##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512920_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994791
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.03194119083152	31.0	30.0	34.0	23.0	34.0
2	29.07020384591669	31.0	30.0	34.0	22.0	34.0
3	29.226307417669318	31.0	30.0	34.0	23.0	34.0
4	32.63124357388819	35.0	33.0	37.0	25.0	37.0
5	32.82591760239544	35.0	33.0	37.0	26.0	37.0
6	32.70512449675179	37.0	33.0	37.0	25.0	37.0
7	32.599233704182545	37.0	33.0	37.0	25.0	37.0
8	32.52223466017242	37.0	33.0	37.0	23.0	37.0
9	33.78593045587232	38.0	34.0	39.0	22.0	39.0
10	33.65864293552558	38.0	34.0	39.0	19.0	39.0
11	33.728322917037424	39.0	34.0	39.0	21.0	39.0
12	33.63541594081786	38.0	34.0	39.0	19.0	39.0
13	33.610010773058434	39.0	34.0	39.0	18.0	39.0
14	34.45973989255015	39.0	34.0	41.0	17.0	41.0
15	34.66511228494614	39.0	34.0	41.0	18.0	41.0
16	34.61759903669106	39.0	34.0	41.0	18.0	41.0
17	34.409280972292336	39.0	34.0	41.0	17.0	41.0
18	34.412487323233364	39.0	34.0	41.0	17.0	41.0
19	34.53587017386784	39.0	34.0	41.0	18.0	41.0
20	34.523620268990584	39.0	34.0	41.0	18.0	41.0
21	34.42112732612088	39.0	34.0	41.0	18.0	41.0
22	34.44825197226176	39.0	34.0	41.0	18.0	41.0
23	34.12203935149096	39.0	34.0	41.0	10.0	41.0
24	34.1985270637375	39.0	34.0	41.0	2.0	41.0
25	33.928004988993834	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7893252559719599
1101	2	0.3881974205911298
1101	3	0.8013234308277184
1101	4	0.9262188615092839
1101	5	0.8856909420850982
1101	6	0.8256971447995198
1101	7	0.6870127047080103
1101	8	0.9709888577999521
1101	9	1.027476988238135
1101	10	0.977770156896824
1101	11	1.017683436622491
1101	12	0.9023105554061175
1101	13	1.241640081233541
1101	14	1.4220230841402994
1101	15	1.2993997053496642
1101	16	1.2074878195885361
1101	17	0.6163004652942661
1101	18	0.9448063017777883
1101	19	0.41730470840959555
1101	20	-0.16030634213804262
1101	21	0.923463311167815
1101	22	0.9580242185089887
1101	23	0.4782568259821858
1101	24	0.9926322585561991
1101	25	0.6507270415720114
1102	1	0.9950895537996978
1102	2	0.9821763773867893
1102	3	0.869740794465077
1102	4	1.0140802769999553
1102	5	0.7810957000977652
1102	6	0.8063276883353936
1102	7	0.7594724965204662
1102	8	0.7852554141303685
1102	9	0.9915431496999645
1102	10	1.0161047716697453
1102	11	0.7928775036107041
1102	12	1.1123158335279442
1102	13	1.0412359158889828
1102	14	1.2006489817336714
1102	15	1.1619119917304346
1102	16	1.0036890106577658
1102	17	1.2921890330638988
1102	18	1.2096587805416164
1102	19	1.0405687746485341
1102	20	1.2680114178398938
1102	21	1.369989872051434
1102	22	1.1501453074113073
1102	23	1.2159407011263283
1102	24	0.40799676162086485
1102	25	0.8669136655119729
1103	1	0.7207904093124391
1103	2	0.8711786452970465
1103	3	1.0383855569409164
1103	4	1.0417223415616093
1103	5	0.8437906118875915
1103	6	0.5327674805764957
1103	7	0.5062090890065107
1103	8	0.6183570655320381
1103	9	0.758255099653276
1103	10	0.9311903399067916
1103	11	0.7834439214065867
1103	12	0.8817508236344622
1103	13	0.9978182412857635
1103	14	1.0811296234996917
1103	15	0.9714240687342155
1103	16	1.0027027368580619
