##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512915_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1840258
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.004287985706352	31.0	30.0	34.0	23.0	34.0
2	29.083156818228748	31.0	30.0	34.0	22.0	34.0
3	29.2469947148715	31.0	30.0	34.0	23.0	34.0
4	32.67033752875955	35.0	33.0	37.0	25.0	37.0
5	32.8661296405178	35.0	35.0	37.0	26.0	37.0
6	32.73083828463183	36.0	33.0	37.0	25.0	37.0
7	32.71311142242012	37.0	33.0	37.0	25.0	37.0
8	32.62167370010075	37.0	33.0	37.0	25.0	37.0
9	33.92028183004774	39.0	34.0	39.0	23.0	39.0
10	33.80082629718224	38.0	34.0	39.0	22.0	39.0
11	33.86359249626955	39.0	34.0	39.0	23.0	39.0
12	33.76469603718609	39.0	34.0	39.0	22.0	39.0
13	33.7314088568016	38.0	34.0	39.0	22.0	39.0
14	34.589947713853164	39.0	34.0	41.0	18.0	41.0
15	34.76344458222706	39.0	34.0	41.0	21.0	41.0
16	34.70688620834687	39.0	34.0	41.0	19.0	41.0
17	34.46353228732058	39.0	34.0	41.0	17.0	41.0
18	34.466243863632165	39.0	34.0	41.0	17.0	41.0
19	34.588203393219864	39.0	34.0	41.0	18.0	41.0
20	34.55775874904497	39.0	34.0	41.0	18.0	41.0
21	34.4561561476706	39.0	34.0	41.0	18.0	41.0
22	34.4663193965194	39.0	34.0	41.0	18.0	41.0
23	34.132630315966566	39.0	34.0	41.0	10.0	41.0
24	34.19869333539101	39.0	34.0	41.0	10.0	41.0
25	33.91699805136019	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6743165001678264
1101	2	0.3158349793820783
1101	3	0.7168853390574839
1101	4	0.7658458759747404
1101	5	0.7337789327498854
1101	6	0.5779583699075204
1101	7	0.4428801877140103
1101	8	0.665451780627599
1101	9	0.7082249099431124
1101	10	0.7086842861733018
1101	11	0.59585546929938
1101	12	0.5574755961104643
1101	13	1.0362973107632243
1101	14	1.0039154800469774
1101	15	0.9211729052968209
1101	16	0.7073554657978676
1101	17	0.10369022242812775
1101	18	0.6104541221929551
1101	19	0.020003774108765526
1101	20	-0.4674163485444751
1101	21	0.5891633880524481
1101	22	0.5937614466177692
1101	23	0.20320106886626377
1101	24	0.7540693326403414
1101	25	0.3170111092310819
1102	1	0.9243477766519455
1102	2	0.9399602467455708
1102	3	0.8819444617016607
1102	4	1.0402284176430925
1102	5	0.9257025374151198
1102	6	0.7142522074312296
1102	7	0.8735382045831344
1102	8	0.8115785411909826
1102	9	1.1085265766770434
1102	10	1.1777589058108973
1102	11	0.9225706313317517
1102	12	1.1253869323225771
1102	13	1.0093965076638298
1102	14	1.1479991799541551
1102	15	1.0812156834557456
1102	16	1.132252556075926
1102	17	1.2334815780114852
1102	18	1.2930078975672643
1102	19	1.0381279921966637
1102	20	1.3201638605035058
1102	21	1.3381907902882162
1102	22	1.142335844293214
1102	23	1.1092616443535732
1102	24	0.5639426124337135
1102	25	0.9108950432074323
1103	1	0.8108559222084182
1103	2	0.7744451732417375
1103	3	0.8959332994564875
1103	4	0.9654632422576839
1103	5	0.870883787228756
1103	6	0.5637788308272178
1103	7	0.39553021309149017
1103	8	0.5131934884610487
1103	9	0.7884559056501743
1103	10	0.9522094907602536
1103	11	0.7424723280327754
1103	12	0.8541003916246126
1103	13	0.954090713832386
1103	14	1.091617665745403
1103	15	0.9523854330755555
1103	16	1.0048128650007513
1103	17	0.6329003560289195
1103	18	1.0916277749117782
1103	19	1.009343464281109
1103	20	1.1359311530378235
1103	21	1.0570826169006722
1103	22	0.9491186725258487
1103	23	0.3400872827286392
1103	24	0.5912376813652997
1103	25	0.9167999504206463
1104	1	1.1557222931811744
1104	2	1.1046024175597466
