##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512917_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1857268
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.218233986694436	31.0	30.0	34.0	26.0	34.0
2	30.23528752985568	31.0	30.0	34.0	26.0	34.0
3	30.432363557655652	31.0	30.0	34.0	26.0	34.0
4	34.02864530051667	37.0	35.0	37.0	30.0	37.0
5	33.599250081302216	37.0	35.0	37.0	28.0	37.0
6	33.46565116073717	37.0	35.0	37.0	28.0	37.0
7	33.301395921320996	37.0	35.0	37.0	27.0	37.0
8	33.2461825649287	37.0	35.0	37.0	27.0	37.0
9	34.562848764960144	39.0	35.0	39.0	27.0	39.0
10	34.48563858312317	39.0	35.0	39.0	27.0	39.0
11	34.46793031484955	39.0	35.0	39.0	27.0	39.0
12	34.33207162348137	39.0	35.0	39.0	26.0	39.0
13	34.318300320686085	39.0	35.0	39.0	26.0	39.0
14	35.39947438926423	40.0	36.0	41.0	25.0	41.0
15	35.402439497153885	40.0	36.0	41.0	26.0	41.0
16	35.40541806567496	40.0	36.0	41.0	26.0	41.0
17	35.402760398606986	40.0	36.0	41.0	26.0	41.0
18	35.33218038538326	40.0	36.0	41.0	25.0	41.0
19	35.3449932912213	40.0	36.0	41.0	25.0	41.0
20	35.32162025082002	40.0	36.0	41.0	25.0	41.0
21	35.288776310150176	40.0	36.0	41.0	25.0	41.0
22	35.251487668984765	40.0	36.0	41.0	25.0	41.0
23	35.08904584583377	40.0	35.0	41.0	24.0	41.0
24	35.04968103687782	40.0	35.0	41.0	24.0	41.0
25	34.98960893096742	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4459199626226784
1101	2	0.41471198367226947
1101	3	0.312483414157537
1101	4	0.31027529311701585
1101	5	0.4448723572071458
1101	6	0.4233190307517134
1101	7	0.5377018107999163
1101	8	0.3216399028794541
1101	9	0.5631661624396571
1101	10	0.26826511771558614
1101	11	0.5903815338203415
1101	12	0.633484365048723
1101	13	0.7148371230879675
1101	14	0.7325112728997141
1101	15	0.3963108704157037
1101	16	0.6191632186412761
1101	17	0.6806883521899962
1101	18	0.7113768780487959
1101	19	0.5889108775743708
1101	20	0.606454054221679
1101	21	0.656751978622701
1101	22	0.59720164914701
1101	23	0.8269164116470407
1101	24	0.7306144250376221
1101	25	0.7834990445419407
1102	1	0.8117799186013634
1102	2	0.8712877504293246
1102	3	0.5715328044110315
1102	4	0.6013958168255442
1102	5	0.8779156999852162
1102	6	0.7439213777771272
1102	7	0.7596377134475389
1102	8	0.7737388072186064
1102	9	0.9282280607561972
1102	10	0.8143859992178548
1102	11	0.9086896871022248
1102	12	0.8492262490941158
1102	13	0.9788356127416407
1102	14	0.8560077145014304
1102	15	1.021904719205473
1102	16	1.1138589899253049
1102	17	1.1922529562899626
1102	18	0.5776977789379032
1102	19	0.9512645818053187
1102	20	0.9573103992749168
1102	21	0.8906839947675707
1102	22	0.6107539116468246
1102	23	0.9909228438141682
1102	24	0.9686605616519017
1102	25	1.091663364741457
1103	1	0.8442857915489057
1103	2	0.9654841713136477
1103	3	0.7937310702003444
1103	4	0.6123361034007857
1103	5	0.874470647405424
1103	6	0.770452710413494
1103	7	0.9148289071010609
1103	8	0.9222095039777187
1103	9	0.970807852896904
1103	10	0.9789571818019525
1103	11	1.1027076441774355
1103	12	1.2137973086845477
1103	13	1.2341306130491247
1103	14	1.3115213975467555
