##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512917_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1857268
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.94289138670348	31.0	30.0	34.0	22.0	34.0
2	28.97601584693216	31.0	30.0	34.0	22.0	34.0
3	29.14295998208121	31.0	30.0	34.0	22.0	34.0
4	32.55789363732105	35.0	33.0	37.0	25.0	37.0
5	32.73847231525014	35.0	33.0	37.0	25.0	37.0
6	32.61513416480551	36.0	33.0	37.0	25.0	37.0
7	32.524041226145066	36.0	33.0	37.0	24.0	37.0
8	32.45377942224816	36.0	33.0	37.0	23.0	37.0
9	33.744886575335386	38.0	34.0	39.0	22.0	39.0
10	33.611332882491915	38.0	34.0	39.0	19.0	39.0
11	33.69626731306413	38.0	34.0	39.0	22.0	39.0
12	33.59540518654281	38.0	34.0	39.0	19.0	39.0
13	33.57243058083163	38.0	34.0	39.0	18.0	39.0
14	34.41663884802839	39.0	34.0	41.0	17.0	41.0
15	34.6058474059748	39.0	34.0	41.0	18.0	41.0
16	34.554492404973324	39.0	34.0	41.0	18.0	41.0
17	34.32545814605108	39.0	34.0	41.0	17.0	41.0
18	34.32571928230067	39.0	34.0	41.0	17.0	41.0
19	34.44844470480297	39.0	34.0	41.0	18.0	41.0
20	34.4317960574349	39.0	34.0	41.0	18.0	41.0
21	34.32805227893874	39.0	34.0	41.0	17.0	41.0
22	34.33941628241051	39.0	34.0	41.0	17.0	41.0
23	34.005954444915865	39.0	34.0	41.0	10.0	41.0
24	34.07772653165833	39.0	34.0	41.0	2.0	41.0
25	33.794032955933126	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6130901504114732
1101	2	0.2635640486773845
1101	3	0.6608773008226052
1101	4	0.7881032156871939
1101	5	0.7344452914761703
1101	6	0.7388644973406713
1101	7	0.4182942664310403
1101	8	0.6822054502851529
1101	9	0.7362597362895826
1101	10	0.7699941337300373
1101	11	0.7793291643400195
1101	12	0.7389562586603731
1101	13	1.0886376075684936
1101	14	1.203138891643249
1101	15	1.0590634851933913
1101	16	0.9600371344247023
1101	17	0.18810315454877724
1101	18	0.8247205753048945
1101	19	0.19505417591456364
1101	20	-0.36551574892466476
1101	21	0.793133312657595
1101	22	0.8774564749189437
1101	23	0.3946945414392218
1101	24	0.906589524606602
1101	25	0.6243883605002409
1102	1	0.8657913662504164
1102	2	0.8605238676794258
1102	3	0.7435390730739506
1102	4	0.8620468740850171
1102	5	0.7525444526147922
1102	6	0.6624628467188955
1102	7	0.6076094035421704
1102	8	0.6224816822755628
1102	9	0.9400907683240476
1102	10	1.0416298702700146
1102	11	0.7862740422816046
1102	12	0.8807716127315288
1102	13	0.8161124617738906
1102	14	1.1318422725149375
1102	15	1.0355348675307638
1102	16	1.0622112261995582
1102	17	1.3059658673155212
1102	18	1.1514471584660555
1102	19	0.8942770276169298
1102	20	1.084406957477917
1102	21	1.0760753312943336
1102	22	1.0859324816769274
1102	23	1.1094825626064022
1102	24	0.397229412061435
1102	25	0.7105570188853818
1103	1	0.8461356255210646
1103	2	0.8239017657176788
1103	3	1.0576326712536464
1103	4	1.201735577116807
1103	5	0.9478767200030234
1103	6	0.7040172406676035
1103	7	0.5786821542954996
1103	8	0.8754177870661692
1103	9	0.9206093096191736
1103	10	1.117195456642314
1103	11	0.9047020602386837
1103	12	1.0711533850702537
1103	13	1.2380442002893517
1103	14	1.3012244753314945
1103	15	1.2138594478148832
1103	16	1.2037991974190376
1103	17	0.9367842671679298
1103	18	1.2744383644189483
1103	19	1.2113714252554502
1103	20	1.460703249218895
1103	21	1.2302156670670428
1103	22	1.2022426717021446
1103	23	0.5120562050638142
1103	24	0.7202022919581239
1103	25	1.1105921950738065
1104	1	1.137936096918505
1104	2	0.9410651983211871
1104	3	0.9011295508397623
