FastQCFastQC Report
Thu 26 May 2016
SRR1512915_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512915_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1840258
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78100.42439701389696444No Hit
TATCAACGCAGAGTACTTTTTTTTT57430.31207580676187796No Hit
GGTATCAACGCAGAGTACTTTTTTT45350.24643283713479308No Hit
GTACTGGTTCACTATCGGTCAGTCA28540.15508694976465257No Hit
CTGTAGGACGTGGAATATGGCAAGA27860.15139181571279678No Hit
GTCCTACAGTGGACATTTCTAAATT27630.15014199095996322No Hit
CTTTAGGACGTGAAATATGGCGAGG26190.14231700120309218No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26180.14226266099644724No Hit
GTCCTAAAGTGTGTATTTCTCATTT24200.13150330008074956No Hit
GTCCTACAGTGTGCATTTCTCATTT21950.11927675358563852No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA400.00528696514.24700210
TAGGACC7150.011.97147754
GTACTAG802.8271688E-511.8912561
GGTATCA19500.011.6107441
GGCGAGG7400.011.4139619
TTAGGGT1104.897847E-711.2398874
CATTCGG700.001494248310.85485810
CCGTACA2450.010.8471917
GTCTTAC1158.6639557E-710.7538331
GTAGGAC19100.010.6573893
TAGAAAT5000.010.6519564
CCCGTAC2500.010.63053516
TAGGACT2150.010.6165664
GACGTGG7650.010.5628137
AGGACCT10800.010.4793545
TTTGGGC1002.3659217E-510.4631653
TGTAGGA19100.010.2589822
AGGACGT17200.010.2295045
CCTAGAT750.002620450710.1460992
CCATTCG750.002650199510.1323039
Produced by FastQC (version 0.11.5)