1103	15	1.4020694077240137
1103	16	1.351981614610132
1103	17	1.301532582389214
1103	18	1.305031891231124
1103	19	1.313736262513764
1103	20	1.1886853454651884
1103	21	1.439184701981148
1103	22	1.3334024572467271
1103	23	1.3455870096096305
1103	24	1.4160872881754827
1103	25	1.3234524306045614
1104	1	0.6220584831097042
1104	2	0.8516545562882243
1104	3	0.7417898318185117
1104	4	0.5522166234203567
1104	5	0.7644020131238634
1104	6	0.513876953286946
1104	7	0.5007519714809092
1104	8	0.683537006608951
1104	9	0.8072987584840021
1104	10	0.860689078213106
1104	11	0.7894752511683478
1104	12	0.8697548532671817
1104	13	0.785403415788565
1104	14	0.8769084919300099
1104	15	0.9674416782883597
1104	16	0.8716138653105645
1104	17	0.8679592415451438
1104	18	0.44519228495175156
1104	19	0.9727307925245796
1104	20	0.9601780743820072
1104	21	0.9633033518277259
1104	22	0.9543533755823077
1104	23	0.9457599146334204
1104	24	1.170348928579287
1104	25	0.9385583764383298
1105	1	0.6181620355225448
1105	2	0.6243059384565264
1105	3	0.6298448302074213
1105	4	0.5180992950501278
1105	5	0.5373282115023201
1105	6	0.6611712738174447
1105	7	0.7456418871827424
1105	8	0.7320459407949187
1105	9	0.9618957677390796
1105	10	0.5820187354605224
1105	11	0.9402762820355122
1105	12	0.9957826537536221
1105	13	1.0930737477889778
1105	14	0.9920135909210757
1105	15	1.1346352590811435
1105	16	0.9894742945163699
1105	17	0.8610815525633342
1105	18	0.888682060042953
1105	19	1.1537842669957499
1105	20	1.1122966506976155
1105	21	0.9640134574914896
1105	22	0.9641038593432398
1105	23	1.2318180385509052
1105	24	1.087457214605962
1105	25	0.9057411356925087
1106	1	0.12691827174112902
1106	2	0.41049023910871085
1106	3	-1.2195753762616306
1106	4	-0.2692400025651196
1106	5	0.02711203919641747
1106	6	0.04493129064069734
1106	7	0.1223996904397211
1106	8	0.295335041943261
1106	9	0.42401161827549316
1106	10	0.2849002144828816
1106	11	0.3312139324813401
1106	12	0.5162977268909756
1106	13	0.4806713007325598
1106	14	0.5096707048448366
1106	15	0.49052697117733857
1106	16	0.4639492530364606
1106	17	0.4604061319732864
1106	18	0.6110501098238217
1106	19	0.6371113556597408
1106	20	0.6455088623531182
1106	21	0.6149524529430224
1106	22	0.5153928765176801
1106	23	0.5382459204480199
1106	24	0.6400745138700898
1106	25	0.7018841717119741
1107	1	0.5937951494527347
1107	2	0.7228829619845385
1107	3	0.5901353316457794
1107	4	0.4633870679847192
1107	5	0.48702751466716165
1107	6	0.5191806113636801
1107	7	0.37568349967232706
1107	8	0.3721876208795365
1107	9	0.5095726095914088
1107	10	0.453559542873073
1107	11	0.43903992174725914
1107	12	0.7137339628740094
1107	13	0.5618484362567244
1107	14	0.6600978444226513
1107	15	0.5302456242515134
1107	16	0.6321814418488358
1107	17	0.5943549261842023
1107	18	0.7074246352564586
1107	19	0.6655830808264582
1107	20	0.7231126417508165
1107	21	0.7695158278089664
1107	22	0.7379132548280722
1107	23	0.8493180778503415
1107	24	0.8710950624340512
1107	25	0.935460492798633
1108	1	0.5592853451010456
1108	2	0.5645397756744366
1108	3	0.5020411642424243
