##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512918_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1136864 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.384769858136064 31.0 28.0 33.0 16.0 34.0 2 28.3681917977876 31.0 28.0 34.0 16.0 34.0 3 28.558874236496187 31.0 28.0 34.0 16.0 34.0 4 31.950439102654318 35.0 32.0 37.0 19.0 37.0 5 32.11271620879894 35.0 33.0 37.0 20.0 37.0 6 31.97540339037915 35.0 32.0 37.0 18.0 37.0 7 31.83989729642244 35.0 32.0 37.0 17.0 37.0 8 31.765294705435302 35.0 32.0 37.0 17.0 37.0 9 32.970693064429874 37.0 33.0 39.0 17.0 39.0 10 32.846774108424576 37.0 33.0 39.0 16.0 39.0 11 32.91624943704788 38.0 33.0 39.0 16.0 39.0 12 32.80588003490303 37.0 33.0 39.0 13.0 39.0 13 32.77625292031413 38.0 33.0 39.0 11.0 39.0 14 33.56038189264503 38.0 33.0 41.0 10.0 41.0 15 33.72523450474287 39.0 33.0 41.0 10.0 41.0 16 33.644405135530725 39.0 33.0 41.0 10.0 41.0 17 33.40479951867594 38.0 32.0 41.0 10.0 41.0 18 33.39181555577448 38.0 32.0 41.0 10.0 41.0 19 33.49527296140963 39.0 33.0 41.0 8.0 41.0 20 33.49216265094154 39.0 33.0 41.0 2.0 41.0 21 33.39569112928196 39.0 33.0 41.0 2.0 41.0 22 33.370612491907565 39.0 33.0 41.0 2.0 41.0 23 33.00399080276973 39.0 32.0 41.0 2.0 41.0 24 33.05011681256509 39.0 32.0 41.0 2.0 41.0 25 32.747513334928364 38.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8399796841641063 1101 2 0.5084538877005187 1101 3 0.8807914028120649 1101 4 1.0638306318698483 1101 5 0.7549301343250434 1101 6 0.8798340420030364 1101 7 0.4190950706852945 1101 8 0.8441769814807429 1101 9 1.05058917530517 1101 10 0.8711837396982318 1101 11 0.8914203617323722 1101 12 0.7341838678361157 1101 13 1.2158571958116937 1101 14 1.2077398775816945 1101 15 1.1522027303783702 1101 16 0.9584284673241825 1101 17 0.30483658990337403 1101 18 0.8567989489259631 1101 19 0.33290722564077413 1101 20 -0.27374475009516175 1101 21 0.9422451036902331 1101 22 0.9264094104225222 1101 23 0.5815341575065318 1101 24 0.9677684228965475 1101 25 0.7313869775134307 1102 1 1.2446978878418946 1102 2 1.0879520842828931 1102 3 1.0191273312272244 1102 4 1.2618554953816812 1102 5 1.2554499719189138 1102 6 1.0469876971265144 1102 7 1.1536797918946746 1102 8 1.1451764985984312 1102 9 1.4638834674391177 1102 10 1.3418794351473124 1102 11 1.2783975677161692 1102 12 1.5113201293415273 1102 13 1.303034699408677 1102 14 1.633409260872 1102 15 1.5006836220353463 1102 16 1.4373959860974637 1102 17 1.715757121271018 1102 18 1.7071953558873503 1102 19 1.4798734435882253 1102 20 1.7794622290324966 1102 21 1.8858969258318652 1102 22 1.6477308819324676 1102 23 1.732951022910477 1102 24 0.9885631584938466 1102 25 1.2254458004631488 1103 1 0.6607549130143937 1103 2 0.6249510712665902 1103 3 0.8510144085796227 1103 4 0.7732268423110966 1103 5 0.6170896248174742 1103 6 0.30099914612209844 1103 7 0.3609252476564393 1103 8 0.5574418801997609 1103 9 0.7200105436447046 1103 10 0.8255122931912382 1103 11 0.5345389790581336 1103 12 0.7692582111091468 1103 13 0.9116161898805828 1103 14 0.9423589254298079 1103 15 0.8677801835139647 1103 16 0.927043628445368 1103 17 0.5456970092658224 1103 18 0.8708799159897964 1103 19 0.9518793114373807 1103 20 1.0617079237132074 1103 21 0.9024169759092757 1103 22 