##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512918_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1136864 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.27409874883891 31.0 30.0 34.0 26.0 34.0 2 30.23902859093084 31.0 30.0 34.0 26.0 34.0 3 30.431702472767192 31.0 30.0 34.0 26.0 34.0 4 34.051044803951925 37.0 35.0 37.0 30.0 37.0 5 33.659384939623386 37.0 35.0 37.0 28.0 37.0 6 33.52706744166409 37.0 35.0 37.0 28.0 37.0 7 33.35679553578968 37.0 35.0 37.0 27.0 37.0 8 33.30580350859909 37.0 35.0 37.0 27.0 37.0 9 34.61188761364596 39.0 35.0 39.0 27.0 39.0 10 34.53628226419343 39.0 35.0 39.0 27.0 39.0 11 34.5332863033749 39.0 35.0 39.0 27.0 39.0 12 34.415071635657384 39.0 35.0 39.0 26.0 39.0 13 34.39896944577364 39.0 35.0 39.0 26.0 39.0 14 35.453293445829935 39.0 36.0 41.0 26.0 41.0 15 35.44957180454303 39.0 36.0 41.0 26.0 41.0 16 35.447609388634 39.0 36.0 41.0 26.0 41.0 17 35.44197195091058 40.0 36.0 41.0 26.0 41.0 18 35.37124229459285 40.0 35.0 41.0 26.0 41.0 19 35.37641089875306 40.0 35.0 41.0 25.0 41.0 20 35.35614902046331 40.0 35.0 41.0 25.0 41.0 21 35.32786155599966 40.0 35.0 41.0 25.0 41.0 22 35.283510604610576 40.0 35.0 41.0 25.0 41.0 23 35.13344252258845 40.0 35.0 41.0 25.0 41.0 24 35.08144861654516 40.0 35.0 41.0 25.0 41.0 25 35.00916996228221 39.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.762905994093888 1101 2 0.6232670237856013 1101 3 0.5211377116281 1101 4 0.5444037409329852 1101 5 0.7548531919158137 1101 6 0.701929554461465 1101 7 0.6332116543196946 1101 8 0.4949221692187038 1101 9 0.6295687835270485 1101 10 0.35981986092595264 1101 11 0.9809151914620742 1101 12 0.8675631481258321 1101 13 0.9743709740593545 1101 14 1.0693215990136125 1101 15 0.6551850378401269 1101 16 0.8733005979221744 1101 17 1.0090314265387192 1101 18 0.7730537834832916 1101 19 0.6234734496760694 1101 20 0.7057306987794334 1101 21 0.7069974308960596 1101 22 0.6822451322568881 1101 23 0.7275343895267383 1101 24 0.7577096816319155 1101 25 0.8364991777034732 1102 1 0.8410516274580999 1102 2 0.7887416773914495 1102 3 0.6246642307364425 1102 4 0.743742980541164 1102 5 0.783576066740892 1102 6 0.7817320161757948 1102 7 0.8079103456100754 1102 8 0.8971495870794399 1102 9 0.9520848960287722 1102 10 0.9428201861324084 1102 11 0.945588408246671 1102 12 1.0765243105910898 1102 13 1.0676892132142086 1102 14 1.042818504624904 1102 15 1.1678951901944288 1102 16 1.3055430935368122 1102 17 1.2179383879278447 1102 18 0.6217884347240101 1102 19 0.9720886121807482 1102 20 1.3001194091880137 1102 21 1.2301326675083928 1102 22 1.0145001926664534 1102 23 1.223559233329091 1102 24 1.2406560521307597 1102 25 1.28676106744863 1103 1 0.6993750854752605 1103 2 0.860937007959695 1103 3 0.5618978361188063 1103 4 0.41979517955108747 1103 5 0.588012552529996 1103 6 0.48108000638967496 1103 7 0.6461658072970309 1103 8 0.7373952435331148 1103 9 0.8910028799351863 1103 10 0.8909401329968318 1103 11 0.9254451524505001 1103 12 1.0272508630863655 1103 13 0.9720439887259715 1103 14 1.0358719364565516 1103 15 1.127056775851642 1103 16 1.0073758407223536 1103 17 1.0986377992315752 1103 18 0.9154532601220424 1103 19 0.9334351115404473 1103 20 0.928222966213184 1103 21 0.9320872633531181 1103 22 0.7664856090543779 1103 23 0.912808119966833 1103 24 0.9886921384473908 1103 25 1.0661660159377035 1104 1 0.8518800705775185 1104 2 0.9620309673512573 1104 3 0.8819264342573945 1104 4 0.7576423522037103 1104 5 0.9280264601593871 1104 6 0.8280235515457193 1104 7 0.6964854064625712 1104 8 0.8176910799941339 1104 9 