1108	4	0.35689880052191825
1108	5	0.6940746049496767
1108	6	0.6655807833429108
1108	7	0.5690177689470559
1108	8	0.5287370901349533
1108	9	0.7580267707649
1108	10	0.6642105193597345
1108	11	0.6993637209088561
1108	12	0.8051725121063527
1108	13	0.7449824067614088
1108	14	0.8201070795318657
1108	15	0.8130990316997071
1108	16	0.7166588904141022
1108	17	0.7503648764111333
1108	18	0.8753681672799161
1108	19	0.870621150973804
1108	20	0.8824495345862573
1108	21	0.8480353147668396
1108	22	0.8591409540135828
1108	23	0.9648959083235695
1108	24	0.9634820200907441
1108	25	1.0142682204572324
1109	1	0.15092526672600215
1109	2	0.06608120100221626
1109	3	0.2031681074528784
1109	4	0.07324639766817143
1109	5	-0.16123941994296587
1109	6	0.018827883148304636
1109	7	0.11906286640110864
1109	8	0.17590400701057973
1109	9	0.3194008096804737
1109	10	-0.028670554681092142
1109	11	0.09291882430165543
1109	12	0.36634098331762743
1109	13	0.2588458151670352
1109	14	0.3918155776564305
1109	15	0.3402046572621771
1109	16	0.3665942667985931
1109	17	0.4574580482277213
1109	18	0.5560921556790248
1109	19	0.41978446825364557
1109	20	0.5465253093908871
1109	21	0.49134930552748557
1109	22	0.5296760982783368
1109	23	0.4758866881410526
1109	24	0.4670813388249684
1109	25	0.436476358921432
1110	1	0.2412026926713935
1110	2	0.04882100456480032
1110	3	0.1264800912868651
1110	4	0.12324772564170416
1110	5	0.2714350004523851
1110	6	0.2658806188742062
1110	7	0.169504545932746
1110	8	0.14260211354351782
1110	9	-0.021171727693051423
1110	10	0.23908874777347222
1110	11	0.24964235427402315
1110	12	0.3035156716511622
1110	13	0.3111453185049342
1110	14	0.3173671497873798
1110	15	0.37532821794532367
1110	16	0.2061718283758367
1110	17	0.2654252644671189
1110	18	0.29363994877672894
1110	19	0.37279406916746893
1110	20	0.3528116905648204
1110	21	0.4937010462106386
1110	22	0.5613626626010202
1110	23	0.5489339365464261
1110	24	0.549933272454858
1110	25	0.4028559749964771
1111	1	0.05550443047768994
1111	2	-0.07954474165729408
1111	3	-0.2343540584065238
1111	4	-0.0038220837718938583
1111	5	0.06618293940757525
1111	6	0.12598366165458685
1111	7	-0.07057887749513014
1111	8	-0.05012619403913732
1111	9	-0.10540517172191954
1111	10	-0.1937638290639896
1111	11	-0.09879670074720082
1111	12	0.1072002768701239
1111	13	0.016394151950343883
1111	14	-0.13250767500405658
1111	15	-0.1506045415876116
1111	16	-0.1489795204379547
1111	17	0.02607428351062424
1111	18	-0.17362473641101417
1111	19	-0.4264327760900599
1111	20	-0.1331183132660101
1111	21	-0.0025370197405720774
1111	22	-0.2168122211998309
1111	23	-0.11585448986085822
1111	24	-0.034786426118515124
1111	25	-0.18593684272673983
1112	1	0.0615753100278873
1112	2	-0.28084247823483466
1112	3	-0.07865622387657112
1112	4	0.028108873949818758
1112	5	-0.04998277286519226
1112	6	-0.03427236964766678
1112	7	0.023678507516514458
1112	8	-0.07415043947457178
1112	9	-0.02111794266583189
1112	10	-0.2276840576314143
1112	11	-0.24592845980109956
1112	12	0.1657306054734491
1112	13	-0.08735901506316424
