##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512914_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2276697
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.246361724902346	31.0	30.0	34.0	26.0	34.0
2	30.277946516378773	31.0	30.0	34.0	26.0	34.0
3	30.43817644596536	31.0	30.0	34.0	26.0	34.0
4	34.00006368875612	37.0	35.0	37.0	30.0	37.0
5	33.62625856668674	37.0	35.0	37.0	28.0	37.0
6	33.491963577059224	37.0	35.0	37.0	28.0	37.0
7	33.319636297671586	37.0	35.0	37.0	27.0	37.0
8	33.257041231222246	37.0	35.0	37.0	27.0	37.0
9	34.568180131128564	39.0	35.0	39.0	27.0	39.0
10	34.48184628872441	39.0	35.0	39.0	27.0	39.0
11	34.46332208458131	39.0	35.0	39.0	27.0	39.0
12	34.34608206537805	39.0	35.0	39.0	26.0	39.0
13	34.32973645592716	39.0	35.0	39.0	25.0	39.0
14	35.3988356816915	40.0	36.0	41.0	25.0	41.0
15	35.40338525504272	40.0	36.0	41.0	26.0	41.0
16	35.400647956227814	40.0	36.0	41.0	26.0	41.0
17	35.41271719512961	40.0	36.0	41.0	26.0	41.0
18	35.343883705209784	40.0	36.0	41.0	25.0	41.0
19	35.373314059798034	40.0	36.0	41.0	25.0	41.0
20	35.35590023617548	40.0	36.0	41.0	25.0	41.0
21	35.323587635948044	40.0	36.0	41.0	25.0	41.0
22	35.288101578734455	40.0	36.0	41.0	25.0	41.0
23	35.14020618466137	40.0	35.0	41.0	25.0	41.0
24	35.104970050911476	40.0	35.0	41.0	24.0	41.0
25	35.03775469462998	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8634888505815574
1101	2	0.7156437596363645
1101	3	0.7244790699352457
1101	4	0.7475415471364499
1101	5	0.7280720389119253
1101	6	0.8593886727525799
1101	7	0.9479854204067593
1101	8	0.6607970253556061
1101	9	1.033777856526875
1101	10	0.7698979736434737
1101	11	1.021924254074726
1101	12	1.0709852078948074
1101	13	1.1827054275899656
1101	14	1.2778287513888884
1101	15	0.9764347390167316
1101	16	1.1693976328546398
1101	17	1.228267052309981
1101	18	1.0899772288750995
1101	19	1.049901703009084
1101	20	1.0758596169509929
1101	21	1.19365159035938
1101	22	1.0805071533016672
1101	23	1.2720131009155224
1101	24	1.20717654229842
1101	25	1.3684428685635979
1102	1	0.874267607269374
1102	2	0.9845716192396132
1102	3	0.6584886637050218
1102	4	0.7226822898195593
1102	5	0.880700292336158
1102	6	0.7833713121287715
1102	7	0.7789100779831628
1102	8	0.8592042755273397
1102	9	0.9582800096775657
1102	10	0.9032128870127067
1102	11	0.9186671212244661
1102	12	1.0151094701729377
1102	13	1.1031198721657205
1102	14	1.0788555912924735
1102	15	1.145674968587656
1102	16	1.0920987228487036
1102	17	1.2229755059809193
1102	18	0.647824995335192
1102	19	0.8798431161357954
1102	20	1.104266910210363
1102	21	1.0144157988149018
1102	22	0.7498330195478005
1102	23	1.079565486247013
1102	24	1.041604686280067
1102	25	1.0365502780364722
1103	1	0.9919577979913434
1103	2	1.1226133996689818
1103	3	0.843275551804247
1103	4	0.7698706510495299
1103	5	0.9871099072055216
1103	6	0.8993704484213794
1103	7	1.0547970098042256
1103	8	1.1162637720914361
1103	9	1.0930818442659742
1103	10	1.1508555738281743
1103	11	1.2211469568756783
1103	12	1.367578829870105
1103	13	1.2749209732883315
1103	14	1.360977876522746
1103	15	1.3134031141530471
1103	16	1.366818472451051
1103	17	1.2882173038155713
1103	18	1.3752561692112977
1103	19	1.369206967925038
1103	20	1.2910531706464567
1103	21	1.457780973286475
1103	22	1.401630860160111
1103	23	1.4348988942879544
1103	24	1.4278932718414907
1103	25	1.4167112500857968
1104	1	0.9927823013698145
1104	2	1.065535202535969
1104	3	0.936051193042978
1104	4	0.8911375136181761
