##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512916_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1720233
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.247067693736838	31.0	30.0	34.0	26.0	34.0
2	30.24305370260889	31.0	30.0	34.0	26.0	34.0
3	30.412209276301525	31.0	30.0	34.0	26.0	34.0
4	34.0206163932444	37.0	35.0	37.0	30.0	37.0
5	33.63353743359185	37.0	35.0	37.0	28.0	37.0
6	33.52399994651887	37.0	35.0	37.0	28.0	37.0
7	33.36614516754416	37.0	35.0	37.0	27.0	37.0
8	33.30649859641107	37.0	35.0	37.0	27.0	37.0
9	34.652179094343616	39.0	35.0	39.0	27.0	39.0
10	34.53811373226766	39.0	35.0	39.0	27.0	39.0
11	34.54981854202309	39.0	35.0	39.0	27.0	39.0
12	34.42569814670455	39.0	35.0	39.0	26.0	39.0
13	34.40587583193672	39.0	35.0	39.0	26.0	39.0
14	35.46387843972299	40.0	36.0	41.0	26.0	41.0
15	35.47237554447566	40.0	36.0	41.0	26.0	41.0
16	35.4632372475124	40.0	36.0	41.0	26.0	41.0
17	35.450725570315186	40.0	36.0	41.0	26.0	41.0
18	35.38534372959942	40.0	36.0	41.0	26.0	41.0
19	35.39108423103149	40.0	35.0	41.0	25.0	41.0
20	35.374286506537196	40.0	36.0	41.0	25.0	41.0
21	35.33681425713842	40.0	36.0	41.0	25.0	41.0
22	35.28763545403442	40.0	36.0	41.0	25.0	41.0
23	35.13358597352801	40.0	35.0	41.0	25.0	41.0
24	35.08865601345864	40.0	35.0	41.0	25.0	41.0
25	35.015260723402	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.048101845355795
1101	2	0.9066254161298133
1101	3	0.8865025138101856
1101	4	0.837694566649759
1101	5	0.9704059969527776
1101	6	1.004861902409722
1101	7	1.0071582661477123
1101	8	0.7025266552434957
1101	9	1.0570636143314118
1101	10	0.6805050799995414
1101	11	1.1847267138253912
1101	12	1.150038623773824
1101	13	1.303953366129214
1101	14	1.3761161621411873
1101	15	1.1586589072113327
1101	16	1.30664078143338
1101	17	1.4724913144715899
1101	18	1.289955141916792
1101	19	1.148961829464028
1101	20	1.3755283579325592
1101	21	1.3194270069667198
1101	22	1.2337794874180474
1101	23	1.415429440731863
1101	24	1.468778376766167
1101	25	1.4696140392047994
1102	1	0.8691430866158676
1102	2	0.9340793845457149
1102	3	0.6407985558883098
1102	4	0.5747774822145502
1102	5	0.8890220287441011
1102	6	0.7892317002283491
1102	7	0.8936567308317223
1102	8	0.9317807610647293
1102	9	0.986501777414432
1102	10	0.9619676338578103
1102	11	1.1589674643369392
1102	12	1.0803484552689753
1102	13	1.105870239621197
1102	14	1.0573541172565086
1102	15	1.2084106902660565
1102	16	1.2174521795937636
1102	17	1.2304204152317837
1102	18	0.5668393776567342
1102	19	0.9598220049241561
1102	20	1.2642876876538196
1102	21	1.105446890889496
1102	22	0.8163177985864039
1102	23	1.1072012734884282
1102	24	1.1566729986703592
1102	25	1.1657920349041078
1103	1	0.8572554142848894
1103	2	1.010811982854694
1103	3	0.667819416922903
1103	4	0.5506391211958643
1103	5	0.7482550246787767
1103	6	0.6859978899562478
1103	7	0.763131292330101
1103	8	0.8798317589461817
1103	9	0.8078225309310341
1103	10	0.8692541724783993
1103	11	0.9943550062466926
1103	12	1.0193889367459832
1103	13	0.9803529763039123
1103	14	1.1856842801876155
1103	15	1.1517223715547402
1103	16	1.0552826065483316
1103	17	1.0877800533605892
1103	18	0.9945650222875173
1103	19	1.0560170132902016
1103	20	0.9676098026408155
1103	21	1.0986499726476993
1103	22	1.0397364889027827
1103	23	1.1218551482713224
1103	24	1.0816708673966247
1103	25	0.9562525808919062
1104	1	0.7198451756357116
1104	2	0.8824705992920698
1104	3	0.7442161032310928
