##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512919_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2372551
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.854437270263105	31.0	30.0	34.0	22.0	34.0
2	28.882727073095584	31.0	30.0	34.0	16.0	34.0
3	29.049564371851226	31.0	30.0	34.0	22.0	34.0
4	32.45277762206165	35.0	33.0	37.0	23.0	37.0
5	32.62865919425968	35.0	33.0	37.0	25.0	37.0
6	32.511567928360655	36.0	33.0	37.0	23.0	37.0
7	32.417951816420384	36.0	33.0	37.0	23.0	37.0
8	32.35420397706941	36.0	33.0	37.0	22.0	37.0
9	33.64229135643449	38.0	34.0	39.0	19.0	39.0
10	33.497118080917964	38.0	34.0	39.0	18.0	39.0
11	33.586367163445594	38.0	34.0	39.0	19.0	39.0
12	33.47632906521293	38.0	34.0	39.0	18.0	39.0
13	33.461678168351284	38.0	34.0	39.0	17.0	39.0
14	34.30000746032435	39.0	34.0	41.0	17.0	41.0
15	34.48461887647515	39.0	34.0	41.0	17.0	41.0
16	34.43195994522352	39.0	34.0	41.0	17.0	41.0
17	34.189242718070126	39.0	33.0	41.0	17.0	41.0
18	34.19392164804887	39.0	34.0	41.0	17.0	41.0
19	34.30568067872935	39.0	34.0	41.0	18.0	41.0
20	34.274512539456474	39.0	34.0	41.0	16.0	41.0
21	34.168596586543345	39.0	34.0	41.0	11.0	41.0
22	34.18102835302592	39.0	34.0	41.0	10.0	41.0
23	33.848882068288525	39.0	34.0	41.0	2.0	41.0
24	33.91362335309125	39.0	34.0	41.0	2.0	41.0
25	33.62453789191465	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5211996229736222
1101	2	0.12683788170993182
1101	3	0.48192943123967424
1101	4	0.483926103824885
1101	5	0.4866646929635081
1101	6	0.41443182057591343
1101	7	0.1824937157110469
1101	8	0.40846628209784086
1101	9	0.47672477985152995
1101	10	0.4726736808337648
1101	11	0.40650932754929414
1101	12	0.34432896781529365
1101	13	0.7611192013785839
1101	14	0.8069396840826428
1101	15	0.5249904663291503
1101	16	0.43093778216402967
1101	17	-0.11468537282568292
1101	18	0.35596336215206037
1101	19	-0.2665197498771761
1101	20	-0.8604172142912532
1101	21	0.2631684848295279
1101	22	0.40967585168093024
1101	23	-0.10347154745877418
1101	24	0.4339213361442873
1101	25	0.12953936153028422
1102	1	1.1106976767200756
1102	2	1.0330345637963028
1102	3	0.9877213450935045
1102	4	1.134180656845146
1102	5	1.0235217539384749
1102	6	0.9759176132909175
1102	7	0.9563991701621362
1102	8	1.1558879930975792
1102	9	1.314607015802224
1102	10	1.3597587634089408
1102	11	1.2141487621562135
1102	12	1.2746104007775187
1102	13	1.2119056803272201
1102	14	1.543232003753289
1102	15	1.4495288313132306
1102	16	1.298840052090334
1102	17	1.5577591637197443
1102	18	1.5001466557159233
1102	19	1.3319375050027134
1102	20	1.5503438602757669
1102	21	1.6667338641100216
1102	22	1.6158054470688512
1102	23	1.422269399212766
1102	24	0.8080989997966412
1102	25	1.2124632130355053
1103	1	0.8407439856277321
1103	2	0.9390547217914005
1103	3	1.1032212088908366
1103	4	1.2567630594602335
1103	5	0.8736988174089149
1103	6	0.6460347475867536
1103	7	0.47533134123268894
1103	8	0.795223324070065
1103	9	0.8874754440234227
1103	10	1.0944359630820557
1103	11	0.891620840608951
1103	12	0.980957445179591
