##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512915_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1840258 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30188593121182 31.0 30.0 34.0 26.0 34.0 2 30.328970720409856 31.0 30.0 34.0 26.0 34.0 3 30.50659472747843 31.0 31.0 34.0 27.0 34.0 4 34.101271126113836 37.0 35.0 37.0 30.0 37.0 5 33.68744002199691 37.0 35.0 37.0 28.0 37.0 6 33.54167730829047 37.0 35.0 37.0 28.0 37.0 7 33.38762119224587 37.0 35.0 37.0 27.0 37.0 8 33.33508399365741 37.0 35.0 37.0 27.0 37.0 9 34.66606530171313 39.0 35.0 39.0 27.0 39.0 10 34.60169660993187 39.0 35.0 39.0 27.0 39.0 11 34.57731633281855 39.0 35.0 39.0 27.0 39.0 12 34.440882745788905 39.0 35.0 39.0 26.0 39.0 13 34.430561910340835 39.0 35.0 39.0 26.0 39.0 14 35.50732505985574 40.0 36.0 41.0 26.0 41.0 15 35.50403856415785 40.0 36.0 41.0 27.0 41.0 16 35.50273602940457 40.0 36.0 41.0 27.0 41.0 17 35.49388237953591 40.0 36.0 41.0 26.0 41.0 18 35.42084044737205 40.0 36.0 41.0 26.0 41.0 19 35.43192313251729 40.0 36.0 41.0 25.0 41.0 20 35.40531327672533 40.0 36.0 41.0 25.0 41.0 21 35.37306127727742 40.0 36.0 41.0 25.0 41.0 22 35.33616590717171 40.0 36.0 41.0 25.0 41.0 23 35.17837879253887 40.0 35.0 41.0 25.0 41.0 24 35.12725661293145 40.0 35.0 41.0 25.0 41.0 25 35.06525389374751 40.0 35.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7027969051079239 1101 2 0.5528212231392011 1101 3 0.5602499709286057 1101 4 0.43247329231271436 1101 5 0.6017285447288216 1101 6 0.6228619824258175 1101 7 0.7603513988644011 1101 8 0.45822989503224676 1101 9 0.7212158529916692 1101 10 0.41675694630801985 1101 11 0.6291856170664332 1101 12 0.741061539001528 1101 13 0.9339158963701379 1101 14 0.9590492582086938 1101 15 0.6653652824681018 1101 16 0.9197932794147192 1101 17 0.860033160277446 1101 18 0.8028416781134524 1101 19 0.5641189423448054 1101 20 0.6670852425872695 1101 21 0.8475296278957387 1101 22 0.8079989045464515 1101 23 0.8461805238683198 1101 24 0.9906414875531908 1101 25 0.9234704628620065 1102 1 0.80064689905441 1102 2 0.9133714874759384 1102 3 0.569710602915368 1102 4 0.6742717910414768 1102 5 0.9088505028385114 1102 6 0.8980143291710405 1102 7 0.859956911596953 1102 8 0.9534889853910187 1102 9 0.9402652496582036 1102 10 0.931617311940208 1102 11 1.066213947507535 1102 12 1.026503849022106 1102 13 1.1361778924151764 1102 14 1.1932495891340693 1102 15 1.2702343248023382 1102 16 1.3150463162604353 1102 17 1.2198287533199377 1102 18 0.690053631783897 1102 19 0.9329251895702839 1102 20 1.0548177983804834 1102 21 1.0027840772278367 1102 22 0.7626540560860562 1102 23 1.0825016022007787 1102 24 1.0561193115170369 1102 25 1.1455657855142505 1103 1 0.8583305095387708 1103 2 1.020038021613722 1103 3 0.7441365871908161 1103 4 0.5114232814944231 1103 5 0.7826823435653054 1103 6 0.7662118700976208 1103 7 0.9140721819061781 1103 8 0.9970774036919323 1103 9 1.0187503451286943 1103 10 0.887135566137232 1103 11 0.9708018489458041 1103 12 1.0358565779574676 1103 13 0.953454026282607 1103 14 1.0450541355374057 1103 15 1.1800440298924357 1103 16 1.1092176959854996 1103 17 1.1965599946848542 1103 18 1.0353211082020408 