##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512920_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994791 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.289962206567004 31.0 30.0 34.0 26.0 34.0 2 30.30479082771077 31.0 30.0 34.0 26.0 34.0 3 30.46742741470159 31.0 31.0 34.0 26.0 34.0 4 34.03797490564175 37.0 35.0 37.0 30.0 37.0 5 33.633624274422736 37.0 35.0 37.0 28.0 37.0 6 33.47900206086753 37.0 35.0 37.0 28.0 37.0 7 33.30506704712423 37.0 35.0 37.0 27.0 37.0 8 33.24640024944969 37.0 35.0 37.0 27.0 37.0 9 34.54128377358831 39.0 35.0 39.0 27.0 39.0 10 34.476262926792835 39.0 35.0 39.0 27.0 39.0 11 34.431780572501076 39.0 35.0 39.0 26.0 39.0 12 34.30810546067232 39.0 35.0 39.0 25.0 39.0 13 34.29172630115135 39.0 35.0 39.0 25.0 39.0 14 35.37789171898209 40.0 36.0 41.0 25.0 41.0 15 35.39221001097358 40.0 36.0 41.0 25.0 41.0 16 35.40053218607864 40.0 36.0 41.0 25.0 41.0 17 35.41732943451219 40.0 36.0 41.0 26.0 41.0 18 35.35651554473627 40.0 36.0 41.0 25.0 41.0 19 35.37996010609633 40.0 36.0 41.0 25.0 41.0 20 35.364925949635825 40.0 36.0 41.0 25.0 41.0 21 35.33287497286683 40.0 36.0 41.0 25.0 41.0 22 35.30048711870066 40.0 36.0 41.0 25.0 41.0 23 35.14233771858806 40.0 35.0 41.0 25.0 41.0 24 35.11264237707108 40.0 36.0 41.0 24.0 41.0 25 35.05660292231116 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7691923843423254 1101 2 0.6644697249631193 1101 3 0.5927248435209869 1101 4 0.6063069918881325 1101 5 0.7210526206597905 1101 6 0.8190976436897159 1101 7 0.8000692838670034 1101 8 0.6849802982160469 1101 9 1.0868740935866015 1101 10 0.6127760906599775 1101 11 0.8922272104059061 1101 12 1.0262257092522162 1101 13 1.080058600187428 1101 14 1.1237708944814528 1101 15 0.8586399860676224 1101 16 1.0491188441671753 1101 17 1.0941524551154416 1101 18 1.03997797300174 1101 19 0.8805062043505885 1101 20 1.0781652210121777 1101 21 1.1983166485820576 1101 22 0.9688050525731597 1101 23 1.1209690839322874 1101 24 1.184662355315048 1101 25 1.1699306811346517 1102 1 0.7500172743224276 1102 2 0.8833387946711824 1102 3 0.5254859542219847 1102 4 0.6283224818309776 1102 5 0.8234019216489799 1102 6 0.756069298880611 1102 7 0.7523304483010236 1102 8 0.9876709007796691 1102 9 0.9675257872395022 1102 10 0.9050527784608988 1102 11 0.9280999559843863 1102 12 1.0411250868234134 1102 13 0.9960374084563597 1102 14 1.0478025738372665 1102 15 1.1502344691520108 1102 16 1.0220132703310796 1102 17 1.0894366762759446 1102 18 0.5596238419057613 1102 19 0.925239974624013 1102 20 1.1462770091754422 1102 21 1.0597159806102283 1102 22 0.8300235405866019 1102 23 1.020877234209017 1102 24 0.9075554257022205 1102 25 0.9875259183629268 1103 1 0.8417586545247744 1103 2 1.0438551471183466 1103 3 0.8159450194596012 1103 4 0.6803589999356703 1103 5 0.9220225165471163 1103 6 0.8943605124670384 1103 7 0.9448942852252173 1103 8 0.9745216436178197 1103 9 1.1095118965012034 1103 10 0.8888793935836361 1103 11 1.0420901167821057 1103 12 1.1894259776185336 1103 13 1.1485320037225861 1103 14 1.3174787520437832 1103 15 1.4039792987152495 1103 16 1.1744236882999814 1103 17 1.1968937670576594 1103 18 1.2467074611309243 1103 19 1.2569460215648363 1103 20 1.0693383233251126 1103 21 1.3341205057442522 1103 22 1.102759256102111 1103 23 1.253221205121413 1103 24 1.2726870688176177 1103 25 1.143106661141701 1104 1 0.7496360896163914 1104 2 0.8489623470341918 1104 3 0.7448235788189663 1104 4 0.5989934091033859 1104 5 0.7200068885679656 1104 6 0.6383833199805622 1104 7 0.6578674145686065 1104 8 0.7668723494081249 1104 9 0.9198697884005611 1104 10 0.8726605164049559 1104 11 1.109991615148303 1104 12 1.0893620389996101 1104 13 0.9538167195984286 1104 14 1.1668449543199344 1104 15 1.236900619696982 1104 16 1.1427436641052182 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2216 