##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512914_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2276697
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.909751275641863	31.0	30.0	34.0	22.0	34.0
2	28.98909341032206	31.0	30.0	34.0	22.0	34.0
3	29.176047581210852	31.0	30.0	34.0	22.0	34.0
4	32.590540155321506	35.0	33.0	37.0	25.0	37.0
5	32.7884848093532	35.0	33.0	37.0	26.0	37.0
6	32.642904172140604	36.0	33.0	37.0	25.0	37.0
7	32.61495359285843	37.0	33.0	37.0	25.0	37.0
8	32.505843333566126	37.0	33.0	37.0	23.0	37.0
9	33.76683326766803	39.0	34.0	39.0	22.0	39.0
10	33.6594614918015	38.0	34.0	39.0	19.0	39.0
11	33.679129018925224	38.0	34.0	39.0	19.0	39.0
12	33.58793462634685	38.0	34.0	39.0	18.0	39.0
13	33.540687232424865	38.0	34.0	39.0	18.0	39.0
14	34.42697029951724	39.0	34.0	41.0	17.0	41.0
15	34.58004644447636	39.0	34.0	41.0	17.0	41.0
16	34.52070257921893	39.0	34.0	41.0	17.0	41.0
17	34.32472129580704	39.0	34.0	41.0	17.0	41.0
18	34.30719678551867	39.0	34.0	41.0	17.0	41.0
19	34.42848653114578	39.0	34.0	41.0	18.0	41.0
20	34.425601210876984	39.0	34.0	41.0	18.0	41.0
21	34.34400010190201	39.0	34.0	41.0	17.0	41.0
22	34.33106249975293	39.0	34.0	41.0	16.0	41.0
23	34.015168465544605	39.0	34.0	41.0	10.0	41.0
24	34.08160637976859	39.0	34.0	41.0	2.0	41.0
25	33.80944587707543	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7018499494072508
1101	2	0.3088413757852315
1101	3	0.6744931313457876
1101	4	0.8206651064313206
1101	5	0.6551772824100155
1101	6	0.723694281514554
1101	7	0.480508984718405
1101	8	0.8077943779051324
1101	9	0.9366436656519852
1101	10	0.8253534483079292
1101	11	0.8588320738102908
1101	12	0.7602521361059615
1101	13	1.200819559674855
1101	14	1.2828105466116213
1101	15	1.0985639202623432
1101	16	0.9852457033697206
1101	17	0.30928732768500566
1101	18	0.7899859646521818
1101	19	0.30967311010198273
1101	20	-0.27469482551180135
1101	21	0.8014398878343556
1101	22	0.7930309698220057
1101	23	0.2970688767421734
1101	24	0.7625654538839512
1101	25	0.38837214399884346
1102	1	0.6819336132843006
1102	2	0.6366673399018481
1102	3	0.6197369796625374
1102	4	0.7161502762827823
1102	5	0.6274329231970199
1102	6	0.5446580011100721
1102	7	0.576861554834295
1102	8	0.574857543147445
1102	9	0.8051339703184937
1102	10	0.7756299324307463
1102	11	0.5719574446717246
1102	12	0.6152448542955895
1102	13	0.6009596560434289
1102	14	0.8453034679286731
1102	15	0.7189336921933318
1102	16	0.7418076424610405
1102	17	0.92416658074238
1102	18	0.8012092934059112
1102	19	0.7243622851338074
1102	20	0.8115406908873553
1102	21	0.9310294904186307
1102	22	0.8767491680694519
1102	23	0.817763175509377
1102	24	0.3539715634644267
1102	25	0.6136539217454313
1103	1	0.7785517875594721
1103	2	0.799128077426893
1103	3	0.929028644230538
1103	4	1.0721691663800357
1103	5	0.9218281685742653
1103	6	0.44953261303036385
1103	7	0.40838042213182746
1103	8	0.6307831927832623
1103	9	0.721105642623499
1103	10	0.9173976803807875
1103	11	0.6432234376386745
1103	12	0.8711927522946681
1103	13	0.9879608449077395
1103	14	1.0191453683472034
1103	15	0.9464048773590221
1103	16	0.9270617014834315
1103	17	0.6307714685870849
1103	18	1.0454351502063304
1103	19	1.0317486821298445
1103	20	1.1812060385474652
1103	21	0.9382801768693056
1103	22	0.9735150935313257
1103	23	0.38959573429765726
1103	24	0.5674472167809839
1103	25	0.8617654186802852
1104	1	0.7761991839938744
1104	2	0.6973179795808235
1104	3	0.759806147018594
1104	4	0.8843938354975904
1104	5	0.6595764499212038
