FastQCFastQC Report
Thu 26 May 2016
SRR1512917_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512917_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857268
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT79810.4297171975180749No Hit
TATCAACGCAGAGTACTTTTTTTTT58260.31368655465985523No Hit
GTCCTACAGTGGACATTTCTAAATT46870.25235991790091683No Hit
CTGTAGGACGTGGAATATGGCAAGA46350.24956010656512684No Hit
GGTATCAACGCAGAGTACTTTTTTT45660.2458449722926363No Hit
GTCCTAAAGTGTGTATTTCTCATTT45320.24401432641923512No Hit
CTTTAGGACGTGAAATATGGCGAGG43000.23152286045955672No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26660.14354417348492518No Hit
GTACTGGTTCACTATCGGTCAGTCA25880.1393444564812402No Hit
GTCCTACAGTGTGCATTTCTCATTT25860.13923677142986365No Hit
CTGAAGGACCTGGAATATGGCGAGA22460.1209303126958522No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAG250.005993773719.0212021
GGTATCA17150.012.8656531
AATCGCC550.003089097812.08028818
GGCGAGG10800.011.77668219
ATCGCCG658.08409E-411.68203619
TAGGACC7450.011.4880774
ATAGGAC2500.011.4139533
GTACTAA1408.20728E-910.8692581
CCGTCTC700.001494199910.854919
GCGCGCC2400.010.68012411
TAGGACA6550.010.5984584
GTGTCGA2450.010.4618813
CCCGTAC2550.010.42305116
GTATCAA36800.010.3892981
GATATAC2200.010.3752011
GTAGGAC24800.010.2786943
GACGTGG12550.010.226947
TGTAGGA25700.010.2148222
AGCACCG750.002625947410.1435475
GTCCTAC24100.010.1420111
Produced by FastQC (version 0.11.5)