18 -2.8192039942843437 2216 19 -3.119562103971404 2216 20 -2.4015777860034078 2216 21 -3.0961661843092116 2216 22 -2.5062110185605633 2216 23 -3.5958956964356403 2216 24 -2.9105689446681 2216 25 -3.0732175939254596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 55003.0 3 0.0 4 939.0 5 8183.0 6 10992.0 7 8528.0 8 6922.0 9 5601.0 10 4673.0 11 4159.0 12 3746.0 13 3321.0 14 3131.0 15 3317.0 16 3323.0 17 3411.0 18 3850.0 19 4787.0 20 6520.0 21 8685.0 22 10794.0 23 12917.0 24 15065.0 25 17400.0 26 18766.0 27 20274.0 28 22644.0 29 26965.0 30 33672.0 31 42804.0 32 54145.0 33 70026.0 34 91106.0 35 120569.0 36 166997.0 37 260030.0 38 861526.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.000411432401904 20.192650713458907 13.755891134986456 26.051046719152733 2 12.598956732117072 21.0259954351388 42.51830683871576 23.856740994028367 3 16.99604743083004 26.459900952617804 30.12431313894562 26.419738477606536 4 10.764744986408834 17.710678114462585 38.658119255338455 32.86645764379013 5 11.493663879248189 40.08136627962363 35.68729637120833 12.737673469919846 6 29.967941563454488 38.44479212178092 18.68829779282856 12.898968521936027 7 27.035037875462248 32.63761618628537 22.446786468082415 17.880559470169967 8 24.456254601848563 38.60221169606834 20.609410140819072 16.332123561264023 9 26.902607743405433 14.592392176368655 20.725312479943522 37.779687600282394 10 15.0533201324502 30.032861772910703 34.24315103876706 20.670667055872034 11 32.869482824312676 22.807919064712134 25.94270760222191 18.379890508753284 12 23.671523497519782 25.79235356191269 32.096291078988465 18.439831861579066 13 29.077911784302135 23.129887064535197 26.18947710383475 21.602724047327914 14 19.853136539504646 24.16384179091608 28.44346588943732 27.53955578014195 15 21.755571142716615 33.70135857316977 23.723714840520447 20.819355443593167 16 20.04225395892723 28.945064839873137 30.126501173665375 20.88618002753426 17 19.84495898627261 30.257161735761507 29.124458940651664 20.77342033731422 18 19.656657223227953 27.377483038658273 33.12345871499381 19.842401023119965 19 22.463237376783237 27.01632329150869 29.311239414124124 21.209199917583945 20 23.19757325284281 27.374096950574227 30.20835996548974 19.219969831093227 21 21.681591386357155 26.854469319812775 28.826758118277173 22.637181175552904 22 22.40146181167394 29.521677774797784 28.749865273701175 19.3269951398271 23 20.26773732185477 29.027401868165637 30.297409603311827 20.407451206667766 24 22.25882310477639 26.995960980373386 30.99878633901998 19.74642957583025 25 21.002622332487295 28.284044076842214 30.451090315817865 20.26224327485263 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 132.0 1 132.0 2 500.5 3 869.0 4 869.0 5 869.0 6 2244.5 7 3620.0 8 3620.0 9 3620.0 10 4495.0 11 5370.0 12 5370.0 13 5370.0 14 7366.5 15 9363.0 16 9363.0 17 9363.0 18 17717.5 19 26072.0 20 26072.0 21 26072.0 22 43365.5 23 60659.0 24 60659.0 25 60659.0 26 90578.5 27 120498.0 28 120498.0 29 120498.0 30 153231.0 31 185964.0 32 185964.0 33 185964.0 34 205159.5 35 224355.0 36 224355.0 37 224355.0 38 232729.5 39 241104.0 40 241104.0 41 241104.0 42 248736.5 43 256369.0 44 256369.0 45 256369.0 46 262562.5 47 268756.0 48 268756.0 49 268756.0 50 248776.0 51 228796.0 52 228796.0 53 228796.0 54 191237.0 55 153678.0 56 153678.0 57 153678.0 58 126319.0 59 98960.0 60 98960.0 61 98960.0 62 79833.0 63 60706.0 64 60706.0 65 60706.0 66 44996.0 67 29286.0 68 29286.0 69 29286.0 70 21161.5 71 13037.0 72 13037.0 73 13037.0 74 8933.5 75 4830.0 76 4830.0 77 4830.0 78 3272.0 79 1714.0 80 1714.0 81 1714.0 82 1112.0 83 510.0 84 510.0 85 510.0 86 313.5 87 117.0 88 117.0 89 117.0 90 71.5 91 26.0 92 26.0 93 26.0 94 13.