1103	13	1.146800804271649
1103	14	1.2923466864010678
1103	15	1.0872297302508471
1103	16	1.2547103871489043
1103	17	0.716299071101929
1103	18	1.1097898047216432
1103	19	1.0058683140465732
1103	20	1.3400469384560978
1103	21	1.2074584623121467
1103	22	1.214824787847931
1103	23	0.5140699985045174
1103	24	0.7936615168219703
1103	25	1.1182109656914605
1104	1	1.0562107412478703
1104	2	1.0261681009422254
1104	3	1.0493432614452622
1104	4	1.2412573720269506
1104	5	1.0497666221343351
1104	6	0.9765395101327599
1104	7	1.101429782390575
1104	8	0.8255631471635496
1104	9	1.1849062817453841
1104	10	1.1894184386319537
1104	11	0.9932530429393722
1104	12	1.3550205109029108
1104	13	1.3007316045140414
1104	14	1.5861566014566577
1104	15	1.2699239378747649
1104	16	1.1824955410432523
1104	17	1.4609923378492482
1104	18	1.2664577872073153
1104	19	0.8153796757842855
1104	20	1.0844809287161041
1104	21	1.1976430686380226
1104	22	0.867359749100217
1104	23	1.442002723627283
1104	24	1.3535293012160068
1104	25	0.8196021252360524
1105	1	0.7354217138485168
1105	2	0.6762842694649507
1105	3	0.6835794770898644
1105	4	0.8378569382392769
1105	5	0.5736677144112363
1105	6	0.8279722552463369
1105	7	0.7415998643601398
1105	8	0.7698988480728062
1105	9	0.46817850245582093
1105	10	0.6646246255602222
1105	11	0.681651719990306
1105	12	0.8111062545823913
1105	13	0.8801003676680352
1105	14	0.9141980524063911
1105	15	0.8547622664258725
1105	16	0.7696005479726011
1105	17	1.2386936650466325
1105	18	1.001618385094865
1105	19	0.7879847728468619
1105	20	0.959605220711012
1105	21	0.8180053697564773
1105	22	0.7179778506074541
1105	23	1.0048142429978881
1105	24	1.0719209570609465
1105	25	1.2723756164230906
1106	1	0.5697970972057043
1106	2	0.636816296219628
1106	3	0.6092432326759258
1106	4	0.6790781316849674
1106	5	0.5937801658526638
1106	6	0.6703330053120524
1106	7	0.674344263694195
1106	8	0.722236231605244
1106	9	0.8645775736361188
1106	10	0.5483635629671255
1106	11	0.36793366642252323
1106	12	0.5382580202160625
1106	13	0.7356655456918091
1106	14	0.7388174699955457
1106	15	0.5972198415638772
1106	16	0.7730049058310584
1106	17	0.93127449864771
1106	18	0.7963449966554563
1106	19	0.8437018333369721
1106	20	0.8009492344972671
1106	21	0.8567897258624484
1106	22	0.7300473952521358
1106	23	0.9983631812810358
1106	24	0.6503717559043025
1106	25	0.8931883336588271
1107	1	0.7853048049205746
1107	2	0.8175337753855523
1107	3	0.847721003697778
1107	4	0.9216697837469141
1107	5	0.7692699902383708
1107	6	0.7856282590764962
1107	7	0.550189608663608
1107	8	0.7302980363908205
1107	9	0.7099518717154538
1107	10	0.8451896297077113
1107	11	0.8278492571088876
1107	12	0.7889844410465656
1107	13	0.6654548800743001
1107	14	0.9873943146609534
1107	15	0.7395017543647668
1107	16	0.783875823961175
1107	17	1.1718381811828493
1107	18	0.8938964112267911
1107	19	0.8275059751160114
1107	20	0.9073893789945657
1107	21	0.7739212398650892
1107	22	0.6909973576971424
1107	23	1.0627639109208644
1107	24	0.8897601460470099
1107	25	1.1497897857851527