1104	4	0.5670490399066708
1104	5	0.7793248302618565
1104	6	0.6372846323934311
1104	7	0.48967816330175395
1104	8	0.6110182954424488
1104	9	0.713141770409564
1104	10	0.7884890538371749
1104	11	0.8315247630624611
1104	12	0.875355166163537
1104	13	0.6231853317026435
1104	14	0.8079536061737542
1104	15	1.0087104157994204
1104	16	0.9398382804845369
1104	17	0.9244684737658275
1104	18	0.4362980321621208
1104	19	0.8624913234813221
1104	20	0.963401153122426
1104	21	0.9113805379446376
1104	22	0.9221368343119778
1104	23	0.9320734207904806
1104	24	1.1231971287458933
1104	25	0.8866195781615573
1105	1	0.6594912170125902
1105	2	0.6109846601975129
1105	3	0.6869517164011825
1105	4	0.5390488578973063
1105	5	0.5738924210145413
1105	6	0.685133878052639
1105	7	0.6857674664203728
1105	8	0.7112650371586398
1105	9	0.9817234946606632
1105	10	0.6599553537739808
1105	11	0.97732009253847
1105	12	0.9387326127977857
1105	13	1.0989885627160447
1105	14	1.0525904361161267
1105	15	1.1119448139826034
1105	16	1.1135746513244058
1105	17	0.8521500030015758
1105	18	0.8189642933165473
1105	19	1.1218634729982995
1105	20	1.222379279638531
1105	21	1.0794402747080127
1105	22	1.0295774094318233
1105	23	1.3231366617300893
1105	24	1.2018853073987472
1105	25	0.8906940834723756
1106	1	0.3245708364361377
1106	2	0.5929578892186136
1106	3	-1.3645932485689123
1106	4	-0.09380627048135182
1106	5	0.2660888871193663
1106	6	0.3320066822556669
1106	7	0.5331247394586072
1106	8	0.49612296092094965
1106	9	0.7219439053799235
1106	10	0.5644119185450904
1106	11	0.652856752618753
1106	12	0.9085296198987436
1106	13	0.7374302683774872
1106	14	0.8991945198445706
1106	15	0.9654200360604435
1106	16	0.8874737468115939
1106	17	0.8677317020757798
1106	18	1.003934992322442
1106	19	1.0105049668220687
1106	20	0.9184932951304603
1106	21	0.8868990835566422
1106	22	0.8066125505634005
1106	23	0.9724061620578013
1106	24	1.0358965788285204
1106	25	1.0270429335381834
1107	1	0.7756737314642663
1107	2	0.800982561282293
1107	3	0.6284784005368671
1107	4	0.5533261131402654
1107	5	0.6727835063292247
1107	6	0.5564511167773034
1107	7	0.6246886678774715
1107	8	0.4634811479053056
1107	9	0.6154424335797941
1107	10	0.6175103393802033
1107	11	0.6506417343533499
1107	12	0.8909280513016427
1107	13	0.8340788904222904
1107	14	0.836847548111173
1107	15	0.7988037276318636
1107	16	0.8723868652070408
1107	17	0.9013863854663455
1107	18	0.9228964318039985
1107	19	0.8678428070936803
1107	20	0.848184016166158
1107	21	0.7991943822802199
1107	22	0.8395118603826575
1107	23	0.9175442106090941
1107	24	0.9681372128164085
1107	25	0.9345412999963187
1108	1	0.6490110206894073
1108	2	0.5965790517182796
1108	3	0.6407319904011857
1108	4	0.4329530553433969
1108	5	0.6315067852475451
1108	6	0.6230842443612659
1108	7	0.5640377516800328
1108	8	0.6083656853132027
1108	9	0.6804469807054332
1108	10	0.8137795464173863
1108	11	0.6341119114440019
1108	12	0.7001530189987761
1108	13	0.7044137867628706
1108	14	0.7918643284022338
1108	15	0.7346774314860625
1108	16	0.6994262456799802
1108	17	0.7824613130670741
1108	18	0.973447999218628
1108	19	0.9262330601202606
1108	20	1.0441156824350912
1108	21	0.9419354283126111
1108	22	0.9611243592808165
1108	23	1.0720680093832016
1108	24	1.097416402030582
1108	25	1.2110033137602443
1109	1	0.5226311717667151
1109	2	0.39695707840201777
1109	3	0.5216960190647733
1109	4	0.32364781576989543
1109	5	0.15172169547833647