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08792901110943452 2 0.04331280820898029 3 0.019651181502222538 4 0.005664753851405987 5 0.017696089465011625 6 0.06236242293052254 7 0.07760211470775635 8 0.05148409031322078 9 0.021205229018979935 10 0.0493786065808398 11 0.005564492721292606 12 3.007833903401409E-4 13 0.005614623286349297 14 6.51697345736972E-4 15 0.01363551369541972 16 0.009274154535487678 17 0.004662142550272184 18 0.005414101026122537 19 0.001704439211927465 20 0.0011028724312471831 21 1.002611301133803E-4 22 3.007833903401409E-4 23 0.0 24 0.0 25 1.002611301133803E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1994791.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.58293566736642 #Duplication Level Percentage of deduplicated Percentage of total 1 76.15227811531847 42.32767175407141 2 13.921732898467317 15.476215681475352 3 4.71709518903531 7.865699952869798 4 1.96668821519718 4.3725721817229015 5 1.0385539234316197 2.8862937956595354 6 0.580780711255725 1.936889814634456 7 0.3792193258594127 1.475468637514606 8 0.24194874869496824 1.075857738680978 9 0.16608880215828167 0.8308532884890347 >10 0.7348038666044255 7.168926034183926 >50 0.05075805160265463 1.9570780841983417 >100 0.04100280286944785 4.700466489987054 >500 0.005429609703040468 2.0826276577844323 >1k 0.0029862853366722556 3.0416272695056725 >5k 5.429609703040465E-4 2.161395799605236 >10k+ 9.04934950506744E-5 0.6403558196172829 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12731 0.6382122237367223 No Hit TATCAACGCAGAGTACTTTTTTTTT 9391 0.4707761364473772 No Hit GGTATCAACGCAGAGTACTTTTTTT 7709 0.38645652602202435 No Hit GTCCTACAGTGGACATTTCTAAATT 7571 0.37953850804420114 No Hit CTGTAGGACGTGGAATATGGCAAGA 6592 0.3304606848537015 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6056 0.30359070198331556 No Hit CTTTAGGACGTGAAATATGGCGAGG 5652 0.28333795370041276 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4511 0.22613897897072927 No Hit GTCCTACAGTGTGCATTTCTCATTT 4238 0.21245333471025285 No Hit CTGAAGGACCTGGAATATGGCGAGA 2983 0.1495394755641067 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2698 0.13525226452295003 No Hit CTGTAGGACCTGGAATATGGCGAGA 2654 0.13304651966045564 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2448 0.12271962325877749 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2312 0.11590186641106763 No Hit GTACTGGTTCACTATCGGTCAGTCA 2186 0.10958541521392466 No Hit ATTTAGAAATGTCCACTGTAGGACG 2123 0.10642718961535319 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2054 0.10296818062644156 No Hit GAATATGGCAAGAAAACTGAAAATC 2038 0.10216609158553452 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.5039169517007045E-4 0.0 4 0.0 0.0 0.0 2.005222602267606E-4 0.0 5 0.0 0.0 0.0 2.005222602267606E-4 0.0 6 0.0 0.0 0.0 2.005222602267606E-4 0.0 7 0.0 0.0 0.0 2.005222602267606E-4 0.0 8 5.013056505669015E-5 0.0 0.0 2.005222602267606E-4 0.0 9 5.013056505669015E-5 0.0 0.0 4.5117508551021133E-4 0.0 10 2.5065282528345076E-4 0.0 0.0 4.5117508551021133E-4 0.0 11 2.5065282528345076E-4 0.0 0.0 5.013056505669015E-4 0.0 12 2.5065282528345076E-4 0.0 0.0 5.013056505669015E-4 0.0 13 2.5065282528345076E-4 0.0 0.0 5.514362156235917E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1935 0.0 14.01036 1 GGCGAGG 1380 0.0 12.586625 19 TAGGACC 1150 0.0 12.405786 4 GCGTGCG 85 3.9498427E-6 12.292738 9 CCCGTAC 225 0.0 12.234169 16 GCCGGTT 70 1.0992686E-4 12.204648 11 CGAAGAC 55 0.003088323 12.080764 14 GGACGAG 145 9.640644E-11 11.805996 6 CTAATAC 195 0.0 11.707441 3 ATAATAC 245 0.0 11.647711 3 AAGTCGT 270 0.0 11.618551 7 CCCCGTA 230 0.0 11.555512 15 AAGGCGT 125 1.8031642E-8 11.412461 6 GACGAGG 125 1.8153514E-8 11.407305 7 CGAGGCA 75 2.0765388E-4 11.398721 9 TCCTATA 410 0.0 11.368355 2 CCGTACA 235 0.0 11.309652 17 GGCGTGC 110 4.964586E-7 11.228267 8 GTATCAA 4435 0.0 11.217433 1 ATAGACG 60 0.00582033 11.09768 3 >>END_MODULE