1108	1	0.8582991778972442
1108	2	0.7647568580956445
1108	3	0.8207504391912579
1108	4	0.8594103279307888
1108	5	0.8196143177699327
1108	6	0.8371698183712937
1108	7	0.8107490430337307
1108	8	0.9132722909719249
1108	9	1.028962564081013
1108	10	1.0930196684126443
1108	11	0.7717989304828237
1108	12	0.9620697042367112
1108	13	0.9607520774558438
1108	14	0.9031070879698646
1108	15	1.0106905779322233
1108	16	0.836828734660692
1108	17	1.1661743932736286
1108	18	1.1441358916207207
1108	19	0.9689508912212759
1108	20	1.144478373108825
1108	21	1.0799152281753166
1108	22	1.051836611376153
1108	23	1.3734988280814093
1108	24	1.1844636635323127
1108	25	1.3738705861636973
1109	1	0.8860472334652876
1109	2	0.8458610256989374
1109	3	0.9811919264007187
1109	4	0.9554045733113625
1109	5	0.7136079337389987
1109	6	0.9562005246609147
1109	7	0.7576312082092898
1109	8	0.7299067377641748
1109	9	0.9741767887809161
1109	10	1.0345428442432407
1109	11	1.1017049683374296
1109	12	1.005063005500027
1109	13	1.0021718499685477
1109	14	1.1589853418025342
1109	15	0.962019085777392
1109	16	0.5908007708068936
1109	17	1.0054316877033358
1109	18	1.0912383508213708
1109	19	1.0120431000060677
1109	20	0.8862128513716527
1109	21	0.8697187618713116
1109	22	0.9043070515146212
1109	23	1.075490465900053
1109	24	0.9098514259744306
1109	25	0.5911164612704738
1110	1	0.6511717070940826
1110	2	0.6248375933916961
1110	3	0.5850373600126098
1110	4	0.5968543980200565
1110	5	0.5100824213266222
1110	6	0.5761888774440038
1110	7	0.5516936655525555
1110	8	0.5965577650583072
1110	9	0.7353473820961867
1110	10	0.601685277084826
1110	11	0.5243963946001884
1110	12	0.6374423371089151
1110	13	0.49841044368714194
1110	14	0.606689063493036
1110	15	0.4637499115979935
1110	16	0.758572139642979
1110	17	0.7545295085488348
1110	18	0.17099654417202714
1110	19	0.5895606102728337
1110	20	0.717926892304277
1110	21	0.8087294618086887
1110	22	0.8314310737966153
1110	23	0.9362953859917766
1110	24	0.8147125956760277
1110	25	0.9377362646945357
1111	1	0.4161228089530127
1111	2	0.4070819261290879
1111	3	0.5212769357121623
1111	4	0.40573545646132203
1111	5	0.4901448462841671
1111	6	0.3495360072370133
1111	7	0.29054916890891747
1111	8	0.4772460008629622
1111	9	0.6656058557274989
1111	10	0.5848762449443754
1111	11	0.36026481717246384
1111	12	-0.058626074132213546
1111	13	0.4613844438784511
1111	14	0.35417185388222805
1111	15	0.5176015107526126
1111	16	0.6559689816881686
1111	17	-0.07447538668540687
1111	18	0.3651470798006926
1111	19	0.4244305007813267
1111	20	0.5455785263795718
1111	21	0.42918003389201687
1111	22	0.17714099593688815
1111	23	0.5191450830539495
1111	24	0.5149140815293691
1111	25	0.6043141638651548
1112	1	0.23287880777001035
1112	2	0.25889764676269067
1112	3	0.194709424542296
1112	4	0.2298693585732039
1112	5	0.12003327148146781
1112	6	0.006684956377682738
1112	7	-0.15039896273778197
1112	8	0.02544149005415619
1112	9	-0.036637872344655875
1112	10	0.09679340694603411
1112	11	-0.001991442029421364
1112	12	0.07049189857166027