1104	6	0.5840682361948879
1104	7	0.7302811469534731
1104	8	0.4663389320357396
1104	9	0.7637299153087085
1104	10	0.7087353718419251
1104	11	0.5219132575702545
1104	12	0.6793641095576959
1104	13	0.7389261853185687
1104	14	0.9453334519015897
1104	15	0.7095666312279576
1104	16	0.6282558230118909
1104	17	1.0576282890267095
1104	18	0.8168667957800437
1104	19	0.48084936964990277
1104	20	0.5264871816234731
1104	21	0.5883020636060294
1104	22	0.4376698256997358
1104	23	0.7239504977076194
1104	24	0.7486716031948504
1104	25	0.10148483012710585
1105	1	0.1482338501407341
1105	2	0.21313809439988418
1105	3	0.3950913976028865
1105	4	0.4627874250197692
1105	5	0.12865849827441878
1105	6	0.3177472710109157
1105	7	0.19578620645940248
1105	8	0.26508423239738477
1105	9	0.018628331775147444
1105	10	0.11687006086715712
1105	11	0.20067330064175337
1105	12	0.3100741942265657
1105	13	0.2597590539765875
1105	14	0.40439653133051223
1105	15	0.3271173289093383
1105	16	0.2018322400814938
1105	17	0.5807678120071955
1105	18	0.3225997206786104
1105	19	0.04376972523314038
1105	20	0.30321722503861537
1105	21	0.21801389855393438
1105	22	0.11823165866059071
1105	23	0.4258673914567268
1105	24	0.43104459825859465
1105	25	0.6832574295671989
1106	1	0.4683024002010434
1106	2	0.44763338859339896
1106	3	0.3564177807259057
1106	4	0.48774998853794926
1106	5	0.314904558360368
1106	6	0.37699274878878697
1106	7	0.432750787415884
1106	8	0.5236089393597112
1106	9	0.6067911070038576
1106	10	0.44661843222307596
1106	11	0.14907428889198115
1106	12	0.4561714916669928
1106	13	0.47779620491993313
1106	14	0.7714816431431117
1106	15	0.5595595575732233
1106	16	0.716754656398976
1106	17	0.8432369515010336
1106	18	0.739281132852696
1106	19	0.7379329513433888
1106	20	0.8232797802041105
1106	21	0.842360624229407
1106	22	0.7556462881229109
1106	23	0.9703511845263435
1106	24	0.5548954806795976
1106	25	0.7946422034120246
1107	1	0.5052290382752993
1107	2	0.525975899899251
1107	3	0.464633133335159
1107	4	0.5218287108875117
1107	5	0.42635964379348223
1107	6	0.5470709902422115
1107	7	0.2467010442858566
1107	8	0.375377931490263
1107	9	0.2694023904651246
1107	10	0.5234566185128315
1107	11	0.45610077389839176
1107	12	0.4832624097865832
1107	13	0.3526085726626036
1107	14	0.6200888738347388
1107	15	0.3510701725714327
1107	16	0.499437734517592
1107	17	0.7301858484455153
1107	18	0.7194814104134011
1107	19	0.48404592534625124
1107	20	0.632992139844724
1107	21	0.43533344227207493
1107	22	0.5867598327543391
1107	23	0.8811135878570724
1107	24	0.6660454322850669
1107	25	0.7918459056153466
1108	1	0.7291810274957022
1108	2	0.640423871781092
1108	3	0.6092875706828131
1108	4	0.7189328682803335
1108	5	0.5866422728455802
1108	6	0.6475997425828481
1108	7	0.6718851868572955
1108	8	0.5744757383780197
1108	9	0.6529370168692665
1108	10	0.7544714697973944
1108	11	0.5162979787931334
1108	12	0.7428899526362827
1108	13	0.6329706850228973
1108	14	0.746558891688025
1108	15	0.7407510349614128
1108	16	0.47228971655643903
1108	17	0.7268352486460898
1108	18	0.8899483622743602
1108	19	0.7855222235656996
1108	20	0.8149061942563094
1108	21	0.6947208082711356
1108	22	0.8150221511441771
1108	23	1.1001652148421641
1108	24	0.9826361756820177
1108	25	1.1162254208074032
1109	1	0.6124861180644494
1109	2	0.6233753253454672
1109	3	0.6674015577689758
1109	4	0.6145614815743272
1109	5	0.5596125464014321
1109	6	0.6904895767423795
1109	7	0.5920965766241366
1109	8	0.6357234292266085
1109	9	0.7416608778628486
