FastQCFastQC Report
Thu 26 May 2016
SRR1512918_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512918_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1136864
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61340.5395544233962901No Hit
TATCAACGCAGAGTACTTTTTTTTT43980.38685366059616627No Hit
GGTATCAACGCAGAGTACTTTTTTT35870.31551707152306696No Hit
GTACTGGTTCACTATCGGTCAGTCA27240.23960649646747545No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21130.18586216117319224No Hit
GGATACCACGTGTCCCGCCCTACTC18080.15903397416049764No Hit
ATCATTAACTGAATCCATAGGTTAA14860.1307104455766037No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14000.12314577645171278No Hit
GTACATGGGAAGCAGTGGTATCAAC13970.12288189264503054No Hit
CCCATGTACTCTGCGTTGATACCAC13890.1221782024938779No Hit
GTCCTACAGTGGACATTTCTAAATT13700.12050693838489036No Hit
CCCATATTCAGACAGGATACCACGT13410.11795606158696204No Hit
TATTCAGACAGGATACCACGTGTCC12760.1122385791088468No Hit
CTATCGGTCAGTCAGGAGTATTTAG12650.11127100515101192No Hit
CTTTAGGACGTGAAATATGGCGAGG12320.10836828327750725No Hit
CTGTAGGACGTGGAATATGGCAAGA12180.10713682551299011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC1300.015.3676581
GTCTTAC801.9668787E-613.0808031
AAAACGC604.118263E-412.65652218
TAATACC1001.4215402E-712.36294654
GTAAGAC853.8871112E-612.3091743
AAGGCGA701.0985896E-412.20450317
CCGTATA550.00303556612.1078511
CTATCCC550.003043593412.1035834
CCCTATA657.938563E-411.7066282
CCTAATA657.938563E-411.7066282
CTATACT1302.568413E-911.7045644
CCTATAC907.3546E-611.6253313
TAAGACC1406.675691E-1011.5478064
TAGGACC2400.011.49119954
GGTATCA13900.011.4296621
TTAGGCA752.0528036E-411.411954
TAGGGCG752.0528036E-411.411954
TATGGGT600.005828374511.0949514
GACGTGG3350.010.7821887
TCAGTAC1158.668958E-710.7522833
Produced by FastQC (version 0.11.5)