1104	5	1.0736656261598156
1104	6	1.0237055726036104
1104	7	0.8932210441475732
1104	8	1.0629906017887976
1104	9	1.1012752500628409
1104	10	1.0963298732393199
1104	11	1.177001497152716
1104	12	1.221630626715303
1104	13	1.129795802629154
1104	14	1.3305589161368587
1104	15	1.2724288042986274
1104	16	1.3190344898357083
1104	17	1.2928352875940874
1104	18	0.8955977435089721
1104	19	1.3617948950951515
1104	20	1.3175671577126877
1104	21	1.3903905636191283
1104	22	1.1960799101021706
1104	23	1.2966149468267147
1104	24	1.4293849676782102
1104	25	1.302073803585479
1105	1	0.5745438575815562
1105	2	0.6077936778686279
1105	3	0.5886771456031568
1105	4	0.38078125398577356
1105	5	0.43250821155103125
1105	6	0.540955204271981
1105	7	0.504124081623857
1105	8	0.44023454764762704
1105	9	0.7923006871948672
1105	10	0.4397386751718315
1105	11	0.7543730794824128
1105	12	0.7719491330372961
1105	13	0.7858558359433729
1105	14	0.8437215361747548
1105	15	0.9076897888416724
1105	16	0.8168582836481733
1105	17	0.6317666416515451
1105	18	0.6562562570279553
1105	19	0.8682405441394678
1105	20	0.92376381828403
1105	21	0.7689487263049131
1105	22	0.7643137295385571
1105	23	0.9659586034474117
1105	24	0.8981732806877645
1105	25	0.8126296405092575
1106	1	0.24165753811747948
1106	2	0.48913403222046625
1106	3	-1.4404200358814236
1106	4	-0.11383624532046355
1106	5	0.30146196778031253
1106	6	0.42335954088666483
1106	7	0.5762581369924433
1106	8	0.6273474873752036
1106	9	0.7853928725370807
1106	10	0.4647082302546863
1106	11	0.6641993740580077
1106	12	0.9358622017071809
1106	13	0.7609392382440348
1106	14	0.7992111528458565
1106	15	0.8805358160672725
1106	16	0.8151176071785855
1106	17	0.790723434748756
1106	18	0.8750714054875033
1106	19	0.8650070942329506
1106	20	1.0324937313885485
1106	21	0.8791857678674617
1106	22	0.7870176457742559
1106	23	0.8814682219437628
1106	24	1.0461323425477218
1106	25	1.1006713947285363
1107	1	0.6371401635664675
1107	2	0.7958784698986783
1107	3	0.6458047726095302
1107	4	0.4999518833401666
1107	5	0.5971980655695006
1107	6	0.6126533727838464
1107	7	0.6228236063845358
1107	8	0.5969266684055938
1107	9	0.650145407042686
1107	10	0.6188700727141523
1107	11	0.7624258953058671
1107	12	0.813081010321838
1107	13	0.7265026751529859
1107	14	0.8677199043715262
1107	15	0.6990950965351317
1107	16	0.725743016092764
1107	17	0.6852229237966085
1107	18	0.7150216344794487
1107	19	0.718085265120088
1107	20	0.7934870432732168
1107	21	0.7865037090395788
1107	22	0.8141863174816208
1107	23	0.903102885495052
1107	24	0.9552466747336936
1107	25	0.8848507393681544
1108	1	0.5849745619772477
1108	2	0.4918227505459569
1108	3	0.5879782487579703
1108	4	0.45100303080987203
1108	5	0.4954665427700249
1108	6	0.5820668718126569
1108	7	0.39582742099091917
1108	8	0.40631504038099564
1108	9	0.6500190312652592
1108	10	0.6707964056258149
1108	11	0.5410602433060561
1108	12	0.4988308385374083
1108	13	0.6678970785154021
1108	14	0.7720009065363271
1108	15	0.6807265274833796
1108	16	0.584669435350726
1108	17	0.6831851231179655
1108	18	0.7711493157906091
1108	19	0.7425297216735061
1108	20	0.7537111580349958
1108	21	0.759548223771958
1108	22	0.7413771732684893
1108	23	0.7866179512481821
1108	24	0.7327872330449736
1108	25	0.7907288686880918
1109	1	0.305722056758885
1109	2	0.19992953334482877
1109	3	0.40663627094860644
1109	4	0.2697703174273727
1109	5	0.09733620399369869
1109	6	0.31332073233980395
1109	7	0.36983495428952295
