FastQCFastQC Report
Thu 26 May 2016
SRR1512913_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512913_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT91660.43278086018855194No Hit
GTCCTAAAGTGTGTATTTCTCATTT76690.3620986708254424No Hit
CTGTAGGACGTGGAATATGGCAAGA73980.34930316426739116No Hit
CTTTAGGACGTGAAATATGGCGAGG66440.31370238218336666No Hit
GTATCAACGCAGAGTACTTTTTTTT57570.2718218865487119No Hit
GTCCTACAGTGTGCATTTCTCATTT53030.25038587187212424No Hit
CTGTAGGACCTGGAATATGGCGAGA33320.15732335000526457No Hit
CTGAAGGACCTGGAATATGGCGAGA32640.15411266939291224No Hit
TATCAACGCAGAGTACTTTTTTTTT32520.15354607869661477No Hit
GGTATCAACGCAGAGTACTTTTTTT32010.15113806823735051No Hit
GTCCTTCAGTGTGCATTTCTCATTT28790.135934551220035No Hit
TTTCTAAATTTTCCACCTTTTTCAG28420.13418756323978448No Hit
ATTTAGAAATGTCCACTGTAGGACG24690.11657603576320476No Hit
GAATATGGCAAGAAAACTGAAAATC24080.11369586639035927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT400.00528647914.247349
TAGGACC14750.012.9433484
CACCTTT9250.011.81028114
CCACCTT9250.011.81000213
CGCCCTA2900.011.79229616
ACGAAAT2750.011.7446512
GTCCTAG3950.011.5694931
GGACCTG27050.011.3419596
ACCTTTT9550.011.33980715
GGTATCA19150.011.2359511
TAGAAAT9050.011.2299494
AGGACCT28000.011.2279975
CGAGCAA600.00588501611.08126510
AGGACGA1658.0035534E-1110.9370955
TCCCGCC3100.010.72507213
TGTAGGA41700.010.6826592
GTCCTAC45150.010.6699651
GGCGAGG18050.010.63034919
TGTCCAC9150.010.58818810
CTGTAGG41650.010.5836681
Produced by FastQC (version 0.11.5)