1104	4	1.227771658292042
1104	5	1.0450481574687416
1104	6	1.0923997544908204
1104	7	1.053305021518554
1104	8	0.8927596391541002
1104	9	1.2089247604856226
1104	10	1.0776133697450874
1104	11	0.8891347971603665
1104	12	1.2484475637565708
1104	13	1.3321960851379302
1104	14	1.3953460009989342
1104	15	1.3493790374915733
1104	16	1.2617880795548544
1104	17	1.5794372563910102
1104	18	1.4987784821200734
1104	19	1.0059256285492992
1104	20	1.1151099851622064
1104	21	1.1834384542414682
1104	22	1.0190932031360518
1104	23	1.3411523968247394
1104	24	1.2011295514787221
1104	25	0.715196947590151
1105	1	0.7255509615966709
1105	2	0.7699348204009162
1105	3	0.8971238473339653
1105	4	0.9990656079304969
1105	5	0.6960104130751077
1105	6	0.964171574487473
1105	7	1.0033795756763695
1105	8	0.9577713041021596
1105	9	0.7751512471956516
1105	10	0.8617086446058124
1105	11	0.8578319937459895
1105	12	1.0363097977082916
1105	13	1.0823801726897244
1105	14	1.0958966855132815
1105	15	1.0926812739313263
1105	16	0.9932077810214395
1105	17	1.3747158283456287
1105	18	1.0923616103239695
1105	19	0.9142492762587366
1105	20	1.0823151736737557
1105	21	1.0477539335390915
1105	22	0.9356933624300225
1105	23	1.0839081224418479
1105	24	1.22944221116434
1105	25	1.4126503135222137
1106	1	0.7847347708592025
1106	2	0.782904518276343
1106	3	0.8020596980340642
1106	4	0.9194821896447536
1106	5	0.7368790163364949
1106	6	0.9005652583691415
1106	7	0.8132523445414108
1106	8	0.8575804769699076
1106	9	0.9480962083523892
1106	10	0.7469038679141207
1106	11	0.5970983678561197
1106	12	0.8905859017604953
1106	13	0.8432210787965886
1106	14	1.1167439181284564
1106	15	0.7699396826187836
1106	16	0.9600480530541802
1106	17	1.1597437087482945
1106	18	0.9952966760815869
1106	19	1.0153730676661468
1106	20	1.2089513748937222
1106	21	1.15637239562826
1106	22	1.0469004267700441
1106	23	1.327148134440158
1106	24	1.0709581962724926
1106	25	1.2415947651423949
1107	1	0.8438597833097212
1107	2	0.8176437755074843
1107	3	0.7960236876659685
1107	4	0.9679307973874671
1107	5	0.7576480992140802
1107	6	0.8769691554387009
1107	7	0.7555603575779699
1107	8	0.8945102143624553
1107	9	0.7027527014993922
1107	10	1.0335081545979747
1107	11	0.8472030680129379
1107	12	0.8713221152746868
1107	13	0.6384974107702064
1107	14	1.0267695372376267
1107	15	0.8687261336772494
1107	16	0.9734029981676713
1107	17	1.1466362683196678
1107	18	1.0552244283406296
1107	19	0.9479835761336517
1107	20	0.9845235774653673
1107	21	0.8790587422182057
1107	22	0.9405064206223841
1107	23	1.2234296574104917
1107	24	0.8854006795193072
1107	25	1.2646532374003243
1108	1	0.8875087263174706
1108	2	0.9046901638339548
1108	3	0.8155485950406742
1108	4	1.0065258762368785
1108	5	0.8512162075960958
1108	6	1.0099539602839513
1108	7	0.8729101883407537
1108	8	0.8449347880886791
1108	9	0.9579510150499928
1108	10	1.1231487092309962
1108	11	0.8672560876304161
1108	12	1.0344464996921445
1108	13	0.9827257965564726
1108	14	1.1706743651596838
1108	15	1.2097188311407763
1108	16	0.8781816736204888
1108	17	1.0596827794884476
1108	18	1.1007367356043432
1108	19	1.0922040649144193
1108	20	1.1072178163804907
1108	21	1.0821736721651831
1108	22	1.0128128288229874
1108	23	1.4149609964574523
1108	24	1.1291787166308467
1108	25	1.361725971014586
1109	1	0.7696078352174887
1109	2	0.5956914292666333
1109	3	0.7436036663516141
1109	4	0.7882970852553441
1109	5	0.6873723198110184
1109	6	0.8889848465267676