1103	17	0.7598608162401419
1103	18	1.0669887572433794
1103	19	1.001667519495058
1103	20	1.1564756507578409
1103	21	0.97164636252392
1103	22	0.9974894606725115
1103	23	0.5198924117701509
1103	24	0.6361801052872949
1103	25	0.9107159688742286
1104	1	1.0896751488570793
1104	2	0.9698693007254171
1104	3	0.9845768533991439
1104	4	1.2173267176587572
1104	5	1.0219048664191774
1104	6	0.9585411543021607
1104	7	1.124589222998182
1104	8	0.8100627966676797
1104	9	1.0990142433688845
1104	10	1.274299926426096
1104	11	0.9496152966293039
1104	12	1.1440709243583314
1104	13	1.1813237212691945
1104	14	1.4500743568641425
1104	15	1.2769405415578419
1104	16	1.1388689838419381
1104	17	1.3324122465585546
1104	18	1.201966633495033
1104	19	0.8751734268317293
1104	20	1.1276794390799196
1104	21	1.1105862963827917
1104	22	0.9411437582024078
1104	23	1.301985818038439
1104	24	1.1788570788374173
1104	25	0.820666067165476
1105	1	0.6955838342272571
1105	2	0.8414442633418702
1105	3	0.885276575461468
1105	4	0.9678194639954896
1105	5	0.7371438337183918
1105	6	0.8935529826430582
1105	7	0.7949035686464327
1105	8	0.9238692879251502
1105	9	0.5731499689254704
1105	10	0.7106616712561546
1105	11	0.8255033330032759
1105	12	0.9672654629194497
1105	13	0.8853269791229792
1105	14	1.1449903966935082
1105	15	0.9855466108487647
1105	16	0.9490191535757972
1105	17	1.3227015308589216
1105	18	1.0847536245869378
1105	19	0.9239598682012868
1105	20	0.9185739442043186
1105	21	0.9903902948639711
1105	22	0.910659771800546
1105	23	1.189353558809131
1105	24	1.2741430275618413
1105	25	1.4611517011151633
1106	1	0.7781117036032015
1106	2	0.8620757692341456
1106	3	0.7276437488944012
1106	4	0.8773989245953615
1106	5	0.7177674764735542
1106	6	0.8374423006820351
1106	7	0.7503513290828536
1106	8	0.8667092647787342
1106	9	1.0929577835178605
1106	10	0.802664067113426
1106	11	0.6732519562620993
1106	12	0.949202397237336
1106	13	0.9183290824198664
1106	14	1.1232320257287896
1106	15	0.9413506120042356
1106	16	1.0997994222698964
1106	17	1.1744315594596983
1106	18	1.110451870239828
1106	19	1.1097181764085775
1106	20	1.1679119917283103
1106	21	1.1915989291791789
1106	22	1.071129603472194
1106	23	1.3500125856813128
1106	24	1.0191097430501017
1106	25	1.2853090672226415
1107	1	0.8611410797858845
1107	2	0.8533621295860954
1107	3	0.8784794775449747
1107	4	0.8857402054090855
1107	5	0.8083840539814702
1107	6	0.8694075802088648
1107	7	0.6987794795315239
1107	8	0.8807771607228148
1107	9	0.7649911889070111
1107	10	0.8809681402298395
1107	11	0.8467035263600877
1107	12	0.8627745797875903
1107	13	0.6055820889230077
1107	14	1.0589937345971308
1107	15	0.8802083652922974
1107	16	1.0015446064189035
1107	17	1.2939606579083929
1107	18	1.1348559199416286
1107	19	1.008049718916368
1107	20	1.2272124765497807
1107	21	0.9628294729519311
1107	22	0.9894015202474264
1107	23	1.2898416529440766
1107	24	1.1807792112161906
1107	25	1.3598756602631212
1108	1	0.8650594171716932
1108	2	0.8059920740142736
1108	3	0.8723932242319918
1108	4	0.9545185032769865
1108	5	0.8312754748665583
1108	6	0.903754670297559
1108	7	0.8677891300544331
1108	8	0.8423937751075954