1104	3	1.0408713390277917
1104	4	1.2803923720083148
1104	5	1.0709298598992518
1104	6	0.9175501470551595
1104	7	1.1255304690507728
1104	8	1.0195968583478034
1104	9	1.2631849239975779
1104	10	1.1822141345929182
1104	11	1.0561873575960732
1104	12	1.3635811233756314
1104	13	1.2964151653786828
1104	14	1.7277450993392094
1104	15	1.3710700948984993
1104	16	1.4108953107038644
1104	17	1.6130207300219936
1104	18	1.3167338456564863
1104	19	0.8866970403369692
1104	20	1.0397581099379352
1104	21	1.1026888320795507
1104	22	0.9415160724670955
1104	23	1.3795096435751546
1104	24	1.1460104078069904
1104	25	0.7131749575814155
1105	1	0.6622419298925593
1105	2	0.7426818072521613
1105	3	0.7912341413856048
1105	4	0.8652618297948322
1105	5	0.6168266543796861
1105	6	0.7223241182146651
1105	7	0.7635255740554427
1105	8	0.7195880336689626
1105	9	0.5039019921871315
1105	10	0.745112663392888
1105	11	0.7444770087691239
1105	12	0.9067890922407287
1105	13	0.7381796689184625
1105	14	0.8748009883715682
1105	15	0.7936987326022091
1105	16	0.7096521115596772
1105	17	1.1360273881521366
1105	18	0.9310172187008803
1105	19	0.6984968966207745
1105	20	0.8418457768189143
1105	21	0.8398383887745098
1105	22	0.7307593292418417
1105	23	0.9750411143609625
1105	24	1.0912797496120135
1105	25	1.282394477771014
1106	1	0.8410391157535528
1106	2	0.8682761720742178
1106	3	0.7734866428281215
1106	4	0.8970282738523068
1106	5	0.6561899282614405
1106	6	0.8436750222356935
1106	7	0.7964486199288103
1106	8	0.7327151624071107
1106	9	0.890428659147048
1106	10	0.6114151859233345
1106	11	0.45981668831883127
1106	12	0.7150303761118693
1106	13	0.8224063283746972
1106	14	0.9797421189778746
1106	15	0.7648388866950313
1106	16	0.8962772751097319
1106	17	1.1290920897169556
1106	18	0.8802397252579723
1106	19	0.7977326424305744
1106	20	0.9218893499164125
1106	21	1.003820548989303
1106	22	0.9235616607303001
1106	23	1.1300602573716318
1106	24	0.9814500141641957
1106	25	1.2122040136554304
1107	1	0.9111644718520893
1107	2	0.9365986916471911
1107	3	0.9539848929168038
1107	4	0.9906765610489785
1107	5	0.9433829816741408
1107	6	0.9528080059609181
1107	7	0.6941851711630065
1107	8	0.9663540618669586
1107	9	1.0286143830522363
1107	10	1.08254173968853
1107	11	0.9990468190676296
1107	12	1.0029336049357198
1107	13	0.8051320718833281
1107	14	1.1159463762404869
1107	15	1.0546113348582011
1107	16	1.1059738247845345
1107	17	1.4129491049983969
1107	18	1.3749505237020045
1107	19	1.0461752714458683
1107	20	1.314727634019647
1107	21	0.9295580952415321
1107	22	1.0261182801408566
1107	23	1.3068268277590818
1107	24	1.0799816625327452
1107	25	1.4262027731416822
1108	1	0.9151987416836818
1108	2	0.8425946558651312
1108	3	0.881485524271131
1108	4	1.019499122157157
1108	5	0.8883492410053009
1108	6	1.0158929538571755
1108	7	0.9619503652822132
1108	8	0.934413174546286
1108	9	1.000622266635034
1108	10	1.0376844653175823
1108	11	0.8341796431065305
1108	12	1.0631996473555176
1108	13	1.1222017642116597
1108	14	1.2374715282221018
1108	15	1.1000135921927594
1108	16	1.0426998486458245
1108	17	1.334159458069422
1108	18	1.310709769887623
1108	19	1.2886721007152104
1108	20	1.445266234226544
1108	21	1.3201102194013288
1108	22	1.170820039522674
1108	23	1.514516868902092
1108	24	1.3872646938803754
1108	25	1.5779089304255152
1109	1	0.43464636060851447
1109	2	0.4775233612818468
1109	3	0.5690244890354741
1109	4	0.5466983694292793
1109	5	0.39097720428426186
