023735310456
2213	8	-0.46323141273784074
2213	9	-0.19531041987043096
2213	10	-0.2612188745011963
2213	11	-0.034896611455444315
2213	12	-0.2678251270935945
2213	13	-0.21039288887780572
2213	14	-0.40608525315879973
2213	15	-0.6052743271107488
2213	16	-1.0567958506505093
2213	17	-0.869360853203375
2213	18	-0.4293271902928666
2213	19	-0.4056253107196639
2213	20	-0.421745886684775
2213	21	-0.32501266193499845
2213	22	-0.41629272575268317
2213	23	-0.5593575970033626
2213	24	-0.37464688629931686
2213	25	-0.4692221337549469
2214	1	-1.276489843536556
2214	2	-0.607088352768784
2214	3	-0.7833409274904568
2214	4	-0.8665276602277103
2214	5	-1.1828639676712953
2214	6	-1.723446904258644
2214	7	-1.2187555073976029
2214	8	-1.0113079146722868
2214	9	-0.8453972599785757
2214	10	-1.2010127170485774
2214	11	-1.3741822224420588
2214	12	-1.0189771874973985
2214	13	-1.6012306721067162
2214	14	-1.0986013023952879
2214	15	-1.2345233977631977
2214	16	-1.1769821471299409
2214	17	-1.2243955823600317
2214	18	-1.2728695504982
2214	19	-1.2047230275341363
2214	20	-1.4627535624963954
2214	21	-1.6526553627518865
2214	22	-2.143334501285125
2214	23	-2.1592574657446377
2214	24	-1.892246435639784
2214	25	-2.365970140426697
2215	1	-2.2052525771081726
2215	2	-1.5837946915554681
2215	3	-1.6422950196490937
2215	4	-1.7171827592829771
2215	5	-2.2565539035278555
2215	6	-2.0903409577275873
2215	7	-2.502140373403723
2215	8	-2.211705528986407
2215	9	-2.321940158919162
2215	10	-2.398196193012204
2215	11	-2.047890913690793
2215	12	-2.3289057562466304
2215	13	-2.3370909819457957
2215	14	-2.5778267582852834
2215	15	-2.5124670565651
2215	16	-2.502786887421344
2215	17	-2.6303555101249785
2215	18	-2.4275313371904446
2215	19	-2.6877413595903192
2215	20	-2.5417700297307277
2215	21	-2.3018893389486124
2215	22	-2.4941139217970942
2215	23	-3.0942003689721744
2215	24	-2.804849523313205
2215	25	-3.110611189013877
2216	1	-3.156715416788277
2216	2	-2.276689466435286
2216	3	-2.215438205955259
2216	4	-2.773161227941525
2216	5	-3.8053913719307175
2216	6	-3.7268464777588584
2216	7	-3.6395203670609177
2216	8	-3.761422146630359
2216	9	-6.061491160930213
2216	10	-4.170815416523443
2216	11	-3.9361940741661883
2216	12	-4.5721621164859485
2216	13	-4.554209451079924
2216	14	-4.577795364869136
2216	15	-4.433994203320374
2216	16	-3.9086242727318457
2216	17	-4.360303466520456
2216	18	-4.737688135949028
2216	19	-4.704757259086502
2216	20	-4.163559652162778
2216	21	-4.7852696644408965
2216	22	-4.335705682882242
2216	23	-5.886833897194055
2216	24	-5.103877556431524
2216	25	-4.983563636938879
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73909.0
3	3.0
4	1161.0
5	10127.0
6	12829.0
7	10255.0
8	8246.0
9	7030.0
10	5826.0
11	5232.0
12	4685.0
13	4281.0
14	4231.0
15	4296.0
16	4235.0
17	4503.0
18	4960.0
19	6076.0
20	7906.0
21	10719.0
22	13289.0
23	15991.0
24	18377.0
25	20907.0
26	23508.0
27	25439.0
28	28888.0
29	33777.0
30	42595.0
31	54320.0
32	68497.0
33	88422.0
34	116264.0
35	152991.0
36	208891.0
37	329587.0
38	1054886.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37532595048772	19.58069085407076	13.663120110742685	26.380863084698834
2	13.393981591536114	20.78150095388874	40.518611388985086	25.305906065590055