1108	9	1.0264421204475838
1108	10	1.1769349773621371
1108	11	0.7562576716605349
1108	12	0.8836332531723272
1108	13	0.9086093975862042
1108	14	1.0319702980549366
1108	15	1.0436250595792202
1108	16	0.8872052667601764
1108	17	1.087644908330823
1108	18	1.2123975407947754
1108	19	1.0286452102855108
1108	20	1.1027730194356806
1108	21	1.0295885594995582
1108	22	1.1007207447558045
1108	23	1.3130939097153558
1108	24	1.2861167977462173
1108	25	1.4091026863682288
1109	1	0.7836525126773637
1109	2	0.7600400900015423
1109	3	0.8474531687533187
1109	4	0.8490569730315087
1109	5	0.7593437907234843
1109	6	0.8194062660834049
1109	7	0.8416894634837391
1109	8	0.7700240199629675
1109	9	0.9183343627209695
1109	10	0.9382189820431535
1109	11	0.8277835983432382
1109	12	0.8587384753501723
1109	13	0.906099777051665
1109	14	1.0860687773448632
1109	15	0.8805537097323111
1109	16	0.583403027180232
1109	17	0.9529938016984545
1109	18	0.9688451564811231
1109	19	0.9023584734655614
1109	20	0.8523759692169577
1109	21	0.7746397503406186
1109	22	0.794715573041259
1109	23	1.0792901531404198
1109	24	0.8479985835898702
1109	25	0.37405719973790497
1110	1	0.777893466716101
1110	2	0.6648916078465632
1110	3	0.658052004598904
1110	4	0.5674771241370209
1110	5	0.5205864298850003
1110	6	0.6440393043659398
1110	7	0.6544880635355881
1110	8	0.5971376762473071
1110	9	0.7812892841436323
1110	10	0.7391083009572625
1110	11	0.6145507551152107
1110	12	0.6400513588236265
1110	13	0.6882760544734907
1110	14	0.726729462751571
1110	15	0.7476612551318667
1110	16	0.780151913023964
1110	17	0.7733800836880107
1110	18	0.24145388702196868
1110	19	0.6928230241612141
1110	20	0.729824952587947
1110	21	0.7896453674165329
1110	22	0.842171377147281
1110	23	1.0198047019842917
1110	24	0.853753000092965
1110	25	1.0612331376661288
1111	1	0.3721319902893043
1111	2	0.36895178154383146
1111	3	0.42972151452446283
1111	4	0.3558153685538983
1111	5	0.4501273728130357
1111	6	0.35589569394410603
1111	7	0.2823377843792585
1111	8	0.5358900025500333
1111	9	0.6476051960543074
1111	10	0.6097395044312108
1111	11	0.29619777744771625
1111	12	0.18564301515117165
1111	13	0.46938957804668746
1111	14	0.4166999962503297
1111	15	0.42895700575220985
1111	16	0.5313290222051066
1111	17	0.10866544348949958
1111	18	0.4548510644413213
1111	19	0.5292599038138235
1111	20	0.6927537677244189
1111	21	0.569715931932663
1111	22	0.30471712776762416
1111	23	0.5632158372200209
1111	24	0.43984093433613936
1111	25	0.6031476848125337
1112	1	0.14129566347792988
1112	2	0.22367013984404593
1112	3	0.23194512733400074
1112	4	0.3460862146254371
1112	5	0.18927900839848633
1112	6	-0.07271654237506198
1112	7	-0.01808400941468591
1112	8	0.09732429252252217
1112	9	0.061378029285734215
1112	10	0.03148916049968875
1112	11	0.07734612657642259
1112	12	0.03519064966661034
1112	13	0.004139272329780397
1112	14	0.06680680609441936
1112	15	-0.16944973148410725
1112	16	0.18885672779973106
1112	17	0.35007785559589166
1112	18	-0.009258630606062468
1112	19	0.1076922448410329
1112	20	0.023472489063458113
1112	21	-0.14225126082185113
1112	22	-0.12328109525285669
1112	23	0.08380099848569245