1109	8	0.3001350945517913
1109	9	0.39917921771550624
1109	10	0.09229256593055624
1109	11	0.4603465753579954
1109	12	0.6274302684851918
1109	13	0.5453571312618237
1109	14	0.6297745904606415
1109	15	0.6409669579436681
1109	16	0.5597480466278029
1109	17	0.7627240372674393
1109	18	0.7343076438141694
1109	19	0.7232968897838887
1109	20	0.7564822471978516
1109	21	0.7570290518057305
1109	22	0.6644767979935438
1109	23	0.7645104260244011
1109	24	0.800698979200142
1109	25	0.7594921393900975
1110	1	0.405250159763078
1110	2	0.2051408365004619
1110	3	0.30708880733221733
1110	4	0.3390764330862339
1110	5	0.3909649850495498
1110	6	0.44081717572363743
1110	7	0.35031314706959193
1110	8	0.4376120052290915
1110	9	0.3415768789535534
1110	10	0.5410583627559902
1110	11	0.4534809437431164
1110	12	0.6496726719546118
1110	13	0.555566985956844
1110	14	0.508207800650915
1110	15	0.5272326113571566
1110	16	0.4688681139740538
1110	17	0.4685428334102255
1110	18	0.4905546680163724
1110	19	0.6127227687556811
1110	20	0.6260261398568829
1110	21	0.638222224823636
1110	22	0.5881085046530217
1110	23	0.6484087785080064
1110	24	0.8229746542401486
1110	25	0.7604413830355128
1111	1	0.19509035676013298
1111	2	0.12688935673722668
1111	3	0.18521887794949166
1111	4	0.2539106310431336
1111	5	0.4186576970727742
1111	6	0.40503004452177294
1111	7	0.3623317955144145
1111	8	0.40975103441299865
1111	9	0.4011864842738433
1111	10	0.4813239202667887
1111	11	0.38185611800095387
1111	12	0.612458340533351
1111	13	0.5256589141054775
1111	14	0.39251459727774574
1111	15	0.5161769516912784
1111	16	0.4733996746386353
1111	17	0.5022734365137325
1111	18	0.48369862212657466
1111	19	0.24065972741372121
1111	20	0.5514437873190019
1111	21	0.6786097628175511
1111	22	0.36657252183884026
1111	23	0.39761033483042496
1111	24	0.4858380246723115
1111	25	0.4552153620560162
1112	1	0.20897170217305217
1112	2	-0.1797151998885873
1112	3	0.07118856214150782
1112	4	0.23826237701317865
1112	5	0.17511409308241355
1112	6	0.29407153817050613
1112	7	0.3415687755712895
1112	8	0.22736627028712775
1112	9	0.22465714453863228
1112	10	-0.09871867410281965
1112	11	-0.03052566151129099
1112	12	0.33253209403781625
1112	13	0.1808484110419144
1112	14	0.006856244148210067
1112	15	-0.19629008596980668
1112	16	-0.13524040583025254
1112	17	-0.11269492137614634
1112	18	0.07955373995807946
1112	19	0.05238136360667767
1112	20	-0.005460242892695533
1112	21	0.04333012592240948
1112	22	0.1003594470332061
1112	23	0.29741870644027557
1112	24	0.21511809653440395
1112	25	0.07253048808941998
1113	1	-0.6644657536896581
1113	2	-0.5762538050807251
1113	3	-0.6384153086231628
1113	4	-2.2140946831821253
1113	5	-0.9130945752459496
1113	6	-0.4857300721371871
1113	7	-0.544628420536597
1113	8	-0.4947623902215028
1113	9	-0.4302658082869826
1113	10	-0.4183336332756866
1113	11	-0.6110080902179433
1113	12	-0.2978712630640814
1113	13	-0.4182148359291773
1113	14	-0.6128573135269875
1113	15	-0.6832238788017548
1113	16	-0.9573857908688339
1113	17	-0.8865994534547355
1113	18	-0.7445080161534392
1113	19	-0.6213210393002555
1113	20	-0.3320791034096615
1113	21	-0.44220225891037046
1113	22	-0.19686235296563126
1113	23	-0.15080811226268764
1113	24	-0.2532658161716199
1113	25	-0.36830077037092224
1114	1	-0.9840569170583358
1114	2	-2.104075177363921
1114	3	-1.4776048078188637
1114	4	-1.0485967938121732
1114	5	-0.9271381998941237
1114	6	-0.554626169807527
1114	7	-0.6042491959118763
1114	8	-0.6433181523037419