1109	7	0.6112489863027193
1109	8	0.6557298297734846
1109	9	0.7976857784105391
1109	10	0.799439860034461
1109	11	0.7835503590735371
1109	12	0.7822113803282278
1109	13	0.8241750278163735
1109	14	0.906297453061427
1109	15	0.8299725582051494
1109	16	0.4960966940370781
1109	17	0.8358667580402539
1109	18	0.9335955640058415
1109	19	0.84408639463593
1109	20	0.795106088615519
1109	21	0.8621043022074844
1109	22	0.8786410599954522
1109	23	1.007379637359044
1109	24	0.776984056980595
1109	25	0.3961152869905433
1110	1	0.44728723383965274
1110	2	0.5286000863372209
1110	3	0.4310488037477711
1110	4	0.35465793114993005
1110	5	0.3585842641629
1110	6	0.25675059913847065
1110	7	0.10617445350947463
1110	8	0.2618548900623665
1110	9	0.40647571330858767
1110	10	0.37298325720500713
1110	11	0.2377572564564474
1110	12	0.17999301728883665
1110	13	0.2235410822686461
1110	14	0.31992192580572265
1110	15	0.3267157868234989
1110	16	0.37586799690855344
1110	17	0.4527480691228547
1110	18	-0.04856595730277746
1110	19	0.2674753786156643
1110	20	0.35613997515212503
1110	21	0.5246516098694869
1110	22	0.4623366473960857
1110	23	0.6687693761633895
1110	24	0.5092331241070909
1110	25	0.6843920036418396
1111	1	0.3156154241602849
1111	2	0.36427042423126466
1111	3	0.35516649493239427
1111	4	0.2234951918039627
1111	5	0.30922926094977043
1111	6	0.08553301731381424
1111	7	0.14275428439661653
1111	8	0.29145086362098027
1111	9	0.3561411893249655
1111	10	0.23917088134238185
1111	11	-0.0098468074696072
1111	12	-0.34427085075977715
1111	13	0.10736174972284829
1111	14	0.08593521505672186
1111	15	0.03205944491342905
1111	16	0.2607292869288429
1111	17	-0.3230965992056909
1111	18	0.1353839476833727
1111	19	0.10555383955380648
1111	20	0.2759883314025231
1111	21	0.26267281987201585
1111	22	-0.09528267973625049
1111	23	0.31130472895002015
1111	24	0.2681886551328887
1111	25	0.3655672861068666
1112	1	0.2813703850490654
1112	2	0.2867075705126929
1112	3	0.151257321436848
1112	4	0.30506127458659194
1112	5	0.1245667174064451
1112	6	0.12278426989353619
1112	7	0.01524013011847103
1112	8	0.11580773542311107
1112	9	0.12234867086091583
1112	10	0.20263523045149867
1112	11	0.052657745302589376
1112	12	0.020238870197630376
1112	13	0.04140151831249028
1112	14	-0.016796470400024077
1112	15	-0.2995745558203282
1112	16	0.028200916930885
1112	17	0.36957074188013905
1112	18	0.0026066226919354563
1112	19	0.18965906842789337
1112	20	-0.1493571645025824
1112	21	-0.0817163602835862
1112	22	-0.06985911416670376
1112	23	0.1256853559827178
1112	24	-0.010154939512403871
1112	25	-0.10416710211900693
1113	1	0.001389613322231753
1113	2	0.13989231959102355
1113	3	0.15822569097170813
1113	4	0.20380294596487403
1113	5	0.06926431544222567
1113	6	-0.10432328350549369
1113	7	-0.0617326639534852
1113	8	0.11487141846439641
1113	9	-0.21413151590275703
1113	10	-0.09349222717322192
1113	11	-0.014755859585143583
1113	12	-0.3072924083609223
1113	13	-0.08150111069394228
1113	14	0.19955355404438535
1113	15	-0.21002024446160306
1113	16	-0.32149550144945493
1113	17	-0.18350004385882812
1113	18	-0.20914362998870928
1113	19	-0.6549382975905544
1113	20	-0.3790671461565651
1113	21	-0.2346115354596563
1113	22	-0.5911270746142989
1113	23	-4.094321379219686
1113	24	-1.7645986584497138
1113	25	-0.9778695640499677
1114	1	-0.5007462741728794
1114	2	-0.8034888926849924
1114	3	-0.7287307497949378
1114	4	-0.6429362673642025
1114	5	-0.592882849982054
1114	6	-1.2642203441126938