1112	24	0.13962855662290252
1112	25	0.0620745229354327
1113	1	-0.03290730206126824
1113	2	0.15924018352587055
1113	3	0.1469196602352092
1113	4	0.3113682680583878
1113	5	0.09637543722926978
1113	6	-0.11652514067962016
1113	7	-0.1573034551685879
1113	8	-0.06022576471536922
1113	9	-0.2741336356273294
1113	10	-0.09399404310672566
1113	11	-0.21131577163868798
1113	12	-0.2575751832074573
1113	13	-0.16975295834228632
1113	14	0.12653682653834863
1113	15	-0.12199307554230643
1113	16	-0.18703134478157324
1113	17	-0.0934590532587194
1113	18	-0.2560086250910416
1113	19	-0.5822362056627952
1113	20	-0.33686056720899415
1113	21	-0.10849297562386084
1113	22	-0.53890289778208
1113	23	-3.8906368454990847
1113	24	-1.5966367870946456
1113	25	-0.6885427572748029
1114	1	-0.8161733176523498
1114	2	-1.0306653173216453
1114	3	-0.9388334895274006
1114	4	-0.7877517910895371
1114	5	-0.7862502608984698
1114	6	-1.4134063725064614
1114	7	-1.2873466767228479
1114	8	-1.1437316546622824
1114	9	-1.4973801159392153
1114	10	-1.0913514599123886
1114	11	-1.3028236974307248
1114	12	-2.1508971995976154
1114	13	-1.3283190929419675
1114	14	-3.4235421026151442
1114	15	-2.0608429334698286
1114	16	-1.9045917638564802
1114	17	-1.6997300184229758
1114	18	-1.8094636640838004
1114	19	-1.764738779330422
1114	20	-1.280254234706696
1114	21	-1.3386314140965112
1114	22	-1.4850929411140612
1114	23	-5.5994244585660375
1114	24	-2.8676150016444133
1114	25	-2.6704579675375477
1115	1	-1.7374009875937944
1115	2	-1.5404064333060887
1115	3	-1.3019900771027082
1115	4	-1.3505289244950767
1115	5	-1.3356625166024436
1115	6	-1.4469627183781704
1115	7	-1.8633499130930957
1115	8	-2.116166178334641
1115	9	-3.2718117668208926
1115	10	-3.8022401559163406
1115	11	-2.7922955084922485
1115	12	-3.1605976049120237
1115	13	-2.3491353034698967
1115	14	-3.1572958568302347
1115	15	-2.870366157754898
1115	16	-3.0353267834820947
1115	17	-2.827227131549993
1115	18	-5.278604208070526
1115	19	-3.37297104010403
1115	20	-3.682287207789006
1115	21	-3.112140801874606
1115	22	-2.7715511384251066
1115	23	-3.403694214122787
1115	24	-3.104969985885031
1115	25	-3.3708960553138496
1116	1	-2.069004962234935
1116	2	-1.8380579977941807
1116	3	-2.2790260824330772
1116	4	-2.113412836895783
1116	5	-2.1902721591160805
1116	6	-4.460007223140483
1116	7	-2.9772717734609273
1116	8	-3.3008237846448836
1116	9	-4.418655774356928
1116	10	-3.750602145518638
1116	11	-3.391035171398819
1116	12	-3.1811046235984115
1116	13	-4.391821973273938
1116	14	-7.647183529326398
1116	15	-5.3842328763942255
1116	16	-4.359661240248922
1116	17	-5.421304466239686
1116	18	-3.9948949444185935
1116	19	-3.7360143564670203
1116	20	-4.404623886070297
1116	21	-5.271292823500303
1116	22	-4.9226632425098735
1116	23	-3.9337177761199946
1116	24	-4.928466383450555
1116	25	-4.869817147183454
1201	1	0.5407050249328762
1201	2	0.5920838601580876
1201	3	0.918684081146715
1201	4	0.8837036513653587
1201	5	0.4595977259109745
1201	6	0.861460733917454
1201	7	1.0187791151587362
1201	8	0.8932132453881394
1201	9	1.3783748912575007
1201	10	1.215685124018151
1201	11	1.3329058054290641
1201	12	1.1269810814403058
