##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512913_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2117931
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.05391063259379	31.0	30.0	34.0	23.0	34.0
2	29.1398444991834	31.0	30.0	34.0	22.0	34.0
3	29.302034391110947	31.0	30.0	34.0	23.0	34.0
4	32.720687784446234	35.0	33.0	37.0	25.0	37.0
5	32.92078117747934	35.0	35.0	37.0	26.0	37.0
6	32.799331045251236	36.0	33.0	37.0	25.0	37.0
7	32.77444779834659	37.0	33.0	37.0	25.0	37.0
8	32.69272983869635	37.0	33.0	37.0	25.0	37.0
9	34.00319179425581	39.0	34.0	39.0	24.0	39.0
10	33.86287796911231	38.0	34.0	39.0	23.0	39.0
11	33.920122043635985	39.0	34.0	39.0	23.0	39.0
12	33.823039560778895	38.0	34.0	39.0	22.0	39.0
13	33.798577951784075	38.0	34.0	39.0	22.0	39.0
14	34.65331684554407	39.0	34.0	41.0	19.0	41.0
15	34.8449038235901	39.0	34.0	41.0	22.0	41.0
16	34.7939791239658	39.0	34.0	41.0	22.0	41.0
17	34.565175636033466	39.0	34.0	41.0	18.0	41.0
18	34.56784050094172	39.0	34.0	41.0	18.0	41.0
19	34.67498516240614	39.0	34.0	41.0	19.0	41.0
20	34.64946166801468	39.0	34.0	41.0	18.0	41.0
21	34.54465230453683	39.0	34.0	41.0	18.0	41.0
22	34.5626146460862	39.0	34.0	41.0	18.0	41.0
23	34.234118108663594	39.0	34.0	41.0	16.0	41.0
24	34.301114153388376	39.0	34.0	41.0	16.0	41.0
25	34.015667177070455	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7000207593594325
1101	2	0.4207675249432299
1101	3	0.7255386150398699
1101	4	0.8781565083046416
1101	5	0.7528739888998217
1101	6	0.716903144260506
1101	7	0.4315079633310006
1101	8	0.7062242153277651
1101	9	0.8593770036801942
1101	10	0.7143138616597611
1101	11	0.8104064428019271
1101	12	0.7224011041000438
1101	13	1.0647997617810248
1101	14	1.2517286223454462
1101	15	1.0570751624558596
1101	16	0.9749374638500683
1101	17	0.17256153839301192
1101	18	0.7548814439357514
1101	19	0.3111189444397482
1101	20	-0.25738241713228405
1101	21	0.8207205054889144
1101	22	0.9220388342131898
1101	23	0.46657330897924965
1101	24	1.0647822773575228
1101	25	0.639770719127192
1102	1	1.0272925451562323
1102	2	0.9559254970650919
1102	3	0.8562182964921021
1102	4	1.0987671713312608
1102	5	0.9844146264196354
1102	6	0.8979879917191411
1102	7	0.83956735142214
1102	8	0.8685109530596762
1102	9	1.2785215169390725
1102	10	1.1800667368167908
1102	11	0.9320526714930608
1102	12	1.0449759418812903
1102	13	0.9865259089799636
1102	14	1.1704633218275546
1102	15	1.226253521511353
1102	16	1.124420558501427
1102	17	1.1722229840000224
1102	18	1.1471208631225949
1102	19	1.0629197143882294
1102	20	1.306869054629523
1102	21	1.48259540448187
1102	22	1.2400264576442765
1102	23	1.198989308726226
1102	24	0.5450163321302384
1102	25	1.053277544792607
1103	1	0.7005191306849916
1103	2	0.7490797480914679
1103	3	0.8887666722006955
1103	4	0.9587743666359145
1103	5	0.8919352343854783
1103	6	0.5934093246755054
1103	7	0.5495910577819103
1103	8	0.7652354252481786
1103	9	0.7765710433728401
1103	10	0.9954265805844429
1103	11	0.6990528876513835
1103	12	0.8484005179604139
1103	13	0.9746468312355177
1103	14	1.0004624691615192
1103	15	0.9642842219765058
1103	16	0.9877432409887845
1103	17	0.6543595369704036
1103	18	1.0607745894601806
1103	19	1.0130925101530224
1103	20	1.2626718771037062
1103	21	0.9481203961982914
1103	22	0.9699584201299629
1103	23	0.4502197843568041
1103	24	0.4656578997492744
1103	25	0.8465074325323911
1104	1	0.9897254589209474
1104	2	1.003064070624525
1104	3	0.9539401812449633
1104	4	1.1363679194978218
