##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512919_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2372551
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19586976212524	31.0	30.0	34.0	26.0	34.0
2	30.212519773020684	31.0	30.0	34.0	26.0	34.0
3	30.39308744048073	31.0	30.0	34.0	26.0	34.0
4	33.97380583178191	37.0	35.0	37.0	30.0	37.0
5	33.536477824923466	37.0	35.0	37.0	28.0	37.0
6	33.39639653689215	37.0	35.0	37.0	27.0	37.0
7	33.236560141383684	37.0	35.0	37.0	26.0	37.0
8	33.18104015466896	37.0	35.0	37.0	26.0	37.0
9	34.50486796701104	39.0	35.0	39.0	27.0	39.0
10	34.42741926306326	39.0	35.0	39.0	26.0	39.0
11	34.40008286439364	39.0	35.0	39.0	26.0	39.0
12	34.26499240690716	39.0	35.0	39.0	25.0	39.0
13	34.25101125328813	39.0	35.0	39.0	25.0	39.0
14	35.31995898086068	40.0	36.0	41.0	25.0	41.0
15	35.32097855852203	40.0	36.0	41.0	25.0	41.0
16	35.314599348970795	40.0	36.0	41.0	25.0	41.0
17	35.30548974500443	40.0	36.0	41.0	25.0	41.0
18	35.225475026669606	40.0	36.0	41.0	25.0	41.0
19	35.23648848855093	40.0	35.0	41.0	25.0	41.0
20	35.2105518490435	40.0	35.0	41.0	25.0	41.0
21	35.1763431007384	40.0	35.0	41.0	25.0	41.0
22	35.134969912132554	40.0	35.0	41.0	25.0	41.0
23	34.98009147116332	40.0	35.0	41.0	23.0	41.0
24	34.9359630203945	40.0	35.0	41.0	23.0	41.0
25	34.87105356217843	40.0	35.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5933999142260156
1101	2	0.3452651839901826
1101	3	0.3400198794268938
1101	4	0.4850819126780195
1101	5	0.556135137522979
1101	6	0.532848225447303
1101	7	0.6194624567292308
1101	8	0.3451928071696173
1101	9	0.6222009249563385
1101	10	0.3268527137993047
1101	11	0.6477239503151395
1101	12	0.673534837617936
1101	13	0.885996080866704
1101	14	0.8225878991916602
1101	15	0.49832027777206633
1101	16	0.6726377764458107
1101	17	0.7711586980966771
1101	18	0.6842206959071575
1101	19	0.4729996110825141
1101	20	0.624432979074264
1101	21	0.6205369580156201
1101	22	0.6114773322964879
1101	23	0.5538861836609925
1101	24	0.7461609103236739
1101	25	0.6349378716872209
1102	1	0.7528042615919333
1102	2	0.8259694789043053
1102	3	0.5190666055801678
1102	4	0.5786751367479681
1102	5	0.8475488020416577
1102	6	0.7652321584055599
1102	7	0.8297502505937544
1102	8	0.9118426084932594
1102	9	0.9926355989348181
1102	10	0.9131952108200849
1102	11	1.111076379682686
1102	12	1.0499434187708516
1102	13	1.1423155490373347
1102	14	1.1740727119716468
1102	15	1.1588418189541159
1102	16	1.236604034393828
1102	17	1.2245559985303487
1102	18	0.6417052457115062
1102	19	0.9402207092419061
1102	20	1.127791048160212
1102	21	1.0527947441425027
1102	22	0.9307576631901426
1102	23	1.0595760303799935
1102	24	1.100532118308962
1102	25	1.1810704438446535
1103	1	0.7745966887235056
1103	2	0.9948607891739876
1103	3	0.7108399643379997
1103	4	0.6320665832203218
1103	5	0.6514169578575135
1103	6	0.7181060347335375
1103	7	0.8722454835003148
1103	8	0.924645659433061
1103	9	0.9708431718032529
1103	10	1.0363224241134645
1103	11	1.0674915254195412
1103	12	1.1627192291767372
1103	13	1.1355054155587183