1112	13	0.03849894853419755
1112	14	0.043280877935266915
1112	15	-0.16462945568178355
1112	16	0.03643722718388176
1112	17	0.40981017461432856
1112	18	0.19630022569669592
1112	19	0.19506966454761
1112	20	0.011738679102116123
1112	21	0.08286374765620508
1112	22	-0.07998151338445325
1112	23	0.14310163119352381
1112	24	0.05866172628275024
1112	25	0.012269530925522076
1113	1	-0.3543824299892542
1113	2	-0.2452731783772144
1113	3	-0.2072146310313734
1113	4	-0.18476109726133672
1113	5	-0.27934176351558193
1113	6	-0.5906455508407689
1113	7	-0.6446588286126129
1113	8	-0.5162124129817762
1113	9	-0.5495116408601532
1113	10	-0.5200673080807903
1113	11	-0.6139116026370672
1113	12	-0.6964435866274741
1113	13	-0.642544095369395
1113	14	-0.3334296544481248
1113	15	-0.5973836495638025
1113	16	-0.6987100239417856
1113	17	-0.5522976937111181
1113	18	-0.7847638987841137
1113	19	-1.0658889417627293
1113	20	-0.8954048779817825
1113	21	-0.5226770679645014
1113	22	-1.0329532983493905
1113	23	-4.674335217655443
1113	24	-2.166154600953682
1113	25	-1.2558692352678236
1114	1	-0.6279553961434559
1114	2	-0.8668198522858894
1114	3	-0.6881865088860017
1114	4	-0.7459051314821714
1114	5	-0.6210888593651873
1114	6	-1.2677299496072791
1114	7	-1.088380958350335
1114	8	-1.009139700180235
1114	9	-1.1672198896936123
1114	10	-0.9489700706240995
1114	11	-0.9868248231785017
1114	12	-1.9692467222046517
1114	13	-1.255470459682293
1114	14	-3.0112568438324736
1114	15	-1.817544907650806
1114	16	-1.8818769439611174
1114	17	-1.5921812661129593
1114	18	-1.5931521349910867
1114	19	-1.627235849155575
1114	20	-1.360983230378288
1114	21	-1.1050062489006223
1114	22	-1.1893184753494381
1114	23	-5.645293272800501
1114	24	-2.823045954985517
1114	25	-2.6220306955666963
1115	1	-1.7654275434754538
1115	2	-1.5946431446241967
1115	3	-1.3484104510062558
1115	4	-1.3163288154512927
1115	5	-1.277235232741205
1115	6	-1.4841353713324956
1115	7	-1.9818956656693842
1115	8	-2.2166541024156494
1115	9	-3.585311379164242
1115	10	-4.1840677149000065
1115	11	-2.997894351471057
1115	12	-3.1652365043619675
1115	13	-2.3947907700031763
1115	14	-3.1833477122559515
1115	15	-2.831497528474994
1115	16	-3.0211935282971787
1115	17	-2.9818618977955573
1115	18	-5.555947133318494
1115	19	-3.3781187398878636
1115	20	-3.8540710441648933
1115	21	-3.331952630712273
1115	22	-2.8502964077572415
1115	23	-3.5478712891472917
1115	24	-3.3473678052559244
1115	25	-3.4312660109575255
1116	1	-1.6129969576664287
1116	2	-1.3759595196831995
1116	3	-1.8602317178460197
1116	4	-1.5814744702786356
1116	5	-1.7900018978673131
1116	6	-4.0389362717262465
1116	7	-2.421404116142046
1116	8	-2.7947376517429667
1116	9	-3.9589887269576174
1116	10	-3.283618275676208
1116	11	-2.77238299312965
1116	12	-2.6965450377217692
1116	13	-3.999668382671725
1116	14	-7.234846234455539
1116	15	-4.961220144465848
1116	16	-3.79189322758506
1116	17	-4.7266780800321015
1116	18	-3.5064164609098327
1116	19	-3.2094558367317134
1116	20	-3.9266552515130577
1116	21	-4.7193412842660045