1104	5	0.9390840602268753
1104	6	0.9414942296088427
1104	7	1.0919232461316994
1104	8	0.7939637779031088
1104	9	1.200653680358009
1104	10	1.2343136345638115
1104	11	0.9001422734337652
1104	12	1.2545680158410946
1104	13	1.1692139393814145
1104	14	1.4513795569399193
1104	15	1.1922779686048557
1104	16	1.173940134119988
1104	17	1.4792984344833613
1104	18	1.2654987475119412
1104	19	0.8242420626185876
1104	20	0.9875683882067392
1104	21	1.1103227280013144
1104	22	0.8759153621431537
1104	23	1.2816597663012956
1104	24	1.2600372966512126
1104	25	0.6771833581524973
1105	1	0.5065024749922209
1105	2	0.5550958692142984
1105	3	0.7088361699150099
1105	4	0.7654331668668419
1105	5	0.6191047336209294
1105	6	0.7238273685116852
1105	7	0.7368934461795718
1105	8	0.6814874923606098
1105	9	0.4951183386879663
1105	10	0.49644266186804487
1105	11	0.6271664497198017
1105	12	0.7467235763958655
1105	13	0.7742927510833439
1105	14	0.8767274550475648
1105	15	0.8847604238353313
1105	16	0.7049345540099097
1105	17	1.122838290712572
1105	18	0.9040800425806381
1105	19	0.5936799250147899
1105	20	0.8046791691302815
1105	21	0.7585660956990026
1105	22	0.7381826012347261
1105	23	0.9640286929866306
1105	24	1.0786391304046177
1105	25	1.297226222665877
1106	1	0.8761492232858892
1106	2	0.8023073286913132
1106	3	0.7624402794509457
1106	4	0.8415989629641629
1106	5	0.7065959468564103
1106	6	0.895246995126314
1106	7	0.954227529024223
1106	8	0.820136876135031
1106	9	1.0657956528529198
1106	10	0.857275489878603
1106	11	0.5679836133339293
1106	12	0.7724239939911897
1106	13	0.8906574005307561
1106	14	1.1897166702770363
1106	15	0.8374235358092506
1106	16	0.9193820795619345
1106	17	1.1958610583989326
1106	18	0.9957193218501672
1106	19	1.018373421455827
1106	20	1.0658725562167888
1106	21	0.9629827203671866
1106	22	0.9426593891813013
1106	23	1.1544222449461259
1106	24	0.8305481715738523
1106	25	1.083686632302829
1107	1	0.756295246257487
1107	2	0.7760505581799748
1107	3	0.7695837932974641
1107	4	0.8727903702604607
1107	5	0.6623320775336126
1107	6	0.7962024317329011
1107	7	0.5118349703971319
1107	8	0.6346330096500168
1107	9	0.7205178537595671
1107	10	0.8682252219538498
1107	11	0.8636330756746133
1107	12	0.8616613604088741
1107	13	0.5734588538831815
1107	14	0.9360432923402513
1107	15	0.7405639599426053
1107	16	0.7338935964894802
1107	17	1.0370968205981583
1107	18	0.9172208442650671
1107	19	0.7417622895117546
1107	20	0.8483610566634567
1107	21	0.7679907269809689
1107	22	0.7221486671237329
1107	23	1.1467833852153362
1107	24	0.9311413921582385
1107	25	1.1138442742754364
1108	1	0.7264953142707462
1108	2	0.7367894279311216
1108	3	0.786823683680165
1108	4	0.9091800602809457
1108	5	0.776602747810351
1108	6	0.8446789260560408
1108	7	0.8097776125478688
1108	8	0.839640550814913
1108	9	0.9301531521223154
1108	10	0.9831737750279927
1108	11	0.7144172616398237
1108	12	0.8399608039288822
1108	13	0.8743893202758954
1108	14	0.9860005893583121
1108	15	0.9215422259852062
1108	16	0.74556557182472
1108	17	1.0497331824252356
1108	18	1.1369946179712755
1108	19	1.0213636764284644
1108	20	1.1374065952618437
1108	21	1.1360899986506752
1108	22	1.1596025137235415
1108	23	1.4805581124916856
1108	24	1.2114024038598075
1108	25	1.4252621084598047
1109	1	0.5473756171650734
1109	2	0.5413775850334908
1109	3	0.6544936682935258
1109	4	0.6985587289141861
1109	5	0.5530199636745863
1109	6	0.7435831322135584
1109	7	0.6115085115930654
1109	8	0.5649184662443432
1109	9	0.6205995206550199
