FastQCFastQC Report
Thu 26 May 2016
SRR1512921_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512921_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58580.36055354632540665No Hit
GTATCAACGCAGAGTACTTTTTTTT53280.32793262117134975No Hit
GTCCTAAAGTGTGTATTTCTCATTT51490.31691536531743236No Hit
CTGTAGGACGTGGAATATGGCAAGA47160.2902646849557217No Hit
CTTTAGGACGTGAAATATGGCGAGG41830.2574591130555097No Hit
GTCCTACAGTGTGCATTTCTCATTT37780.23253180232457946No Hit
TATCAACGCAGAGTACTTTTTTTTT31060.19117093118585063No Hit
GGTATCAACGCAGAGTACTTTTTTT30600.18833968107814003No Hit
GTACTGGTTCACTATCGGTCAGTCA27840.17135218043187644No Hit
CTGTAGGACCTGGAATATGGCGAGA26580.16359701709336477No Hit
CTGAAGGACCTGGAATATGGCGAGA23350.14371671742400555No Hit
GTCCTTCAGTGTGCATTTCTCATTT19170.11798927079307009No Hit
TTTCTAAATTTTCCACCTTTTTCAG17010.10469470506990726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10800.013.5441014
TATGTCG1251.03682396E-1012.91870116
TAGGACT2350.012.5298834
TGCCGGC550.003071565412.08932214
AGGACCT17600.012.088955
TACGACT550.00307227412.088954
GGACCTG17100.011.7203226
GGTATCA16200.011.6411371
GTCCTAT2200.011.2563061
TTGGACC855.3317635E-511.1746594
ATAGGAC2900.011.1361263
ACACCGA600.00588294511.0815376
TGTAGGA28550.011.0454952
CTGTAGG28250.010.9237511
CGCCCTA3150.010.85605116
GCGCCAC1053.471374E-610.85571813
AATCCCG1408.365532E-910.85571719
GGCGAGG12100.010.83328819
GTAGGAC29400.010.7261523
CTACACT2150.010.6029324
Produced by FastQC (version 0.11.5)