FastQCFastQC Report
Thu 26 May 2016
SRR1512919_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512919_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372551
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67380.2839981100511643No Hit
GTCCTACAGTGGACATTTCTAAATT57390.24189153362772814No Hit
GTCCTAAAGTGTGTATTTCTCATTT51450.21685519088946878No Hit
CTGTAGGACGTGGAATATGGCAAGA47530.20033289063122353No Hit
CTTTAGGACGTGAAATATGGCGAGG40410.17032299832543113No Hit
TATCAACGCAGAGTACTTTTTTTTT39150.16501225895670948No Hit
GGTATCAACGCAGAGTACTTTTTTT37340.15738333970481563No Hit
GTCCTACAGTGTGCATTTCTCATTT28300.11928089217049496No Hit
GTACTGGTTCACTATCGGTCAGTCA27090.1141808964275162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8850.011.9135084
ATAGGAC2650.011.8284563
TAGAAAT7400.011.4240014
GATATAC6000.011.1147691
TCCAACG2650.011.11134518
CCGATCA600.005884540311.0814759
ATTTAGA7600.011.0311991
GTCCCGC4650.011.03017612
TGTAGAA9200.010.9440482
GGCGAGG11200.010.9401319
CTAAGAC2700.010.9058083
CGCCCTA4450.010.88581616
CCCGCCC4500.010.76486114
TCCCGCC4600.010.7373313
CGGTTTC1951.8189894E-1210.71615113
TTTAGAA8600.010.7135472
GTCTTAC2050.010.6887671
AAATGTC7300.010.6698947
GTCCTAT2600.010.6262071
GTCCTAA13900.010.6235711
Produced by FastQC (version 0.11.5)