1109	6	0.3174409412712791
1109	7	0.35189312919447957
1109	8	0.4591445621326429
1109	9	0.48788142465885187
1109	10	0.24206510811375637
1109	11	0.45735703653046755
1109	12	0.662229474228738
1109	13	0.43754622522290276
1109	14	0.6158267100188368
1109	15	0.6112490519632772
1109	16	0.45672705271481817
1109	17	0.6592885077102011
1109	18	0.7081803222580731
1109	19	0.8105027005645056
1109	20	0.7680518155664373
1109	21	0.7813423459099482
1109	22	0.772905746376864
1109	23	0.900187835840093
1109	24	0.9575501662512949
1109	25	0.9338425875561924
1110	1	0.4709901983384519
1110	2	0.2497741599196921
1110	3	0.3884001152871477
1110	4	0.39594430169483985
1110	5	0.4538271688810198
1110	6	0.5497700907759651
1110	7	0.5589781543680843
1110	8	0.5148090504154013
1110	9	0.3626437550993131
1110	10	0.5486831975500976
1110	11	0.5308919860736339
1110	12	0.6134871803734399
1110	13	0.5753764233234975
1110	14	0.6861350045220505
1110	15	0.5626447440731113
1110	16	0.5470109165167045
1110	17	0.5955743103793125
1110	18	0.6612659466293422
1110	19	0.6639781687790176
1110	20	0.6128272260533691
1110	21	0.7510308605574068
1110	22	0.7283793926407682
1110	23	0.7301784094378903
1110	24	0.7898698162808913
1110	25	0.8400957997184477
1111	1	0.08684267492893127
1111	2	0.013180671636384034
1111	3	0.005298905169926371
1111	4	0.26095488167243275
1111	5	0.29387222418667847
1111	6	0.2823372346444728
1111	7	0.198219490520124
1111	8	0.24025949277703518
1111	9	0.2352855221253023
1111	10	0.14781507853285092
1111	11	0.1256808120823294
1111	12	0.3885272396468977
1111	13	0.3134336678653753
1111	14	0.18570919793712903
1111	15	0.07854582804728949
1111	16	0.0016361944225309344
1111	17	0.28385732342704983
1111	18	0.09100109542549717
1111	19	-0.008903240520240274
1111	20	0.24555490220225096
1111	21	0.31638577563469994
1111	22	-0.00633830817094605
1111	23	0.17666330351265458
1111	24	0.18499913996424766
1111	25	0.1852826355794761
1112	1	0.15967990616811534
1112	2	-0.2516877209127628
1112	3	0.023828696797174587
1112	4	0.21551130464800394
1112	5	0.08634130006902296
1112	6	0.05457915433911609
1112	7	0.08411902518447789
1112	8	0.01215727797678312
1112	9	0.02570350157594703
1112	10	-0.2052011026436773
1112	11	-0.08415896657475486
1112	12	0.2808762112194714
1112	13	0.15284214042921462
1112	14	-0.0556977225938553
1112	15	-0.29635922647952384
1112	16	-0.3080999325699523
1112	17	-0.14424324610868666
1112	18	-0.006294363918648571
1112	19	-0.06605561669352511
1112	20	-0.16333446198527923
1112	21	-0.1293259795060635
1112	22	0.036337111302955805
1112	23	0.2210895586438255
1112	24	0.17600091410781715
1112	25	0.1631030988311224
1113	1	-0.8544608090476302
1113	2	-0.7927155098563041
1113	3	-0.7454174147043133
1113	4	-2.3832003537389497
1113	5	-0.8930236015205395
1113	6	-0.6633746776998422
1113	7	-0.7480459942337276
1113	8	-0.6769743085878872
1113	9	-0.6749810705434598
1113	10	-0.798910925072235
1113	11	-0.9139914494348815
1113	12	-0.5309058344370854
1113	13	-0.8646613448232827
1113	14	-0.944551665199441
1113	15	-0.995586027176266
1113	16	-1.098304097280483
1113	17	-1.3096111895591207
1113	18	-1.0653242619130054
1113	19	-0.927557103359554
1113	20	-0.6395754215522587
1113	21	-0.7965240001485796
1113	22	-0.6577438917951639
1113	23	-0.591868216358705
1113	24	-0.6032933618955454
1113	25	-0.7818260761331572
1114	1	-1.1267293546041586
1114	2	-2.321922918248859
1114	3	-1.6810195634388378
1114	4	-1.0981131264507127