1114	9	-1.0522831898696552
1114	10	-1.251975780787781
1114	11	-0.9323608158618981
1114	12	-1.0292624540654245
1114	13	-1.1478468229465406
1114	14	-1.2039466806502404
1114	15	-1.3515025595475834
1114	16	-1.7098455496774392
1114	17	-1.7599492899478975
1114	18	-1.4594939570779175
1114	19	-1.792087920660883
1114	20	-1.2504380677099292
1114	21	-1.4926262674867417
1114	22	-1.1114959489068994
1114	23	-0.9715880577438654
1114	24	-1.0979925020022492
1114	25	-1.6697976591748045
1115	1	-1.2942714339205317
1115	2	-1.4863184018544864
1115	3	-1.0268130381191583
1115	4	-0.7650282693268053
1115	5	-1.097653146034574
1115	6	-1.0574099067509124
1115	7	-0.9994569642282514
1115	8	-1.2538888509081403
1115	9	-2.2757571255490916
1115	10	-1.7756553386106546
1115	11	-2.2507581246527337
1115	12	-2.8017712669108477
1115	13	-2.8391420066550097
1115	14	-2.4703962992951816
1115	15	-2.8303461849773086
1115	16	-2.384380847563186
1115	17	-2.218895730815916
1115	18	-2.5876820063661015
1115	19	-3.078657593992837
1115	20	-3.0817615381086867
1115	21	-2.2721247485726934
1115	22	-2.430743954363372
1115	23	-2.3778060643749512
1115	24	-2.989346983835759
1115	25	-2.9904361576310023
1116	1	-2.7050259315994936
1116	2	-2.904397538186032
1116	3	-2.9928909479730486
1116	4	-2.5102303885661037
1116	5	-3.2686997165320797
1116	6	-4.017864559165719
1116	7	-3.5216284001340945
1116	8	-3.905483628395146
1116	9	-4.848604906836751
1116	10	-3.775806509112442
1116	11	-4.633583159058283
1116	12	-6.980219487448643
1116	13	-6.220419682622467
1116	14	-5.450136599782205
1116	15	-6.5950093671163685
1116	16	-6.058883660618044
1116	17	-5.830448865108092
1116	18	-5.836291879511229
1116	19	-6.292347081225348
1116	20	-7.85076302842165
1116	21	-6.9967149125071195
1116	22	-6.310216854198654
1116	23	-7.2278435004661965
1116	24	-6.961821117279381
1116	25	-6.192699436747368
1201	1	0.8059941766427094
1201	2	0.9914999122522268
1201	3	1.0285885712534864
1201	4	0.9583212762414277
1201	5	0.5007106102155419
1201	6	1.0385976667674015
1201	7	1.1692791222223065
1201	8	1.0560515985834797
1201	9	1.1539197390473959
1201	10	1.1339700988199723
1201	11	0.9566764907080341
1201	12	1.3509913929980826
1201	13	1.4712201125245485
1201	14	1.4930172408492197
1201	15	1.4393334907504354
1201	16	1.3192742431681381
1201	17	1.2038793931529028
1201	18	1.4707638927928315
1201	19	1.6293799676815226
1201	20	1.6141720365134162
1201	21	1.2411617739132552
1201	22	1.3698699470940738
1201	23	1.4681431161596166
1201	24	1.3069474904310212
1201	25	1.417803350751747
1202	1	1.0465309459984127
1202	2	1.089967172055097
1202	3	1.0212003211068144
1202	4	0.9778324852060081
1202	5	0.9305381098414856
1202	6	1.1280580077477467
1202	7	1.1807994776014752
1202	8	1.093129070892772
1202	9	1.491166517490882
1202	10	1.4104061647195394
1202	11	1.3939488915999831
1202	12	1.4350404860661996
1202	13	1.403157599841819
1202	14	1.5341521211489848
1202	15	1.5016200258682275
1202	16	1.5395092006398272
1202	17	1.4476099455051212
1202	18	1.478688424973754
1202	19	1.5366648523785997
1202	20	1.3889494962208815
1202	21	1.525556941359575
1202	22	1.4436533852459377
1202	23	1.6258549591751006
1202	24	1.5868053905400217
1202	25	1.6023572403193782
1203	1	0.9115717636910681
1203	2	1.0079504382462368
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2211	16	0.3728646816009231
2211	17	0.22018343129609974
2211	18	0.13367462754554538
2211	19	0.28562944761849707
2211	20	0.3313567594487594
2211	21	0.30971683655664606