1112	14	-0.22264691842797646
1112	15	-0.5637800484370885
1112	16	-0.5933578735754352
1112	17	-0.3953716038720003
1112	18	-0.15815959199209573
1112	19	-0.16983178226278994
1112	20	-0.25856337788382433
1112	21	-0.2798199430088957
1112	22	-0.2090281708367101
1112	23	0.07777754075414123
1112	24	0.014922982471667012
1112	25	-0.1502982401781594
1113	1	-0.6109755561178147
1113	2	-0.5782578277301482
1113	3	-0.4972253935870903
1113	4	-2.0035484568182724
1113	5	-0.7524166220554775
1113	6	-0.39962636467741675
1113	7	-0.6200355873428194
1113	8	-0.5792172088571306
1113	9	-0.48623574126735747
1113	10	-0.5497224757035184
1113	11	-0.6094660950109514
1113	12	-0.43787995831340965
1113	13	-0.5648601001913889
1113	14	-0.6600322005292512
1113	15	-0.6877636044603292
1113	16	-0.9680481725622556
1113	17	-1.0068157652630134
1113	18	-0.7976040417187633
1113	19	-0.7088958456241059
1113	20	-0.41326739492569686
1113	21	-0.46542042964164665
1113	22	-0.437114384965291
1113	23	-0.3014117009651045
1113	24	-0.17108811712419936
1113	25	-0.4052625774362326
1114	1	-0.7891988980129305
1114	2	-1.8948564570987116
1114	3	-1.2484087923056144
1114	4	-0.8030435431749368
1114	5	-0.7268009417734547
1114	6	-0.4367624354624553
1114	7	-0.3443381927589826
1114	8	-0.4360155617702688
1114	9	-0.809125399436958
1114	10	-0.799226974086146
1114	11	-0.7434549186868722
1114	12	-0.8712589039236818
1114	13	-0.7642494795363532
1114	14	-0.8078536966535808
1114	15	-0.8251323886187052
1114	16	-1.2372131378544537
1114	17	-1.2935652475426522
1114	18	-0.9999846418889504
1114	19	-1.4918568843157232
1114	20	-0.9311677481507203
1114	21	-1.0721134939863077
1114	22	-0.7536396983645375
1114	23	-0.4625362736373262
1114	24	-0.6447056248833718
1114	25	-1.2217159303865017
1115	1	-1.1200543090010235
1115	2	-1.173930751947001
1115	3	-0.7312945390703014
1115	4	-0.5897338687342
1115	5	-0.9211322835807607
1115	6	-0.8552749791496623
1115	7	-0.7382509578494734
1115	8	-1.0010839337388262
1115	9	-2.050311760236511
1115	10	-1.7330033059094347
1115	11	-2.0498898759841566
1115	12	-2.590178689074456
1115	13	-2.740768232543342
1115	14	-2.0256195050436574
1115	15	-2.4018177758459487
1115	16	-2.022055037460575
1115	17	-2.1660462873824073
1115	18	-2.5233220091330395
1115	19	-2.7875517791215785
1115	20	-2.8219183374339565
1115	21	-2.246566400087346
1115	22	-2.123383424481787
1115	23	-2.04386863489772
1115	24	-2.5659846300871507
1115	25	-2.673438247149136
1116	1	-1.3809327266218148
1116	2	-1.5518525426188035
1116	3	-1.6022413841772192
1116	4	-1.2933138793831986
1116	5	-1.721687364301463
1116	6	-2.5180141878868163
1116	7	-1.9311449871613071
1116	8	-2.310415280822106
1116	9	-3.2661245664225937
1116	10	-2.0211769152963655
1116	11	-3.075701971748945
1116	12	-5.348965392038522
1116	13	-4.0042737441675165
1116	14	-3.4732977090986275
1116	15	-4.610814931963592
1116	16	-4.031945639905157
1116	17	-3.8089720659345723
1116	18	-3.800059666661639
1116	19	-4.094890650811433
1116	20	-5.796608297130362
1116	21	-4.737618753742392
1116	22	-3.9529742291401
1116	23	-4.6247885499108286
