FastQCFastQC Report
Thu 26 May 2016
SRR1512917_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512917_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857268
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67210.361875615150856No Hit
GTCCTACAGTGGACATTTCTAAATT50990.2745430384844837No Hit
GTCCTAAAGTGTGTATTTCTCATTT50700.27298160523952386No Hit
CTGTAGGACGTGGAATATGGCAAGA49160.2646898562835304No Hit
CTTTAGGACGTGAAATATGGCGAGG39720.2138625120338045No Hit
TATCAACGCAGAGTACTTTTTTTTT38690.20831673188791278No Hit
GGTATCAACGCAGAGTACTTTTTTT36570.19690211644199976No Hit
GTACTGGTTCACTATCGGTCAGTCA28770.15490494640514993No Hit
GTCCTACAGTGTGCATTTCTCATTT26750.1440287562161196No Hit
CTGAAGGACCTGGAATATGGCGAGA20980.11296161889398837No Hit
GTCCTTCAGTGTGCATTTCTCATTT20390.10978490987838051No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTT551.9622908E-413.81667215
ACCGTCT551.9622908E-413.8166728
AAATCGC655.457813E-513.15276317
AATCGCC655.457813E-513.15276318
ATCGCCG655.4619544E-513.151719
GGTATCA16700.012.428311
TAGGACC8000.012.2295854
TAGAAAT6550.011.7464924
CACCGTC752.0763338E-411.3987557
CGTGCGC1251.835906E-811.39844810
GTCCTAG2900.011.1622821
ACACCGT951.3621253E-510.9987996
TGTAGGA26850.010.9311782
CTAGAGT2800.010.8556644
CTAGGGG700.001493669810.8553713
TCGAACT1502.1391315E-910.76491119
GTATCAA36350.010.7648881
CTGTAGG27200.010.745871
AGGACCT15650.010.6822525
TAGAGTG2800.010.5164255
Produced by FastQC (version 0.11.5)