FastQCFastQC Report
Thu 26 May 2016
SRR1512918_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512918_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136864
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46370.4078764038618516No Hit
GTACTGGTTCACTATCGGTCAGTCA28600.2515692290370704No Hit
TATCAACGCAGAGTACTTTTTTTTT26770.23547231682945366No Hit
GGTATCAACGCAGAGTACTTTTTTT25600.22518084836884622No Hit
GGATACCACGTGTCCCGCCCTACTC18740.16483941790750697No Hit
GTACATGGGAAGCAGTGGTATCAAC14680.12912714273651027No Hit
GTCCTACAGTGGACATTTCTAAATT14130.12428927294733583No Hit
ATCATTAACTGAATCCATAGGTTAA12930.11373392068004615No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12770.11232654037774087No Hit
GTCCTAAAGTGTGTATTTCTCATTT12380.10889605089087175No Hit
GTATTTAGCCTTGGAGGATGGTCCC12280.10801643820193092No Hit
CTGTAGGACGTGGAATATGGCAAGA12100.10643313536183747No Hit
GTACATGGGGGTTAAGCGACTAAGC11920.10484983252174404No Hit
CTTTAGGACGTGAAATATGGCGAGG11670.10265080079939201No Hit
CCCATGTACTCTGCGTTGATACCAC11590.10194711064823937No Hit
GTTCACTATCGGTCAGTCAGGAGTA11450.10071565288372224No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGTT400.005282409514.2481847
GTTATAG500.001466542713.33881951
CTTATAC500.001501550713.2971333
TTTGGAC957.4005584E-812.9971993
ACCGTGC853.9462902E-612.2925518
CTTACAC1501.4551915E-1112.0307413
CTAGACA802.8710932E-511.8724424
TAGGACC2500.011.7774634
GTACCAT658.0290844E-411.6908186
TCTTATA907.4734107E-611.6086092
GCCTTAA752.0175069E-411.4332741
GTATTAG752.0175069E-411.4332741
TTAAGAC752.0770315E-411.3975443
GGTATCA13950.011.1327571
TAGACTG1201.277458E-711.0809455
GTACAAG1900.011.0321071
TTAGGCA951.3624405E-510.997634
TTAGGAG1502.1354936E-910.7643473
GTCTAGG803.668815E-410.7186951
TAACCTG803.7765023E-410.6851985
Produced by FastQC (version 0.11.5)