1103 19 1.080079960113089 1103 20 0.9491566614814602 1103 21 1.1802950854081828 1103 22 1.0475253871067594 1103 23 1.2135086394844663 1103 24 1.2935128328425876 1103 25 1.1815533410521368 1104 1 0.772889595300974 1104 2 0.9279303432009591 1104 3 0.8066836501799592 1104 4 0.7053136553534216 1104 5 0.7991689245953069 1104 6 0.7451098556980824 1104 7 0.6418252372677316 1104 8 0.7697165754564566 1104 9 0.8827827032571705 1104 10 0.9983052634836653 1104 11 1.0137029401855386 1104 12 0.9729742083108306 1104 13 0.8353240647861924 1104 14 1.1967743647306577 1104 15 1.0607905290398492 1104 16 1.0995314654978259 1104 17 1.0246014308358085 1104 18 0.7057795068964055 1104 19 1.1672364109573223 1104 20 1.163821909402401 1104 21 1.1119584672640812 1104 22 1.0763043516417738 1104 23 1.0345990747736948 1104 24 1.1966761238938872 1104 25 1.0902761895620259 1105 1 0.5111330519817052 1105 2 0.6290568404698114 1105 3 0.6215029873925495 1105 4 0.3796343802017361 1105 5 0.439886352975833 1105 6 0.6921599816748767 1105 7 0.6609985629650197 1105 8 0.7062062622884184 1105 9 0.8540185725150522 1105 10 0.46789740123509915 1105 11 0.889567083354244 1105 12 0.9797706517851594 1105 13 0.9594347082493542 1105 14 0.9423794045227538 1105 15 1.0508607842891848 1105 16 0.9167597599395378 1105 17 0.7318155157436266 1105 18 0.8283888743830445 1105 19 1.0126752226152647 1105 20 1.04412468593452 1105 21 0.8110073829992075 1105 22 0.8321687109250675 1105 23 1.0731668834258414 1105 24 0.9456680394074937 1105 25 0.9262063197170392 1106 1 0.021669685899549762 1106 2 0.30900933440864975 1106 3 -1.528118680477295 1106 4 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0.9624118852275245 1107 17 0.8427169164834609 1107 18 1.015593370043213 1107 19 1.0224977629061058 1107 20 0.9348561147615797 1107 21 0.8337668756184513 1107 22 0.8776489967891834 1107 23 0.9856015604795658 1107 24 1.0086216253660822 1107 25 1.0264018403177104 1108 1 0.6141444714378039 1108 2 0.5127059981271351 1108 3 0.5329197003424326 1108 4 0.4125477742382273 1108 5 0.6851156784497476 1108 6 0.5806786444099288 1108 7 0.531716801307617 1108 8 0.4327582463273245 1108 9 0.7381169653503719 1108 10 0.662532371824625 1108 11 0.5536650463108117 1108 12 0.6902101386904249 1108 13 0.691412545980576 1108 14 0.8891142200352959 1108 15 0.8654477818342414 1108 16 0.7366778478483198 1108 17 0.688288985158529 1108 18 0.8895492216538301 1108 19 0.848015389120846 1108 20 0.837507616826521 1108 21 0.8965456559244771 1108 22 0.9151249800239469 1108 23 1.0355346793792606 1108 24 0.9819095500442003 1108 25 0.9982010367208431 1109 1 0.14325443103876978 1109 2 -0.033590472965958185 1109 3 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-3.0166748985204563 2216 20 -2.3822548864849296 2216 21 -2.9684477915970504 2216 22 -2.424122824100536 2216 23 -3.593575006706395 2216 24 -2.9245662946573248 2216 25 -3.0462307649327087 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 48401.0 3 0.0 4 765.0 5 6997.0 6 9000.0 7 7017.0 8 5619.0 9 4685.0 10 4025.0 11 3698.0 12 3411.0 13 3071.0 14 2887.0 15 2942.0 16 3101.0 17 3197.0 18 3661.0 19 4340.0 20 5756.0 21 7699.0 22 