1116	24	-4.461256812992072
1116	25	-3.705731655538113
1201	1	0.305791530307598
1201	2	0.5988676690909429
1201	3	0.7142215210917477
1201	4	0.5642203678948334
1201	5	-0.092947453305527
1201	6	0.44799834942156025
1201	7	0.7369310430655034
1201	8	0.6339142173495702
1201	9	0.6102861757233811
1201	10	0.630787357914464
1201	11	0.43313970068213337
1201	12	0.8513487716420656
1201	13	0.8232486254714857
1201	14	0.8618999852390132
1201	15	0.6734715235524575
1201	16	0.8006780102669566
1201	17	0.5604149417083093
1201	18	0.829114851532232
1201	19	0.8772381909225544
1201	20	0.9963976175799303
1201	21	0.6436592299469481
1201	22	0.6314324134122131
1201	23	0.7053840387758399
1201	24	0.5209546849010636
1201	25	0.6829036163049054
1202	1	0.7069875432461963
1202	2	0.7376984209036905
1202	3	0.7099797713696745
1202	4	0.6328200806578437
1202	5	0.6124259277842725
1202	6	0.6121407151950322
1202	7	0.6850726734801214
1202	8	0.6855401502513416
1202	9	0.9329809996686009
1202	10	0.8482791538790053
1202	11	0.8708029070852845
1202	12	0.9251175949636874
1202	13	0.8263202806404237
1202	14	0.930602668331872
1202	15	0.9239720912077942
1202	16	0.9641685236230231
1202	17	0.8315453638025261
1202	18	0.9217139344182002
1202	19	1.0003796549521908
1202	20	0.7890162522712103
1202	21	0.9394878390978221
1202	22	0.7959025262111865
1202	23	0.9875250028970868
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2211	9	-0.3699722921827089
2211	10	-0.3845646333660184
2211	11	-0.24870911753615133
2211	12	-0.33784707898520594
2211	13	-0.3007207944055992
2211	14	-0.438562943283884
2211	15	-0.47939258111367167
2211	16	-0.38424941782747624
2211	17	-0.6613147494537799
2211	18	-0.5771275746812563
2211	19	-0.47131816515173597
2211	20	-0.4506786157860887
2211	21	-0.34272505667572517
2211	22	-0.4419348604532871
2211	23	-0.5107222502410309
2211	24	-0.4277813797990291
2211	25	-0.572337584146716
2212	1	-0.32474312432946206
2212	2	-0.18824827474884032
2212	3	-0.16805508956809234
2212	4	-0.23727745632587016
2212	5	-0.4433199288232714
2212	6	-0.26012459256164533
2212	7	-0.49539651141822816
2212	8	-0.2688481188923788
2212	9	-0.3449881543842892
2212	10	-0.6245936522525355
2212	11	-0.3594294451365201
2212	12	-0.16594486552690313
2212	13	-0.06325985575216464
2212	14	0.08020606825189702
2212	15	-0.12965474255499743
2212	16	-0.09192074580882803
2212	17	-0.3046264698622849
2212	18	-0.32610952502025725
2212	19	-0.054714455161033015
2212	20	-0.19725921005390035
2212	21	-0.2042881875178182
2212	22	-0.1640905450510246
2212	23	-0.4308727797132832
2212	24	-0.4850751957850079
2212	25	-0.35569387956224574
2213	1	-0.4230418890091556
2213	2	-0.0806696563368412
2213	3	-0.14963136891205053
2213	4	-0.35731631105588235
2213	5	-0.16075215613056315
2213	6	-0.07047662989560166
2213	7	-0.5214297975714928
2213	8	-0.44118564906816005
2213	9	-0.22326334801716285
2213	10	-0.35893072486712185
2213	11	-0.10817779691944196
2213	12	-0.2756223798114519
2213	13	-0.2563305090869079
2213	14	-0.4299129641105921
2213	15	-0.6196133152298557
2213	16	-1.11789137835396
2213	17	-0.7745119181820783