0.9579229249761028 1103 23 0.1541878106931165 1103 24 0.45181384180201434 1103 25 0.9778983033779625 1104 1 0.96766451802851 1104 2 0.8699774819469148 1104 3 0.914413856879623 1104 4 1.1218581842131243 1104 5 0.7994925592351763 1104 6 0.8150288126613567 1104 7 1.0670310193226413 1104 8 0.5906486479653736 1104 9 1.0048751745168758 1104 10 1.1155612001528823 1104 11 0.8718922466602663 1104 12 1.2267651073591495 1104 13 1.1176586403864661 1104 14 1.437342738238641 1104 15 1.1108148558787718 1104 16 0.8933015090515894 1104 17 1.2691021219314322 1104 18 1.0306322957141347 1104 19 0.6744670054879549 1104 20 0.8950477810448731 1104 21 0.7852532573223243 1104 22 0.5920072088943726 1104 23 1.045328374458677 1104 24 0.8652342436496099 1104 25 0.5517791306516528 1105 1 0.9860527230554084 1105 2 0.9912407457284154 1105 3 1.0700499847750606 1105 4 1.220929236982144 1105 5 0.9440084553365935 1105 6 1.1332744171355635 1105 7 1.08960414184331 1105 8 1.1010541739638668 1105 9 0.692999477692922 1105 10 1.0380039839518247 1105 11 0.9771362230357994 1105 12 1.1613720816161148 1105 13 1.223338025296691 1105 14 1.334738461584486 1105 15 1.2378604812435015 1105 16 1.1862332447137618 1105 17 1.6710699187301898 1105 18 1.4365988118327593 1105 19 1.1414191003309497 1105 20 1.4342004885252706 1105 21 1.2123038782478233 1105 22 1.1853833223935126 1105 23 1.5550191946616323 1105 24 1.6162838790872058 1105 25 1.965908847198989 1106 1 0.8335304213798729 1106 2 0.6944697688267674 1106 3 0.6330277421342316 1106 4 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1107 17 1.4515356204010317 1107 18 1.3573981192692628 1107 19 1.1113001307105321 1107 20 1.097072240445243 1107 21 1.0489998370182292 1107 22 1.1141439579488548 1107 23 1.4415201729413454 1107 24 1.2895311373147749 1107 25 1.5046781060254588 1108 1 0.832094797446679 1108 2 0.7628837434590992 1108 3 0.843125385251227 1108 4 1.019733802382568 1108 5 0.9459922968969039 1108 6 1.0251383249354369 1108 7 1.0490569405765058 1108 8 1.0923276129013573 1108 9 1.1703497835212175 1108 10 1.1814817842183416 1108 11 1.0357766392076257 1108 12 1.2426092226093743 1108 13 1.2139518564304055 1108 14 1.3400944992540573 1108 15 1.44121204718477 1108 16 1.0891855890256892 1108 17 1.2880675044429566 1108 18 1.3755993080719406 1108 19 1.3053112881881432 1108 20 1.4751469114214117 1108 21 1.4454721702371813 1108 22 1.4266280463674406 1108 23 1.8167362937254694 1108 24 1.5846115530636453 1108 25 1.8075410837879886 1109 1 0.5029256549867291 1109 2 0.5509573333780402 1109 3 0.6514916980195942 1109 4 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-4.8600252237372 2201 1 1.3149649502704754 2201 2 1.1652499203175566 2201 3 1.239424690707832 2201 4 1.4309895889809994 2201 5 1.311441870500012 2201 6 1.3873734753187854 2201 7 1.3228340621776837 2201 8 1.5050391818539595 2201 9 1.6926809755225989 2201 10 1.6494973182224726 2201 11 1.648794401450047 2201 12 1.5300098234758153 2201 13 1.6007458307889877 2201 14 1.8918690561074811 2201 15 1.625533738099314 2201 16 1.6710246889993172 2201 17 1.9531111445658027 2201 18 1.3811353433842086 2201 19 1.3135900271568488 2201 20 1.1458326934421095 2201 21 1.5947035038150688 2201 22 1.6667927237882623 2201 23 1.8745974274894976 2201 24 