1114	5	-1.1262159410159711
1114	6	-0.7540754450224227
1114	7	-0.6895013628897289
1114	8	-0.8786740408634302
1114	9	-1.2098928024258058
1114	10	-1.2096063147761171
1114	11	-1.1487299988656048
1114	12	-1.2490382395047703
1114	13	-1.2734361151180096
1114	14	-1.2524012537827005
1114	15	-1.5171864180655348
1114	16	-1.740853204191012
1114	17	-1.9099867555101184
1114	18	-1.5691412147113866
1114	19	-2.1665306720745363
1114	20	-1.5705377840037613
1114	21	-1.7959359315232248
1114	22	-1.3706545134594492
1114	23	-1.1733512826421943
1114	24	-1.2674015156376157
1114	25	-1.8643467915299112
1115	1	-1.3505351721665733
1115	2	-1.5081400991649048
1115	3	-1.1411929611211278
1115	4	-0.8531815636706099
1115	5	-1.15433947570029
1115	6	-1.063278345191165
1115	7	-1.0395404379744946
1115	8	-1.1891933726635315
1115	9	-2.1685957009996386
1115	10	-2.0306216928156005
1115	11	-2.3473950663777785
1115	12	-2.8725550609300363
1115	13	-2.921058276592209
1115	14	-2.450831870786267
1115	15	-2.647212989123183
1115	16	-2.347179540160184
1115	17	-2.369088379438743
1115	18	-2.66982702232162
1115	19	-3.207560787003132
1115	20	-3.3003066189920673
1115	21	-2.5465242906567482
1115	22	-2.560167717648298
1115	23	-2.4404443196215766
1115	24	-3.178467579644199
1115	25	-3.0331438211855115
1116	1	-2.046371606710103
1116	2	-2.2092282424067697
1116	3	-2.4295573769940013
1116	4	-2.026537844002796
1116	5	-2.5687542920108157
1116	6	-3.3260216776870735
1116	7	-2.5901404487749247
1116	8	-3.2376779080924614
1116	9	-3.827344987819128
1116	10	-2.7624711019616903
1116	11	-3.6984659524121213
1116	12	-5.818722552107726
1116	13	-4.930763988375709
1116	14	-4.5906780585869456
1116	15	-5.415286685617033
1116	16	-5.138290434740949
1116	17	-4.771661464299598
1116	18	-5.034687825920511
1116	19	-5.314333595279077
1116	20	-7.247901058179782
1116	21	-6.268156141592041
1116	22	-5.118514101500246
1116	23	-6.049779294933206
1116	24	-5.595487412519294
1116	25	-4.815630220862438
1201	1	0.7038392005504512
1201	2	0.9481022787344919
1201	3	1.0063657873756604
1201	4	0.9551854075826824
1201	5	0.43614972238086125
1201	6	0.8882418040667162
1201	7	1.150873054675671
1201	8	1.0523253812508173
1201	9	0.9023542997755456
1201	10	1.045127763161311
1201	11	0.756008518748267
1201	12	1.1997414317262738
1201	13	1.229442833100805
1201	14	1.269316187740941
1201	15	1.0791187521924144
1201	16	1.0713476089248175
1201	17	0.9264137877315974
1201	18	1.2753504568922907
1201	19	1.4247786248990622
1201	20	1.450242230756345
1201	21	1.1137317914467886
1201	22	1.1614580301549182
1201	23	1.1286228449613844
1201	24	0.8986978428655306
1201	25	1.13798789602604
1202	1	0.8654302555428259
1202	2	0.8882614853795658
1202	3	0.8963472405317212
1202	4	0.8224326565190978
1202	5	0.8941269810809516
1202	6	0.6957011547930065
1202	7	0.8704689254484492
1202	8	0.9235295740554221
1202	9	1.1750656687741454
1202	10	1.1754762363752036
1202	11	1.1858815549192556
1202	12	1.0403772623297058
1202	13	1.0985263176368463
1202	14	1.1806512818375268
1202	15	1.1068617395863427
1202	16	1.2123078802846479
1202	17	1.0706849081178547
1202	18	1.0590730468862901
1202	19	1.2216761053355967
1202	20	1.1427961683225263
1202	21	1.2767526925117068
1202	22	1.1637170398752374
1202	23	1.5034933163629205
1202	24	1.3250965711114375
1202	25	1.4278761247147145
1203	1	0.7405126305136918
1203	2	0.775383163257267
1203	3	0.6819181593542005