1103	14	1.2271917531048473
1103	15	1.224763911027118
1103	16	1.1924743694523912
1103	17	1.191594952493844
1103	18	1.3093707319550347
1103	19	1.1785753809934718
1103	20	1.0351732328510366
1103	21	1.2312202412822444
1103	22	1.1350616675486265
1103	23	1.2794071390697255
1103	24	1.3026568799542915
1103	25	1.2674229363535048
1104	1	0.8560092123572716
1104	2	0.9560601178569179
1104	3	0.9088580357991454
1104	4	0.8196664340061517
1104	5	0.8748629015337102
1104	6	0.7806779958774186
1104	7	0.7295394115016904
1104	8	0.8262135230549461
1104	9	0.9370060132799196
1104	10	0.8879670585130839
1104	11	1.0550860806573112
1104	12	1.0089323328857134
1104	13	0.9173177604053961
1104	14	1.302721524279967
1104	15	1.1963941491639574
1104	16	1.1207843106923505
1104	17	1.0636439496622714
1104	18	0.6343030670262166
1104	19	1.0686753025577502
1104	20	1.0703189491707263
1104	21	1.0656513373272034
1104	22	0.9978623787656673
1104	23	0.9884262348746944
1104	24	1.2909390496386166
1104	25	1.0748087443140477
1105	1	0.5103261919642001
1105	2	0.4803968062129691
1105	3	0.4979647753531644
1105	4	0.3867116315127319
1105	5	0.32134888265969863
1105	6	0.4622968747119529
1105	7	0.5514377377793735
1105	8	0.5017100882327483
1105	9	0.7194648821022014
1105	10	0.4593257336350476
1105	11	0.6791530295500507
1105	12	0.790531204440704
1105	13	0.8452523719082308
1105	14	0.8427389538640995
1105	15	0.867288896521103
1105	16	0.7211858341160777
1105	17	0.47619347987871663
1105	18	0.5593874305765709
1105	19	0.7327934092318529
1105	20	0.8206335543125647
1105	21	0.6576299969944799
1105	22	0.7408705952415602
1105	23	0.944014236477571
1105	24	0.8287321404963492
1105	25	0.5933210753429563
1106	1	0.03428010147730021
1106	2	0.3510138287324658
1106	3	-1.549505505337315
1106	4	-0.27770508140019956
1106	5	-0.05173973491777417
1106	6	0.056944916304381366
1106	7	0.1879907359976798
1106	8	0.28800415605411445
1106	9	0.49827289682311715
1106	10	0.3028393300366048
1106	11	0.35703310081797923
1106	12	0.4654299981434207
1106	13	0.4478362723667999
1106	14	0.46321679946973404
1106	15	0.6752938327012572
1106	16	0.5857358823095566
1106	17	0.5963570456116898
1106	18	0.7059061091991126
1106	19	0.6647145763901889
1106	20	0.6086351315535623
1106	21	0.43047720907497933
1106	22	0.5048337053271439
1106	23	0.5737223008523742
1106	24	0.5532521042782221
1106	25	0.5354047859484439
1107	1	0.5361499823743294
1107	2	0.6752857984023386
1107	3	0.5974804383659631
1107	4	0.35128808716806503
1107	5	0.549763180064879
1107	6	0.5041790806164101
1107	7	0.5330467108179491
1107	8	0.5576668764661434
1107	9	0.501119922825481
1107	10	0.48370416783480863
1107	11	0.5307210009574703
1107	12	0.8089930726453716
1107	13	0.6937148803284572
1107	14	0.7651982723407187
1107	15	0.6555728126361586
1107	16	0.8349794174860605
1107	17	0.7304898829806987
1107	18	0.7218000968238485
1107	19	0.6701968897776496
1107	20	0.7520622927538909
1107	21	0.6570217922145716
1107	22	0.7691757776473267
1107	23	0.9195687188215445
1107	24	0.9175898845994084
1107	25	0.8663379380640421
1108	1	0.6611740665788339