1116	22	-4.373057110161348
1116	23	-3.359928564615199
1116	24	-4.258477636776828
1116	25	-4.171737740306014
1201	1	0.5548895552285273
1201	2	0.6799632704612577
1201	3	0.9511400136524557
1201	4	0.895980942532546
1201	5	0.5475904259101796
1201	6	0.8907469018456524
1201	7	0.9477520685119032
1201	8	0.9774203745904941
1201	9	1.3592680402553725
1201	10	1.2300392486716873
1201	11	1.4589755972181635
1201	12	1.1599411273003923
1201	13	1.4382021114483265
1201	14	1.504610629957206
1201	15	1.4214084373769822
1201	16	1.4401804523847588
1201	17	1.633017595218412
1201	18	1.52565913747614
1201	19	1.74369116510357
1201	20	1.6880564425123552
1201	21	1.8027436823855325
1201	22	1.5733843877379883
1201	23	2.0011857793823964
1201	24	1.8539381927143452
1201	25	1.9575740598717601
1202	1	0.9227388098520031
1202	2	0.825121170122749
1202	3	0.9743877463129174
1202	4	1.1435240039780226
1202	5	0.8785969135206813
1202	6	1.1468561607412582
1202	7	1.2499438916234098
1202	8	1.199230601980041
1202	9	1.4369669330094297
1202	10	1.2387649586061258
1202	11	1.2886258824477181
1202	12	1.4995672909797406
1202	13	1.4386888823000632
1202	14	1.841354873195506
1202	15	1.4780624548754986
1202	16	1.2979674932919778
1202	17	1.5667365799279906
1202	18	1.6407766734768643
1202	19	1.6407634454195659
1202	20	1.5536661357005173
1202	21	1.6343318173036963
1202	22	1.653177795601792
1202	23	1.6903095672260235
1202	24	1.8072053438844762
1202	25	2.084066921284112
1203	1	1.1301398627103367
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2211	18	0.003794089584005178
2211	19	0.13008095218184934
2211	20	0.14789110787749138
2211	21	0.1556345981319751
2211	22	0.22065087544465456
2211	23	0.4221210487375302
2211	24	0.05897967889578837
2211	25	0.42219610855701717
2212	1	0.05723040458219941
2212	2	0.07518825405987428
2212	3	-0.2919712548958664
2212	4	0.11497088241522846
2212	5	-0.13464520458067142
2212	6	-0.3082567706182786
2212	7	-0.12690517421229686
2212	8	-0.01646578023253653
2212	9	0.14081931588837193
2212	10	-0.29543169241526357
2212	11	-0.39088043508699144
2212	12	0.01615792918433101
2212	13	0.07508528239441858
2212	14	0.19145333210989435
2212	15	-0.30948455391597207
2212	16	-0.21896251285274815
2212	17	-0.004419517752630497
2212	18	0.14383087576970865
2212	19	-0.1411956005919066
2212	20	0.027132519024426927
2212	21	0.1603414987876377
2212	22	0.24862006160439165
2212	23	0.20605305921077388
2212	24	0.2840167175357067
2212	25	0.4341752053479624
2213	1	-0.24440810147615366
2213	2	-0.20486448284378156
2213	3	-0.35315720473448664
2213	4	-0.14141912711126992
2213	5	-0.2045488081539304
2213	6	0.07409916807010575
2213	7	-0.25886878177580286
2213	8	-0.0816849458563027
2213	9	-0.5263228120534649
2213	10	-0.2617812553450065
2213	11	-0.48049668040255256
2213	12	-0.4438312055970641
2213	13	-0.47989030541131683
2213	14	-0.4181361733671949
2213	15	-0.3548410116819696
2213	16	-0.31629021320073036
2213	17	-0.26991389815447064
2213	18	-0.38008124205831706
2213	19	-0.30959396545423346
2213	20	-0.016118046908360384
