FastQCFastQC Report
Thu 26 May 2016
SRR1512914_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512914_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276697
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113660.4992320014477113No Hit
TATCAACGCAGAGTACTTTTTTTTT65230.28651155599537403No Hit
GGTATCAACGCAGAGTACTTTTTTT61930.2720168735672775No Hit
GTCCTACAGTGGACATTTCTAAATT52300.2297187548452868No Hit
GTCCTAAAGTGTGTATTTCTCATTT49720.2183865485833205No Hit
CTGTAGGACGTGGAATATGGCAAGA45100.1980939931839854No Hit
CTTTAGGACGTGAAATATGGCGAGG40240.17674727906260693No Hit
GTCCTACAGTGTGCATTTCTCATTT37440.16444876063876748No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30600.13440523706053112No Hit
CTGTAGGACCTGGAATATGGCGAGA27840.12228241175703224No Hit
GTACTGGTTCACTATCGGTCAGTCA24680.1084026552501277No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23620.10374678756110277No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10950.013.1854594
TATGTCG1301.9826984E-1012.42196916
GGACCGT853.951458E-612.2924816
CGTGAAC550.003072258312.08933
ACCGATC550.003072258312.08938
GGTATCA25800.012.0311131
GCCTTAC1353.583409E-1011.9901071
ACCGGAA658.034896E-411.6907518
GGCGAGG12850.011.53153919
AGGACCT18800.011.5197435
GCGACTA2650.011.47067616
CCAACGA1850.011.29579419
TGTAGGA30200.011.2600912
GTCCTAC30200.011.2556131
GTCGAGG1104.980011E-711.22577919
CCATTCG951.36352555E-510.9980529
CTGTAGG30650.010.9971631
CGACTAA2950.010.94817617
TGGCGAG29150.010.85152118
GTAGGAC29950.010.8466363
Produced by FastQC (version 0.11.5)