1109	10	0.7964862571075599
1109	11	0.6612352706847062
1109	12	0.6137423376852738
1109	13	0.7501468594739791
1109	14	0.7265414337403229
1109	15	0.7019244761539056
1109	16	0.41172748982049967
1109	17	0.7840969732027219
1109	18	0.9505763626128285
1109	19	0.8511309621978072
1109	20	0.6896936749460565
1109	21	0.7097292266474184
1109	22	0.7619194963911795
1109	23	0.8735161871558503
1109	24	0.7253635175178204
1109	25	0.36257242442216153
1110	1	0.5615418221310406
1110	2	0.5355593171308293
1110	3	0.5795379367895279
1110	4	0.5241764635331023
1110	5	0.45391098952138265
1110	6	0.5432421538549761
1110	7	0.42949547120488774
1110	8	0.5119634405848359
1110	9	0.669678695362542
1110	10	0.574932948020404
1110	11	0.5086823229956465
1110	12	0.522883916822849
1110	13	0.5591025767344675
1110	14	0.5505889633262058
1110	15	0.4970304719203824
1110	16	0.5086427706497645
1110	17	0.6283775615358778
1110	18	0.06237550845129647
1110	19	0.380828707188428
1110	20	0.43893968665994976
1110	21	0.48295464727464577
1110	22	0.572247952762396
1110	23	0.7749045581682807
1110	24	0.5360607576984009
1110	25	0.7309816417945072
1111	1	0.4471712642774399
1111	2	0.4154562479076205
1111	3	0.5501677788719377
1111	4	0.300110885454167
1111	5	0.46448729731213945
1111	6	0.26709519451133446
1111	7	0.2621082531597736
1111	8	0.47388467604430673
1111	9	0.4274625927960969
1111	10	0.47014200028633013
1111	11	0.13196574050598286
1111	12	-0.11341913183222374
1111	13	0.23133987590945537
1111	14	0.315567995252529
1111	15	0.2598001430587118
1111	16	0.3807046693174101
1111	17	-0.09690582169423578
1111	18	0.23184924979554467
1111	19	0.16618305049350113
1111	20	0.30705364556937553
1111	21	0.30666228262442274
1111	22	-0.03240073201946814
1111	23	0.25598130017527154
1111	24	0.3554593682064393
1111	25	0.49680653838248645
1112	1	0.26697969951277045
1112	2	0.22737006198354592
1112	3	0.2204819210239961
1112	4	0.30851104000625185
1112	5	0.20328096969989673
1112	6	0.05795316681342655
1112	7	-0.059929676883427874
1112	8	0.14750878940872525
1112	9	0.07094876032662256
1112	10	0.13634391171155613
1112	11	0.011172469537193308
1112	12	0.16310965841017122
1112	13	0.27464540490618816
1112	14	0.24507804431934233
1112	15	-0.0018514183395907935
1112	16	0.11388787542869494
1112	17	0.34011434816267183
1112	18	0.01604577850480382
1112	19	0.14140685313419254
1112	20	-0.07039889994164383
1112	21	0.11046326905078985
1112	22	-0.06818717481697689
1112	23	0.06713493431832518
1112	24	0.06340668283503703
1112	25	0.10210642553401073
1113	1	-0.11610955881037555
1113	2	0.04096822578260628
1113	3	0.10421034335379886
1113	4	0.10366289918660954
1113	5	-0.12065165331171102
1113	6	-0.3968563013330737
1113	7	-0.38951299396302375
1113	8	-0.11008327285136943
1113	9	-0.2702941995654413
1113	10	-0.28552989027628684
1113	11	-0.32148336340044636
1113	12	-0.47791041237091036
1113	13	-0.19588063760819097
1113	14	0.04912999886987279
1113	15	-0.2910086768976825
1113	16	-0.40494246564858827
1113	17	-0.15401816661029244
1113	18	-0.34724553724938545
1113	19	-0.857332754005256
1113	20	-0.5077370632116143
1113	21	-0.2366810286691603
1113	22	-0.8102861795505092
1113	23	-4.408282472733866
1113	24	-1.9392596018128785
1113	25	-1.0579495680129227
1114	1	-0.44907800755645155
1114	2	-0.6861464885807464
1114	3	-0.45698044411951244
1114	4	-0.5451972857733658
1114	5	-0.540066278247032
1114	6	-1.0597531987081226
1114	7	-0.9666406260702338
1114	8	-0.7221532191205462
1114	9	-0.921430704865621
1114	10	-0.6571600038345196