1109	10	0.6854193395016424
1109	11	0.5660664151146975
1109	12	0.5446801961632488
1109	13	0.7367656776620635
1109	14	0.7963183500629469
1109	15	0.6755126415024293
1109	16	0.291716246404512
1109	17	0.7763354151133228
1109	18	0.7672273424724452
1109	19	0.714457378832968
1109	20	0.6573526444860747
1109	21	0.6574706598728426
1109	22	0.589161243721243
1109	23	0.9020905590449075
1109	24	0.5538425969276233
1109	25	0.21080205193374724
1110	1	0.7200901922268557
1110	2	0.8429344943184773
1110	3	0.7016845936952585
1110	4	0.682799429164632
1110	5	0.4745667148271764
1110	6	0.6870790369583517
1110	7	0.6369355867322781
1110	8	0.7024685084666586
1110	9	0.8985934856507569
1110	10	0.7131392207423346
1110	11	0.6967006170509364
1110	12	0.7705182420854371
1110	13	0.8081588180321759
1110	14	0.7509133564737169
1110	15	0.645596218981936
1110	16	0.8218422320220853
1110	17	0.980676468214341
1110	18	0.4055268566577013
1110	19	0.7374876405700519
1110	20	0.8837393740836106
1110	21	0.975724483108948
1110	22	0.8723434438653541
1110	23	1.1103379508618332
1110	24	0.890374606400286
1110	25	1.1872272358434302
1111	1	0.41384083686245887
1111	2	0.4068828615854301
1111	3	0.5058989633963549
1111	4	0.48406343064986856
1111	5	0.44438178176613974
1111	6	0.413358509698007
1111	7	0.21947833896529545
1111	8	0.47211751727495965
1111	9	0.4726167337890459
1111	10	0.3864446026760362
1111	11	0.20756762614803392
1111	12	-0.08715704722701645
1111	13	0.25906698066599887
1111	14	0.33844558825374094
1111	15	0.31138927548295214
1111	16	0.2981743070267271
1111	17	-0.008870120889774569
1111	18	0.3363058858880592
1111	19	0.32566760220133517
1111	20	0.5353066121552743
1111	21	0.5045551657006939
1111	22	0.23568791650185972
1111	23	0.4713472954994913
1111	24	0.5732128595925232
1111	25	0.6171558617897546
1112	1	0.4486577147631401
1112	2	0.4831770379093001
1112	3	0.41097583278111927
1112	4	0.6081398418134043
1112	5	0.48939634330303505
1112	6	0.29229560710115976
1112	7	0.17965430615767986
1112	8	0.49581566126565235
1112	9	0.37449834205170873
1112	10	0.3274302305713874
1112	11	0.3492346128015953
1112	12	0.3827885891330496
1112	13	0.3576411190692923
1112	14	0.38013800319908597
1112	15	0.17503296197089213
1112	16	0.3682274589829575
1112	17	0.6722689129887343
1112	18	0.459092963196035
1112	19	0.37969563987957855
1112	20	0.29137795813960565
1112	21	0.47842844918433514
1112	22	0.30556076650992736
1112	23	0.49650031323189125
1112	24	0.5236201815752821
1112	25	0.47005950818973474
1113	1	-0.20349663760997316
1113	2	-0.0520429240964404
1113	3	0.048967519103182866
1113	4	0.06827072869842965
1113	5	-0.21103024030102802
1113	6	-0.4037298936943259
1113	7	-0.42926123727704635
1113	8	-0.3462275871044369
1113	9	-0.4930786443026207
1113	10	-0.37858936317374514
1113	11	-0.3603816236250523
1113	12	-0.5844497066325118
1113	13	-0.42744542147831766
1113	14	-0.06564768664982523
1113	15	-0.4380375951206332
1113	16	-0.44052300690565005
1113	17	-0.227975556843937
1113	18	-0.6467750081441679
1113	19	-0.8373411764462659
1113	20	-0.7057856841914472
1113	21	-0.45144079952508065
1113	22	-0.9302497962278835
1113	23	-4.525697888299007
1113	24	-1.9855450468631162
1113	25	-1.0935674902637942
1114	1	-0.6499280984053328
1114	2	-0.8727902737979996
1114	3	-0.6891214943685426
1114	4	-0.5368466020660065
1114	5	-0.728640810482247
1114	6	-1.3207004744827344
1114	7	-1.0693010067355395
1114	8	-1.134577884944644
1114	9	-1.3340308677666783
1114	10	-0.9869255555533556
1114	11	-1.0715840223773654
