FastQCFastQC Report
Thu 26 May 2016
SRR1512920_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512920_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994791
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT127310.6382122237367223No Hit
TATCAACGCAGAGTACTTTTTTTTT93910.4707761364473772No Hit
GGTATCAACGCAGAGTACTTTTTTT77090.38645652602202435No Hit
GTCCTACAGTGGACATTTCTAAATT75710.37953850804420114No Hit
CTGTAGGACGTGGAATATGGCAAGA65920.3304606848537015No Hit
GTCCTAAAGTGTGTATTTCTCATTT60560.30359070198331556No Hit
CTTTAGGACGTGAAATATGGCGAGG56520.28333795370041276No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45110.22613897897072927No Hit
GTCCTACAGTGTGCATTTCTCATTT42380.21245333471025285No Hit
CTGAAGGACCTGGAATATGGCGAGA29830.1495394755641067No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26980.13525226452295003No Hit
CTGTAGGACCTGGAATATGGCGAGA26540.13304651966045564No Hit
TTTCTAAATTTTCCACCTTTTTCAG24480.12271962325877749No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23120.11590186641106763No Hit
GTACTGGTTCACTATCGGTCAGTCA21860.10958541521392466No Hit
ATTTAGAAATGTCCACTGTAGGACG21230.10642718961535319No Hit
GTCCTTCAGTGTGCATTTCTCATTT20540.10296818062644156No Hit
GAATATGGCAAGAAAACTGAAAATC20380.10216609158553452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19350.014.010361
GGCGAGG13800.012.58662519
TAGGACC11500.012.4057864
GCGTGCG853.9498427E-612.2927389
CCCGTAC2250.012.23416916
GCCGGTT701.0992686E-412.20464811
CGAAGAC550.00308832312.08076414
GGACGAG1459.640644E-1111.8059966
CTAATAC1950.011.7074413
ATAATAC2450.011.6477113
AAGTCGT2700.011.6185517
CCCCGTA2300.011.55551215
AAGGCGT1251.8031642E-811.4124616
GACGAGG1251.8153514E-811.4073057
CGAGGCA752.0765388E-411.3987219
TCCTATA4100.011.3683552
CCGTACA2350.011.30965217
GGCGTGC1104.964586E-711.2282678
GTATCAA44350.011.2174331
ATAGACG600.0058203311.097683
Produced by FastQC (version 0.11.5)