2213	18	-0.5243336749484442
2213	19	-0.46952358474103306
2213	20	-0.34401247160138837
2213	21	-0.3434017433853782
2213	22	-0.36538472177786474
2213	23	-0.46708158140607026
2213	24	-0.34111903812194555
2213	25	-0.3080291978758041
2214	1	-1.1587805828269317
2214	2	-0.5251019935870964
2214	3	-0.6549038098386291
2214	4	-0.6392197363264103
2214	5	-0.8528439305996898
2214	6	-1.4811458768474992
2214	7	-0.8708535919855294
2214	8	-0.6974387727242615
2214	9	-0.5192682645065787
2214	10	-0.9131530361982954
2214	11	-0.967655405390218
2214	12	-0.7023414076715611
2214	13	-1.1546925509991084
2214	14	-0.8906260875342369
2214	15	-0.8849337587599706
2214	16	-0.8404598663300646
2214	17	-0.9066876100355827
2214	18	-0.8988324371089078
2214	19	-0.9805551758006956
2214	20	-1.2312833550585012
2214	21	-1.3823559142653465
2214	22	-1.7964797739066043
2214	23	-1.8210484793685708
2214	24	-1.4547939686750837
2214	25	-2.059299000402625
2215	1	-1.5047131945547498
2215	2	-0.858036196074675
2215	3	-0.8609806850331516
2215	4	-0.9055293465902636
2215	5	-1.5277813952975023
2215	6	-1.2424310493527102
2215	7	-1.7308595285165183
2215	8	-1.4505914741340469
2215	9	-1.4672299594726326
2215	10	-1.4804764676284776
2215	11	-1.1029953438347349
2215	12	-1.3910294343225473
2215	13	-1.5291421250674162
2215	14	-1.8378241025427116
2215	15	-1.570515192979748
2215	16	-1.6377460647600586
2215	17	-1.790288375444625
2215	18	-1.4632754982839984
2215	19	-1.761703231730884
2215	20	-1.700769297583662
2215	21	-1.369292664273992
2215	22	-1.645286992267195
2215	23	-2.0331699534726155
2215	24	-2.0087789371480653
2215	25	-2.251187278347544
2216	1	-1.6119928029667854
2216	2	-1.0276638096804405
2216	3	-1.0308257613530465
2216	4	-1.1776872816179065
2216	5	-2.2609712102885418
2216	6	-2.4255970386071652
2216	7	-2.404620695949415
2216	8	-2.4226367655904752
2216	9	-4.270011367503173
2216	10	-2.394852550536399
2216	11	-2.435001696424621
2216	12	-2.807899818086085
2216	13	-2.80402098740964
2216	14	-2.955915872512975
2216	15	-2.9855549424852583
2216	16	-2.181871610884002
2216	17	-2.422980458110061
2216	18	-2.8958046072719412
2216	19	-2.9723054730418923
2216	20	-2.410204923685953
2216	21	-3.0786912829451936
2216	22	-2.524632545806732
2216	23	-3.70125523833115
2216	24	-2.9831225663011836
2216	25	-2.9977448823525243
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51822.0
3	1.0
4	892.0
5	7706.0
6	9819.0
7	7290.0
8	5880.0
9	4880.0
10	4124.0
11	3695.0
12	3393.0
13	3091.0
14	3190.0
15	3048.0
16	3186.0
17	3264.0
18	3653.0
19	4465.0
20	5941.0
21	7776.0
22	9790.0
23	11832.0
24	13638.0
25	15528.0
26	17623.0
27	19216.0
28	21708.0
29	25947.0
30	32258.0
31	41031.0
32	52567.0
33	67701.0
34	87580.0
35	115632.0
36	158580.0
37	246289.0
38	783232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.28997143934903	19.622083310879994	13.611952362989706	26.47599288678127
2	13.168685043755742	20.63511814535547	41.107882713276254	25.08831409761253
3	17.202545333913516	26.00404547824101	29.73173395487504	27.061675232970433
4	11.442812590189922	17.144969394656016	37.531822234857785	33.880395780296276