3	17.609408585394494	25.432215437141714	29.658317465360227	27.300058512103558
4	11.512908838178427	17.120389607308674	37.153706629267106	34.21299492524579
5	12.414817031044281	39.3450210841169	34.962999244784065	13.277162640054755
6	31.643438865239325	37.414924933587436	17.74590350253601	13.19573269863723
7	28.01632914509377	32.15341606809765	21.520762284277765	18.30949250253081
8	25.636820261426074	37.362173634770244	19.8634096419421	17.137596461861584
9	26.906766284119666	14.822412640016665	19.734663567897904	38.53615750796576
10	15.19812996592039	29.694836247316914	33.548422651515885	21.55861113524681
11	34.453715286112754	22.657175748057202	24.30646612054882	18.58264284528122
12	23.53935233861639	25.31653916376808	31.26491426071706	19.87919423689847
13	29.054140993040576	22.285189956686562	25.931086117021874	22.729582933250985
14	20.825203116374315	22.50115696019615	27.69760699218092	28.976032931248618
15	22.82478442070655	31.657744849983192	23.344611603401656	22.1728591259086
16	21.72790995419219	28.453640498739	27.90472682587884	21.913722721189973
17	20.963261879334524	29.040638389582984	28.033605038664934	21.962494692417557
18	20.892328464949124	27.473070958211082	30.63427046010071	21.00033011673909
19	23.14380983130131	26.68930886089284	28.349638152432334	21.817243155373514
20	23.392442074358563	26.96389505735771	28.956367448014987	20.687295420268736
21	22.7923384908091	26.423184275555723	27.9409151256024	22.84356210803278
22	22.958052335972127	27.88543228461158	28.073842749232746	21.08267263018355
23	21.814743767839275	27.595683232822932	28.81145766280051	21.778115336537283
24	22.733630890754398	26.621350877453974	29.35549641576124	21.289521816030387
25	22.134187112179898	27.245280621629913	29.038505688664767	21.582026577525422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	173.0
1	173.0
2	501.5
3	830.0
4	830.0
5	830.0
6	2276.0
7	3722.0
8	3722.0
9	3722.0
10	4987.5
11	6253.0
12	6253.0
13	6253.0
14	8786.5
15	11320.0
16	11320.0
17	11320.0
18	20906.5
19	30493.0
20	30493.0
21	30493.0
22	48588.5
23	66684.0
24	66684.0
25	66684.0
26	96328.0
27	125972.0
28	125972.0
29	125972.0
30	160339.0
31	194706.0
32	194706.0
33	194706.0
34	218049.0
35	241392.0
36	241392.0
37	241392.0
38	256864.0
39	272336.0
40	272336.0
41	272336.0
42	286455.0
43	300574.0
44	300574.0
45	300574.0
46	312453.5
47	324333.0
48	324333.0
49	324333.0
50	311632.5
51	298932.0
52	298932.0
53	298932.0
54	265593.0
55	232254.0
56	232254.0
57	232254.0
58	199528.5
59	166803.0
60	166803.0
61	166803.0
62	137440.0
63	108077.0
64	108077.0
65	108077.0
66	83295.0
67	58513.0
68	58513.0
69	58513.0
70	42813.0
71	27113.0
72	27113.0
73	27113.0
74	19094.5
75	11076.0
76	11076.0
77	11076.0
78	7486.5
79	3897.0
80	3897.0
81	3897.0
82	2561.0
83	1225.0
84	1225.0
85	1225.0
86	803.0
87	381.0
88	381.0
89	381.0
90	224.5
91	68.0
92	68.0
93	68.0
94	39.5
95	11.0
96	11.0
97	11.0
98	6.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08439415730282866
2	0.040206840068046056
3	0.01905804219225383
4	0.004905234488301618
5	0.017409561749463943
6	0.06191853370479093
7	0.07104548640023738
8	0.05275137416927642
9	0.022515830438105792
10	0.04804717388131503
11	0.005226889208845988
12	2.412410404082763E-4
13	0.006071232850274954