0.7822309420313758 1104 10 0.7935959375900339 1104 11 1.0376997541719106 1104 12 0.9349113419324908 1104 13 0.9046104068666807 1104 14 1.175513515663262 1104 15 1.0922140195621282 1104 16 1.0445672364128242 1104 17 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-3.991073348083681 2216 22 -3.095790051749894 2216 23 -4.607252239490126 2216 24 -3.5564954965418067 2216 25 -3.7615427673596322 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29337.0 3 1.0 4 450.0 5 4175.0 6 5324.0 7 4209.0 8 3447.0 9 2819.0 10 2389.0 11 2178.0 12 1941.0 13 1842.0 14 1762.0 15 1825.0 16 1859.0 17 2059.0 18 2063.0 19 2840.0 20 3710.0 21 4759.0 22 6090.0 23 7504.0 24 8857.0 25 10124.0 26 11142.0 27 12249.0 28 13932.0 29 16957.0 30 20769.0 31 26612.0 32 34127.0 33 43310.0 34 56128.0 35 72936.0 36 99699.0 37 152449.0 38 464990.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81269456691694 18.84666718316406 13.11838824639743 26.222250003521573 2 14.51433076880432 20.51001363075182 38.61911420352499 26.35654139691887 3 17.818385924412073 25.512399013603854 29.01845137916867 27.6507636828154 4 12.141844271091735 16.808116847701786 35.876445729928676 35.17359315127781 5 13.588299621606357 38.32553114555354 33.585889022713296 14.500280210126803 6 32.64423237727067 35.54753995623694 17.71717374427656 14.091053922215826 7 28.899514623785016 31.54927792145602 20.882268539628495 18.668938915130465 8 27.03168907793029 34.643685990606016 20.07447245742388 18.250152474039812 9 27.77504550769342 13.975810758379442 19.303972036413196 38.94517169751395 10 16.803007807308823 28.316126039940396 31.87654401391252 23.00432213883826 11 35.25358573885353 22.26311692081686 23.048218896107038 19.435078444222572 12 24.12401186074966 24.87713955732417 29.785663833421733 21.213184748504432 13 29.916386268170925 21.090805128543465 25.244309559939303 23.748499043346307 14 22.414771302886123 21.752680425701477 26.85861760491462 28.973930666497775 15 25.209777228811575 28.73072679957983 22.882565220527816 23.176930751080775 16 23.41592925025006 27.1317338456761 25.928203395854283 23.524133508219567 17 22.483446708826573 27.428013965726088 26.510424592908233 23.578114732539106 18 22.535585443330277 25.801678614250363 28.756187481911482 22.906548460507878 19 24.120738717579947 25.926453707002334 27.36720808208755 22.585599493330164 20 25.022936203488765 25.12127819965536 27.40821340389093 22.44757219296494 21 24.732707459034202 25.12439933395669 26.805340661099887 23.337552545909226 22 24.594782642350545 26.20536055922502 26.477513922131063 22.722342876293368 23 23.698788949249867 25.868881414135732 27.228674669969323 23.203654966645086 24 24.048171109297154 25.49231922200017 27.356834238747997 23.102675429954683 25 23.649882483744758 25.807836293523234 27.326047794635066 23.216233428096942 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 91.0 1 91.0 2 252.0 3 413.0 4 413.0 5 413.0 6 1019.5 7 1626.0 8 1626.0 9 1626.0 10 1742.5 11 1859.0 12 1859.0 13 1859.0 14 2345.5 15 2832.0 16 2832.0 17 2832.0 18 6013.5 19 9195.0 20 9195.0 21 9195.0 22 15253.5 23 21312.0 24 21312.0 25 21312.0 26 33408.0 27 45504.0 28 45504.0 29 45504.0 30 56475.5 31 67447.0 32 67447.0 33 67447.0 34 77892.5 35 88338.0 36 88338.0 37 88338.0 38 96297.5 39 104257.0 40 104257.0 41 104257.0 42 115349.5 43 126442.0 44 126442.0 45 126442.0 46 143400.0 47 160358.0 48 160358.0 49 160358.0 50 158314.0 51 156270.0 52 156270.0 53 156270.0 54 140737.0 55 125204.0 56 125204.0 57 125204.0 58 109934.5 59 94665.0 60 94665.0 61 94665.0 62 80080.5 63 65496.0 64 65496.0 65 65496.0 66 50535.0 67 35574.0 68 35574.0 69 35574.0 70 26800.0 71 18026.0 72 18026.0 73 18026.0 74 12843.5 75 7661.0 76 7661.0 77 7661.0 78 5303.5 79 2946.0 80 2946.0 81 2946.0 82 1958.5 83 971.0 84 971.0 85 971.0 86 635.5 87 300.0 88 300.0 89 300.0 90 183.5 91 67.0 92 67.0 93 67.0 94 38.5 95 10.0 96 10.0 97 10.0 98 5.