5	12.399586633774003	39.355415792248465	34.97376610773909	13.271231466238437
6	31.24391912640136	36.96400769301218	18.417168131104443	13.374905049482013
7	27.9713142910719	32.031369134250475	21.94885649978958	18.04846007488804
8	25.382873698143232	37.12623721173027	20.457729957210372	17.03315913291612
9	27.32374720629022	14.515496674475589	20.097800037698253	38.06295608153594
10	15.767574951842068	29.112816689578498	33.56306211188154	21.5565462466979
11	33.95188729585323	22.349894813698825	24.925357302276947	18.772860588170996
12	24.093895156474876	24.98251459270981	31.22977198167411	19.693818269141204
13	29.11253837845056	22.311408482404875	25.949701749228122	22.626351389916444
14	20.847076712013084	23.01694218035405	27.6541008303639	28.48188027726896
15	23.051240475845574	31.824905801811425	23.313331419841475	21.81052230250152
16	21.58636019501436	27.974620563094817	28.377831170635414	22.061188071255415
17	21.024459192052444	29.097581003405708	27.932639627391065	21.94532017715078
18	20.987121181934977	26.889042289347486	31.07549276905896	21.048343759658575
19	23.313693068043477	26.607237948373903	28.272290211437518	21.8067787721451
20	24.12038006709917	26.65062503668056	28.67905307360179	20.549941822618482
21	23.04838238120852	26.270428538276942	27.548650786343593	23.132538294170953
22	23.464718281989917	28.331282827167904	27.389360161312464	20.81463872952971
23	21.708391034573363	27.896620196977494	28.701027530760236	21.693961237688907
24	23.125203255534473	26.447717830706175	29.09467023606717	21.33240867769218
25	22.214403090991713	27.34096533187456	28.87811559774895	21.566515979384775
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	115.0
1	115.0
2	356.0
3	597.0
4	597.0
5	597.0
6	1425.5
7	2254.0
8	2254.0
9	2254.0
10	2652.0
11	3050.0
12	3050.0
13	3050.0
14	4529.0
15	6008.0
16	6008.0
17	6008.0
18	12224.5
19	18441.0
20	18441.0
21	18441.0
22	31513.5
23	44586.0
24	44586.0
25	44586.0
26	68797.5
27	93009.0
28	93009.0
29	93009.0
30	119676.5
31	146344.0
32	146344.0
33	146344.0
34	165809.0
35	185274.0
36	185274.0
37	185274.0
38	196102.5
39	206931.0
40	206931.0
41	206931.0
42	218931.0
43	230931.0
44	230931.0
45	230931.0
46	243151.0
47	255371.0
48	255371.0
49	255371.0
50	243753.0
51	232135.0
52	232135.0
53	232135.0
54	201180.5
55	170226.0
56	170226.0
57	170226.0
58	144353.0
59	118480.0
60	118480.0
61	118480.0
62	97036.0
63	75592.0
64	75592.0
65	75592.0
66	56707.0
67	37822.0
68	37822.0
69	37822.0
70	28184.5
71	18547.0
72	18547.0
73	18547.0
74	13081.0
75	7615.0
76	7615.0
77	7615.0
78	5180.0
79	2745.0
80	2745.0
81	2745.0
82	1833.5
83	922.0
84	922.0
85	922.0
86	576.5
87	231.0
88	231.0
89	231.0
90	134.0
91	37.0
92	37.0
93	37.0
94	21.0
95	5.0
96	5.0
97	5.0
98	2.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08442508027920581
2	0.0444739262185102
3	0.02046015976154222
4	0.004092031952308444
5	0.017175765694557813
6	0.05588854166442323
7	0.07704865425991295
8	0.05260414759743882
9	0.022506175737696445
10	0.04716605250292365
11	0.004684299734879403