2211	22	0.3697645732181343
2211	23	0.16332908686276681
2211	24	0.34888590960047594
2211	25	0.10843063591347146
2212	1	-0.24769704113179003
2212	2	-0.09558156044010957
2212	3	-0.09444756727173953
2212	4	-0.06507376222741357
2212	5	-0.5022728290774481
2212	6	-0.19367874230905358
2212	7	-0.387857053983403
2212	8	-0.20154288991061264
2212	9	-0.13689023104873144
2212	10	-0.4255386117858819
2212	11	-0.25110065460769704
2212	12	-0.011262915538289064
2212	13	-0.051246810155625155
2212	14	0.11275967736028036
2212	15	-0.1912641324393647
2212	16	-0.16537055812742807
2212	17	-0.17764166916955304
2212	18	-0.2939634314383923
2212	19	-0.07475980821312334
2212	20	-0.1888007132801448
2212	21	-0.12926251904316644
2212	22	-0.06775441855094044
2212	23	-0.4822731062247314
2212	24	-0.45212776097264396
2212	25	-0.36511322572226845
2213	1	-0.5542540386287236
2213	2	-0.17593082079691058
2213	3	-0.2630299692042293
2213	4	-0.49341366392745556
2213	5	-0.4146992859246197
2213	6	-0.2773998716446542
2213	7	-0.7145769119925305
2213	8	-0.6595152392501404
2213	9	-0.4386038060721802
2213	10	-0.5059624394451987
2213	11	-0.3523045943389036
2213	12	-0.6382068436885504
2213	13	-0.395945443180473
2213	14	-0.5532372644597388
2213	15	-0.7465307013400135
2213	16	-1.2046532575006026
2213	17	-0.9172075401754824
2213	18	-0.6160002787328409
2213	19	-0.47885405944233383
2213	20	-0.4738754232731779
2213	21	-0.406236550289627
2213	22	-0.5626718743882293
2213	23	-0.7150204418245423
2213	24	-0.4397951576624095
2213	25	-0.4540839852132592
2214	1	-1.5403067085860371
2214	2	-0.9063533197520677
2214	3	-0.9837637976030571
2214	4	-1.2348838655457612
2214	5	-1.4806671546275325
2214	6	-2.1546617033478377
2214	7	-1.4527970840028317
2214	8	-1.4326178062890342
2214	9	-1.1575024272687742
2214	10	-1.5597181053415667
2214	11	-1.5345582875098742
2214	12	-1.2425415541613702
2214	13	-1.6018285302045285
2214	14	-1.1639350053441362
2214	15	-1.287612670992047
2214	16	-1.3443996367591353
2214	17	-1.4269537138908106
2214	18	-1.4158971156186482
2214	19	-1.504046560443129
2214	20	-1.7760031380340138
2214	21	-1.879440322888371
2214	22	-2.295341890430386
2214	23	-2.431371061341551
2214	24	-2.252362754461025
2214	25	-2.4514726077116222
2215	1	-1.992336598863723
2215	2	-1.3810768545362713
2215	3	-1.3624341784812763
2215	4	-1.5412900358598094
2215	5	-1.9133596523747194
2215	6	-1.8672776320526694
2215	7	-2.436339128405667
2215	8	-2.011815644216675
2215	9	-2.25164536050152
2215	10	-2.174849390129104
2215	11	-1.8784441577021198
2215	12	-2.135162214445714
2215	13	-2.352452173108027
2215	14	-2.587272663623267
2215	15	-2.470232919418258
2215	16	-2.415728589767866
2215	17	-2.6108854649052446
2215	18	-2.1993378411785898
2215	19	-2.4255557166241744
2215	20	-2.490507693158875
2215	21	-2.2990329455600573
2215	22	-2.288044426022374
2215	23	-2.788005551700202
2215	24	-2.6385218133764994
2215	25	-2.773402869245025
2216	1	-2.631724425321636
2216	2	-1.9810337095843842
2216	3	-1.990398409377061
2216	4	-2.3151832193815913
2216	5	-3.3699809784639037
2216	6	-3.423269902319433
2216	7	-3.626282597611258
2216	8	-3.6934844327827783
2216	9	-5.860256656601326
2216	10	-3.8381070400259993
2216	11	-3.8283900750201525
2216	12	-4.283218843407003
2216	13	-4.50785059431789
2216	14	-4.442608488294788
2216	15	-4.2483106784985445
2216	16	-3.7094463333111207
2216	17	-4.1701688708840905
2216	18	-4.551311692429092
2216	19	-4.491411759129225
2216	20	-3.886089819756588