1109	6	0.42126693731999865
1109	7	0.4085985667561758
1109	8	0.3002226701154669
1109	9	0.5248718179359173
1109	10	0.5731150046681961
1109	11	0.42184172961958666
1109	12	0.35504412073608194
1109	13	0.43764714355464207
1109	14	0.6227581108983102
1109	15	0.5423247909307847
1109	16	0.1271426513646361
1109	17	0.5174011330777333
1109	18	0.5647420762241069
1109	19	0.6081889238241658
1109	20	0.3938825879626009
1109	21	0.34675728358763536
1109	22	0.5793257080037861
1109	23	0.8098197097269448
1109	24	0.6286696539279646
1109	25	0.21278088656949024
1110	1	0.7323182195582909
1110	2	0.7104094302421089
1110	3	0.67465100367804
1110	4	0.6617464934710853
1110	5	0.6408271566407393
1110	6	0.729821556928826
1110	7	0.5937830845592629
1110	8	0.6872311280880794
1110	9	0.7542647460227485
1110	10	0.6993160657906259
1110	11	0.7150368649933867
1110	12	0.6976408561345409
1110	13	0.861900735858427
1110	14	0.9729477783727916
1110	15	0.8055657021687637
1110	16	0.9663608215449528
1110	17	1.0233837728826956
1110	18	0.3027398513484769
1110	19	0.8265864421902691
1110	20	0.8249491819173613
1110	21	0.8473765134397695
1110	22	0.9055830029938221
1110	23	1.0706776513271308
1110	24	0.8903742767553311
1110	25	1.0296477376451634
1111	1	0.3385459260076935
1111	2	0.42133326008753613
1111	3	0.4534956852307346
1111	4	0.36715510109629435
1111	5	0.3606132181266304
1111	6	0.3483371666627946
1111	7	0.10910670174386183
1111	8	0.39010537981651083
1111	9	0.4074763075054051
1111	10	0.44105945938581215
1111	11	0.21717648884927598
1111	12	-0.13470652806801553
1111	13	0.17142521930508536
1111	14	0.22823124826805952
1111	15	0.31234594253642456
1111	16	0.4509709325735045
1111	17	0.0069193829417812935
1111	18	0.3159684047808753
1111	19	0.3713749732380265
1111	20	0.5686004754619276
1111	21	0.41116629114737435
1111	22	0.1643431840397085
1111	23	0.3921261684362207
1111	24	0.4120809014445612
1111	25	0.45908664295801316
1112	1	0.23262634217861944
1112	2	0.317392507199731
1112	3	0.31298569901088413
1112	4	0.47973436770625
1112	5	0.2832490979652107
1112	6	0.18534881404325887
1112	7	0.06644703992831325
1112	8	0.28969318318997495
1112	9	0.26283740455733806
1112	10	0.2675592724069702
1112	11	0.24400368006243411
1112	12	0.2822807904138074
1112	13	0.3355829588049275
1112	14	0.1859254903451628
1112	15	0.03543130082822188
1112	16	0.25580136748789073
1112	17	0.5298372236859379
1112	18	0.2619683222868545
1112	19	0.47510789589396296
1112	20	0.2690597985050829
1112	21	0.32837009945406237
1112	22	0.03684924974277237
1112	23	0.2942390407616884
1112	24	0.26372726098585275
1112	25	0.18721849300941074
1113	1	-0.13874344284132079
1113	2	-0.051863561115368384
1113	3	-0.04259866482610164
1113	4	0.01030466022253762
1113	5	-0.08377831463935337
1113	6	-0.26484219368621353
1113	7	-0.3414998197058452
1113	8	-0.21712397694815877
1113	9	-0.20281765953914999
1113	10	-0.1269213797592741
1113	11	-0.13289827023190526
1113	12	-0.28308026202810055
1113	13	-0.1298858808823269
1113	14	0.05125064466528784
1113	15	-0.17608944206320842
1113	16	-0.268430329114004
1113	17	-0.10713942070810134
1113	18	-0.2127587962596138
1113	19	-0.7196301267650398
1113	20	-0.46430632146987705
1113	21	-0.20839575376952268
1113	22	-0.6897946798987675
1113	23	-4.2245440825699525
1113	24	-1.6614172603013202
1113	25	-1.081013155294876
1114	1	-0.45644257661849963
1114	2	-0.7074622079252713
1114	3	-0.5828480460251875
1114	4	-0.39979715394224513
