FastQCFastQC Report
Thu 26 May 2016
SRR1512916_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512916_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720233
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53610.3116438296440075No Hit
GTCCTACAGTGGACATTTCTAAATT37080.21555219554560343No Hit
GTCCTAAAGTGTGTATTTCTCATTT33440.19439227127953015No Hit
TATCAACGCAGAGTACTTTTTTTTT31380.18241714930477443No Hit
GGTATCAACGCAGAGTACTTTTTTT30440.1769527732580412No Hit
CTGTAGGACGTGGAATATGGCAAGA30070.1748019018353909No Hit
CTTTAGGACGTGAAATATGGCGAGG29170.1695700524289442No Hit
GTACTGGTTCACTATCGGTCAGTCA25060.14567794013950436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAC550.003072442312.0889283
TGCGCCT658.0371776E-411.69005210
GGTATCA17500.011.2694071
AGTCGTC1354.7384674E-911.2577428
GTATTAA1950.011.23731
GTCTTAG1701.0913936E-1111.2085591
TAGAAAT4000.010.9232094
TAGGACC5250.010.8553644
GGCGAGG9300.010.82586819
TGGCGAG17600.010.79399918
GATATAC4150.010.7899271
CGAAATC1158.8353227E-710.737370513
CCAACGA1158.838633E-710.73705819
GTCCTAA9150.010.7246921
ATATAAC1252.2450149E-710.6382573
ACGAAAT1252.2450149E-710.63825712
TAAGACC1355.700349E-810.5538264
TTAGGAC11850.010.4202333
TCCAACG1553.6689016E-910.41795118
GTGTTAT750.00258557310.1624271
Produced by FastQC (version 0.11.5)