1114	7	-1.083182535573922
1114	8	-1.0227041120125904
1114	9	-1.2082357096822847
1114	10	-0.7979056952932098
1114	11	-1.012990500626266
1114	12	-1.8435007728171975
1114	13	-1.0330551676071735
1114	14	-2.7844811274737538
1114	15	-1.6625112016675914
1114	16	-1.4402239950879974
1114	17	-1.483334894333737
1114	18	-1.4628089864369258
1114	19	-1.472773574052468
1114	20	-1.1446295509533613
1114	21	-1.1809458961202353
1114	22	-1.2227203402478395
1114	23	-5.3092938203126
1114	24	-2.459340194315544
1114	25	-2.357179632489597
1115	1	-1.7359614154803076
1115	2	-1.5844225888903694
1115	3	-1.375171846121443
1115	4	-1.2908631814896445
1115	5	-1.2955121369418485
1115	6	-1.5349806805142236
1115	7	-1.997298620322276
1115	8	-2.278240903425818
1115	9	-3.607690394038663
1115	10	-4.280712515109347
1115	11	-2.919380620699588
1115	12	-3.2190309957810292
1115	13	-2.5625842220203445
1115	14	-3.297117895700694
1115	15	-2.827630696228052
1115	16	-2.9888200250145047
1115	17	-2.9986310689076774
1115	18	-5.649245714192812
1115	19	-3.5720172547687277
1115	20	-3.7912012725792117
1115	21	-3.4235922855182146
1115	22	-2.9096779498792316
1115	23	-3.4006629185890063
1115	24	-3.0669294402429195
1115	25	-3.2774141150976597
1116	1	-1.9265396178507679
1116	2	-1.694625044311433
1116	3	-2.071197954470211
1116	4	-1.8688267918067183
1116	5	-2.045925389741239
1116	6	-4.368375534866871
1116	7	-2.7719588448593484
1116	8	-3.1726990314144423
1116	9	-4.28991199128545
1116	10	-3.5474097958070523
1116	11	-3.1820640210835087
1116	12	-2.8603737066754498
1116	13	-4.298662344926157
1116	14	-7.416146420214254
1116	15	-5.256694031385045
1116	16	-4.194886876390708
1116	17	-5.2738263065948665
1116	18	-3.738079868904993
1116	19	-3.742368957253081
1116	20	-4.262357069433875
1116	21	-5.1571681874419895
1116	22	-4.663195084581702
1116	23	-3.8174583033407288
1116	24	-4.770198183829901
1116	25	-4.6848985865332295
1201	1	0.5286912241022534
1201	2	0.6405575176422147
1201	3	1.0455545435914324
1201	4	0.8526652715143541
1201	5	0.3710044906385477
1201	6	0.9701219168653097
1201	7	0.9659118440462819
1201	8	0.9510370618605464
1201	9	1.4685204014548034
1201	10	1.263679061295278
1201	11	1.3338557598406098
1201	12	1.1358914157699047
1201	13	1.307550062961866
1201	14	1.3796615503458227
1201	15	1.353703378361189
1201	16	1.3860189275967016
1201	17	1.5345307704748805
1201	18	1.4041922405980642
1201	19	1.5929969886760915
1201	20	1.5367723311723935
1201	21	1.6779352500970433
1201	22	1.446890059443966
1201	23	1.8887851900547545
1201	24	1.6626487844226148
1201	25	1.7054548520958477
1202	1	0.8347082450977759
1202	2	0.9329513128510563
1202	3	0.9874449959689748
1202	4	1.1021900069339807
1202	5	0.8476120803806424
1202	6	1.0098722313957182
1202	7	1.0960899488120646
1202	8	1.1009667594957122
1202	9	1.406382830331495
1202	10	1.284598990687769
1202	11	1.0822514004786754
1202	12	1.256754096170468
1202	13	1.2254786917147769
1202	14	1.5780940405488835
1202	15	1.2453995621426301
1202	16	1.1416822643452207
1202	17	1.4156519013804285
1202	18	1.3462018622963754
1202	19	1.5086120499202025
1202	20	1.272061481276637
1202	21	1.3849900613291979
1202	22	1.4687501581774498
1202	23	1.514462298457019
1202	24	1.4690307850080373
1202	25	1.8569876219638175
1203	1	0.9782327203339953
1203	2	1.0200183407517542
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2211	16	-0.49850388818401115
2211	17	-0.07880123703694863
2211	18	-0.37126505879074756