2216	21	-4.677107432410843
2216	22	-4.174518786589342
2216	23	-5.3541516712904915
2216	24	-4.711754188330374
2216	25	-4.663483953880029
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	63798.0
3	1.0
4	1048.0
5	9131.0
6	11812.0
7	9552.0
8	7578.0
9	6243.0
10	5331.0
11	4706.0
12	4069.0
13	3678.0
14	3614.0
15	3634.0
16	3798.0
17	4010.0
18	4392.0
19	5446.0
20	7344.0
21	9853.0
22	12168.0
23	14340.0
24	17064.0
25	19216.0
26	21218.0
27	23335.0
28	26086.0
29	31258.0
30	38583.0
31	49196.0
32	62924.0
33	81309.0
34	105231.0
35	139121.0
36	191135.0
37	300553.0
38	974922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.775580227321186	20.288646469619806	14.190890887150484	25.744882415908528
2	12.835953377495086	21.321952344857134	41.596018851135355	24.24607542651243
3	17.260689578822095	26.418346945580446	30.187997060088506	26.132966415508957
4	11.038872331219759	17.776772359991302	38.34609750258615	32.83825780620279
5	11.78940579827296	39.896822161225586	35.49109417888259	12.822677861618864
6	30.284294055469974	38.46498350756059	18.36024937535298	12.890473061616456
7	26.881873101291003	32.73694108007337	22.324224218987993	18.056961599647632
8	24.77205744688519	37.97870573106427	20.5295088148921	16.719728007158448
9	26.644403428849095	14.929007921008708	20.534431938153833	37.89215671198836
10	15.052858526535612	30.174844474288818	33.79698071815172	20.975316281023844
11	33.32640762770569	23.105296056345082	25.178088004487503	18.39020831146172
12	23.290320483650785	25.877679994237234	31.866785201635363	18.965214320476623
13	28.725639802474078	22.88644016723463	26.411931675921853	21.975988354369438
14	20.4135506444928	23.446395926689547	28.260611752055848	27.8794416767618
15	22.407102909333883	32.43709373241438	23.91367281931748	21.242130538934255
16	20.720295297614786	28.889519491250322	29.057453976155323	21.332731234979573
17	20.26672535133934	29.637428873386064	28.70528258690471	21.390563188369892
18	20.113433905773956	27.74509700566818	31.921383415738074	20.22008567281979
19	22.602318233788548	27.00348363184982	29.21269744497061	21.181500689391022
20	23.07745692262214	27.34774948729696	29.74020263904256	19.83459095103834
21	22.113472678964936	26.912678894657986	28.72719989423237	22.246648532144704
22	22.41037615266427	28.49405406640014	28.758210413224155	20.337359367711432
23	21.082954824467198	28.208013626758415	29.729076816106843	20.979954732667544
24	22.251182304891692	27.07922925185038	30.1492469134013	20.52034152985663
25	21.482437056841555	27.812835875832402	29.944300888524033	20.76042617880201
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	194.0
1	194.0
2	605.5
3	1017.0
4	1017.0
5	1017.0
6	2578.5
7	4140.0
8	4140.0
9	4140.0
10	5277.0
11	6414.0
12	6414.0
13	6414.0
14	9072.0
15	11730.0
16	11730.0
17	11730.0
18	21827.5
19	31925.0
20	31925.0
21	31925.0
22	51118.5
23	70312.0
24	70312.0
25	70312.0
26	102805.5
27	135299.0
28	135299.0
29	135299.0
30	170499.0
31	205699.0
32	205699.0
33	205699.0
34	224474.0
35	243249.0
36	243249.0
37	243249.0
38	253422.0
39	263595.0
40	263595.0
41	263595.0
42	272169.5
43	280744.0
44	280744.0
45	280744.0
46	288983.0
47	297222.0
48	297222.0
49	297222.0
50	277466.0
51	257710.0
52	257710.0
53	257710.0
54	221839.0
55	185968.0
56	185968.0
57	185968.0
58	157073.0
59	128178.0
60	128178.0
61	128178.0
62	104371.5
63	80565.0
64	80565.0
65	80565.0
66	61127.5
67	41690.0
68	41690.0
69	41690.0
70	30599.5
71	19509.0
72	19509.0
73	19509.0