1114	11	-0.8368813384739582
1114	12	-1.6917172483092173
1114	13	-0.913804440240682
1114	14	-2.6124044567010998
1114	15	-1.4475740907342995
1114	16	-1.6227762872701987
1114	17	-1.3412686909031706
1114	18	-1.3924889490983716
1114	19	-1.3319372502583775
1114	20	-0.9836018628597216
1114	21	-0.7679481898125857
1114	22	-1.1337858447951277
1114	23	-5.234635467141704
1114	24	-2.421822274013884
1114	25	-2.4109562552325023
1115	1	-1.5542717460587063
1115	2	-1.4592895905014736
1115	3	-1.2109129621545698
1115	4	-1.2332683631783787
1115	5	-1.1667017035058507
1115	6	-1.3444409643870863
1115	7	-1.909457583936689
1115	8	-2.1895447085732975
1115	9	-3.286548910306987
1115	10	-4.0651465617617575
1115	11	-2.7231481459773086
1115	12	-2.8741850022246176
1115	13	-2.2857166039104904
1115	14	-3.0707831551378213
1115	15	-2.657444826934931
1115	16	-2.710572357612275
1115	17	-2.6923369959680095
1115	18	-5.2992184129743265
1115	19	-3.229999843402936
1115	20	-3.450439058940457
1115	21	-3.11533704185241
1115	22	-2.6076348039995025
1115	23	-3.249250402671368
1115	24	-2.928483269951659
1115	25	-3.1225998656412024
1116	1	-1.0584767104409387
1116	2	-0.8358153786203282
1116	3	-1.3011118369326269
1116	4	-0.6976099514669407
1116	5	-0.9248745370257723
1116	6	-3.031674900738601
1116	7	-1.6039049202696667
1116	8	-1.936426816973956
1116	9	-3.1391590972022136
1116	10	-2.281475828228288
1116	11	-1.8533473059827266
1116	12	-1.7737150901459522
1116	13	-3.1139255899985265
1116	14	-6.209205419985686
1116	15	-3.881252754829454
1116	16	-2.7767351226063184
1116	17	-3.756436140998993
1116	18	-2.2983413388366074
1116	19	-2.2110151101974793
1116	20	-2.5902375458093054
1116	21	-3.4277209008360003
1116	22	-3.051668204123576
1116	23	-1.8999538446525577
1116	24	-3.241071066498982
1116	25	-3.2644206145056387
1201	1	0.5786401931877876
1201	2	0.4784326904008154
1201	3	0.8644274224329891
1201	4	0.8336915430621232
1201	5	0.3613994806831897
1201	6	0.7389768751345258
1201	7	0.8694920037985057
1201	8	0.923314770003195
1201	9	1.3808053811122392
1201	10	1.2410877418941624
1201	11	1.4220484368446122
1201	12	1.282955994610795
1201	13	1.3240880431375075
1201	14	1.4137049812681894
1201	15	1.4073703515202283
1201	16	1.385615872429213
1201	17	1.6146075317309325
1201	18	1.2999824816268557
1201	19	1.4042624723491102
1201	20	1.3675476336602728
1201	21	1.5930064370465402
1201	22	1.3015412658323058
1201	23	1.7431748388068726
1201	24	1.6274328177804378
1201	25	1.563324428113745
1202	1	0.7020230569094821
1202	2	0.6652306191323376
1202	3	0.7697748236200006
1202	4	0.9296484120674506
1202	5	0.7294407872681603
1202	6	0.9241900954042066
1202	7	1.024228520889217
1202	8	1.042263389189273
1202	9	1.3100735651507165
1202	10	1.0883738372804856
1202	11	1.1074605832557793
1202	12	1.2213670906157859
1202	13	1.2819513365244433
1202	14	1.6823598179526797
1202	15	1.4537423384554415
1202	16	1.2001237367695765
1202	17	1.5585574168106362
1202	18	1.5564437740261994
1202	19	1.571439087924439
1202	20	1.268553533676375
1202	21	1.4354206123139832
1202	22	1.396619890199979
1202	23	1.5670324221569274
1202	24	1.5555313541789744
1202	25	1.990871491754504
1203	1	0.9185453682303297
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2211	14	0.13402070410115385
2211	15	-0.3317621239184163
2211	16	-0.3068668058771493
2211	17	0.15736405557407807
2211	18	-0.23781809562872525
2211	19	0.0034235459815974423
2211	20	-0.251776508729435
2211	21	-0.02955822044810219
2211	22	0.04455639281204071
2211	23	0.3113816845043189