1.811901560551739 2201 25 0.8648638024206079 2202 1 0.9972849770718959 2202 2 1.025764279900308 2202 3 1.1117703226797708 2202 4 1.3273283529189115 2202 5 1.1217553728054313 2202 6 1.1241081531990638 2202 7 1.080144213964914 2202 8 0.9058754651951304 2202 9 0.7604234024347178 2202 10 1.3034611438046753 2202 11 1.2742674052572553 2202 12 1.3315426574293667 2202 13 1.5377699939859113 2202 14 1.5995785197280625 2202 15 1.298946655469905 2202 16 1.2219458514529293 2202 17 1.675662609663462 2202 18 0.9156512018560861 2202 19 1.3040496898965728 2202 20 1.2786278626431056 2202 21 1.0841971362627802 2202 22 1.2000765485661447 2202 23 0.7012180800549714 2202 24 0.9872456864775074 2202 25 1.0040444165152778 2203 1 1.192403933110981 2203 2 1.3325437418518078 2203 3 1.257135904078858 2203 4 1.5037552448370732 2203 5 1.1869570882913507 2203 6 1.097054483866767 2203 7 1.1693897689029242 2203 8 1.2124892944850743 2203 9 1.2218164366945814 2203 10 1.2148610543295746 2203 11 1.2119041985955405 2203 12 1.2374382098075998 2203 13 1.1755015827444382 2203 14 1.7158610134264975 2203 15 1.5163785059949575 2203 16 1.4175429672361801 2203 17 1.6827530260238177 2203 18 0.9736684751677132 2203 19 1.3224158424773762 2203 20 1.6196447668783165 2203 21 1.1826026793020077 2203 22 1.4538150785043342 2203 23 1.617436060668254 2203 24 1.29583907703163 2203 25 0.9602310641135574 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1.0389418782649784 2205 14 1.3834067806148411 2205 15 1.211589808788787 2205 16 1.25664972632525 2205 17 1.3771772173065742 2205 18 1.2371146979247314 2205 19 1.1559369778554611 2205 20 1.350583602896542 2205 21 1.437692135959253 2205 22 1.1477383227039297 2205 23 1.8047619082630746 2205 24 1.5346984217979411 2205 25 1.9375201883356823 2206 1 0.6799555020364494 2206 2 0.8169790737192564 2206 3 0.9070423225833579 2206 4 0.7444854227536055 2206 5 0.7398193858499837 2206 6 0.8145025484177104 2206 7 0.708129424836379 2206 8 0.6226439800251313 2206 9 0.7772815236707871 2206 10 0.7962527302484688 2206 11 0.5003579503970954 2206 12 0.3880155395212128 2206 13 0.7235949836523474 2206 14 0.9898240269488596 2206 15 0.8250300672753426 2206 16 0.8280884015320282 2206 17 0.6432606596248149 2206 18 1.0785146721157801 2206 19 0.9346810746787284 2206 20 1.009744477025798 2206 21 0.902001026894105 2206 22 0.775498205173939 2206 23 0.8295685893066462 2206 24 0.8584512167334353 2206 25 1.0781330516152607 2207 1 0.8546107918914778 2207 2 0.8360711849871265 2207 3 0.917448272560847 2207 4 0.9869796200023089 2207 5 0.7080211072356235 2207 6 0.9140221131637638 2207 7 0.842549337041163 2207 8 1.04512235521792 2207 9 1.229622493889572 2207 10 1.299324088580569 2207 11 1.0999816237797404 2207 12 0.9542344673926593 2207 13 0.8378374750856494 2207 14 1.2256672485552969 2207 15 0.9627059079909728 2207 16 1.0849471337897967 2207 17 1.3494937266161102 2207 18 1.2848002540830592 2207 19 1.088100045299207 2207 20 1.098396819900067 2207 21 1.2191936315570686 2207 22 0.6523109699369485 2207 23 1.0922893441464723 2207 24 1.1469018529631185 2207 25 1.1509925135547974 2208 1 0.5257875107339096 2208 2 0.9527670063845797 2208 3 0.9528099637895409 2208 4 0.9575418188805642 2208 5 0.01628561057887623 2208 6 0.7734015503291083 2208 7 