1108	2	0.6024449515914974
1108	3	0.6481069680959131
1108	4	0.5738349875394562
1108	5	0.727515502531098
1108	6	0.7395506922326547
1108	7	0.7774654730089807
1108	8	0.6036995356686958
1108	9	0.7574895566434492
1108	10	0.7302937423528704
1108	11	0.8302209345769782
1108	12	0.8822366277803297
1108	13	0.9470500745850146
1108	14	1.0264269582452457
1108	15	0.934154721058313
1108	16	0.9464652852493103
1108	17	0.8820079537933623
1108	18	0.9683379285986575
1108	19	0.9543632699302265
1108	20	0.945916480341765
1108	21	1.0106425927973604
1108	22	0.9683199799069868
1108	23	1.0196436096341444
1108	24	0.950197684817951
1108	25	0.9973672442006603
1109	1	0.41715572372939747
1109	2	0.37850483559994785
1109	3	0.5216626934926687
1109	4	0.4134609372764473
1109	5	0.07704119600769843
1109	6	0.36921845287645283
1109	7	0.4640410248679956
1109	8	0.5161753707628804
1109	9	0.694453994893685
1109	10	0.3343534465294624
1109	11	0.4893451534323461
1109	12	0.8109377921916021
1109	13	0.6405356655574508
1109	14	0.810889485781999
1109	15	0.7668886148676037
1109	16	0.699281901712915
1109	17	0.8806592148642665
1109	18	0.9149908081562756
1109	19	0.855518689612822
1109	20	0.8162082575300929
1109	21	0.8510193405382225
1109	22	0.8533624651832525
1109	23	0.7454449853478522
1109	24	0.739443739756581
1109	25	0.7101128270806711
1110	1	0.5037885874013988
1110	2	0.394150450162126
1110	3	0.3965831762005294
1110	4	0.3594749207961172
1110	5	0.4568314912282929
1110	6	0.6150689089219341
1110	7	0.5503624729641388
1110	8	0.5448302681157813
1110	9	0.5115450167277587
1110	10	0.6192008151707356
1110	11	0.5002861977994613
1110	12	0.7260292379349877
1110	13	0.6900943677364708
1110	14	0.7932843538728136
1110	15	0.8080620754011676
1110	16	0.72110753779981
1110	17	0.7474361802635272
1110	18	0.644090535907722
1110	19	0.8387403866356493
1110	20	0.9920902186799694
1110	21	0.9113286708644068
1110	22	0.8747649464931726
1110	23	0.94874516732704
1110	24	0.9051194326733878
1110	25	0.8265346483953522
1111	1	0.369300922094947
1111	2	0.14777469680595345
1111	3	0.14872476022036096
1111	4	0.37616061929574585
1111	5	0.5089951444020926
1111	6	0.47812820635924425
1111	7	0.37355289267417646
1111	8	0.3355838721136166
1111	9	0.41482573929790334
1111	10	0.38010133755586395
1111	11	0.4238639969532514
1111	12	0.503383040698381
1111	13	0.5669024685681663
1111	14	0.41201902707718574
1111	15	0.4033513575577885
1111	16	0.3267649589431514
1111	17	0.4603788016222268
1111	18	0.2834934456407936
1111	19	0.18041104681697107
1111	20	0.4248310801105646
1111	21	0.5667490002981737
1111	22	0.23495133369085153
1111	23	0.36780881205361027
1111	24	0.48764610331755165
1111	25	0.36196654896539115
1112	1	0.12080133381932612
1112	2	-0.32505145987268236
1112	3	-3.865593084206864E-4
1112	4	0.07780777064532884
1112	5	0.036926439838673275
1112	6	0.08879795634516086
1112	7	0.16991881016918597
1112	8	0.04192179317408318
1112	9	0.13954345100631826
1112	10	-0.1900547060867197
1112	11	-0.09730037866259522
1112	12	0.2765214328459038
1112	13	0.12525410019109273
1112	14	-0.0756783274110262
