FastQCFastQC Report
Thu 26 May 2016
SRR1512921_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512921_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1624724
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70010.4309039566104766No Hit
GTCCTACAGTGGACATTTCTAAATT54420.3349491975252412No Hit
TATCAACGCAGAGTACTTTTTTTTT50130.30854471282507057No Hit
CTGTAGGACGTGGAATATGGCAAGA48200.29666577215576306No Hit
GTCCTAAAGTGTGTATTTCTCATTT46490.28614090762492583No Hit
CTTTAGGACGTGAAATATGGCGAGG45550.2803553095787346No Hit
GGTATCAACGCAGAGTACTTTTTTT41080.25284294440163374No Hit
GTCCTACAGTGTGCATTTCTCATTT35760.2200989214168068No Hit
CTGTAGGACCTGGAATATGGCGAGA26130.16082731590103921No Hit
ACGCAGAGTACTTTTTTTTTTTTTT23880.14697880993941123No Hit
GTACTGGTTCACTATCGGTCAGTCA23810.14654796753171614No Hit
CTGAAGGACCTGGAATATGGCGAGA23650.14556318488555595No Hit
TTTCTAAATTTTCCACCTTTTTCAG19610.12069742307001066No Hit
ATTTAGAAATGTCCACTGTAGGACG16800.10340217784682197No Hit
GTCCTTCAGTGTGCATTTCTCATTT16380.10081712340065142No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGC250.00598601319.0252933
CCGCTAC400.00523605714.2676511
TCCAACG1351.8189894E-1213.35808918
TAGGACC10150.012.8373564
GCCGGTT905.451766E-712.6577611
CCAACGT604.1261854E-412.65425419
TAGGACT2350.012.5462764
GGTATCA16700.012.1887321
ATAGTAC1302.561137E-911.7078743
GGTCTAG907.3589053E-611.6254931
CCGGTTT907.5274074E-611.60187512
GGCGAGG13050.011.27246819
CCACCTT5450.011.1470913
AGGACCT17200.011.1144665
AAATGTC5750.011.0761027
GATCTCG600.00591888511.07247319
GGACCTG16550.010.9763116
TGTAGGA29350.010.7928852
CTGCGCA803.7749956E-410.6862359
GTAGGAC29900.010.6579833
Produced by FastQC (version 0.11.5)