1114	12	-1.8292442448303667
1114	13	-1.1921895792856816
1114	14	-2.877247461621657
1114	15	-1.65332068939275
1114	16	-1.595675769867995
1114	17	-1.4894042197691206
1114	18	-1.5422537698000838
1114	19	-1.589632471062373
1114	20	-1.1803936078197026
1114	21	-1.1110691247582025
1114	22	-1.1707977143428039
1114	23	-5.5207722135141495
1114	24	-2.7560570496137906
1114	25	-2.6426326570423235
1115	1	-1.5576169972049918
1115	2	-1.4289861845991751
1115	3	-1.0902006264804314
1115	4	-1.103216055602747
1115	5	-0.9407529251898872
1115	6	-1.2670229629076388
1115	7	-1.7056324478554252
1115	8	-2.1744856896297478
1115	9	-3.4122542268989235
1115	10	-3.882609168538579
1115	11	-2.839128405162352
1115	12	-3.2431042801225587
1115	13	-2.413846143723166
1115	14	-3.20807193458419
1115	15	-2.6686110815339887
1115	16	-2.7143565414686677
1115	17	-2.6750579385790694
1115	18	-5.254099283717306
1115	19	-3.208307915361665
1115	20	-3.410992684075076
1115	21	-3.10607456756302
1115	22	-2.5295966483167938
1115	23	-3.2216600813631295
1115	24	-2.8171655337419708
1115	25	-3.021092256061536
1116	1	-1.7167706143094073
1116	2	-1.4968215069486774
1116	3	-1.9360218513134164
1116	4	-1.8146644828530363
1116	5	-1.8403095009111041
1116	6	-4.154324150793716
1116	7	-2.6983404455518674
1116	8	-3.133825268750037
1116	9	-4.0492396987442945
1116	10	-3.4011313126458447
1116	11	-3.0490593700883615
1116	12	-2.936137762491459
1116	13	-4.216582076637724
1116	14	-7.389287213747135
1116	15	-5.161510974027898
1116	16	-3.9198030395420496
1116	17	-4.914642377915865
1116	18	-3.6885554471102004
1116	19	-3.450309349458177
1116	20	-4.355069568832839
1116	21	-4.989302798601901
1116	22	-4.765380731891277
1116	23	-3.795877030910958
1116	24	-4.595285946704482
1116	25	-4.472475975076819
1201	1	0.4757028957917804
1201	2	0.5621332421008454
1201	3	0.8957504823003966
1201	4	0.8364019415555362
1201	5	0.3731134184080105
1201	6	0.8841024344936983
1201	7	0.8320168138100925
1201	8	0.9079338338125353
1201	9	1.3180356759973861
1201	10	1.170784391271468
1201	11	1.3521856326042112
1201	12	1.2160250958687442
1201	13	1.2773973440113124
1201	14	1.4832498481958751
1201	15	1.3845856141146697
1201	16	1.3161837835308745
1201	17	1.481668934223869
1201	18	1.3372028314224096
1201	19	1.5926830708508035
1201	20	1.554726947374938
1201	21	1.6680093029535001
1201	22	1.45481756814835
1201	23	1.8313515788720451
1201	24	1.6654269147290464
1201	25	1.7293185347606723
1202	1	0.8582253903620547
1202	2	0.7811279675119529
1202	3	0.8149013769120437
1202	4	1.0588716902368915
1202	5	0.8715713768318452
1202	6	1.080971810851132
1202	7	1.0577164725131638
1202	8	1.0810582520564012
1202	9	1.3306564227136946
1202	10	1.1833606659637823
1202	11	1.0515257918761094
1202	12	1.223140619707813
1202	13	1.2096623145356702
1202	14	1.5864611420215056
1202	15	1.3524726758583867
1202	16	1.0702338404136924
1202	17	1.3928676104054816
1202	18	1.5026480687789103
1202	19	1.496858269940681
1202	20	1.2495785349227688
1202	21	1.411144063528532
1202	22	1.3806651221870325
1202	23	1.5409906315145108
1202	24	1.516481832507516
1202	25	1.74515264376884
1203	1	0.9591427432303306
1203	2	1.030178117604212
1203	3	0.9212108515432362
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2211	18	-0.20391396410599327
2211	19	-0.08764641819629304
2211	20	-0.1718537248951364
2211	21	-0.06768050502697776
2211	22	1.759252852764348E-4
2211	23	0.22498302178492935
2211	24	-0.16672920160038274
2211	25	0.24411989475402862