1112	15	-0.39128115330782265
1112	16	-0.36256791858800597
1112	17	-0.15028381359148568
1112	18	0.019803927327785686
1112	19	0.01550601715243971
1112	20	-0.044419595240213994
1112	21	-0.06898575329389445
1112	22	-0.029713933325595576
1112	23	0.1928049239457721
1112	24	0.12829307897336406
1112	25	0.05314335349163457
1113	1	-0.6869913568199095
1113	2	-0.6617344228136872
1113	3	-0.616433284078429
1113	4	-2.0514946022371454
1113	5	-0.8797118174563607
1113	6	-0.45598468314073415
1113	7	-0.592277456592349
1113	8	-0.4944607303686368
1113	9	-0.3816198839409992
1113	10	-0.5956997717494232
1113	11	-0.781099955074744
1113	12	-0.3603520375770586
1113	13	-0.5552586354420015
1113	14	-0.6247203139919222
1113	15	-0.6122933009904372
1113	16	-0.8144317380592128
1113	17	-0.9123227994839667
1113	18	-0.6227652103777856
1113	19	-0.4381663264477922
1113	20	-0.22190240962902408
1113	21	-0.42146762070365185
1113	22	-0.4667042115562765
1113	23	-0.244719985720657
1113	24	-0.24248051233353607
1113	25	-0.4797538754006112
1114	1	-0.9605696868790545
1114	2	-2.121478757060501
1114	3	-1.4800793668700827
1114	4	-1.042728879382615
1114	5	-0.8989923370468063
1114	6	-0.521269872414571
1114	7	-0.6926080494845976
1114	8	-0.6914272878173833
1114	9	-1.0022397044662057
1114	10	-1.114329568843921
1114	11	-0.9360007125462957
1114	12	-1.0610686258866266
1114	13	-1.066678168638255
1114	14	-1.106336668699754
1114	15	-1.323371190180687
1114	16	-1.5417907687589718
1114	17	-1.5574624256036245
1114	18	-1.3218829876226081
1114	19	-1.7158445903178219
1114	20	-1.0433584408919216
1114	21	-1.399190000854908
1114	22	-0.9610020866857454
1114	23	-0.8764589607651274
1114	24	-0.9633390780635622
1114	25	-1.5795172169752547
1115	1	-1.158877031592489
1115	2	-1.2813676958858657
1115	3	-0.8354641713054356
1115	4	-0.6260069128912917
1115	5	-1.0146066651276726
1115	6	-0.7578782544943437
1115	7	-0.7267811427919781
1115	8	-1.0226865641223313
1115	9	-2.0830553463889316
1115	10	-1.628414722948044
1115	11	-2.0334931918322425
1115	12	-2.549653736061387
1115	13	-2.6301247969983166
1115	14	-2.2039321342060347
1115	15	-2.403154510750035
1115	16	-2.229952145036414
1115	17	-1.9713840909459535
1115	18	-2.384806226895435
1115	19	-2.874911651412276
1115	20	-2.952361545456995
1115	21	-2.3000980308067582
1115	22	-2.245017291386503
1115	23	-2.2452687120548944
1115	24	-2.767386481234716
1115	25	-2.689225710081921
1116	1	-1.7545134101063837
1116	2	-1.8590827989985286
1116	3	-1.9391100689602752
1116	4	-1.5654376282588274
1116	5	-1.9770950067560307
1116	6	-2.730950617088453
1116	7	-2.299745691492717
1116	8	-2.6066716171617657
1116	9	-3.6585550747706534
1116	10	-2.292573763214982
1116	11	-3.4344163151469402
1116	12	-5.455976342808885
1116	13	-4.314621719794328
1116	14	-3.6755157861952164
1116	15	-4.7732365720486385
1116	16	-4.348844427364863
1116	17	-3.988577565743036
1116	18	-3.9281588525766615
1116	19	-3.9914699864566394
1116	20	-5.935996790523351
1116	21	-4.792028126793703
1116	22	-3.9597423356523294
1116	23	-4.862083512334014
1116	24	-4.606221682054194