2213	21	-0.20959587505966226
2213	22	-0.5166542938022616
2213	23	-0.33942301960265553
2213	24	-0.037151112089375715
2213	25	0.16302149705230562
2214	1	-2.6612070549209896
2214	2	-1.349308749423649
2214	3	-1.5168553116328738
2214	4	-3.3101456878951545
2214	5	-1.809815733969284
2214	6	-1.5836720465048302
2214	7	-1.3578553094235168
2214	8	-1.213856779044228
2214	9	-1.0281930580668543
2214	10	-1.0915944263946997
2214	11	-1.1335124632156806
2214	12	-1.0861108602907734
2214	13	-1.4259310844225865
2214	14	-1.4683831383305375
2214	15	-1.7235785111372621
2214	16	-1.5485348819324045
2214	17	-3.673248947880957
2214	18	-3.4093003898913494
2214	19	-2.249893275962208
2214	20	-2.1854455676273616
2214	21	-2.025914238420981
2214	22	-2.9031955926227546
2214	23	-1.957717858595192
2214	24	-3.0952305659739814
2214	25	-2.999857098813589
2215	1	-2.0960273163043617
2215	2	-3.7411742016266736
2215	3	-2.1933578432488687
2215	4	-3.862641869054503
2215	5	-2.4719614273294503
2215	6	-2.2207272058058365
2215	7	-2.3823706532081808
2215	8	-1.7829696175804202
2215	9	-4.325986085247653
2215	10	-2.564717242312671
2215	11	-2.1686891868117186
2215	12	-2.015752295351568
2215	13	-2.1449685474440834
2215	14	-1.9281150351345033
2215	15	-2.040586898679777
2215	16	-2.221484584491165
2215	17	-2.56230064046456
2215	18	-2.1902358918143996
2215	19	-2.342812872817241
2215	20	-2.2126935658650737
2215	21	-2.728959631725413
2215	22	-2.6683719935782158
2215	23	-2.3941079332809245
2215	24	-2.4672438320645647
2215	25	-3.3670109527553116
2216	1	-3.4042732057532596
2216	2	-3.5777216141139334
2216	3	-4.823879201589779
2216	4	-5.356144857294332
2216	5	-5.0621195148559615
2216	6	-3.9575596715856705
2216	7	-5.721884272421047
2216	8	-3.432906272404292
2216	9	-4.277751487764938
2216	10	-3.3045627351656854
2216	11	-3.5729553467169772
2216	12	-3.8759398521556676
2216	13	-3.4241403781196738
2216	14	-4.526214679850693
2216	15	-3.8512591837881516
2216	16	-4.267245965966833
2216	17	-3.8352839228463402
2216	18	-3.428866809768625
2216	19	-4.210129516073735
2216	20	-4.744546549973698
2216	21	-4.507314860456582
2216	22	-3.979881536666703
2216	23	-4.09007449804448
2216	24	-4.542083403520678
2216	25	-4.417776603145022
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	124902.0
3	2.0
4	1152.0
5	6990.0
6	8320.0
7	7228.0
8	6435.0
9	6247.0
10	5338.0
11	5061.0
12	4751.0
13	4640.0
14	4696.0
15	4706.0
16	4825.0
17	5213.0
18	5905.0
19	7303.0
20	9387.0
21	11869.0
22	14886.0
23	17637.0
24	20492.0
25	23490.0
26	26059.0
27	28151.0
28	32082.0
29	38070.0
30	45933.0
31	56718.0
32	71100.0
33	90298.0
34	117266.0
35	153479.0
36	207131.0
37	316386.0
38	878403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99794686720692	18.10309137096409	12.382103448290446	24.51685831353854
2	14.978023335268173	19.887964746285128	41.722614740446886	23.411397177999813
3	19.64977477572417	25.19063761696445	28.61275672321652	26.546830884094856
4	12.385705319004986	16.161165669800237	37.573932445209586	33.87919656598519