1201	13	1.4054513701852613
1201	14	1.4110658356907066
1201	15	1.3178991693098396
1201	16	1.3338968936241642
1201	17	1.454075170338271
1201	18	1.1937545038150716
1201	19	1.3246623438537952
1201	20	1.361384888643805
1201	21	1.4673064965562475
1201	22	1.2392747143765561
1201	23	1.6274727909175581
1201	24	1.5232076481935692
1201	25	1.5443012907419131
1202	1	1.0623625129717489
1202	2	1.1124404403209525
1202	3	1.1714796095277507
1202	4	1.3368749099740143
1202	5	1.1233785233718834
1202	6	1.323788938520039
1202	7	1.2976186440110808
1202	8	1.207198968690733
1202	9	1.47516375128113
1202	10	1.494527061959154
1202	11	1.3702107623260886
1202	12	1.613843006932889
1202	13	1.4605820501045486
1202	14	1.7685445886237687
1202	15	1.4747958864228252
1202	16	1.3120600379842031
1202	17	1.5837870739957438
1202	18	1.6179676407069365
1202	19	1.5535894978309202
1202	20	1.5215154787697855
1202	21	1.682028349749885
1202	22	1.5555814850983296
1202	23	1.6745363089491505
1202	24	1.7384452653683553
1202	25	2.029004234779663
1203	1	1.039909308170099
1203	2	0.9817580729345607
1203	3	0.9995787165112198
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2211	19	0.2602841475860629
2211	20	0.0974447051233227
2211	21	0.27911792562402127
2211	22	0.3208523793043483
2211	23	0.4451360289055657
2211	24	0.15792943724781594
2211	25	0.5703568202056104
2212	1	-0.22172138121196738
2212	2	-0.07875496145871352
2212	3	-0.35335339005568756
2212	4	-0.23703688971582437
2212	5	-0.27412199105464197
2212	6	-0.3410747259032263
2212	7	-0.30403705658766
2212	8	-0.184661047068289
2212	9	-0.04969998842406653
2212	10	-0.3692251902252792
2212	11	-0.4526985329848543
2212	12	-0.023759815637632187
2212	13	0.042350773975442735
2212	14	-0.08486329891506017
2212	15	-0.2892912448839198
2212	16	-0.3926992856829301
2212	17	-0.18324295345706787
2212	18	0.034362037723767
2212	19	-0.16288306692408838
2212	20	-0.11201961013817652
2212	21	0.06713243956625092
2212	22	0.08575322827653054
2212	23	0.07325639065513911
2212	24	0.042982632809419385
2212	25	0.28210522298047636
2213	1	-0.1868115619638111
2213	2	-0.17907754092340866
2213	3	-0.41178485731957437
2213	4	-0.18722583870081877
2213	5	-0.0889474114630886
2213	6	0.059951502509143495
2213	7	-0.15816087733374928
2213	8	0.029194073339425586
2213	9	-0.4062301946456017
2213	10	-0.09577124181359409
2213	11	-0.1749227882282156
2213	12	-0.23170128414572844
2213	13	-0.11327780769955353
2213	14	-0.3098897688503399
2213	15	-0.2008325635990218
2213	16	-0.1553993654586847
2213	17	-0.2945496412020958
2213	18	-0.2625977011387377
2213	19	-0.11473248592730556
2213	20	0.09550834186951818
2213	21	-0.10786646260233113
2213	22	-0.2766244550064201
2213	23	-0.18457389192666795
2213	24	0.017979217333902398
2213	25	0.13202282463356596
2214	1	-2.6439552938423354
2214	2	-1.2832341910856897
2214	3	-1.615706047384954
2214	4	-3.4220177000352194
2214	5	-1.7934910696763708
2214	6	-1.495178556626115
2214	7	-1.2583437283284624
2214	8	-1.1870190272642382
2214	9	-1.0268635844931069
2214	10	-1.2000537914306335
2214	11	-1.1630923007536467
2214	12	-1.1258270280225062
2214	13	-1.4688284897278265