2212	1	0.2558808927544014
2212	2	0.480936579952143
2212	3	0.07089818711937923
2212	4	0.3832325924925257
2212	5	0.1691175149042934
2212	6	0.07116319841093599
2212	7	0.20137334195950984
2212	8	0.36079851179666633
2212	9	0.4179952242630236
2212	10	0.15895419524152032
2212	11	-0.15149995430753194
2212	12	0.41240468897188265
2212	13	0.5117744129635611
2212	14	0.6403982575375693
2212	15	0.19505640791580703
2212	16	0.20155532594570502
2212	17	0.48144815267023233
2212	18	0.573550324418008
2212	19	0.4220097850921931
2212	20	0.4754614239635515
2212	21	0.564215378178794
2212	22	0.5409430898334193
2212	23	0.4321776900699916
2212	24	0.3827907867112046
2212	25	0.5547174649126063
2213	1	-0.10521661969924878
2213	2	0.024188692695446434
2213	3	-0.26901364265160055
2213	4	-0.11082289700230064
2213	5	-0.03022013978580418
2213	6	0.006350759087652591
2213	7	-0.026170873887032542
2213	8	0.03116353836368546
2213	9	-0.21450462226894018
2213	10	0.08355950955542824
2213	11	-0.06987032838302554
2213	12	-0.12216181498077106
2213	13	-0.06387068708622934
2213	14	-0.1308973537313065
2213	15	-0.07236994447863765
2213	16	0.036457095034286624
2213	17	-0.023307628527540203
2213	18	-0.05860684504025926
2213	19	-0.09485746619295554
2213	20	0.09237773954840378
2213	21	-0.09057066464220043
2213	22	-0.3457277451009091
2213	23	-0.1106528061887957
2213	24	0.08612611323435004
2213	25	0.3221665078404641
2214	1	-2.6292644332471724
2214	2	-1.5337420008281661
2214	3	-1.5883686366564795
2214	4	-3.4127825386188313
2214	5	-1.7507250953526885
2214	6	-1.450352250659499
2214	7	-1.370770637391857
2214	8	-1.173910088061188
2214	9	-0.9049869513252062
2214	10	-1.017351681390359
2214	11	-0.9909233261700265
2214	12	-0.9115043373251979
2214	13	-1.2480853109731456
2214	14	-1.2127756286704923
2214	15	-1.6063412092614655
2214	16	-1.394195150992907
2214	17	-3.5979600693774216
2214	18	-3.2669912347036743
2214	19	-2.165564841773481
2214	20	-1.9471390136977647
2214	21	-1.9601098939567692
2214	22	-3.0163577181567014
2214	23	-1.6789042124474989
2214	24	-2.956703377804562
2214	25	-2.816482363015421
2215	1	-1.8238743092039833
2215	2	-3.4862115228630337
2215	3	-2.1534836978255427
2215	4	-3.585045078428962
2215	5	-2.2627971554926276
2215	6	-2.061811097794692
2215	7	-2.3624927185405795
2215	8	-1.588199564790152
2215	9	-4.013148332302812
2215	10	-2.3545236855068623
2215	11	-1.9700416574024793
2215	12	-1.8675533456495508
2215	13	-2.0075803693410386
2215	14	-1.6864998015636772
2215	15	-1.889460115952005
2215	16	-1.8957833696304434
2215	17	-2.267854172911157
2215	18	-1.8708770230203555
2215	19	-2.0056891133166204
2215	20	-1.8254513230201823
2215	21	-2.352562030459083
2215	22	-2.3022427818224642
2215	23	-2.3348392709371772
2215	24	-2.1604737700294834
2215	25	-3.099396890743975
2216	1	-3.2338196345563155
2216	2	-3.3529088085664114
2216	3	-4.641265776665321
2216	4	-5.123787414993103
2216	5	-4.761685769298651
2216	6	-3.7495597810604266
2216	7	-5.5782387317029105
2216	8	-3.333235154599951
2216	9	-4.231959719807932
2216	10	-3.036625585969542
2216	11	-3.31639309453049
2216	12	-3.6834527239365684
2216	13	-3.2817285315432656
2216	14	-4.409291278211068
2216	15	-3.7400976397743086
2216	16	-3.9708516840177666
2216	17	-3.5960273887352656
2216	18	-3.3832183458398646
2216	19	-3.9339952558655895
2216	20	-4.491588905072646
2216	21	-4.228720174313022
2216	22	-3.8237262394444045
2216	23	-3.8003051237699026
2216	24	-4.289014529983177
2216	25	-4.233526396157835