0.9194942118937988 2208 8 1.20759140669918 2208 9 0.9080966751502544 2208 10 1.0061927978343377 2208 11 1.039957694497069 2208 12 1.1857799466534402 2208 13 0.9054292216629207 2208 14 1.0381899168882995 2208 15 0.95384747161701 2208 16 1.0241227360938367 2208 17 1.3895310737354265 2208 18 1.5875280833180838 2208 19 1.2644153232863857 2208 20 1.2306348678228147 2208 21 1.022011074502501 2208 22 1.0705956787499105 2208 23 1.3965088178112381 2208 24 1.2470503210833428 2208 25 1.3748475621672114 2209 1 0.4416005427091534 2209 2 0.5774432347253722 2209 3 0.34656095954580834 2209 4 0.04116965467371969 2209 5 0.010980945370242523 2209 6 0.5005275201353676 2209 7 0.15976386269113263 2209 8 0.07382694107630527 2209 9 0.08888346743911768 2209 10 0.46853872718271106 2209 11 0.3691055323179384 2209 12 0.43443959836807267 2209 13 0.22228248701929232 2209 14 0.5139402343233215 2209 15 0.4177190202654373 2209 16 0.40365704804436575 2209 17 0.39883234250995514 2209 18 0.529098010754609 2209 19 0.38695308960592456 2209 20 0.36519231752807 2209 21 0.39585267804424973 2209 22 0.2931954837023767 2209 23 0.7086147397246378 2209 24 0.35305430893632206 2209 25 0.3930342960383726 2210 1 -0.22416743034416697 2210 2 -0.08780450129149031 2210 3 -0.08679342076404595 2210 4 -0.23465424846760286 2210 5 -0.10668844184335313 2210 6 -0.12053292809152794 2210 7 -0.3958852236080226 2210 8 -0.29208825844411734 2210 9 -0.24118482058179325 2210 10 -0.24314676711003358 2210 11 -0.11717329262199172 2210 12 -0.1381411458595636 2210 13 -0.3007037595595463 2210 14 -0.4819209023116784 2210 15 -0.09584153851950816 2210 16 -0.0435210806255526 2210 17 0.03613038980914496 2210 18 0.008038238160224864 2210 19 -0.17261865765346585 2210 20 -0.09458543622721294 2210 21 -0.0604102511052389 2210 22 -0.059582348671966656 2210 23 0.34212126019262 2210 24 0.06197462360909611 2210 25 0.2602270552232895 2211 1 0.12097616420193447 2211 2 0.4674074692211576 2211 3 0.29248046904746516 2211 4 0.19249034245073915 2211 5 -0.2039206401546636 2211 6 0.24590099045112268 2211 7 -0.05922364749010711 2211 8 -0.13037497474546456 2211 9 -0.25351876768628046 2211 10 0.08549554873745535 2211 11 -0.054155099893250735 2211 12 0.008202359270121917 2211 13 0.06996866002289437 2211 14 0.4761731485810543 2211 15 -0.0027161620974140988 2211 16 -0.1287789413221745 2211 17 0.4119508371357625 2211 18 -0.12225796781856957 2211 19 0.1625292352763381 2211 20 0.276605548391089 2211 21 0.35608557231427795 2211 22 0.07144373602719867 2211 23 0.4098306794217237 2211 24 0.0579283114247886 2211 25 0.4410397726702442 2212 1 0.3662960616957456 2212 2 0.4212434882745697 2212 3 0.11805344679435592 2212 4 0.4968454076753659 2212 5 0.3962400687017009 2212 6 0.22624673166642495 2212 7 0.47891212977610564 2212 8 0.5587619752610493 2212 9 0.5722134710894409 2212 10 0.2859907057543509 2212 11 0.09565161513085485 2212 12 0.492725082923279 2212 13 0.5518273810120533 2212 14 0.4525735597640761 2212 15 0.2567128147228672 2212 16 0.1557553649512613 2212 17 0.2413895144005238 2212 18 0.6590549221899735 2212 19 0.4167315546960495 2212 20 0.3539774518499499 2212 21 0.5578041435980623 2212 22 0.30506518703995766 2212 23 0.4259449998838676 2212 24 0.4241418080730739 2212 25 0.5845773944687309 