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2211	15	0.22491004932189185
2211	16	0.2532802120367066
2211	17	0.05688819279971824
2211	18	0.014900000527866553
2211	19	0.1366212157214548
2211	20	0.13291497617955628
2211	21	0.2586870939126129
2211	22	0.26948899268776216
2211	23	0.09819331919383956
2211	24	0.32551842949239074
2211	25	0.09932579086776627
2212	1	-0.003952826464175274
2212	2	0.14814080364152105
2212	3	0.17911088718247825
2212	4	0.13784463900415744
2212	5	-0.0962166349022553
2212	6	0.28364999573992833
2212	7	-0.08984570395796254
2212	8	0.12781524148468293
2212	9	0.17625260144492927
2212	10	-0.015529212445237306
2212	11	0.20248170005713462
2212	12	0.4248271118619016
2212	13	0.40928640553956797
2212	14	0.48142801549721526
2212	15	0.24570569996451752
2212	16	0.23732725250181375
2212	17	0.10512030536105499
2212	18	0.05078604287654542
2212	19	0.24842257733710937
2212	20	0.18916187888315505
2212	21	0.24012331480062699
2212	22	0.2482793302657953
2212	23	-0.004422643064074805
2212	24	0.08429734928007093
2212	25	0.3669402518784537
2213	1	-0.32320520404833175
2213	2	0.0239700774252114
2213	3	-0.05409886340070713
2213	4	-0.21212952145010888
2213	5	-0.10159144669402309
2213	6	3.6714399079329496E-4
2213	7	-0.5264625756112622
2213	8	-0.6458488327118062
2213	9	-0.16557531622831334
2213	10	-0.3885721529269972
2213	11	-0.10452456902647356
2213	12	-0.2945623403876283
2213	13	-0.2980983024522814
2213	14	-0.35463536829640674
2213	15	-0.575584435822158
2213	16	-1.0465648643344494
2213	17	-0.7850515129818874
2213	18	-0.30415670829262353
2213	19	-0.3554430825718953
2213	20	-0.346210889162883
2213	21	-0.25133779309052073
2213	22	-0.46469361192440317
2213	23	-0.523245727597974
2213	24	-0.16854633131440266
2213	25	-0.17683515856050747
2214	1	-1.1539955602606646
2214	2	-0.38640963906266634
2214	3	-0.5989734049506872
2214	4	-0.6533502921619032
2214	5	-0.8761611370479159
2214	6	-1.4474574040487624
2214	7	-0.9309679540213338
2214	8	-0.7123670893803364
2214	9	-0.5145840836686801
2214	10	-0.7670534966821876
2214	11	-0.7691995888210812
2214	12	-0.5026925557790847
2214	13	-1.0331188678782226
2214	14	-0.5460960264693924
2214	15	-0.6809262038990198
2214	16	-0.6676612733055833
2214	17	-0.8022073630336592
2214	18	-0.845286710009951
2214	19	-0.849797296457993
2214	20	-1.09400896881629
2214	21	-1.229164993636438
2214	22	-1.4389984483843534
2214	23	-1.5745144814697554
2214	24	-1.2614201859332468
2214	25	-1.6127017488259199
2215	1	-1.6647494658324575
2215	2	-1.1033402184119119
2215	3	-1.1356387927274945
2215	4	-1.2122204051129728
2215	5	-1.7739937222711504
2215	6	-1.5089196972358785
2215	7	-2.1159770782529392
2215	8	-1.6300362276230693
2215	9	-1.7650697380454616
2215	10	-1.8464133677895163
2215	11	-1.577325436439736
2215	12	-1.9436042018924482
2215	13	-2.199484631187275
2215	14	-2.2704534753755112
2215	15	-2.0559063224229703
2215	16	-1.993873800517406
2215	17	-2.203188809822329
2215	18	-1.797523209121806
2215	19	-2.1993351963184864
2215	20	-2.100432658551135
2215	21	-1.7339134793053859
2215	22	-1.8906026862044456
2215	23	-2.3750028069456093
2215	24	-2.2475550570102243
2215	25	-2.6451932407683074
2216	1	-3.044126432957409
2216	2	-2.1012057867747522
2216	3	-2.039983706865133
2216	4	-2.5833720905941426
2216	5	-3.5537059808566056
2216	6	-3.528444634929464
2216	7	-3.463472146422262
2216	8	-3.758488117888664
2216	9	-5.872973811721952
2216	10	-3.9592743094640355
2216	11	-3.7059677146690575