9703.0 23 11658.0 24 13657.0 25 15628.0 26 17321.0 27 19050.0 28 21431.0 29 25911.0 30 32666.0 31 41445.0 32 51925.0 33 66985.0 34 87924.0 35 114763.0 36 157573.0 37 243821.0 38 778525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.699168190436204 19.189108048222547 13.839453248647644 26.27227051269361 2 13.816978313389248 20.349110290104356 39.890436189436556 25.943475207069838 3 17.551976981370192 25.031292444539137 29.489738850079434 27.926991724011234 4 11.895845730386842 16.709733690911907 36.60697060845418 34.78744997024707 5 13.168953534000824 38.62747740941755 34.313390864632595 13.890178191949024 6 32.42190176156698 36.07020169012124 17.75562512845552 13.75227141985626 7 28.797979638682047 31.560151592355997 21.088445732427203 18.55342303653476 8 26.33994564375292 35.89806599842227 19.990355272822367 17.77163308500245 9 27.370861729829677 14.498151770588013 19.65938879344127 38.47159770614104 10 16.27179736705475 28.62918856030684 32.67651959758289 22.422494475055522 11 34.63051702944191 22.34238650208731 23.78779957895103 19.23929688951975 12 24.010107322374676 24.864882488792283 30.377557397092787 20.747452791740255 13 29.29717409981866 21.504469455595366 25.719381552769683 23.478974891816296 14 21.64290542816962 22.126643524908545 27.07726985217242 29.15318119474942 15 23.925559828437393 30.27818054942642 23.10448536652703 22.691774255609154 16 22.677776926375238 27.60968012081946 26.93884737848629 22.77369557431902 17 21.994413620332683 28.234204076752945 27.065031328286754 22.70635097462762 18 21.891020938282875 26.598901188477154 29.505263100691785 22.00481477254819 19 23.703548731547876 26.36956693031053 27.68054080049559 22.246343537646005 20 24.473385051112786 26.001878011828865 27.9919945398545 21.53274239720385 21 23.87919065564283 25.730564514157006 27.117383188742867 23.272861641457297 22 24.054992528189103 27.06295340307023 26.90292079880451 21.97913326993615 23 22.79468422362517 26.72239435992127 27.80544901856153 22.677472397892036 24 23.584519127209337 26.087266024655236 28.039492288581275 22.28872255955415 25 22.940968059913338 26.613061864151653 27.918748349416223 22.527221726518782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 95.0 1 95.0 2 323.5 3 552.0 4 552.0 5 552.0 6 1225.0 7 1898.0 8 1898.0 9 1898.0 10 2283.0 11 2668.0 12 2668.0 13 2668.0 14 3875.0 15 5082.0 16 5082.0 17 5082.0 18 10767.5 19 16453.0 20 16453.0 21 16453.0 22 27449.5 23 38446.0 24 38446.0 25 38446.0 26 58716.0 27 78986.0 28 78986.0 29 78986.0 30 101762.5 31 124539.0 32 124539.0 33 124539.0 34 143254.5 35 161970.0 36 161970.0 37 161970.0 38 175414.0 39 188858.0 40 188858.0 41 188858.0 42 204557.0 43 220256.0 44 220256.0 45 220256.0 46 237458.5 47 254661.0 48 254661.0 49 254661.0 50 246803.5 51 238946.0 52 238946.0 53 238946.0 54 213375.0 55 187804.0 56 187804.0 57 187804.0 58 161427.0 59 135050.0 60 135050.0 61 135050.0 62 113760.5 63 92471.0 64 92471.0 65 92471.0 66 71680.0 67 50889.0 68 50889.0 69 50889.0 70 37904.0 71 24919.0 72 24919.0 73 24919.0 74 17465.5 75 10012.0 76 10012.0 77 10012.0 78 6968.5 79 3925.0 80 3925.0 81 3925.0 82 2608.0 83 1291.0 84 1291.0 85 