FastQCFastQC Report
Thu 26 May 2016
SRR1512916_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512916_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1720233
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72600.42203585212003253No Hit
TATCAACGCAGAGTACTTTTTTTTT52440.30484242541562684No Hit
GGTATCAACGCAGAGTACTTTTTTT41110.23897925455447022No Hit
GTCCTACAGTGGACATTTCTAAATT33410.19421787629931528No Hit
CTTTAGGACGTGAAATATGGCGAGG31650.18398670412670842No Hit
GTCCTAAAGTGTGTATTTCTCATTT30630.17805727479940217No Hit
CTGTAGGACGTGGAATATGGCAAGA29800.17323234701345688No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24870.1445734385981434No Hit
GTACTGGTTCACTATCGGTCAGTCA22570.13120315678166852No Hit
CTGAAGGACCTGGAATATGGCGAGA17230.1001608503034182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC1102.7011993E-912.96933
CTAAGAC1155.2350515E-912.40541653
GGTATCA18600.012.0724151
GACCGTT802.8462666E-511.8833337
TAGGACC5750.011.5770414
TTTAGGC752.052245E-411.4129843
GTACTAG600.005808842411.1004841
AGAACCG600.005831560611.0946655
TACCGTA600.00584548111.0911127
TATGCGA600.00591167311.07434712
GACGTGG7800.010.8473517
GTCGAAA1502.1609594E-910.75824915
TCGAAAC1502.1627784E-910.75762416
GTATTAG1158.6428554E-710.7557471
GGCGAGG9300.010.61442419
CGCCCTA3050.010.58126916
AGGACCG1909.094947E-1210.5107355
GGACGTG18750.010.4987236
TAAGAGT1451.4202669E-810.4934754
AGGACCT10650.010.44728855
Produced by FastQC (version 0.11.5)