2214	14	-1.4454169392335388
2214	15	-1.6799930797805374
2214	16	-1.566515756224348
2214	17	-3.462940942955594
2214	18	-3.4146230109904145
2214	19	-2.2709587138140677
2214	20	-2.2212490630021975
2214	21	-2.1029092106219167
2214	22	-3.035914694487179
2214	23	-1.79991231934563
2214	24	-2.960985576742388
2214	25	-2.7560306916944732
2215	1	-1.8994993216314384
2215	2	-3.3513370487533862
2215	3	-2.0752556147500663
2215	4	-3.5553945827084874
2215	5	-2.320011649238733
2215	6	-2.1509785070785874
2215	7	-2.144418467618184
2215	8	-1.6569676396679256
2215	9	-3.937070718774713
2215	10	-2.4505171670293215
2215	11	-1.8693938652496271
2215	12	-1.990729727737932
2215	13	-1.9582762097449375
2215	14	-1.7732966080961035
2215	15	-1.7869623175140106
2215	16	-1.8869429896197545
2215	17	-2.2108168192142372
2215	18	-1.916800321784308
2215	19	-2.0389894370298975
2215	20	-1.8875533391787442
2215	21	-2.393261157392999
2215	22	-2.359078913299804
2215	23	-2.401256538139517
2215	24	-2.3495503448265325
2215	25	-3.200990917776153
2216	1	-3.743574540095679
2216	2	-3.5540950685993415
2216	3	-4.823961769758068
2216	4	-5.624758625241839
2216	5	-5.191220065939746
2216	6	-4.109961965934993
2216	7	-5.779602937065221
2216	8	-3.541853693759222
2216	9	-4.329763261629438
2216	10	-3.254904744693828
2216	11	-3.5875132470511666
2216	12	-3.9500648077886993
2216	13	-3.548591252370109
2216	14	-4.522536600949795
2216	15	-4.033361937136746
2216	16	-4.227177778622128
2216	17	-3.9677647187758502
2216	18	-3.5639662478399394
2216	19	-4.428432686863875
2216	20	-4.9228934045632045
2216	21	-4.717947029410006
2216	22	-4.284421334688627
2216	23	-4.381335845089872
2216	24	-4.807398864423007
2216	25	-4.741685376752848
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96793.0
3	0.0
4	875.0
5	5415.0
6	7104.0
7	6694.0
8	5916.0
9	5250.0
10	4568.0
11	4199.0
12	4012.0
13	3773.0
14	3804.0
15	3780.0
16	3898.0
17	4212.0
18	4744.0
19	5764.0
20	7608.0
21	9834.0
22	12411.0
23	15004.0
24	17456.0
25	19658.0
26	21502.0
27	23758.0
28	26499.0
29	30871.0
30	37700.0
31	46353.0
32	58207.0
33	73847.0
34	95680.0
35	125558.0
36	171756.0
37	265683.0
38	764605.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.01139651585331	19.310243182010247	12.73405715159539	23.944303150541053
2	14.046330439665395	20.499201588300323	43.60265418620916	21.851813785825122
3	18.950720586215162	26.52051061201448	29.40907217693944	25.119696624830922
4	11.487868282056489	17.083524477768336	39.51122174509229	31.91738549508289
5	11.882919071550905	40.10003248849867	35.53128722640473	12.485761213545699
6	29.97219115906627	37.80472203450544	19.264307405339316	12.958779401088973
7	27.579299082651655	32.4804025596452	22.361726732037457	17.57857162566569
8	24.48130697845037	38.38079958890959	20.94508641078229	16.192807021857742
9	27.62972064337165	14.75245423535273	20.790974454479183	36.82685066679644
10	15.663095529589327	29.39911169804959	34.377596567212706	20.56019620514838
11	33.27710626965209	22.71085023448291	26.020052089474284	17.99199140639072
12	24.173066038303904	25.43047733357954	32.16406811366729	18.232388514449266