1114	5	-0.5798351202679299
1114	6	-1.0155872654316767
1114	7	-1.0062768544630636
1114	8	-0.7882651600443111
1114	9	-1.0603661938129463
1114	10	-0.6912365537225682
1114	11	-0.7303207699709944
1114	12	-1.6322193558712002
1114	13	-0.957063941953102
1114	14	-2.9597551012728758
1114	15	-1.6356301017630699
1114	16	-1.6097590920832232
1114	17	-1.2859359636933547
1114	18	-1.4114162887227408
1114	19	-1.2792513733461277
1114	20	-0.9288741847803479
1114	21	-0.8453774526217117
1114	22	-1.0842696787665105
1114	23	-5.170538565051537
1114	24	-2.380846673676082
1114	25	-2.260962873413092
1115	1	-1.8612034408430063
1115	2	-1.6677637309722044
1115	3	-1.405069939037837
1115	4	-1.3247889751419422
1115	5	-1.403681539064479
1115	6	-1.4610336180571046
1115	7	-2.1394090818991423
1115	8	-2.3524238670407946
1115	9	-3.7875922780749605
1115	10	-4.351464621883768
1115	11	-3.0412219688288005
1115	12	-3.2820147315466457
1115	13	-2.6120477016334043
1115	14	-3.2522203583464595
1115	15	-2.966753739298394
1115	16	-3.1777057255655663
1115	17	-3.0788618261092644
1115	18	-5.765131439317603
1115	19	-3.492922994205344
1115	20	-3.917409420286589
1115	21	-3.5004199762106083
1115	22	-2.9997619931633004
1115	23	-3.3894022735232916
1115	24	-3.0885319065280683
1115	25	-3.514458893886637
1116	1	-2.0882915850169574
1116	2	-1.7694104266666706
1116	3	-2.3004683819530243
1116	4	-2.2539649762754834
1116	5	-2.2344199863453262
1116	6	-4.605726088062351
1116	7	-3.0997558836714454
1116	8	-3.4526191728092073
1116	9	-4.694427214064451
1116	10	-3.8690348649437105
1116	11	-3.4751511762320355
1116	12	-3.3551927393512173
1116	13	-4.7270353131204494
1116	14	-7.74589206849738
1116	15	-5.821842986375508
1116	16	-4.553629885655024
1116	17	-5.661832694913574
1116	18	-4.216681056556592
1116	19	-3.9871921102361156
1116	20	-4.5844601882978
1116	21	-5.437894174786791
1116	22	-4.962085212721451
1116	23	-4.178652797210816
1116	24	-5.139588663916179
1116	25	-5.043747483051376
1201	1	0.5889177629413638
1201	2	0.6418454517810943
1201	3	1.0527801840000528
1201	4	1.0470562875172007
1201	5	0.5687500258857696
1201	6	0.8726225177889546
1201	7	1.0594898436209021
1201	8	1.009434081512552
1201	9	1.4224378318185131
1201	10	1.3545668330201721
1201	11	1.4169081609341418
1201	12	1.3805851125544137
1201	13	1.3522844548157664
1201	14	1.4051804089208773
1201	15	1.5262555687854231
1201	16	1.4048507113794244
1201	17	1.6642362832339757
1201	18	1.532201466057849
1201	19	1.5388577690880965
1201	20	1.5195306808948814
1201	21	1.682561973137524
1201	22	1.4108513621792937
1201	23	1.7545358559951012
1201	24	1.6573534313293905
1201	25	1.8520760228908415
1202	1	1.1982118506772679
1202	2	1.0758444164226866
1202	3	1.219686821015035
1202	4	1.4571166630031058
1202	5	1.316521769178145
1202	6	1.458491039523821
1202	7	1.426962558251006
1202	8	1.4407200395407145
1202	9	1.6483866091460015
1202	10	1.6030586098851174
1202	11	1.5841912028048952
1202	12	1.821733809013672
1202	13	1.7052021131903743
1202	14	2.175890915970811
1202	15	1.8759625133447386
1202	16	1.6512926011229467
1202	17	2.0523526967279366
1202	18	2.0036258802992393
1202	19	2.0575229531705332
1202	20	1.7809149367080508
1202	21	1.926823973952331
1202	22	2.069317255084023
1202	23	2.0800223978673884
1202	24	2.0237697127998473
1202	25	2.3211874832056765
1203	1	1.048807027801928
1203	2	1.0965782222879135
1203	3	1.070472019519812
1203	4	1.2535809623291243