2213 1 -0.3645210009753015 2213 2 -0.1393085731553505 2213 3 -0.33670007510296074 2213 4 -0.21890335541666062 2213 5 -0.23059137730358614 2213 6 -0.120996931544461 2213 7 -0.4204861261414514 2213 8 -0.0749056811820914 2213 9 -0.5188662856368822 2213 10 -0.281051800862393 2213 11 -0.44394663440910875 2213 12 -0.2964672207048764 2213 13 -0.35495136861826637 2213 14 -0.3996602525760551 2213 15 -0.22598454189240869 2213 16 -0.31889685335478646 2213 17 -0.2629459941901473 2213 18 -0.23540132912697231 2213 19 -0.3160128522540191 2213 20 -0.2059552958708437 2213 21 -0.47949692414753997 2213 22 -0.5054442008305031 2213 23 -0.2565618419538467 2213 24 0.06046053985865285 2213 25 0.18795088784198555 2214 1 -3.079152096882389 2214 2 -1.6230922567124608 2214 3 -1.8551988544529578 2214 4 -3.4396567355456824 2214 5 -1.9310347756693353 2214 6 -1.6849992589674585 2214 7 -1.5624359565894075 2214 8 -1.2863096600515078 2214 9 -1.1407283093594032 2214 10 -1.2554278319498344 2214 11 -1.1469045159947377 2214 12 -1.0646470951907503 2214 13 -1.5276903273885516 2214 14 -1.3665282769936482 2214 15 -1.8946887429040444 2214 16 -1.5057647661925984 2214 17 -3.8173672282165967 2214 18 -3.7706124626889554 2214 19 -2.3961350124254857 2214 20 -2.0585236163358545 2214 21 -2.0889942670719215 2214 22 -3.0522809632184007 2214 23 -2.0720774701792024 2214 24 -3.120340058208985 2214 25 -3.109966475514625 2215 1 -2.129638196268715 2215 2 -3.9098886130643606 2215 3 -2.4834540109114265 2215 4 -4.258941562020993 2215 5 -2.476385740584604 2215 6 -2.5596071343496654 2215 7 -2.4518639223713237 2215 8 -1.7315438431739238 2215 9 -4.547064011206746 2215 10 -2.75385617436795 2215 11 -2.237727194057136 2215 12 -2.2417731679282227 2215 13 -2.3722543578241293 2215 14 -1.9617623021605297 2215 15 -2.2880039944236614 2215 16 -2.42562233944097 2215 17 -2.7300473019151426 2215 18 -2.4623699567138466 2215 19 -2.5080858229655405 2215 20 -2.2973685331336533 2215 21 -3.174021732029484 2215 22 -2.9254826833190855 2215 23 -2.8201984135739053 2215 24 -2.4870405039595056 2215 25 -3.73998511777798 2216 1 -3.8841145331441993 2216 2 -3.9900083034120044 2216 3 -5.201446573113273 2216 4 -5.750720884727883 2216 5 -5.746360763437178 2216 6 -4.668568824154562 2216 7 -6.214485505197619 2216 8 -4.020258868026094 2216 9 -4.86798152413273 2216 10 -3.654861241211723 2216 11 -4.117257984327654 2216 12 -4.641571779634454 2216 13 -4.094339405100385 2216 14 -5.266703992393911 2216 15 -4.910307212351505 2216 16 -4.955257037501358 2216 17 -4.832987874514906 2216 18 -4.126928116510459 2216 19 -4.9073671274189365 2216 20 -5.753506323432735 2216 21 -5.3124279793514475 2216 22 -4.93243531065076 2216 23 -5.137729550203723 2216 24 -5.434872366158494 2216 25 -5.719553936157162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67730.0 3 0.0 4 588.0 5 3747.0 6 4800.0 7 4433.0 8 3817.0 9 3524.0 10 3061.0 11 2862.0 12 2610.0 13 2508.0 14 2569.0 15 2599.0 16 2695.0 17 2778.0 18 3237.0 19 3962.0 20 5105.0 21 6610.0 22 8374.0 23 9824.0 24 11395.0 25 12926.0 26 14160.0 27 15507.0 28 17085.0 29 20333.0 30 24174.0 31 30229.0 32 37176.0 33 46313.0 34 58781.0 35 75462.0 36 101301.0 37 152457.0 38 372132.