5	13.23020954966424	38.80648166493131	34.428449180728684	13.53485960467577
6	32.175137060466895	35.98891283430964	18.314450477903073	13.521499627320388
7	29.004423229590337	31.429184878848393	21.213194941843348	18.35319694971793
8	25.903073328942522	36.541143183349575	20.346221532331324	17.209561955376586
9	28.08510045925799	14.568901986574497	19.500473094366193	37.845524459801325
10	16.556988170415874	28.489465759764226	33.04997905428294	21.903567015536954
11	35.06674738083235	22.140176882141148	24.187841831170996	18.605233905855503
12	24.50775733100899	24.341995904270984	30.93752030355984	20.212726461160184
13	29.81461647457035	21.73931567506778	25.398095586811603	23.047972263550268
14	21.734748632764052	22.23865586489802	27.008541744358517	29.018053757979413
15	23.723819366813608	30.917128936322797	22.90582354305963	22.45322815380397
16	22.393530519739382	27.543803772597936	27.817077723617018	22.245587984045663
17	21.988660263120035	28.341959009369898	27.389147722454478	22.280233005055585
18	21.7887286803717	26.747603025093085	29.928085478920334	21.53558281561488
19	24.177873329969508	26.211821546156717	27.168276297337567	22.44202882653621
20	24.531095817651867	26.519522082445462	27.712248778353732	21.237133321548942
21	24.019078488906736	26.05204190619532	26.581732772919242	23.347146831978698
22	24.08050193677355	27.698329957357654	26.586473004781496	21.6346951010873
23	22.995755847311756	27.29766445036957	27.3793411860002	22.32723851631847
24	23.77121035832438	26.31472370742425	27.920218425242428	21.993847509008944
25	23.226972420016274	26.75090098888355	27.811585221546448	22.210541369553727
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	308.0
1	308.0
2	735.0
3	1162.0
4	1162.0
5	1162.0
6	1943.5
7	2725.0
8	2725.0
9	2725.0
10	3223.0
11	3721.0
12	3721.0
13	3721.0
14	5461.5
15	7202.0
16	7202.0
17	7202.0
18	14023.0
19	20844.0
20	20844.0
21	20844.0
22	34459.0
23	48074.0
24	48074.0
25	48074.0
26	75143.0
27	102212.0
28	102212.0
29	102212.0
30	135752.5
31	169293.0
32	169293.0
33	169293.0
34	192195.0
35	215097.0
36	215097.0
37	215097.0
38	231099.0
39	247101.0
40	247101.0
41	247101.0
42	264412.0
43	281723.0
44	281723.0
45	281723.0
46	302378.0
47	323033.0
48	323033.0
49	323033.0
50	313330.0
51	303627.0
52	303627.0
53	303627.0
54	270056.5
55	236486.0
56	236486.0
57	236486.0
58	205804.5
59	175123.0
60	175123.0
61	175123.0
62	145959.5
63	116796.0
64	116796.0
65	116796.0
66	91073.0
67	65350.0
68	65350.0
69	65350.0
70	48631.0
71	31912.0
72	31912.0
73	31912.0
74	22621.0
75	13330.0
76	13330.0
77	13330.0
78	9194.5
79	5059.0
80	5059.0
81	5059.0
82	3389.0
83	1719.0
84	1719.0
85	1719.0
86	1111.5
87	504.0
88	504.0
89	504.0
90	308.5
91	113.0
92	113.0
93	113.0
94	70.5
95	28.0
96	28.0
97	28.0
98	18.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3317947643696595
2	0.011042965988929216
3	0.010495032561997613
4	0.011970237942198082
5	0.01352974077269572
6	0.07932390072963658
7	0.07945034690508233
8	0.10326437661403273
9	0.08379166559538657
10	0.0901139743676743