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2211	19	-0.17246534467253127
2211	20	-0.12711450985813144
2211	21	0.08357060053042176
2211	22	0.020309403921558555
2211	23	0.1833855369420192
2211	24	-0.20499731724196835
2211	25	0.32787748177187126
2212	1	-0.08138666426359364
2212	2	0.12635720974357412
2212	3	-0.34676587433787986
2212	4	-0.08571449135303766
2212	5	-0.25776819789461314
2212	6	-0.3139382857678541
2212	7	-0.36677350805044284
2212	8	-0.18804376426616187
2212	9	-0.1508827235382313
2212	10	-0.32371760231254854
2212	11	-0.6267159724582285
2212	12	-0.08100829303919
2212	13	0.08087562224163491
2212	14	0.06479415818543544
2212	15	-0.3525183520113373
2212	16	-0.4732535787453429
2212	17	-0.32696620119286735
2212	18	-0.16475547499160115
2212	19	-0.36609422251725476
2212	20	-0.13751214756684504
2212	21	-0.0351514564754396
2212	22	-0.043234052124354605
2212	23	-0.06314348553473792
2212	24	-0.12098025582449878
2212	25	0.04059052245715833
2213	1	-0.25729057936692357
2213	2	-0.14955133422465394
2213	3	-0.37937517017944344
2213	4	-0.2613881380503429
2213	5	-0.23288287978468247
2213	6	0.001933575033483237
2213	7	-0.18017292058404877
2213	8	-0.058322645152308894
2213	9	-0.5908116880846492
2213	10	-0.029077898353129683
2213	11	-0.34587345795543456
2213	12	-0.3982328632420291
2213	13	-0.3329803796456261
2213	14	-0.40088316105797617
2213	15	-0.28896603420709965
2213	16	-0.2164141958928667
2213	17	-0.18260612061772719
2213	18	-0.23014559314044192
2213	19	-0.18914305610204707
2213	20	-0.05717056083425831
2213	21	-0.20849739504593856
2213	22	-0.3488957413122904
2213	23	-0.24174447808555044
2213	24	-0.03389051247273045
2213	25	0.0717591134706268
2214	1	-2.9654557815393083
2214	2	-1.5782445963480782
2214	3	-1.7784323606358754
2214	4	-3.728494092858515
2214	5	-2.0942060368374094
2214	6	-1.7398755415186073
2214	7	-1.5870029370032235
2214	8	-1.498934184284682
2214	9	-1.3493212118082525
2214	10	-1.3796638764969842
2214	11	-1.536260601680361
2214	12	-1.3400949060762315
2214	13	-1.8345002059511941
2214	14	-1.6846410473747255
2214	15	-1.955331141352751
2214	16	-1.8123298506451064
2214	17	-3.983917730117991
2214	18	-3.5277457471078044
2214	19	-2.4456291118310105
2214	20	-2.3577802956615557
2214	21	-2.354873207735011
2214	22	-3.2196656382864717
2214	23	-2.1359780393627616
2214	24	-3.1940423146727817
2214	25	-3.141698235764647
2215	1	-1.8690439012160915
2215	2	-3.4899829671714038
2215	3	-2.198478950138675
2215	4	-3.94375069910442
2215	5	-2.3803988009052546
2215	6	-2.232211568307573
2215	7	-2.372595861222486
2215	8	-1.684479063079177
2215	9	-4.073459669986896
2215	10	-2.342021742115385
2215	11	-2.00477337739558
2215	12	-1.9264252547695442
2215	13	-2.0932830551904757
2215	14	-1.7916716945320417
2215	15	-1.989816108958486
2215	16	-2.0537471906756792
2215	17	-2.3291939913103903
2215	18	-2.0241321487966744
2215	19	-2.0699910540309148
2215	20	-1.905746372679829
2215	21	-2.505034815072861
2215	22	-2.4533287379443287
2215	23	-2.4758186159078583
2215	24	-2.189078348741358
2215	25	-3.093265535757368
2216	1	-3.3765604087790493
2216	2	-3.4915614706233207
2216	3	-4.605691553393889
2216	4	-5.358195959465331
2216	5	-5.007800470683218
2216	6	-4.027010543201921
2216	7	-5.654390061530961
2216	8	-3.669125333800263
2216	9	-4.35144068960723
2216	10	-3.330022609149509
2216	11	-3.7057380265859052
2216	12	-4.0112795841894275
2216	13	-3.5814085215162272
2216	14	-4.529945352914698
2216	15	-3.9851624360073004