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97894.0
3	1.0
4	910.0
5	5438.0
6	6627.0
7	6001.0
8	5283.0
9	5033.0
10	4382.0
11	4105.0
12	3870.0
13	3728.0
14	3852.0
15	4033.0
16	3966.0
17	4310.0
18	4996.0
19	6031.0
20	7942.0
21	10106.0
22	12803.0
23	15181.0
24	17779.0
25	20215.0
26	23056.0
27	25472.0
28	28463.0
29	33573.0
30	41062.0
31	50559.0
32	63823.0
33	81183.0
34	105116.0
35	137701.0
36	187202.0
37	286089.0
38	800146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.96911270074376	18.703207162821545	12.490170069638543	24.837510066796153
2	14.107395740416932	19.865714006438058	43.60757966472416	22.419310588420842
3	19.382939149068186	25.72566437287894	28.80161907761496	26.089777400437917
4	11.993413621544843	16.45560474929452	38.33798302312316	33.21299860603748
5	12.407652347359868	39.54961025689943	35.225485284110846	12.817252111629864
6	31.255339497539108	36.669022975905165	18.816389327809237	13.259248198746493
7	28.400039502514034	31.50320768919271	21.922099719276993	18.174653089016267
8	24.914973220581867	38.28832916470524	20.443906902529722	16.35279071218317
9	27.9655915116656	14.577487617083651	20.105074042326372	37.35184682892437
10	16.239945937260757	28.65759950095933	33.99393211912706	21.108522442652855
11	34.312558005901245	21.749543041087552	25.677641241416072	18.26025771159513
12	25.105402376911623	24.297297795446845	31.642783653025912	18.95451617461562
13	29.90578996885498	22.509047933848368	25.521481843411088	22.06368025388557
14	20.59183229843016	23.517725670324268	27.26020017579177	28.630241855453797
15	22.528718794448565	33.45939202631119	22.794665935800325	21.21722324343992
16	20.989682790584702	27.826411167706986	29.89013658146097	21.29376946024734
17	20.958128829644192	29.62873866614364	28.387230935372504	21.025901568839664
18	20.804430545164443	26.768255132553854	32.16488637937252	20.262427942909188
19	23.919759431414274	26.295199356139708	27.60135104799793	22.18369016444808
20	24.4191327385366	27.012448387701987	28.62443921764334	19.943979656118074
21	22.79564364370419	26.525471302257188	26.92579942767065	23.753085626367977
22	23.608865488729087	29.386037256982434	26.896960788715617	20.108136465572855
23	21.463114252457576	28.83889325507902	28.678213257328544	21.019779235134855
24	23.58935050721097	26.587438483007748	29.368049064261214	20.45516194552007
25	22.171841566041874	27.922529620360358	28.93202250164453	20.97360631195324
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	296.0
1	296.0
2	642.5
3	989.0
4	989.0
5	989.0
6	1715.5
7	2442.0
8	2442.0
9	2442.0
10	2835.5
11	3229.0
12	3229.0
13	3229.0
14	4836.5
15	6444.0
16	6444.0
17	6444.0
18	12988.5
19	19533.0
20	19533.0
21	19533.0
22	34724.0
23	49915.0
24	49915.0
25	49915.0
26	78207.5
27	106500.0
28	106500.0
29	106500.0
30	142467.0
31	178434.0
32	178434.0
33	178434.0
34	199363.5
35	220293.0
36	220293.0
37	220293.0
38	232434.5
39	244576.0
40	244576.0
41	244576.0
42	255000.0
43	265424.0
44	265424.0
45	265424.0
46	278424.0
47	291424.0
48	291424.0
49	291424.0
50	273415.0
51	255406.0
52	255406.0
53	255406.0
54	219296.5
55	183187.0
56	183187.0
57	183187.0
58	155413.5
59	127640.0
60	127640.0
61	127640.0
62	105197.0
63	82754.0
64	82754.0
65	82754.0
66	63760.5
67	44767.0
68	44767.0
69	44767.0
70	32814.0
71	20861.0
72	20861.0
73	20861.0
74	14911.0
75	8961.0
76	8961.0
77	8961.0
78	6121.5
79	3282.0
80	3282.0
81	3282.0
82	2201.0
83	1120.0
84	1120.0
85	1120.0
86	727.0
87	334.0
88	334.0
89	334.0
90	213.5
91	93.0
92	93.0