2211	19	-0.31236390330272457
2211	20	-0.4332312064284167
2211	21	-0.054190738334668254
2211	22	-0.15981520806730742
2211	23	0.1305759063883869
2211	24	-0.29280535741760616
2211	25	0.080699347164078
2212	1	-0.15295804521391787
2212	2	-0.03854602754423553
2212	3	-0.47671354342213235
2212	4	-0.15665205679444227
2212	5	-0.35281702235023005
2212	6	-0.44698440058451894
2212	7	-0.30120397264789034
2212	8	-0.23995459812078934
2212	9	-0.21933614112834476
2212	10	-0.558381876173776
2212	11	-0.5209345366996345
2212	12	-0.304351613288496
2212	13	-0.23250373919320566
2212	14	-0.11717020104342879
2212	15	-0.47360842840486583
2212	16	-0.47895010877892474
2212	17	-0.27969653018219276
2212	18	0.033115813614735146
2212	19	-0.38881275307405616
2212	20	-0.2142155535622834
2212	21	-0.1049422213874962
2212	22	-0.09790712985555672
2212	23	0.014339037566578838
2212	24	-0.09765190517117617
2212	25	0.071322343383315
2213	1	-0.30193954436287385
2213	2	-0.1877124023020471
2213	3	-0.5075554939998277
2213	4	-0.3379566473103992
2213	5	-0.24833002724575692
2213	6	-0.011487239457473208
2213	7	-0.22689821734440585
2213	8	-0.041012304941773436
2213	9	-0.5614953268592728
2213	10	-0.19021978179655719
2213	11	-0.4176211101263405
2213	12	-0.3235319237276286
2213	13	-0.39681947160913467
2213	14	-0.4284758073072723
2213	15	-0.2348388503389458
2213	16	-0.32070726719097564
2213	17	-0.24499948410996097
2213	18	-0.28833991449138807
2213	19	-0.34647979851025923
2213	20	-0.19771012500685714
2213	21	-0.3778193320309242
2213	22	-0.5257764515163288
2213	23	-0.33368015911923976
2213	24	-0.22710470184787823
2213	25	1.3407973313661614E-4
2214	1	-2.7120610618495355
2214	2	-1.5065650470944014
2214	3	-1.797780920269922
2214	4	-3.481284517877711
2214	5	-1.7755915542632046
2214	6	-1.600655271815647
2214	7	-1.4577523847374998
2214	8	-1.3318857082450002
2214	9	-1.1044012859233945
2214	10	-1.1060321648410607
2214	11	-1.1077791235391743
2214	12	-1.0812364578234224
2214	13	-1.3888200062628329
2214	14	-1.379539012672403
2214	15	-1.670160281816102
2214	16	-1.3906030678348884
2214	17	-3.4262151238928453
2214	18	-3.1951706965967617
2214	19	-2.0272014647701226
2214	20	-1.9171288053420596
2214	21	-1.9558671486679486
2214	22	-3.0699769117834244
2214	23	-1.9491086956790937
2214	24	-3.1025521837452033
2214	25	-2.8616550342527063
2215	1	-1.8383348223825564
2215	2	-3.34062891955697
2215	3	-1.8664069303447341
2215	4	-3.577018284635443
2215	5	-2.291686469595593
2215	6	-2.078436507766817
2215	7	-2.1141136946935823
2215	8	-1.5490748472750973
2215	9	-3.9583770326006658
2215	10	-2.3584730588941554
2215	11	-1.895538054271963
2215	12	-1.8920037688630664
2215	13	-1.9409087429920895
2215	14	-1.6252667276267019
2215	15	-1.7682686609702358
2215	16	-1.8792069455999822
2215	17	-2.3167754632549276
2215	18	-1.8488317494952895
2215	19	-1.9149203383277822
2215	20	-1.708876162721225
2215	21	-2.3448050283465705
2215	22	-2.272293023413262
2215	23	-2.1856088512968945
2215	24	-2.0784357470470667
2215	25	-2.916928172314517
2216	1	-3.347509845679614
2216	2	-3.41390891989532
2216	3	-4.645046805041098
2216	4	-5.167946952635784
2216	5	-4.965649591866736
2216	6	-3.9278976795701155
2216	7	-5.7041199965304195
2216	8	-3.4324817020381992
2216	9	-4.355207363750207
2216	10	-3.1339778754994256
2216	11	-3.380427391479447
2216	12	-3.764949151534001
2216	13	-3.333145218362059
2216	14	-4.231087185898531
2216	15	-3.7930921883916824
2216	16	-4.04982107158315