1291.0 86 842.5 87 394.0 88 394.0 89 394.0 90 240.5 91 87.0 92 87.0 93 87.0 94 46.5 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08781377393821953 2 0.04249404159634138 3 0.019399453772242805 4 0.005597041284428597 5 0.01804094860611936 6 0.05977422730943161 7 0.07493514496336927 8 0.04950392825353836 9 0.021138340384880813 10 0.047439000401030725 11 0.00499929901133428 12 4.3472165315950267E-4 13 0.0054883608711387205 14 3.2604123986962693E-4 15 0.012498247528335702 16 0.008531412443255238 17 0.003695134051855773 18 0.004781938184754529 19 0.00124982475283357 20 7.607628930291296E-4 21 1.6302061993481347E-4 22 4.3472165315950267E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1840258.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.99501449164673 #Duplication Level Percentage of deduplicated Percentage of total 1 72.2730303599351 38.301102912799855 2 15.175187727304088 16.084185870438795 3 5.511960249241556 8.76319239857811 4 2.589633093140831 5.489505731961851 5 1.3542407650608559 3.5884004484789407 6 0.8174418557502484 2.599220579493781 7 0.5280132705550676 1.9587449647393322 8 0.35091100184852764 1.4877226902592797 9 0.2381197517690396 1.1357243726172819 >10 1.0551460217381465 9.4182805237846 >50 0.05901922396303773 2.186005891969976 >100 0.03998155057987231 4.179497966007913 >500 0.004842409413222757 1.8377522728629894 >1k 0.002266659725338312 2.2306593189002486 >5k 2.060599750307556E-4 0.7400040571071392 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7810 0.42439701389696444 No Hit TATCAACGCAGAGTACTTTTTTTTT 5743 0.31207580676187796 No Hit GGTATCAACGCAGAGTACTTTTTTT 4535 0.24643283713479308 No Hit GTACTGGTTCACTATCGGTCAGTCA 2854 0.15508694976465257 No Hit CTGTAGGACGTGGAATATGGCAAGA 2786 0.15139181571279678 No Hit GTCCTACAGTGGACATTTCTAAATT 2763 0.15014199095996322 No Hit CTTTAGGACGTGAAATATGGCGAGG 2619 0.14231700120309218 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2618 0.14226266099644724 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2420 0.13150330008074956 No Hit GTCCTACAGTGTGCATTTCTCATTT 2195 0.11927675358563852 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 1.630206199348135E-4 0.0 0.0 0.0 0.0 7 1.630206199348135E-4 0.0 0.0 0.0 0.0 8 1.630206199348135E-4 0.0 0.0 0.0 0.0 9 1.630206199348135E-4 0.0 0.0 0.0 0.0 10 1.630206199348135E-4 0.0 0.0 0.0 0.0 11 1.630206199348135E-4 0.0 0.0 0.0 0.0 12 1.630206199348135E-4 0.0 0.0 0.0 0.0 13 1.630206199348135E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGGAA 40 0.005286965 14.247002 10 TAGGACC 715 0.0 11.9714775 4 GTACTAG 80 2.8271688E-5 11.891256 1 GGTATCA 1950 0.0 11.610744 1 GGCGAGG 740 0.0 11.41396 19 TTAGGGT 110 4.897847E-7 11.239887 4 CATTCGG 70 0.0014942483 10.854858 10 CCGTACA 245 0.0 10.84719 17 GTCTTAC 115 8.6639557E-7 10.753833 1 GTAGGAC 1910 0.0 10.657389 3 TAGAAAT 500 0.0 10.651956 4 CCCGTAC 250 0.0 10.630535 16 TAGGACT 215 0.0 10.616566 4 GACGTGG 765 0.0 10.562813 7 AGGACCT 1080 0.0 10.479354 5 TTTGGGC 100 2.3659217E-5 10.463165 3 TGTAGGA 1910 0.0 10.258982 2 AGGACGT 1720 0.0 10.229504 5 CCTAGAT 75 0.0026204507 10.146099 2 CCATTCG 75 0.0026501995 10.132303 9 >>END_MODULE