FastQCFastQC Report
Thu 26 May 2016
SRR1512919_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512919_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2372551
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82150.34625177709562405No Hit
TATCAACGCAGAGTACTTTTTTTTT59360.2501948324819993No Hit
GTCCTACAGTGGACATTTCTAAATT55040.2319865832178107No Hit
GGTATCAACGCAGAGTACTTTTTTT47320.19944776740310324No Hit
CTGTAGGACGTGGAATATGGCAAGA46480.1959072744906221No Hit
GTCCTAAAGTGTGTATTTCTCATTT46250.1949378538122047No Hit
CTTTAGGACGTGAAATATGGCGAGG41280.17398993741335803No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29010.12227345165604449No Hit
GTCCTACAGTGTGCATTTCTCATTT25980.1095023879360233No Hit
GTACTGGTTCACTATCGGTCAGTCA24740.10427594601759876No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9050.012.926214
TCGGACT701.07990585E-412.22810654
CGTTTTA550.003089892712.08009917
GGTATCA22400.011.7213241
CCAACGA1406.91216E-1011.52491519
AGGACCT17900.011.1578635
GGACCTG16750.011.1289976
AATGTCC6900.010.8776338
GCGTGCG1408.38736E-910.8547569
TCCCGCC3350.010.76761913
GGCGAGG9950.010.49268119
TAGAAAT6650.010.4403714
GTCGAGG1106.116363E-610.35393419
ACGGTAC2850.010.34699253
TGTAGGA27450.010.3269292
GTCCTAC25850.010.30417251
GTATCAA46000.010.2988131
AAATGTC6600.010.226517
CCGTACA1951.6370905E-1110.22162217
TGTCCAC8000.010.20874810
Produced by FastQC (version 0.11.5)