2211	24	0.01055604884717809
2211	25	0.3224345326938689
2212	1	0.06572166588012252
2212	2	0.17168414216845918
2212	3	-0.16671215837828868
2212	4	0.03878508954407778
2212	5	-0.16113745821830605
2212	6	-0.11879986932497388
2212	7	-0.046629632015587674
2212	8	-0.06411598208325842
2212	9	0.008871730835252833
2212	10	-0.08656251906324286
2212	11	-0.3274062650653349
2212	12	0.09843171742138423
2212	13	-0.06642662953665734
2212	14	-0.029484872258940698
2212	15	-0.30481380717608175
2212	16	-0.36408308454288374
2212	17	-0.2268410023378351
2212	18	0.03807103878494189
2212	19	-0.09439252138417231
2212	20	-0.07916013413173317
2212	21	0.10720823876166463
2212	22	0.13055310178055635
2212	23	0.10700905536009486
2212	24	0.10722886213117278
2212	25	0.31402712908301567
2213	1	-0.26725125882439116
2213	2	-0.1107724269807484
2213	3	-0.3355525539716382
2213	4	-0.10053148731962125
2213	5	-0.20648151267268844
2213	6	0.03023692365502484
2213	7	-0.17793206502699377
2213	8	-0.020200213676410783
2213	9	-0.4233334728577631
2213	10	-0.10267711151611536
2213	11	-0.28183482636747925
2213	12	-0.17340246330950748
2213	13	-0.24971374955411108
2213	14	-0.3537953318420861
2213	15	-0.2540585600775316
2213	16	-0.20781901686891047
2213	17	-0.1565870960698561
2213	18	-0.19031341917980882
2213	19	-0.24191495371434257
2213	20	0.007589026065531357
2213	21	-0.24871415416750864
2213	22	-0.419688229310232
2213	23	-0.2816177739553467
2213	24	-0.10270617225724266
2213	25	0.09584205119701039
2214	1	-2.556927704453809
2214	2	-1.3899722864283461
2214	3	-1.582701865450833
2214	4	-3.4240085345054894
2214	5	-1.7521627562678148
2214	6	-1.5361919490970948
2214	7	-1.353022088057621
2214	8	-1.0914104129260842
2214	9	-0.9937068176232131
2214	10	-1.0462733639740662
2214	11	-1.0490774544624983
2214	12	-1.0192846987974207
2214	13	-1.3841451240606801
2214	14	-1.3044642281631553
2214	15	-1.5889439893153678
2214	16	-1.5250891486025324
2214	17	-3.704587307485724
2214	18	-3.4306232804577235
2214	19	-2.251749951458258
2214	20	-1.9790053970578825
2214	21	-1.8524750565217616
2214	22	-2.8023343142504764
2214	23	-1.77829580422501
2214	24	-2.941054759832838
2214	25	-2.8839449766775864
2215	1	-1.8665753637368958
2215	2	-3.5276681291079406
2215	3	-2.1012317809363275
2215	4	-3.6074780117686984
2215	5	-2.255169625417178
2215	6	-2.0089094822462314
2215	7	-2.2083987567034704
2215	8	-1.5026430120286847
2215	9	-3.9837403731770884
2215	10	-2.3656382630553274
2215	11	-1.8270736418577727
2215	12	-1.7560214083400005
2215	13	-1.8522145812795507
2215	14	-1.5511170100732983
2215	15	-1.7877132562000497
2215	16	-1.8225264767246827
2215	17	-2.17495620437726
2215	18	-1.804876591670876
2215	19	-1.9269982440102353
2215	20	-1.7783592586893988
2215	21	-2.3371930734518322
2215	22	-2.124981452746347
2215	23	-2.0269782717898757
2215	24	-2.0642423119300624
2215	25	-2.8921725612985405
2216	1	-2.4325329059532024
2216	2	-2.3057090453079603
2216	3	-3.8275188730211056
2216	4	-4.527445822725941
2216	5	-3.9438492262778944
2216	6	-2.8553153926198647
2216	7	-4.240132838367412
2216	8	-2.3820051211866264
2216	9	-2.9370271156180863
2216	10	-1.9804658256503487
2216	11	-2.1329083300355123
2216	12	-2.650631379792827
2216	13	-2.1908264651687297
2216	14	-3.1606760407904453
2216	15	-2.6668832733671337
2216	16	-2.8866745366258755
2216	17	-2.5255451753883342
2216	18	-1.8961345881817095
2216	19	-2.7930370465923886
2216	20	-3.33680618585381
2216	21	-2.8714599213434546
2216	22	-2.6376498892447984
2216	23	-2.633565996929871
