FastQCFastQC Report
Thu 26 May 2016
SRR1512913_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512913_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2117931
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT85610.4042152459168878No Hit
GTATCAACGCAGAGTACTTTTTTTT76180.35969066036617814No Hit
CTGTAGGACGTGGAATATGGCAAGA73500.3470368014822013No Hit
GTCCTAAAGTGTGTATTTCTCATTT72010.340001633669841No Hit
CTTTAGGACGTGAAATATGGCGAGG71510.3376408391019348No Hit
TATCAACGCAGAGTACTTTTTTTTT54580.2577043350326333No Hit
GTCCTACAGTGTGCATTTCTCATTT49080.23173559478566583No Hit
GGTATCAACGCAGAGTACTTTTTTT44740.21124389793624063No Hit
CTGTAGGACCTGGAATATGGCGAGA33670.15897590620279886No Hit
CTGAAGGACCTGGAATATGGCGAGA33410.15774829302748766No Hit
TTTCTAAATTTTCCACCTTTTTCAG28760.13579290354596066No Hit
ATTTAGAAATGTCCACTGTAGGACG25930.12243080629161195No Hit
GTCCTTCAGTGTGCATTTCTCATTT25500.12040052296321269No Hit
ACGCAGAGTACTTTTTTTTTTTTTT24700.11662325165456286No Hit
GAATATGGCAAGAAAACTGAAAATC22100.104347119901451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAGG1051.3878889E-913.56046919
TATTCCG500.001488470813.3134595
TAGGACC13700.013.11910254
TGTCGAG1550.012.86087618
TCCAACG2200.012.51290818
GGTATCA19050.012.3320931
CACCTTT8350.012.2783714
CGGCAAA550.003086612612.08171517
GGCGAGG18200.011.99579819
CCACCTT8450.011.90837913
CCAACGA1302.6502676E-911.68286519
ACCTTTT8950.011.66737415
TAGAAAT11400.011.4282214
TATGTCG1751.8189894E-1211.39133116
GTCCTAA21750.011.2822371
AGGACCT25700.011.2487275
CCTAACG600.005826606411.0961263
AATGTCC10800.011.0851118
CTACACT4500.010.9888894
TGGCGAA1658.185452E-1110.93081618
Produced by FastQC (version 0.11.5)