2216	16	-4.253690715493793
2216	17	-4.018071771938963
2216	18	-3.5596628533061363
2216	19	-4.199668688384335
2216	20	-4.935285252082458
2216	21	-4.357685462251904
2216	22	-4.026523080399215
2216	23	-4.118518719421115
2216	24	-4.501136168710865
2216	25	-4.507898063692675
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	86413.0
3	4.0
4	779.0
5	4949.0
6	6078.0
7	5527.0
8	4939.0
9	4538.0
10	3981.0
11	3785.0
12	3737.0
13	3519.0
14	3459.0
15	3524.0
16	3668.0
17	3911.0
18	4331.0
19	5403.0
20	6943.0
21	9013.0
22	11441.0
23	13683.0
24	15853.0
25	18211.0
26	20124.0
27	21671.0
28	24775.0
29	29346.0
30	35308.0
31	43697.0
32	54684.0
33	69949.0
34	90302.0
35	119253.0
36	161612.0
37	246058.0
38	695790.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.68043229120664	18.32123498065027	12.70626900760795	24.29206372053514
2	15.243084614966579	19.86299657627303	41.07564806260529	23.8182707461551
3	19.508905256419105	25.07829767299805	28.62121642764795	26.791580642934893
4	12.650781159990524	16.116655543986035	37.406281452368475	33.82628184365497
5	13.511975670411463	38.49898699175681	34.18718044589754	13.801856891934195
6	32.31325609155897	35.47317158061034	18.260292634114904	13.953279693715789
7	29.24206003915597	31.362519034152708	21.099358277137263	18.296062649554056
8	26.253990581981746	35.63028757212149	20.336370228096197	17.779351617800565
9	27.986353691306924	14.58494109278152	19.708615524008575	37.720089691902984
10	16.75653508566779	28.11356815514295	32.681737439179734	22.448159320009527
11	34.710705146856874	22.433084334269683	23.858715012549663	18.997495506323776
12	24.397864124880268	24.575618018413955	30.29104752414608	20.735470332559693
13	29.523791913349072	21.63651729940274	25.566558295000803	23.273132492247385
14	21.923207452351377	22.262637953126937	26.918617172866444	28.89553742165524
15	24.125236712810935	30.285208345343463	23.111165013166726	22.47838992867888
16	22.75403573615678	27.689756555997775	26.972401122968538	22.58380658487691
17	22.293839316261632	28.143886175598226	26.971178924340254	22.591095583799888
18	22.174536917672683	26.694070305182322	29.22985876084206	21.901534016302936
19	23.992435310153372	26.402225241522903	27.44098312684725	22.164356321476475
20	24.763688756104603	26.07693692547092	27.67741903902542	21.481955279399052
21	24.242043557920876	25.90471031812716	26.69544641512122	23.15779970883074
22	24.371601404014157	27.247186885859392	26.570037798289096	21.811173911837354
23	23.177357410963946	26.864169811803084	27.46534910878283	22.493123668450135
24	23.755283142729564	26.180402701792797	27.70583113196721	22.358483023510427
25	23.219632219491356	26.70446974439412	27.631562487932733	22.444335548181794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	267.0
1	267.0
2	612.5
3	958.0
4	958.0
5	958.0
6	1619.0
7	2280.0
8	2280.0
9	2280.0
10	2591.0
11	2902.0
12	2902.0
13	2902.0
14	4130.0
15	5358.0
16	5358.0
17	5358.0
18	10722.5
19	16087.0
20	16087.0
21	16087.0
22	26537.5
23	36988.0
24	36988.0
25	36988.0
26	56391.5
27	75795.0
28	75795.0
29	75795.0
30	98974.5
31	122154.0
32	122154.0
33	122154.0
34	141533.0
35	160912.0
36	160912.0
37	160912.0
38	174801.0
39	188690.0
40	188690.0
41	188690.0
42	203382.0
43	218074.0
44	218074.0
45	218074.0
46	235767.0
47	253460.0
48	253460.0
49	253460.0
50	246510.0
51	239560.0
52	239560.0
53	239560.0
54	214337.5
55	189115.0
56	189115.0
57	189115.0
58	163592.5
59	138070.0