1116	25	-3.9848924704448976
1201	1	0.47804243179054495
1201	2	0.7465368813933395
1201	3	0.712114520019064
1201	4	0.6116342611892449
1201	5	0.07552363129639161
1201	6	0.6463383731212318
1201	7	0.8702395686764888
1201	8	0.7467706608934748
1201	9	0.8148457654956189
1201	10	0.8396911188983509
1201	11	0.5438969494218355
1201	12	1.0184590033693084
1201	13	0.9778811098695002
1201	14	0.883356568788507
1201	15	0.8387882202828152
1201	16	0.9181727232755676
1201	17	0.7846357493440266
1201	18	1.0225811764553683
1201	19	1.2025034690584846
1201	20	1.2177388453356812
1201	21	0.8850223403406332
1201	22	0.9472032900390985
1201	23	0.9813553445457472
1201	24	0.6763053836004005
1201	25	0.9019895597509731
1202	1	0.9149784439692041
1202	2	1.044425560916217
1202	3	0.9506357769620521
1202	4	0.9266804974948712
1202	5	0.8719412255926642
1202	6	0.9928089535712346
1202	7	1.0465787348470954
1202	8	0.8808059312352015
1202	9	1.2404741661971386
1202	10	1.2399421836999664
1202	11	1.1778491162985176
1202	12	1.2375770632296579
1202	13	1.2141350272745086
1202	14	1.3335452752122876
1202	15	1.247394799861155
1202	16	1.3535846332948296
1202	17	1.3066173083773478
1202	18	1.2414602369060574
1202	19	1.3783056945077234
1202	20	1.197646043388481
1202	21	1.339043578508388
1202	22	1.2773821286958764
1202	23	1.394162958177425
1202	24	1.4582880599136345
1202	25	1.4739695490521711
1203	1	0.8939004665742694
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2211	13	0.19802509132274082
2211	14	0.07435711787032062
2211	15	0.11708576161647244
2211	16	0.25761296487156926
2211	17	0.09268803413446136
2211	18	-0.07422347243731764
2211	19	-0.016081425090739287
2211	20	0.060926875352159016
2211	21	0.18336502840115543
2211	22	0.11115728918497325
2211	23	0.10295591803119919
2211	24	0.2142591387099131
2211	25	0.060950879987686335
2212	1	-0.26725493477087525
2212	2	-0.17896529611992662
2212	3	-0.19075962278652625
2212	4	-0.11376614943270624
2212	5	-0.3602156297889252
2212	6	-0.1936570995548763
2212	7	-0.42621211486748933
2212	8	-0.22762405427670984
2212	9	-0.13350704768042476
2212	10	-0.4290630868740948
2212	11	-0.1473321813621027
2212	12	0.05474755264364006
2212	13	-0.055677113684510005
2212	14	0.007023899944634593
2212	15	-0.1418659042207011
2212	16	-0.13499705106911364
2212	17	-0.3509597850217645
2212	18	-0.4011902711014699
2212	19	-0.10026372588632881
2212	20	-0.14789805846877613
2212	21	-0.09714254883180473
2212	22	0.007254491270963115
2212	23	-0.4049756163855278
2212	24	-0.37609711025321957
2212	25	-0.27026645424005835
2213	1	-0.552524142585888
2213	2	-0.19445276700278313
2213	3	-0.2320036212215193
2213	4	-0.5167280011147
2213	5	-0.27634141067252926
2213	6	-0.29217021425375833
2213	7	-0.7538576185411969
2213	8	-0.6158660394506086
2213	9	-0.5351077241223337
2213	10	-0.48646110885155025
2213	11	-0.362808337907623
2213	12	-0.5090469565343554
2213	13	-0.34490094150620365
2213	14	-0.5027124759953878
2213	15	-0.7187053693202898
2213	16	-1.1566545064832852
2213	17	-0.8756999655156719
2213	18	-0.5183647010445611