2216	12	-4.207603976556559
2216	13	-4.307035052701011
2216	14	-4.47497761500448
2216	15	-4.268049631876959
2216	16	-3.8403219956916743
2216	17	-4.173333369055939
2216	18	-4.509114654286424
2216	19	-4.547407166098438
2216	20	-4.0407901080409445
2216	21	-4.494342306025217
2216	22	-3.9116903700331527
2216	23	-5.442673991805538
2216	24	-4.572315194256472
2216	25	-4.603921824730445
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	46193.0
3	0.0
4	742.0
5	6666.0
6	8095.0
7	6334.0
8	5221.0
9	4234.0
10	3677.0
11	3256.0
12	3028.0
13	2816.0
14	2813.0
15	2745.0
16	2854.0
17	3124.0
18	3499.0
19	4198.0
20	5519.0
21	7103.0
22	9240.0
23	11149.0
24	12932.0
25	14661.0
26	16573.0
27	18004.0
28	20544.0
29	24486.0
30	30780.0
31	39355.0
32	49260.0
33	63525.0
34	82622.0
35	108643.0
36	148645.0
37	231773.0
38	715924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.636231137797715	19.344626654215507	13.665616463462529	26.353525744524255
2	13.656363583495681	20.643075223168854	40.05780582129045	25.642755372045013
3	17.639996255641975	25.617339401053883	29.401582979683372	27.341081363620773
4	11.901864885746027	17.06202740560611	36.47418639788531	34.56192131076255
5	13.075774560101957	38.974117761973645	34.20076467605922	13.749343001865178
6	32.18422962918154	36.27427257879136	17.855381265035852	13.686116526991245
7	28.43646724773722	31.788452778952546	21.430054839672348	18.345025133637886
8	26.111726677077975	36.35139025815965	19.96923139560922	17.567651669153165
9	27.473678885602364	14.410661134295102	19.568535489531737	38.5471244905708
10	16.19827337526986	28.91096087992258	32.60318571800789	22.287580026799674
11	34.68089336602823	22.1995109717431	24.02947191691868	19.09012374530999
12	24.293872672692224	24.73526765056725	30.592340085151505	20.378519591589022
13	29.45626518771728	21.740788540467637	25.592742451195832	23.210203820619252
14	21.590743942323453	22.16171941397989	27.143832104610237	29.103704539086415
15	23.832859979244127	30.52293756667897	23.059892615966906	22.584309838109995
16	22.55859448806309	27.611774999491306	27.109357967774848	22.72027254467076
17	21.73061752208675	28.370798111093865	27.362804036161986	22.535780330657403
18	21.766810006795914	26.637777729978357	29.81185954023804	21.783552722987693
19	23.90596896403786	26.063453160624533	27.640887851282237	22.38969002405537
20	24.486514390153726	26.186138388237673	27.831466500484826	21.495880721123772
21	24.028193986251797	25.741748899126566	26.870612855876413	23.35944425874522
22	24.025968741243865	27.269910511550254	26.864090797583103	21.84002994962278
23	22.81458383835213	27.002969946513062	27.671484037336803	22.510962177798007
24	23.764978348863206	26.05158719778077	28.040445683811438	22.14298876954459
25	23.018755609708457	26.61251505610871	27.969831976152054	22.39889735803078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	131.0
1	131.0
2	323.5
3	516.0
4	516.0
5	516.0
6	1220.0
7	1924.0
8	1924.0
9	1924.0
10	2227.5
11	2531.0
12	2531.0
13	2531.0
14	3643.0
15	4755.0
16	4755.0
17	4755.0
18	9887.5
19	15020.0
20	15020.0
21	15020.0
22	25435.0
23	35850.0
24	35850.0
25	35850.0
26	55746.5
27	75643.0
28	75643.0
29	75643.0
30	98859.5
31	122076.0
32	122076.0
33	122076.0
34	137950.0
35	153824.0
36	153824.0
37	153824.0
38	165961.5
39	178099.0
40	178099.0
41	178099.0
42	190968.0
43	203837.0
44	203837.0
45	203837.0
46	220680.0
47	237523.0
48	237523.0
49	237523.0