2216	24	-2.8466417166266815
2216	25	-2.796296600611349
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112425.0
3	2.0
4	998.0
5	6402.0
6	8316.0
7	7655.0
8	6807.0
9	6114.0
10	5324.0
11	4924.0
12	4607.0
13	4365.0
14	4460.0
15	4376.0
16	4469.0
17	4858.0
18	5438.0
19	6719.0
20	8649.0
21	11267.0
22	14187.0
23	16907.0
24	20091.0
25	22507.0
26	24612.0
27	27237.0
28	30340.0
29	35481.0
30	43050.0
31	53717.0
32	66448.0
33	85213.0
34	110178.0
35	145189.0
36	198737.0
37	309994.0
38	854634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.92085844226419	19.210761644057794	12.896819537873736	23.971560375804284
2	14.155719903429517	20.62612674348729	42.978037946436416	22.240115406646776
3	19.019719210669308	26.565872073537093	29.332869465598893	25.081539250194705
4	11.82309894445716	16.95435762295741	39.25328767177445	31.969255760810977
5	12.204116506219128	39.608029185857774	35.44504699213027	12.742807315792826
6	30.21520369533529	37.763773546421156	18.91859219963532	13.102430558608235
7	27.378402804885337	32.39891370476261	22.379255594121556	17.843427896230494
8	24.652656099749773	37.74032891430491	20.980015661748443	16.626999324196873
9	27.304246527167226	15.056708282046774	20.542421311763672	37.09662387902233
10	15.576489704332014	29.462474600443322	33.981991043881116	20.97904465134355
11	33.54302670623145	23.057259039454888	25.264007033739972	18.13570722057369
12	23.69586732094047	25.449849877840265	31.93774755647552	18.916535244743745
13	28.90441363624175	22.953625190182304	26.252785420726564	21.889175752849383
14	20.613536470958547	23.4953932670217	28.174838683016855	27.716231579002898
15	22.600329706561162	32.36984283987251	23.831761732058467	21.19806572150786
16	20.874530548474127	28.824796272435087	29.22233813346557	21.078335045625217
17	20.564716992994786	29.545881222221293	28.533636058667394	21.355765726116523
18	20.373593035525854	27.86370031656701	31.566830812521985	20.19587583538516
19	22.84267463193673	27.136595910999183	28.780136300027127	21.240593157036958
20	23.34657241914764	27.51068549858479	29.377493319639953	19.765248762627614
21	22.525560061859352	27.140789805691657	28.09914376320424	22.234506369244755
22	22.728046166529268	28.795339378950263	28.20972794723825	20.26688650728222
23	21.51243487118883	28.452546920661888	29.14666803837802	20.888350169771265
24	22.45708223847192	27.180772702153877	29.803252469290758	20.558892590083445
25	21.79885266419919	27.967464162791728	29.47186415909887	20.761819013910205
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	418.0
1	418.0
2	977.5
3	1537.0
4	1537.0
5	1537.0
6	2906.0
7	4275.0
8	4275.0
9	4275.0
10	5325.5
11	6376.0
12	6376.0
13	6376.0
14	9040.5
15	11705.0
16	11705.0
17	11705.0
18	21330.0
19	30955.0
20	30955.0
21	30955.0
22	48932.5
23	66910.0
24	66910.0
25	66910.0
26	97423.0
27	127936.0
28	127936.0
29	127936.0
30	164740.0
31	201544.0
32	201544.0
33	201544.0
34	221462.0
35	241380.0
36	241380.0
37	241380.0
38	252849.0
39	264318.0
40	264318.0
41	264318.0
42	272504.0
43	280690.0
44	280690.0
45	280690.0
46	289581.5
47	298473.0
48	298473.0
49	298473.0
50	279794.0
51	261115.0
52	261115.0
53	261115.0
54	224745.0
55	188375.0
56	188375.0
57	188375.0
58	159565.0
59	130755.0
60	130755.0
61	130755.0
62	107294.5
63	83834.0
64	83834.0
65	83834.0
66	63679.5
67	43525.0
68	43525.0
69	43525.0
70	31870.5
71	20216.0
72	20216.0
73	20216.0
74	14181.0
75	8146.0
76	8146.0
77	8146.0
78	5551.5
79	2957.0
80	2957.0
81	2957.0
82	1937.0
83	917.0
84	917.0