74	13645.5
75	7782.0
76	7782.0
77	7782.0
78	5227.0
79	2672.0
80	2672.0
81	2672.0
82	1757.0
83	842.0
84	842.0
85	842.0
86	514.0
87	186.0
88	186.0
89	186.0
90	117.5
91	49.0
92	49.0
93	49.0
94	27.5
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0852990099253436
2	0.04269342824275694
3	0.019238396677291707
4	0.006237105772090005
5	0.019018780276866005
6	0.062019671480218926
7	0.07888621103291303
8	0.058988965154344215
9	0.026266121490914247
10	0.05222478002123251
11	0.006764185133111697
12	5.710026411068315E-4
13	0.005929642811494019
14	0.0010102354419582404
15	0.014670375548437057
16	0.00939958193822015
17	0.005402563450472329
18	0.005666103130983174
19	0.0021961640042570444
20	0.0016690846432353536
21	3.513862406811271E-4
22	6.588492012771132E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2276697.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.326521548347685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4906304589349	39.18999688770049
2	14.824218244885943	15.810479873466383
3	5.280982326801803	8.44849253539919
4	2.364184327719623	5.042949059856254
5	1.2493340243524613	3.3311318885357726
6	0.7495595553434163	2.3982842267874362
7	0.45789661805033055	1.709262370856354
8	0.3132299233997327	1.3362769807810513
9	0.21202578327825838	1.0175937750714008
>10	0.947470358039563	8.624487079915403
>50	0.06044140701172453	2.2118353119853684
>100	0.041763185685093	4.29511337510314
>500	0.004958271898294134	1.851821246363787
>1k	0.0029749631389763406	3.00944088181012
>5k	1.6527572994313005E-4	0.6041708935235499
>10k+	1.6527572994313005E-4	1.118663612844297
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14568	0.6398743442803324	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10817	0.4751181206809689	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8430	0.3702732511177377	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5280	0.23191491884954388	No Hit
GTCCTACAGTGGACATTTCTAAATT	4726	0.2075814216823758	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4454	0.19563428949921752	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4429	0.194536207497089	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4115	0.18074429755035476	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3478	0.15276516813612	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3002	0.13185768681559296	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2737	0.12021801759303061	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2593	0.11389306526077031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.3923280085140884E-5	0.0
10	0.0	0.0	0.0	4.3923280085140884E-5	0.0
11	0.0	0.0	0.0	8.784656017028177E-5	0.0
12	0.0	0.0	0.0	8.784656017028177E-5	0.0
13	0.0	0.0	0.0	8.784656017028177E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1060	0.0	13.816337	4
CCATCGC	110	2.7866918E-9	12.94276	19
TCCAACG	250	0.0	12.909098	18
GGTATCA	2510	0.0	12.200548	1
TCGAGAC	55	0.003043731	12.104368	3
GTCTTAC	165	0.0	12.1041	1
GGACCGG	80	2.8371813E-5	11.887433	6
AGGACCT	1855	0.0	11.637508	5
CCCGTAC	180	0.0	11.602077	16
GGCGAGG	1190	0.0	11.325822	19
GGACCTG	1790	0.0	11.210048	6
CCACGAC	80	3.733542E-4	10.699396	3
GTATCAA	5250	0.0	10.633493	1
TGTAGGA	3175	0.0	10.544007	2
GTAGGAC	2980	0.0	10.499929	3
CGTGCGC	190	9.094947E-12	10.497133	10
GTGCGCC	190	9.094947E-12	10.4934435	11
CCAACGA	210	0.0	10.395296	19
GCGCCAC	185	6.002665E-11	10.263181	13
CTGTAGG	3140	0.0	10.206984	1
>>END_MODULE