11	0.0862362916540045
12	0.09597264716332758
13	0.08227431149003751
14	0.08745860468331344
15	0.08433959902231818
16	0.08581480440251864
17	0.09702636529204219
18	0.10014537095303748
19	0.094202400707087
20	0.0920528157245092
21	0.08105199846072855
22	0.09997677605244314
23	0.09403380580649268
24	0.08973463584133702
25	0.08851232281202806
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2372551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0472400145259
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64345799401087	38.3293873371394
2	14.104793114233228	14.682311051434581
3	5.2551852353342845	8.205536617927088
4	2.3999519934964666	4.99643509715402
5	1.321725008066347	3.4396069364015167
6	0.8039621801584731	2.5106407531985737
7	0.5206924177736219	1.8970422269125244
8	0.36620352332575756	1.5247906138160592
9	0.26024987766561714	1.219075906195204
>10	1.1997417439262972	10.623343501267076
>50	0.06867418696110235	2.481048233513834
>100	0.04728536315884037	4.700348105957518
>500	0.004732191813785876	1.8013266609515972
>1k	0.0031004015331698416	2.8407871387272925
>5k	2.4476854209235595E-4	0.7483198194037453
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6738	0.2839981100511643	No Hit
GTCCTACAGTGGACATTTCTAAATT	5739	0.24189153362772814	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5145	0.21685519088946878	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4753	0.20033289063122353	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4041	0.17032299832543113	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3915	0.16501225895670948	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3734	0.15738333970481563	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2830	0.11928089217049496	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2709	0.1141808964275162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.214872514858479E-5
2	0.0	0.0	0.0	0.0	4.214872514858479E-5
3	0.0	0.0	0.0	0.0	4.214872514858479E-5
4	0.0	0.0	0.0	0.0	4.214872514858479E-5
5	0.0	0.0	0.0	0.0	4.214872514858479E-5
6	0.0	0.0	0.0	0.0	4.214872514858479E-5
7	0.0	0.0	0.0	0.0	4.214872514858479E-5
8	0.0	0.0	0.0	0.0	8.429745029716958E-5
9	0.0	0.0	0.0	0.0	8.429745029716958E-5
10	0.0	0.0	0.0	0.0	8.429745029716958E-5
11	0.0	0.0	0.0	0.0	8.429745029716958E-5
12	0.0	0.0	0.0	0.0	8.429745029716958E-5
13	0.0	0.0	0.0	0.0	8.429745029716958E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	885	0.0	11.913508	4
ATAGGAC	265	0.0	11.828456	3
TAGAAAT	740	0.0	11.424001	4
GATATAC	600	0.0	11.114769	1
TCCAACG	265	0.0	11.111345	18
CCGATCA	60	0.0058845403	11.081475	9
ATTTAGA	760	0.0	11.031199	1
GTCCCGC	465	0.0	11.030176	12
TGTAGAA	920	0.0	10.944048	2
GGCGAGG	1120	0.0	10.94013	19
CTAAGAC	270	0.0	10.905808	3
CGCCCTA	445	0.0	10.885816	16
CCCGCCC	450	0.0	10.764861	14
TCCCGCC	460	0.0	10.73733	13
CGGTTTC	195	1.8189894E-12	10.716151	13
TTTAGAA	860	0.0	10.713547	2
GTCTTAC	205	0.0	10.688767	1
AAATGTC	730	0.0	10.669894	7
GTCCTAT	260	0.0	10.626207	1
GTCCTAA	1390	0.0	10.623571	1
>>END_MODULE