2213	19	-0.5649662322279738
2213	20	-0.4449473483861439
2213	21	-0.4704627799897665
2213	22	-0.5335533437780882
2213	23	-0.6160860365447363
2213	24	-0.46499973181109056
2213	25	-0.5588336618356919
2214	1	-1.2684813074601244
2214	2	-0.6149132850689334
2214	3	-0.8100036852527666
2214	4	-0.9618345464421552
2214	5	-1.116843575729078
2214	6	-1.729631028464425
2214	7	-1.1235664809104762
2214	8	-1.004753149693741
2214	9	-0.7530109497853488
2214	10	-1.0619492804126054
2214	11	-1.1351877034824653
2214	12	-0.8213342416183664
2214	13	-1.2940965934287902
2214	14	-0.8379396223983875
2214	15	-1.0201111219942618
2214	16	-1.0421146110142914
2214	17	-1.1380846759210144
2214	18	-1.0949222833669623
2214	19	-1.1588006901100911
2214	20	-1.4192641483178434
2214	21	-1.467294344904552
2214	22	-1.9641103444833234
2214	23	-1.9504701048589652
2214	24	-1.6790424068587413
2214	25	-1.9575698006269775
2215	1	-1.61248269592717
2215	2	-1.0640890496436626
2215	3	-1.0785839443220269
2215	4	-1.1479518785828304
2215	5	-1.5792527218107182
2215	6	-1.4564830688323234
2215	7	-1.952688464638264
2215	8	-1.6624090212516371
2215	9	-1.7564883477001985
2215	10	-1.7394471025886915
2215	11	-1.5325869979008928
2215	12	-1.9206225410021247
2215	13	-2.0106067794143954
2215	14	-2.1622781813965517
2215	15	-1.8745325773702888
2215	16	-2.0173033279394588
2215	17	-2.1523157956062064
2215	18	-1.8436019602368603
2215	19	-2.2019898031043823
2215	20	-2.1957839245165403
2215	21	-1.732018492771843
2215	22	-2.084791939395835
2215	23	-2.3324367215719732
2215	24	-2.2762759307725204
2215	25	-2.5658981552824045
2216	1	-1.9485729214910776
2216	2	-1.282526627103799
2216	3	-1.31020971203802
2216	4	-1.605615267825165
2216	5	-2.463453608643185
2216	6	-2.731799106178439
2216	7	-2.668224557617968
2216	8	-2.820031098422586
2216	9	-4.679536352327151
2216	10	-2.687409040400251
2216	11	-2.8831736293145944
2216	12	-3.336723232290783
2216	13	-3.3362147553983093
2216	14	-3.3379205841066266
2216	15	-3.3435319990012893
2216	16	-2.629688686941364
2216	17	-2.9237856791061674
2216	18	-3.4881942100922636
2216	19	-3.646262572941062
2216	20	-2.9010346887898564
2216	21	-3.567757565728961
2216	22	-2.856555138602644
2216	23	-4.225084549934085
2216	24	-3.438822406852836
2216	25	-3.503894244406311
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69874.0
3	1.0
4	1166.0
5	10154.0
6	12244.0
7	9242.0
8	7587.0
9	6473.0
10	5528.0
11	5048.0
12	4545.0
13	4171.0
14	4085.0
15	4003.0
16	4143.0
17	4457.0
18	4695.0
19	5875.0
20	7440.0
21	10057.0
22	12521.0
23	14997.0
24	17826.0
25	20028.0
26	22403.0
27	24805.0
28	27988.0
29	32999.0
30	41354.0
31	52752.0
32	66731.0
33	86530.0
34	112015.0
35	147164.0
36	201413.0
37	312115.0
38	998122.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.5928356378022	19.0536413870432	13.476977176920524	26.876545798234076
2	13.395943258069018	20.304118601278766	40.67342776266082	25.626510377991394
3	17.60876796041973	25.153040081514575	29.395668210030728	27.842523748034964
4	11.589435517252134	16.84773491170552	36.84093168021271	34.72189789082963