14	7.23723121224829E-4
15	0.014112600863884167
16	0.009126952695446455
17	0.005267096048914033
18	0.005628957609526448
19	0.0016886872828579345
20	0.0011257915219052895
21	2.010342003402303E-4
22	6.433094410887368E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2487139.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.343831413153126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13662871743605	46.369269217996646
2	12.936573363949325	15.354116575481921
3	4.103298263191198	7.305163211061075
4	1.7572398890110696	4.171253909037633
5	0.9215524359796359	2.734422619957805
6	0.5105905819051744	1.8180240848234652
7	0.33727972819525626	1.4010829930365378
8	0.22554311055709653	1.0707673863438796
9	0.1632825370364278	0.8720830215541533
>10	0.8228416190253743	8.538551950362779
>50	0.04958423684982083	2.020776854418399
>100	0.030499336301907112	3.504367592242514
>500	0.002780445373765723	1.13602058599515
>1k	0.0020344722247001026	2.272000927525913
>5k	2.0344722247001026E-4	0.9076735261610033
>10k+	6.781574082333676E-5	0.524425544001243
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	13031	0.5239353329267082	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9631	0.3872320766953516	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7573	0.3044863998353128	No Hit
GTCCTACAGTGGACATTTCTAAATT	5350	0.21510659436404642	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4932	0.19830013521560316	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4651	0.1870020131564822	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4071	0.1636820459170155	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3936	0.15825412250782928	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3476	0.1397589760765281	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2627	0.10562336885875699	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.020684006804606E-5	0.0
5	0.0	0.0	0.0	4.020684006804606E-5	0.0
6	4.824820808165527E-4	0.0	0.0	4.020684006804606E-5	0.0
7	4.824820808165527E-4	0.0	0.0	4.020684006804606E-5	4.020684006804606E-5
8	5.628957609526448E-4	0.0	0.0	4.020684006804606E-5	4.020684006804606E-5
9	5.628957609526448E-4	0.0	0.0	4.020684006804606E-5	4.020684006804606E-5
10	5.628957609526448E-4	0.0	0.0	8.041368013609212E-5	4.020684006804606E-5
11	5.628957609526448E-4	0.0	0.0	8.041368013609212E-5	4.020684006804606E-5
12	5.628957609526448E-4	0.0	0.0	8.041368013609212E-5	8.041368013609212E-5
13	5.628957609526448E-4	0.0	0.0	8.041368013609212E-5	8.041368013609212E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGACCG	125	0.0	14.454649	5
GGTATCA	2215	0.0	13.870958	1
CGCTAGA	65	5.4732347E-5	13.149181	10
TAGGACC	950	0.0	13.113289	4
TCCAACG	175	1.8189894E-12	11.391145	18
CGTCCAA	60	0.0058928756	11.079402	10
ACCGCTA	95	1.3611849E-5	10.99986	8
GGCGTGC	165	8.0035534E-11	10.939255	8
AGGCGTG	200	0.0	10.93168	7
CGGTTTC	140	8.451025E-9	10.849583	13
GTATCAA	4930	0.0	10.804851	1
GATATAC	700	0.0	10.735115	1
GGCGAGG	1075	0.0	10.684504	19
AGGACCT	1870	0.0	10.577564	5
GCCGGTT	135	5.7485522E-8	10.548418	11
GTCTAGC	100	2.3664055E-5	10.463341	1
GTCCTAA	1405	0.0	10.426105	1
GTAGGAC	2645	0.0	10.391329	3
GGACCGC	110	5.976024E-6	10.374149	6
CTACACT	415	0.0	10.311655	4
>>END_MODULE