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0886649590452332 2 0.0407260674979593 3 0.017680215047710193 4 0.005981366284797478 5 0.01908759535001548 6 0.0656191065949841 7 0.07371154333323951 8 0.05321656768091874 9 0.021374588341261574 10 0.05462394798322402 11 0.006069327553691559 12 4.398063444704028E-4 13 0.00782855293157317 14 9.675739578348862E-4 15 0.01600895093872266 16 0.01099515861176007 17 0.007124862780420525 18 0.008180398007149493 19 0.002550876797928336 20 0.0013194190334112083 21 8.796126889408057E-5 22 4.398063444704028E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1136864.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.54841743131815 #Duplication Level Percentage of deduplicated Percentage of total 1 74.02759220602188 38.900328166774315 2 14.367918523114634 15.10022760343592 3 5.087205133646712 8.019737367648364 4 2.340531483254301 4.91964901572757 5 1.2371703665302771 3.250567242704494 6 0.725012848112265 2.28589666714033 7 0.49873300341318527 1.8345341035091758 8 0.32430265663610447 1.3633273099999568 9 0.22972040075595904 1.0864299160272446 >10 1.0222472739972137 9.203465008763684 >50 0.07290765773125317 2.6391372979272183 >100 0.057423653530983075 5.741937395786654 >500 0.00520506528933467 1.887185579115735 >1k 0.003861822634022497 3.226364723527285 >5k 1.6790533191402162E-4 0.541212601912007 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6134 0.5395544233962901 No Hit TATCAACGCAGAGTACTTTTTTTTT 4398 0.38685366059616627 No Hit GGTATCAACGCAGAGTACTTTTTTT 3587 0.31551707152306696 No Hit GTACTGGTTCACTATCGGTCAGTCA 2724 0.23960649646747545 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2113 0.18586216117319224 No Hit GGATACCACGTGTCCCGCCCTACTC 1808 0.15903397416049764 No Hit ATCATTAACTGAATCCATAGGTTAA 1486 0.1307104455766037 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1400 0.12314577645171278 No Hit GTACATGGGAAGCAGTGGTATCAAC 1397 0.12288189264503054 No Hit CCCATGTACTCTGCGTTGATACCAC 1389 0.1221782024938779 No Hit GTCCTACAGTGGACATTTCTAAATT 1370 0.12050693838489036 No Hit CCCATATTCAGACAGGATACCACGT 1341 0.11795606158696204 No Hit TATTCAGACAGGATACCACGTGTCC 1276 0.1122385791088468 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1265 0.11127100515101192 No Hit CTTTAGGACGTGAAATATGGCGAGG 1232 0.10836828327750725 No Hit CTGTAGGACGTGGAATATGGCAAGA 1218 0.10713682551299011 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.759225377881611E-4 13 0.0 0.0 0.0 0.0 1.759225377881611E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 130 0.0 15.367658 1 GTCTTAC 80 1.9668787E-6 13.080803 1 AAAACGC 60 4.118263E-4 12.656522 18 TAATACC 100 1.4215402E-7 12.3629465 4 GTAAGAC 85 3.8871112E-6 12.309174 3 AAGGCGA 70 1.0985896E-4 12.204503 17 CCGTATA 55 0.003035566 12.107851 1 CTATCCC 55 0.0030435934 12.103583 4 CCCTATA 65 7.938563E-4 11.706628 2 CCTAATA 65 7.938563E-4 11.706628 2 CTATACT 130 2.568413E-9 11.704564 4 CCTATAC 90 7.3546E-6 11.625331 3 TAAGACC 140 6.675691E-10 11.547806 4 TAGGACC 240 0.0 11.4911995 4 GGTATCA 1390 0.0 11.429662 1 TTAGGCA 75 2.0528036E-4 11.41195 4 TAGGGCG 75 2.0528036E-4 11.41195 4 TATGGGT 60 0.0058283745 11.094951 4 GACGTGG 335 0.0 10.782188 7 TCAGTAC 115 8.668958E-7 10.752283 3 >>END_MODULE