50	229942.5
51	222362.0
52	222362.0
53	222362.0
54	197648.0
55	172934.0
56	172934.0
57	172934.0
58	149537.0
59	126140.0
60	126140.0
61	126140.0
62	105220.5
63	84301.0
64	84301.0
65	84301.0
66	64957.5
67	45614.0
68	45614.0
69	45614.0
70	34062.5
71	22511.0
72	22511.0
73	22511.0
74	16043.0
75	9575.0
76	9575.0
77	9575.0
78	6550.5
79	3526.0
80	3526.0
81	3526.0
82	2340.5
83	1155.0
84	1155.0
85	1155.0
86	736.5
87	318.0
88	318.0
89	318.0
90	189.5
91	61.0
92	61.0
93	61.0
94	33.0
95	5.0
96	5.0
97	5.0
98	3.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08824385998873407
2	0.039703923828923175
3	0.018137077942348506
4	0.004301742845300608
5	0.01680004976070102
6	0.0586548450122745
7	0.07103688860753166
8	0.055108813747905085
9	0.024182770589798012
10	0.04702851299794853
11	0.005057454426231796
12	2.9065830035814917E-4
13	0.0054062443866615744
14	0.0011045015413609668
15	0.0138353350970479
16	0.008603485690601216
17	0.004883059446016906
18	0.004766796125873647
19	0.002034608102507044
20	0.001685818142077265
21	4.6505328057303865E-4
22	6.394482607879282E-4
23	0.0
24	0.0
25	5.813166007162983E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1720233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.98427492166545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.34188674581934	38.859666906141435
2	14.757690841720997	15.638510975333798
3	5.213809272781647	8.287497116945742
4	2.392188147172274	5.069934178165011
5	1.2924500094830136	3.4239763312478555
6	0.7785996811037252	2.47521237345125
7	0.47156903267741745	1.7490020290346888
8	0.32535693775535524	1.3791041150160748
9	0.2387181856914443	1.1383478981529003
>10	1.067888754170474	9.62904264661363
>50	0.06554882589151115	2.389621446167142
>100	0.04570413375847084	4.617270030028981
>500	0.005946529828381108	2.2091254986900095
>1k	0.002422660300451563	2.4041204122345134
>5k	2.202418454955966E-4	0.7295680427769234
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7260	0.42203585212003253	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5244	0.30484242541562684	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4111	0.23897925455447022	No Hit
GTCCTACAGTGGACATTTCTAAATT	3341	0.19421787629931528	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3165	0.18398670412670842	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3063	0.17805727479940217	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2980	0.17323234701345688	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2487	0.1445734385981434	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2257	0.13120315678166852	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1723	0.1001608503034182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATAC	110	2.7011993E-9	12.9693	3
CTAAGAC	115	5.2350515E-9	12.4054165	3
GGTATCA	1860	0.0	12.072415	1
GACCGTT	80	2.8462666E-5	11.883333	7
TAGGACC	575	0.0	11.577041	4
TTTAGGC	75	2.052245E-4	11.412984	3
GTACTAG	60	0.0058088424	11.100484	1
AGAACCG	60	0.0058315606	11.094665	5
TACCGTA	60	0.005845481	11.091112	7
TATGCGA	60	0.005911673	11.074347	12
GACGTGG	780	0.0	10.847351	7
GTCGAAA	150	2.1609594E-9	10.758249	15
TCGAAAC	150	2.1627784E-9	10.757624	16
GTATTAG	115	8.6428554E-7	10.755747	1
GGCGAGG	930	0.0	10.614424	19
CGCCCTA	305	0.0	10.581269	16
AGGACCG	190	9.094947E-12	10.510735	5
GGACGTG	1875	0.0	10.498723	6
TAAGAGT	145	1.4202669E-8	10.493475	4
AGGACCT	1065	0.0	10.4472885	5
>>END_MODULE