13	29.403613409078023	23.178708269720804	26.024753322192044	21.392924999009125
14	20.075611236823224	24.325444410438063	28.423954763708615	27.174989589030098
15	22.000256879297837	33.802204947535415	23.68637847351484	20.51115969965191
16	20.093612213237964	28.97618603232326	30.37214090900881	20.55806084542997
17	20.16999723010594	30.215680410426042	28.999486164579878	20.614836194888138
18	19.89773260438124	27.367417447178994	32.992927090572884	19.741922857866886
19	22.782789071237865	27.077242048568372	29.005746777504427	21.13422210268934
20	23.418840643703263	27.443692021146347	30.028229254057266	19.109238081093125
21	22.108386375620164	27.16342041349153	28.32064281883444	22.407550392053864
22	22.667126987958216	29.860282786475988	28.295458023866786	19.177132201699006
23	20.639826026569686	29.32505012674691	29.862143919460017	20.172979927223388
24	22.478187635754967	27.03172721210665	30.786278719592016	19.70380643254637
25	21.299530670958898	28.413623687820426	30.229677209594502	20.057168431626174
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	349.0
1	349.0
2	806.0
3	1263.0
4	1263.0
5	1263.0
6	2589.5
7	3916.0
8	3916.0
9	3916.0
10	4749.5
11	5583.0
12	5583.0
13	5583.0
14	7750.0
15	9917.0
16	9917.0
17	9917.0
18	17891.5
19	25866.0
20	25866.0
21	25866.0
22	41958.5
23	58051.0
24	58051.0
25	58051.0
26	86853.0
27	115655.0
28	115655.0
29	115655.0
30	150237.5
31	184820.0
32	184820.0
33	184820.0
34	204638.5
35	224457.0
36	224457.0
37	224457.0
38	232743.5
39	241030.0
40	241030.0
41	241030.0
42	248282.0
43	255534.0
44	255534.0
45	255534.0
46	262454.5
47	269375.0
48	269375.0
49	269375.0
50	249692.5
51	230010.0
52	230010.0
53	230010.0
54	192180.0
55	154350.0
56	154350.0
57	154350.0
58	127469.0
59	100588.0
60	100588.0
61	100588.0
62	81403.5
63	62219.0
64	62219.0
65	62219.0
66	46274.0
67	30329.0
68	30329.0
69	30329.0
70	21947.5
71	13566.0
72	13566.0
73	13566.0
74	9354.0
75	5142.0
76	5142.0
77	5142.0
78	3522.5
79	1903.0
80	1903.0
81	1903.0
82	1244.0
83	585.0
84	585.0
85	585.0
86	391.5
87	198.0
88	198.0
89	198.0
90	129.0
91	60.0
92	60.0
93	60.0
94	36.5
95	13.0
96	13.0
97	13.0
98	12.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3416398008613433
2	0.010326896401678172
3	0.008271543234353874
4	0.011329507702811974
5	0.011981205048548946
6	0.07720107018730282
7	0.07750185357764297
8	0.10236661384576129
9	0.080409426350931
10	0.08898175297562501
11	0.08431961042535283
12	0.09459637626197431
13	0.080409426350931
14	0.08527209116142995
15	0.08206373499780177
16	0.083968696469956
17	0.09700264338469544
18	0.09905799655201973
19	0.09264128422476339
20	0.09138802009834614
21	0.07870498713900353
22	0.0992585188122465
23	0.09239063139947995
24	0.08873110015034157
25	0.08687626924324403
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1994791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.47797577902427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68095626076807	43.30785190408079
2	13.766864101386956	15.550492345425024
3	4.435180558017951	7.514700603940115
4	1.9463015291624715	4.396926824908638
5	1.0382975882235574	2.932047301955469
6	0.5814666636770095	1.9704036088476116