5	12.87324388984328	38.76175921924897	34.7148382745065	13.650158616401253
6	32.183437827114176	36.429121501538944	17.871051328616	13.516389342730886
7	28.421771950064645	31.601765749272882	21.391845747923053	18.584616552739423
8	25.84334199251038	36.748085422219226	20.03951452380149	17.369058061468913
9	27.293760539629	14.444013490725126	19.56045531197302	38.70177065767285
10	15.907544052445456	28.86566376174528	33.18294257600433	22.043849609804933
11	34.79705748955908	22.009310418665347	24.239629471639642	18.954002620135928
12	23.97587736028388	24.534349851614913	31.04074404552415	20.449028742577056
13	29.43718181197411	21.60395006276297	25.67502859951324	23.283839525749677
14	21.330580713905167	22.070087265566517	27.209204696425076	29.390127324103233
15	23.57455913863363	30.8468273115055	22.926814462841097	22.65179908701977
16	22.30578279337917	27.59606479871485	27.656975354619572	22.44117705328641
17	21.77531224195859	28.37927550508148	27.43699627261228	22.40841598034765
18	21.606777572033202	26.782520581365894	30.005104507520404	21.605597339080507
19	23.88407026157152	26.185940098781625	27.440984343137593	22.48900529650926
20	24.30317675279039	26.43289805721097	27.858641265794937	21.405283924203705
21	23.629825200291332	25.929044940789296	26.913599916376686	23.52752994254269
22	23.793653710628625	27.469087929540375	26.962878540093804	21.77437981973719
23	22.645372006755597	27.098342669978432	27.683746313567127	22.572539009698843
24	23.69335790885001	26.1656335311654	28.04871212462872	22.09229643535587
25	22.931192177193317	26.692798887273188	28.01395123390445	22.36205770162905
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	129.0
1	129.0
2	365.0
3	601.0
4	601.0
5	601.0
6	1499.5
7	2398.0
8	2398.0
9	2398.0
10	2951.5
11	3505.0
12	3505.0
13	3505.0
14	5259.5
15	7014.0
16	7014.0
17	7014.0
18	14130.5
19	21247.0
20	21247.0
21	21247.0
22	36061.0
23	50875.0
24	50875.0
25	50875.0
26	78427.0
27	105979.0
28	105979.0
29	105979.0
30	138578.5
31	171178.0
32	171178.0
33	171178.0
34	193522.5
35	215867.0
36	215867.0
37	215867.0
38	232594.5
39	249322.0
40	249322.0
41	249322.0
42	265439.5
43	281557.0
44	281557.0
45	281557.0
46	302586.0
47	323615.0
48	323615.0
49	323615.0
50	313037.0
51	302459.0
52	302459.0
53	302459.0
54	268892.0
55	235325.0
56	235325.0
57	235325.0
58	204280.0
59	173235.0
60	173235.0
61	173235.0
62	144289.0
63	115343.0
64	115343.0
65	115343.0
66	89108.0
67	62873.0
68	62873.0
69	62873.0
70	46670.5
71	30468.0
72	30468.0
73	30468.0
74	21642.0
75	12816.0
76	12816.0
77	12816.0
78	8790.5
79	4765.0
80	4765.0
81	4765.0
82	3137.0
83	1509.0
84	1509.0
85	1509.0
86	951.5
87	394.0
88	394.0
89	394.0
90	230.0
91	66.0
92	66.0
93	66.0
94	38.0
95	10.0
96	10.0
97	10.0
98	5.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0848032349989526
2	0.04391897160482536
3	0.019388413568349003
4	0.0062801600471391345
5	0.020062793170726363
6	0.06056771803851635
7	0.07949249563023092
8	0.05357102966385127
9	0.02322394755687022
10	0.04944045459928996
11	0.006533052398030643