12	2.6921262844134503E-4
13	0.005707307722956514
14	5.384252568826901E-4
15	0.015022064667027053
16	0.009368599469758807
17	0.005007354889009018
18	0.0059226778257095904
19	0.0015614332449598012
20	9.15322936700573E-4
21	5.384252568826901E-5
22	1.6152757706480703E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1857268.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.235935577871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.10057983994886	41.482507899001725
2	14.254093283492622	15.746763566559244
3	4.91225179435824	8.139984709663588
4	2.158154077750722	4.768306384230338
5	1.1002130825496745	3.0385649474822345
6	0.6556422180525787	2.172900679109081
7	0.4184646976025486	1.6180002354871355
8	0.2851596802660463	1.26008493828653
9	0.19348419622022073	0.9618552537980637
>10	0.817068921871095	7.803975137010909
>50	0.05481080704406077	2.141966945392723
>100	0.04147143352751882	4.621253354236734
>500	0.004987549239853799	1.935411965284446
>1k	0.003422827909703588	3.562597056419095
>5k	1.9559016626877646E-4	0.7458269280382467
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7981	0.4297171975180749	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5826	0.31368655465985523	No Hit
GTCCTACAGTGGACATTTCTAAATT	4687	0.25235991790091683	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4635	0.24956010656512684	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4566	0.2458449722926363	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4532	0.24401432641923512	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4300	0.23152286045955672	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2666	0.14354417348492518	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2588	0.1393444564812402	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2586	0.13923677142986365	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2246	0.1209303126958522	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.384252568826901E-5	0.0
5	0.0	0.0	0.0	5.384252568826901E-5	0.0
6	0.0	0.0	0.0	5.384252568826901E-5	0.0
7	0.0	0.0	0.0	5.384252568826901E-5	0.0
8	0.0	0.0	0.0	5.384252568826901E-5	0.0
9	0.0	0.0	0.0	1.0768505137653802E-4	0.0
10	0.0	0.0	0.0	1.0768505137653802E-4	0.0
11	0.0	0.0	0.0	1.0768505137653802E-4	0.0
12	0.0	0.0	0.0	1.0768505137653802E-4	5.384252568826901E-5
13	0.0	0.0	0.0	1.0768505137653802E-4	5.384252568826901E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	25	0.0059937737	19.021202	1
GGTATCA	1715	0.0	12.865653	1
AATCGCC	55	0.0030890978	12.080288	18
GGCGAGG	1080	0.0	11.776682	19
ATCGCCG	65	8.08409E-4	11.682036	19
TAGGACC	745	0.0	11.488077	4
ATAGGAC	250	0.0	11.413953	3
GTACTAA	140	8.20728E-9	10.869258	1
CCGTCTC	70	0.0014941999	10.85491	9
GCGCGCC	240	0.0	10.680124	11
TAGGACA	655	0.0	10.598458	4
GTGTCGA	245	0.0	10.46188	13
CCCGTAC	255	0.0	10.423051	16
GTATCAA	3680	0.0	10.389298	1
GATATAC	220	0.0	10.375201	1
GTAGGAC	2480	0.0	10.278694	3
GACGTGG	1255	0.0	10.22694	7
TGTAGGA	2570	0.0	10.214822	2
AGCACCG	75	0.0026259474	10.143547	5
GTCCTAC	2410	0.0	10.142011	1
>>END_MODULE