60	138070.0
61	138070.0
62	116223.5
63	94377.0
64	94377.0
65	94377.0
66	73343.5
67	52310.0
68	52310.0
69	52310.0
70	38954.5
71	25599.0
72	25599.0
73	25599.0
74	18268.5
75	10938.0
76	10938.0
77	10938.0
78	7627.0
79	4316.0
80	4316.0
81	4316.0
82	2886.5
83	1457.0
84	1457.0
85	1457.0
86	964.5
87	472.0
88	472.0
89	472.0
90	288.0
91	104.0
92	104.0
93	104.0
94	57.0
95	10.0
96	10.0
97	10.0
98	7.5
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3327794254935993
2	0.008585752649900177
3	0.008966134096414741
4	0.009455195956219183
5	0.010542000089117938
6	0.07841291818864529
7	0.07922802128831935
8	0.10145316580609894
9	0.08254277389366056
10	0.08933529972427778
11	0.08493374298603783
12	0.09569310390173552
13	0.08281447492688525
14	0.08640092856545115
15	0.08324919658004475
16	0.08477072236610302
17	0.09656254720805453
18	0.0986818152672071
19	0.09324779460271332
20	0.09167192861001011
21	0.07960840273483391
22	0.09862747506056216
23	0.09265005232961901
24	0.08841151621131385
25	0.08732471207841509
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1840258.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.37589640983618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.64626408941972	40.04581627097724
2	14.430861497071016	15.69382059738854
3	5.216917222624234	8.51023651428317
4	2.4375417312326593	5.301740666886393
5	1.3250865859516108	3.602638546588414
6	0.7831063054032062	2.5549264404296563
7	0.49441309472398576	1.8818908655664577
8	0.3227886388200024	1.4041537269398783
9	0.24125886286560402	1.1806800241621462
>10	1.00196766518096	9.191520808181833
>50	0.05279493090802512	1.9939073720284421
>100	0.0398397149185233	4.147257550985056
>500	0.004638653528148306	1.7616686434965163
>1k	0.0024201670581643336	2.393013662209754
>5k	1.0084029409018057E-4	0.3367283098764974
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6141	0.33370320900656325	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3553	0.19307075420946412	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3444	0.18714767168516588	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3190	0.17334525919735166	No Hit
GTCCTACAGTGGACATTTCTAAATT	2985	0.16220551683513942	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2806	0.15247861984569555	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2765	0.1502506713732531	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2396	0.13019913512127104	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2312	0.12563455776309626	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	5.434020664493783E-5
10	0.0	0.0	0.0	0.0	5.434020664493783E-5
11	0.0	0.0	0.0	0.0	5.434020664493783E-5
12	0.0	0.0	0.0	0.0	5.434020664493783E-5
13	0.0	0.0	0.0	0.0	5.434020664493783E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATACT	40	0.0052834516	14.248428	4
TAGGACC	625	0.0	12.310641	4
GGTATCA	1865	0.0	12.048313	1
ACCGACT	65	8.0320716E-4	11.691018	8
TGTAGGA	1760	0.0	10.956179	2
GTAGGAC	1760	0.0	10.794265	3
GACGTGA	925	0.0	10.577213	7
AGGACGT	1760	0.0	10.308522	5
GATATAC	260	0.0	10.253633	1
CTGTAGG	1795	0.0	10.237311	1
AAATGTC	325	0.0	10.229641	7
GTATCAA	3735	0.0	10.196765	1
TAGGACG	1830	0.0	10.173741	4
ACGTGAA	1135	0.0	10.1266365	8
GTGCTAG	160	5.9499143E-9	10.116306	1
GTCCTAC	1660	0.0	10.094798	1
TCCTACA	1835	0.0	10.094255	2
TCTATAC	160	6.182745E-9	10.092637	3
GGACGTG	1795	0.0	10.054602	6
TGGCGAG	1625	0.0	10.054276	18
>>END_MODULE