93	93.0
94	54.5
95	16.0
96	16.0
97	16.0
98	13.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3319749321389601
2	0.010009768967921997
3	0.008593292227178317
4	0.010718007338293834
5	0.011992836404963146
6	0.0768202552396655
7	0.07601758508657742
8	0.10042820091872681
9	0.08069195833103156
10	0.08834093273104743
11	0.08343048002980268
12	0.09405405558538026
13	0.08036144709152471
14	0.08456366142239762
15	0.08125854902732903
16	0.08300553700757957
17	0.09613155480513766
18	0.0979257586767463
19	0.09178769280019038
20	0.0903712160594467
21	0.0787088908939904
22	0.09849234937304378
23	0.09084337497302793
24	0.08730218312116872
25	0.08588570638042504
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2117931.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.7716595136025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75300123482005	42.80642251515496
2	13.489779685264658	15.046947990401849
3	4.475438978204274	7.488079765989414
4	1.9703829550945973	4.395661091317671
5	1.0321844718245943	2.878332045891445
6	0.5858527022893533	1.9604386462323449
7	0.36262569454175986	1.415696573680666
8	0.26460506782390875	1.1805970998598987
9	0.17243039621173914	0.8655056412585019
>10	0.7856541175704876	7.672076677512985
>50	0.056172803073972266	2.166180966686668
>100	0.04260591727746885	4.8195103453849
>500	0.005015528295310344	1.8461332091169864
>1k	0.003740393982943278	3.4701463307968554
>5k	5.100537249468106E-4	1.9882711007149283
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	9166	0.43278086018855194	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7669	0.3620986708254424	No Hit
CTGTAGGACGTGGAATATGGCAAGA	7398	0.34930316426739116	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6644	0.31370238218336666	No Hit
GTATCAACGCAGAGTACTTTTTTTT	5757	0.2718218865487119	No Hit
GTCCTACAGTGTGCATTTCTCATTT	5303	0.25038587187212424	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3332	0.15732335000526457	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3264	0.15411266939291224	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3252	0.15354607869661477	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3201	0.15113806823735051	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2879	0.135934551220035	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2842	0.13418756323978448	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2469	0.11657603576320476	No Hit
GAATATGGCAAGAAAACTGAAAATC	2408	0.11369586639035927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.721589135812262E-5	0.0	0.0	0.0	0.0
9	4.721589135812262E-5	0.0	0.0	0.0	0.0
10	4.721589135812262E-5	0.0	0.0	0.0	0.0
11	4.721589135812262E-5	0.0	0.0	0.0	0.0
12	4.721589135812262E-5	0.0	0.0	4.721589135812262E-5	0.0
13	4.721589135812262E-5	0.0	0.0	4.721589135812262E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGGGGT	40	0.005286479	14.24734	9
TAGGACC	1475	0.0	12.943348	4
CACCTTT	925	0.0	11.810281	14
CCACCTT	925	0.0	11.810002	13
CGCCCTA	290	0.0	11.792296	16
ACGAAAT	275	0.0	11.74465	12
GTCCTAG	395	0.0	11.569493	1
GGACCTG	2705	0.0	11.341959	6
ACCTTTT	955	0.0	11.339807	15
GGTATCA	1915	0.0	11.235951	1
TAGAAAT	905	0.0	11.229949	4
AGGACCT	2800	0.0	11.227997	5
CGAGCAA	60	0.005885016	11.081265	10
AGGACGA	165	8.0035534E-11	10.937095	5
TCCCGCC	310	0.0	10.725072	13
TGTAGGA	4170	0.0	10.682659	2
GTCCTAC	4515	0.0	10.669965	1
GGCGAGG	1805	0.0	10.630349	19
TGTCCAC	915	0.0	10.588188	10
CTGTAGG	4165	0.0	10.583668	1
>>END_MODULE