7	0.3480378422525444	1.3759530977446153
8	0.223601748230126	1.0102859296550832
9	0.16118895275176748	0.8193263192424549
>10	0.7198218270137123	7.064602235202984
>50	0.04944785851377272	1.9315512720117518
>100	0.03987630406197295	4.597233812637498
>500	0.005196084245327187	2.004664890426907
>1k	0.0031355680790767482	2.9712250469595958
>5k	5.375259564131569E-4	2.0128616524725684
>10k+	8.958765940219282E-5	0.5398731544890228
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10670	0.534893129154884	No Hit
GTCCTACAGTGGACATTTCTAAATT	8058	0.4039520932268092	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6820	0.34189045368662685	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6499	0.32579854230342925	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	6493	0.32549775891308913	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6438	0.3227405778349712	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5474	0.27441471312032184	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4341	0.21761678291109193	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3140	0.15740997427800707	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2758	0.13826009842635142	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2669	0.13379847813630602	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2472	0.12392275682013805	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2396	0.1201128338758296	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2380	0.11931074483492254	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2172	0.108883587303131	No Hit
GAATATGGCAAGAAAACTGAAAATC	2113	0.10592588396478629	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2096	0.10507366435882255	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2089	0.10472275040342573	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.013056505669015E-5	0.0
4	0.0	0.0	0.0	2.005222602267606E-4	0.0
5	0.0	0.0	0.0	2.005222602267606E-4	0.0
6	0.0	0.0	0.0	2.005222602267606E-4	0.0
7	0.0	0.0	0.0	2.005222602267606E-4	0.0
8	0.0	0.0	0.0	2.005222602267606E-4	0.0
9	0.0	0.0	0.0	5.514362156235917E-4	5.013056505669015E-5
10	1.002611301133803E-4	0.0	0.0	6.015667806802818E-4	5.013056505669015E-5
11	1.002611301133803E-4	0.0	0.0	6.015667806802818E-4	5.013056505669015E-5
12	1.002611301133803E-4	0.0	0.0	6.015667806802818E-4	5.013056505669015E-5
13	1.5039169517007045E-4	0.0	0.0	7.018279107936621E-4	5.013056505669015E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1080	0.0	13.9840975	4
GGTATCA	2180	0.0	12.977923	1
CTACACG	60	4.0979017E-4	12.664844	4
CACCTTT	895	0.0	11.886855	14
GGCGAGG	1430	0.0	11.82288	19
TTTAGAA	940	0.0	11.6206665	2
ACCTTTT	960	0.0	11.576748	15
TTCCACC	910	0.0	11.376911	11
GTCCTAC	3505	0.0	11.360396	1
AGGACCT	1990	0.0	11.264709	5
GCGCCAC	135	4.7384674E-9	11.25792	13
ATTTAGA	845	0.0	11.1605015	1
ACAGCGT	60	0.0058829472	11.0817375	8
AATCCCG	120	1.2792043E-7	11.081182	19
GTGTAAC	215	0.0	11.076608	1
TAGAAAT	935	0.0	10.971683	4
GGACCTG	1945	0.0	10.890463	6
GTTGAAC	70	0.0014935151	10.85558	3
AGGACGA	175	2.0008883E-11	10.855579	5
CAGGACT	245	0.0	10.855579	4
>>END_MODULE