2216	17	-3.6295784836252274
2216	18	-3.3844579222784006
2216	19	-4.000777226009873
2216	20	-4.692487509974995
2216	21	-4.247833575526464
2216	22	-3.9116355176718223
2216	23	-3.9952944361623324
2216	24	-4.216841424621407
2216	25	-4.206973737273483
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92417.0
3	6.0
4	876.0
5	5241.0
6	6426.0
7	5620.0
8	5024.0
9	4631.0
10	3975.0
11	3987.0
12	3683.0
13	3604.0
14	3437.0
15	3551.0
16	3654.0
17	4039.0
18	4498.0
19	5504.0
20	7122.0
21	9337.0
22	11607.0
23	13962.0
24	16345.0
25	18289.0
26	20610.0
27	22535.0
28	25150.0
29	29727.0
30	35827.0
31	44479.0
32	55778.0
33	70603.0
34	91172.0
35	120444.0
36	163347.0
37	249533.0
38	691228.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.657188358946875	18.572376268432244	12.529766020709804	24.240669351911077
2	14.761201914197141	20.119963103187764	42.22385681538293	22.894978167232164
3	19.305000177696982	25.926797458825458	28.97656230648529	25.79164005699227
4	12.147958521556374	16.621371080433008	38.23493143197773	32.99573896603289
5	12.779572156460247	39.333097832349864	34.78326191119818	13.104068099991705
6	31.284265638926524	36.40022589590466	18.90654958016341	13.408958885005404
7	28.512168231007244	31.870515925234976	21.814387191267713	17.802928652490067
8	25.458549813475372	36.85555215978862	20.769319084048465	16.91657894268754
9	28.136170295320177	14.608812990832847	20.067448173063088	37.187568540783886
10	16.32367532224659	28.605666754154836	33.592115070474684	21.478542853123894
11	34.27482981172585	22.304525994938544	24.911618502343188	18.509025690992416
12	24.538560626067834	24.736578293512416	31.186219608815396	19.538641471604354
13	29.475417751642553	22.314199675800584	25.79373889875666	22.416643673800206
14	21.23142753978562	23.092257365661226	27.510522373179132	28.16579272137403
15	23.206167588902137	31.83312891105799	23.338305572860193	21.622397927179684
16	21.6291736702754	28.11163666993427	28.49571915362814	21.76347050616219
17	21.361029665401862	29.042288289065194	27.751433684028974	21.845248361503966
18	21.176592335317334	26.861099927183197	31.017856016764572	20.944451720734893
19	23.557104637400588	26.707630982690862	27.899749708424327	21.835514671484223
20	24.363833914234327	26.774083497758767	28.502827614418276	20.35925497358863
21	23.44280122260878	26.490970542715864	27.131482176036698	22.934746058638662
22	23.80146464747185	28.480370306689874	27.034425737349803	20.683739308488477
23	22.03433495362523	28.086365025409243	28.395727775870462	21.483572245095065
24	23.308235017674452	26.565303316674775	28.92078320541654	21.205678460234235
25	22.615329644538026	27.451093461756727	28.594972107684658	21.33860478602059
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	265.0
2	628.5
3	992.0
4	992.0
5	992.0
6	1780.0
7	2568.0
8	2568.0
9	2568.0
10	2904.0
11	3240.0
12	3240.0
13	3240.0
14	4687.5
15	6135.0
16	6135.0
17	6135.0
18	11913.5
19	17692.0
20	17692.0
21	17692.0
22	30382.5
23	43073.0
24	43073.0
25	43073.0
26	66087.0
27	89101.0
28	89101.0
29	89101.0
30	116595.0
31	144089.0
32	144089.0
33	144089.0
34	163914.5
35	183740.0
36	183740.0
37	183740.0
38	194427.0
39	205114.0
40	205114.0
41	205114.0
42	218117.5
43	231121.0
44	231121.0
45	231121.0
46	244385.0
47	257649.0
48	257649.0
49	257649.0
50	245259.0
51	232869.0
52	232869.0
53	232869.0
54	202126.0
55	171383.0
56	171383.0
57	171383.0
58	145458.0
59	119533.0
60	119533.0
61	119533.0
62	98332.0