85	917.0
86	591.5
87	266.0
88	266.0
89	266.0
90	156.5
91	47.0
92	47.0
93	47.0
94	33.5
95	20.0
96	20.0
97	20.0
98	13.5
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34040542065984186
2	0.009882738019156699
3	0.008169730095836205
4	0.010453740660263531
5	0.011068666581455503
6	0.08033567927572267
7	0.07945721367401987
8	0.10392248068144334
9	0.08332246232151226
10	0.09008664745462396
11	0.0855186263257693
12	0.0973339886686722
13	0.08371777184227852
14	0.08709986440883437
15	0.0855186263257693
16	0.08644101520755726
17	0.09873953363139672
18	0.10089177435556862
19	0.09469859186356375
20	0.09307343050041353
21	0.0816533776782769
22	0.10198985635769713
23	0.09513782466441516
24	0.09030626385504965
25	0.08942779825334685
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2276697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.150949833855556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26664977936224	41.510272261439816
2	14.008431174555966	15.451565699179087
3	4.768373356946505	7.889409593941503
4	2.1690927316778414	4.785100977189813
5	1.1502329232011406	3.1718219122357567
6	0.7106973735946877	2.3517381118904104
7	0.42898291742171346	1.6561170750814143
8	0.28370850970706035	1.2517435029033606
9	0.2039845591608728	1.0124947970246192
>10	0.9045140205810471	8.50839489237672
>50	0.05857129501465654	2.2179937186448595
>100	0.038731535558466895	4.162318070415958
>500	0.004978490395040517	1.9261421305245712
>1k	0.002730139894054298	2.8067983683414113
>5k	2.4089469653420275E-4	0.7947428765896966
>10k+	8.02982321780676E-5	0.5033460122210237
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11366	0.4992320014477113	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6523	0.28651155599537403	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6193	0.2720168735672775	No Hit
GTCCTACAGTGGACATTTCTAAATT	5230	0.2297187548452868	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4972	0.2183865485833205	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4510	0.1980939931839854	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4024	0.17674727906260693	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3744	0.16444876063876748	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3060	0.13440523706053112	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2784	0.12228241175703224	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2468	0.1084026552501277	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2362	0.10374678756110277	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	8.784656017028177E-5	0.0
10	0.0	0.0	0.0	1.3176984025542265E-4	0.0
11	0.0	0.0	0.0	1.7569312034056354E-4	0.0
12	4.3923280085140884E-5	0.0	0.0	1.7569312034056354E-4	0.0
13	4.3923280085140884E-5	0.0	0.0	1.7569312034056354E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1095	0.0	13.185459	4
TATGTCG	130	1.9826984E-10	12.421969	16
GGACCGT	85	3.951458E-6	12.292481	6
CGTGAAC	55	0.0030722583	12.0893	3
ACCGATC	55	0.0030722583	12.0893	8
GGTATCA	2580	0.0	12.031113	1
GCCTTAC	135	3.583409E-10	11.990107	1
ACCGGAA	65	8.034896E-4	11.690751	8
GGCGAGG	1285	0.0	11.531539	19
AGGACCT	1880	0.0	11.519743	5
GCGACTA	265	0.0	11.470676	16
CCAACGA	185	0.0	11.295794	19
TGTAGGA	3020	0.0	11.260091	2
GTCCTAC	3020	0.0	11.255613	1
GTCGAGG	110	4.980011E-7	11.225779	19
CCATTCG	95	1.36352555E-5	10.998052	9
CTGTAGG	3065	0.0	10.997163	1
CGACTAA	295	0.0	10.948176	17
TGGCGAG	2915	0.0	10.851521	18
GTAGGAC	2995	0.0	10.846636	3
>>END_MODULE