FastQCFastQC Report
Thu 26 May 2016
SRR1512915_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512915_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840258
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61410.33370320900656325No Hit
TATCAACGCAGAGTACTTTTTTTTT35530.19307075420946412No Hit
GGTATCAACGCAGAGTACTTTTTTT34440.18714767168516588No Hit
GTACTGGTTCACTATCGGTCAGTCA31900.17334525919735166No Hit
GTCCTACAGTGGACATTTCTAAATT29850.16220551683513942No Hit
CTGTAGGACGTGGAATATGGCAAGA28060.15247861984569555No Hit
GTCCTAAAGTGTGTATTTCTCATTT27650.1502506713732531No Hit
CTTTAGGACGTGAAATATGGCGAGG23960.13019913512127104No Hit
GTCCTACAGTGTGCATTTCTCATTT23120.12563455776309626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACT400.005283451614.2484284
TAGGACC6250.012.3106414
GGTATCA18650.012.0483131
ACCGACT658.0320716E-411.6910188
TGTAGGA17600.010.9561792
GTAGGAC17600.010.7942653
GACGTGA9250.010.5772137
AGGACGT17600.010.3085225
GATATAC2600.010.2536331
CTGTAGG17950.010.2373111
AAATGTC3250.010.2296417
GTATCAA37350.010.1967651
TAGGACG18300.010.1737414
ACGTGAA11350.010.12663658
GTGCTAG1605.9499143E-910.1163061
GTCCTAC16600.010.0947981
TCCTACA18350.010.0942552
TCTATAC1606.182745E-910.0926373
GGACGTG17950.010.0546026
TGGCGAG16250.010.05427618
Produced by FastQC (version 0.11.5)