63	77131.0
64	77131.0
65	77131.0
66	58456.5
67	39782.0
68	39782.0
69	39782.0
70	29574.5
71	19367.0
72	19367.0
73	19367.0
74	13673.0
75	7979.0
76	7979.0
77	7979.0
78	5504.0
79	3029.0
80	3029.0
81	3029.0
82	2023.0
83	1017.0
84	1017.0
85	1017.0
86	646.5
87	276.0
88	276.0
89	276.0
90	182.0
91	88.0
92	88.0
93	88.0
94	57.0
95	26.0
96	26.0
97	26.0
98	17.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3318853283424902
2	0.010606977560588994
3	0.009368599469758807
4	0.011145402817471685
5	0.012599151011054946
6	0.08324054471406388
7	0.08259443440580466
8	0.10860037431323857
9	0.08727873414068406
10	0.09578585319943056
11	0.09024007305353884
12	0.1011162632425692
13	0.08722489161499579
14	0.09142460861868078
15	0.08787100192325502
16	0.09029391557922711
17	0.10273153901321727
18	0.10375454700129437
19	0.09863950706090882
20	0.09783186917558478
21	0.08420971017645272
22	0.10450834236093014
23	0.09912408979210324
24	0.09503205783979479
25	0.0936321521718998
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1857268.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.325473119489175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.93778232022707	42.77231516831571
2	13.846846364343904	15.598603453690979
3	4.697376778444993	7.937459083992484
4	2.095369744433691	4.720907688619631
5	1.086089977692298	3.058726592192707
6	0.6101710058002096	2.062090235129483
7	0.40051497211165915	1.5791436706939748
8	0.260047881629993	1.1717855973224218
9	0.1872503556238568	0.9492268385075702
>10	0.7778230931526673	7.5684455734646106
>50	0.05124508721099213	2.0176986325192603
>100	0.04109185288139269	4.656336265243691
>500	0.005593710964822171	2.285984328763111
>1k	0.002507525604920283	2.7037759135860067
>5k	2.893298774908019E-4	0.9175009579584845
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6721	0.361875615150856	No Hit
GTCCTACAGTGGACATTTCTAAATT	5099	0.2745430384844837	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5070	0.27298160523952386	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4916	0.2646898562835304	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3972	0.2138625120338045	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3869	0.20831673188791278	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3657	0.19690211644199976	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2877	0.15490494640514993	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2675	0.1440287562161196	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2098	0.11296161889398837	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2039	0.10978490987838051	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.23055154129614E-4
13	0.0	0.0	0.0	0.0	3.7689767981788304E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTT	55	1.9622908E-4	13.816672	15
ACCGTCT	55	1.9622908E-4	13.816672	8
AAATCGC	65	5.457813E-5	13.152763	17
AATCGCC	65	5.457813E-5	13.152763	18
ATCGCCG	65	5.4619544E-5	13.1517	19
GGTATCA	1670	0.0	12.42831	1
TAGGACC	800	0.0	12.229585	4
TAGAAAT	655	0.0	11.746492	4
CACCGTC	75	2.0763338E-4	11.398755	7
CGTGCGC	125	1.835906E-8	11.398448	10
GTCCTAG	290	0.0	11.162282	1
ACACCGT	95	1.3621253E-5	10.998799	6
TGTAGGA	2685	0.0	10.931178	2
CTAGAGT	280	0.0	10.855664	4
CTAGGGG	70	0.0014936698	10.855371	3
TCGAACT	150	2.1391315E-9	10.764911	19
GTATCAA	3635	0.0	10.764888	1
CTGTAGG	2720	0.0	10.74587	1
AGGACCT	1565	0.0	10.682252	5
TAGAGTG	280	0.0	10.516425	5
>>END_MODULE