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.19604887232313 17.812996196799098 12.07368447995707 23.917270450920704 2 16.223153916664764 19.911010083060773 39.701589960220204 24.16424604005426 3 19.880943584113613 25.468871506335482 28.240579465363325 26.409605444187576 4 12.874289424097036 16.149932175299142 36.67706469473607 34.29871370586776 5 13.966430318278586 38.43279905695234 33.220789274592256 14.379981350176822 6 32.60371026710296 35.000426963304925 18.180537692056458 14.215325077535656 7 29.495438931331307 31.40702057718955 20.80361434540169 18.293926146077446 8 27.059105306119573 34.312742206892786 20.400457534929306 18.227694952058336 9 28.633318748497867 14.176107926608639 19.183049662685328 38.00752366220817 10 17.551558009804474 27.706883557784412 31.76910182002592 22.97245661238519 11 35.5321565347537 22.366421622573405 23.044239996478407 19.057181846194478 12 24.76187121939193 24.57776055928786 29.528144893592668 21.13222332772754 13 30.0214634592969 21.336597751178818 25.02561881318867 23.61631997633561 14 22.721362019222727 21.90822147074904 26.49001666603571 28.88039984399252 15 25.307299654359422 28.836953784878798 22.824515654190385 23.0312309065714 16 23.558438951553548 27.301884400432453 25.874409694220724 23.265266953793276 17 22.893155413109803 27.37491360068327 26.15920367345681 23.572727312750118 18 22.762181667051166 26.123333820558926 28.251042105096317 22.86344240729359 19 24.318913007876677 26.136729831531014 26.976873594550955 22.567483566041357 20 25.37364288940523 25.367831889534653 26.897621628189338 22.360903592870784 21 25.195327459057165 25.44543464156146 26.14601646428908 23.213221435092297 22 25.122811342934 26.437788647380216 25.769293422031637 22.670106587654146 23 24.269745229659577 26.062805079910685 26.57699459748822 23.090455092941518 24 24.273623470143306 25.746758477877883 26.776861250439115 23.202756801539696 25 24.132848865618318 25.906109875094312 26.849131506219752 23.111909753067618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 428.0 3 672.0 4 672.0 5 672.0 6 1182.0 7 1692.0 8 1692.0 9 1692.0 10 1869.0 11 2046.0 12 2046.0 13 2046.0 14 2565.5 15 3085.0 16 3085.0 17 3085.0 18 6078.0 19 9071.0 20 9071.0 21 9071.0 22 14506.0 23 19941.0 24 19941.0 25 19941.0 26 31278.5 27 42616.0 28 42616.0 29 42616.0 30 54113.0 31 65610.0 32 65610.0 33 65610.0 34 75882.5 35 86155.0 36 86155.0 37 86155.0 38 94652.0 39 103149.0 40 103149.0 41 103149.0 42 114299.5 43 125450.0 44 125450.0 45 125450.0 46 143401.5 47 161353.0 48 161353.0 49 161353.0 50 159310.0 51 157267.0 52 157267.0 53 157267.0 54 141678.5 55 126090.0 56 126090.0 57 126090.0 58 110847.0 59 95604.0 60 95604.0 61 95604.0 62 81482.5 63 67361.0 64 67361.0 65 67361.0 66 52486.0 67 37611.0 68 37611.0 69 37611.0 70 28289.0 71 18967.0 72 18967.0 73 18967.0 74 13567.5 75 8168.0 76 8168.0 77 8168.0 78 5690.0 79 3212.0 80 3212.0 81 3212.0 82 2168.5 83 1125.0 84 1125.0 85 1125.0 86 725.5 87 326.0 88 326.0 89 326.0 90 203.0 91 80.0 92 80.0 93 80.0 94 53.0 95 26.0 96 26.0 97 26.0 98 14.