12	3.3718980118867834E-4
13	0.005605780444761777
14	9.694206784174503E-4
15	0.013487592047547133
16	0.009272719532688656
17	0.004720657216641497
18	0.005816524070504702
19	0.0018966926316863158
20	0.001433056655051883
21	2.9504107604009356E-4
22	6.32230877228772E-4
23	0.0
24	0.0
25	4.214872514858479E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2372551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.683505005473606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36246964600623	36.67583592511789
2	14.698144293523974	14.899069397439913
3	5.430165289975614	8.256594289650842
4	2.6431916244504596	5.358648637130431
5	1.4060995239026068	3.5633026128955914
6	0.8770250865339061	2.6670423217960293
7	0.550648389679222	1.953615329019398
8	0.3773644133663378	1.5300920906992315
9	0.2819138712322819	1.285954479334242
>10	1.2438148747608155	10.8352110622583
>50	0.07207415868258932	2.546848988184806
>100	0.048581887923541914	4.756784840731773
>500	0.005004082330862837	1.8233905821481224
>1k	0.003252653515060227	3.0167780755454743
>5k	2.502041165430944E-4	0.8308313680479528
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8215	0.34625177709562405	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5936	0.2501948324819993	No Hit
GTCCTACAGTGGACATTTCTAAATT	5504	0.2319865832178107	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4732	0.19944776740310324	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4648	0.1959072744906221	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4625	0.1949378538122047	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4128	0.17398993741335803	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2901	0.12227345165604449	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2598	0.1095023879360233	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2474	0.10427594601759876	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.214872514858479E-5
3	0.0	0.0	0.0	0.0	4.214872514858479E-5
4	0.0	0.0	0.0	0.0	4.214872514858479E-5
5	0.0	0.0	0.0	0.0	4.214872514858479E-5
6	0.0	0.0	0.0	0.0	4.214872514858479E-5
7	0.0	0.0	0.0	0.0	4.214872514858479E-5
8	0.0	0.0	0.0	0.0	4.214872514858479E-5
9	0.0	0.0	0.0	0.0	4.214872514858479E-5
10	0.0	0.0	0.0	0.0	4.214872514858479E-5
11	0.0	0.0	0.0	0.0	4.214872514858479E-5
12	0.0	0.0	0.0	0.0	4.214872514858479E-5
13	0.0	0.0	0.0	0.0	4.214872514858479E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	905	0.0	12.92621	4
TCGGACT	70	1.07990585E-4	12.2281065	4
CGTTTTA	55	0.0030898927	12.080099	17
GGTATCA	2240	0.0	11.721324	1
CCAACGA	140	6.91216E-10	11.524915	19
AGGACCT	1790	0.0	11.157863	5
GGACCTG	1675	0.0	11.128997	6
AATGTCC	690	0.0	10.877633	8
GCGTGCG	140	8.38736E-9	10.854756	9
TCCCGCC	335	0.0	10.767619	13
GGCGAGG	995	0.0	10.492681	19
TAGAAAT	665	0.0	10.440371	4
GTCGAGG	110	6.116363E-6	10.353934	19
ACGGTAC	285	0.0	10.3469925	3
TGTAGGA	2745	0.0	10.326929	2
GTCCTAC	2585	0.0	10.3041725	1
GTATCAA	4600	0.0	10.298813	1
AAATGTC	660	0.0	10.22651	7
CCGTACA	195	1.6370905E-11	10.221622	17
TGTCCAC	800	0.0	10.208748	10
>>END_MODULE