FastQCFastQC Report
Thu 26 May 2016
SRR1512920_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512920_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994791
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106700.534893129154884No Hit
GTCCTACAGTGGACATTTCTAAATT80580.4039520932268092No Hit
CTGTAGGACGTGGAATATGGCAAGA68200.34189045368662685No Hit
GGTATCAACGCAGAGTACTTTTTTT64990.32579854230342925No Hit
GTCCTAAAGTGTGTATTTCTCATTT64930.32549775891308913No Hit
TATCAACGCAGAGTACTTTTTTTTT64380.3227405778349712No Hit
CTTTAGGACGTGAAATATGGCGAGG54740.27441471312032184No Hit
GTCCTACAGTGTGCATTTCTCATTT43410.21761678291109193No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31400.15740997427800707No Hit
CTGAAGGACCTGGAATATGGCGAGA27580.13826009842635142No Hit
CTGTAGGACCTGGAATATGGCGAGA26690.13379847813630602No Hit
GTACTGGTTCACTATCGGTCAGTCA24720.12392275682013805No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23960.1201128338758296No Hit
TTTCTAAATTTTCCACCTTTTTCAG23800.11931074483492254No Hit
GTCCTTCAGTGTGCATTTCTCATTT21720.108883587303131No Hit
GAATATGGCAAGAAAACTGAAAATC21130.10592588396478629No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20960.10507366435882255No Hit
ATTTAGAAATGTCCACTGTAGGACG20890.10472275040342573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10800.013.98409754
GGTATCA21800.012.9779231
CTACACG604.0979017E-412.6648444
CACCTTT8950.011.88685514
GGCGAGG14300.011.8228819
TTTAGAA9400.011.62066652
ACCTTTT9600.011.57674815
TTCCACC9100.011.37691111
GTCCTAC35050.011.3603961
AGGACCT19900.011.2647095
GCGCCAC1354.7384674E-911.2579213
ATTTAGA8450.011.16050151
ACAGCGT600.005882947211.08173758
AATCCCG1201.2792043E-711.08118219
GTGTAAC2150.011.0766081
TAGAAAT9350.010.9716834
GGACCTG19450.010.8904636
GTTGAAC700.001493515110.855583
AGGACGA1752.0008883E-1110.8555795
CAGGACT2450.010.8555794
Produced by FastQC (version 0.11.5)