FastQCFastQC Report
Thu 26 May 2016
SRR1512914_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512914_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2276697
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145680.6398743442803324No Hit
TATCAACGCAGAGTACTTTTTTTTT108170.4751181206809689No Hit
GGTATCAACGCAGAGTACTTTTTTT84300.3702732511177377No Hit
ACGCAGAGTACTTTTTTTTTTTTTT52800.23191491884954388No Hit
GTCCTACAGTGGACATTTCTAAATT47260.2075814216823758No Hit
CTGTAGGACGTGGAATATGGCAAGA44540.19563428949921752No Hit
GTCCTAAAGTGTGTATTTCTCATTT44290.194536207497089No Hit
CTTTAGGACGTGAAATATGGCGAGG41150.18074429755035476No Hit
GTCCTACAGTGTGCATTTCTCATTT34780.15276516813612No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30020.13185768681559296No Hit
CTGTAGGACCTGGAATATGGCGAGA27370.12021801759303061No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25930.11389306526077031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10600.013.8163374
CCATCGC1102.7866918E-912.9427619
TCCAACG2500.012.90909818
GGTATCA25100.012.2005481
TCGAGAC550.00304373112.1043683
GTCTTAC1650.012.10411
GGACCGG802.8371813E-511.8874336
AGGACCT18550.011.6375085
CCCGTAC1800.011.60207716
GGCGAGG11900.011.32582219
GGACCTG17900.011.2100486
CCACGAC803.733542E-410.6993963
GTATCAA52500.010.6334931
TGTAGGA31750.010.5440072
GTAGGAC29800.010.4999293
CGTGCGC1909.094947E-1210.49713310
GTGCGCC1909.094947E-1210.493443511
CCAACGA2100.010.39529619
GCGCCAC1856.002665E-1110.26318113
CTGTAGG31400.010.2069841
Produced by FastQC (version 0.11.5)