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.34049807188898584 2 0.009323894502772538 3 0.008092436738255411 4 0.009675739578348863 5 0.01090719734286599 6 0.08224378641596532 7 0.08321136037380021 8 0.10669701916851972 9 0.08629000478509302 10 0.09288709995214907 11 0.08919272665859769 12 0.09974807892588736 13 0.08620204351619895 14 0.08980845554085626 15 0.08813719143186872 16 0.08901680412080953 17 0.10106749795929855 18 0.10397021983280322 19 0.0972851633968531 20 0.09561389928786557 21 0.08365116671827061 22 0.10370633602612098 23 0.09640555070791229 24 0.09279913868325498 25 0.09209544853210234 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1136864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.08569944100267 #Duplication Level Percentage of deduplicated Percentage of total 1 76.14063795101187 41.942602974156415 2 13.322403834836575 14.677478669549377 3 4.656783552975148 7.695665374829806 4 2.1095607839986945 4.648265251995121 5 1.1208523299441986 3.0871467282526837 6 0.6732494691376024 2.2251850743437145 7 0.4201936642479181 1.620266332704208 8 0.29319139618427387 1.2920522503115877 9 0.2165881408492412 1.0737818306376168 >10 0.919419449733807 8.744044436417028 >50 0.06826714961396517 2.618839570809403 >100 0.05028313121116979 5.358516929467535 >500 0.005173824154251914 2.0098106243474847 >1k 0.0033953221012278188 3.0063439521780264 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4637 0.4078764038618516 No Hit GTACTGGTTCACTATCGGTCAGTCA 2860 0.2515692290370704 No Hit TATCAACGCAGAGTACTTTTTTTTT 2677 0.23547231682945366 No Hit GGTATCAACGCAGAGTACTTTTTTT 2560 0.22518084836884622 No Hit GGATACCACGTGTCCCGCCCTACTC 1874 0.16483941790750697 No Hit GTACATGGGAAGCAGTGGTATCAAC 1468 0.12912714273651027 No Hit GTCCTACAGTGGACATTTCTAAATT 1413 0.12428927294733583 No Hit ATCATTAACTGAATCCATAGGTTAA 1293 0.11373392068004615 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1277 0.11232654037774087 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1238 0.10889605089087175 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1228 0.10801643820193092 No Hit CTGTAGGACGTGGAATATGGCAAGA 1210 0.10643313536183747 No Hit GTACATGGGGGTTAAGCGACTAAGC 1192 0.10484983252174404 No Hit CTTTAGGACGTGAAATATGGCGAGG 1167 0.10265080079939201 No Hit CCCATGTACTCTGCGTTGATACCAC 1159 0.10194711064823937 No Hit GTTCACTATCGGTCAGTCAGGAGTA 1145 0.10071565288372224 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 8.796126889408055E-5 7 0.0 0.0 0.0 0.0 8.796126889408055E-5 8 0.0 0.0 0.0 0.0 8.796126889408055E-5 9 0.0 0.0 0.0 0.0 8.796126889408055E-5 10 0.0 0.0 0.0 0.0 8.796126889408055E-5 11 0.0 0.0 0.0 0.0 8.796126889408055E-5 12 0.0 0.0 0.0 0.0 1.759225377881611E-4 13 0.0 0.0 0.0 0.0 3.518450755763222E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACCGTT 40 0.0052824095 14.248184 7 GTTATAG 50 0.0014665427 13.3388195 1 CTTATAC 50 0.0015015507 13.297133 3 TTTGGAC 95 7.4005584E-8 12.997199 3 ACCGTGC 85 3.9462902E-6 12.292551 8 CTTACAC 150 1.4551915E-11 12.030741 3 CTAGACA 80 2.8710932E-5 11.872442 4 TAGGACC 250 0.0 11.777463 4 GTACCAT 65 8.0290844E-4 11.690818 6 TCTTATA 90 7.4734107E-6 11.608609 2 GCCTTAA 75 2.0175069E-4 11.433274 1 GTATTAG 75 2.0175069E-4 11.433274 1 TTAAGAC 75 2.0770315E-4 11.397544 3 GGTATCA 1395 0.0 11.132757 1 TAGACTG 120 1.277458E-7 11.080945 5 GTACAAG 190 0.0 11.032107 1 TTAGGCA 95 1.3624405E-5 10.99763 4 TTAGGAG 150 2.1354936E-9 10.764347 3 GTCTAGG 80 3.668815E-4 10.718695 1 TAACCTG 80 3.7765023E-4 10.685198 5 >>END_MODULE