FastQCFastQC Report
Thu 26 May 2016
SRR1512912_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512912_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050861
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT109220.532556813943022No Hit
TATCAACGCAGAGTACTTTTTTTTT63020.30728557420517527No Hit
GGTATCAACGCAGAGTACTTTTTTT62850.3064566540589538No Hit
GTACTGGTTCACTATCGGTCAGTCA33970.16563774921849897No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30110.14681638589841048No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23690.11551246037639802No Hit
GTCCTACAGTGGACATTTCTAAATT22510.10975877936144868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGGT250.006036569418.9979024
CCGTATG707.279041E-613.5689369
CGACAGT500.001501080713.2985326
TACCGTA655.4597942E-513.1523947
ACCGTAT802.002771E-613.0610578
GTCTTAT1650.012.6996681
TAGGACC5250.012.1224714
GTAATAC1603.6379788E-1211.8736883
GGTATCA22600.011.5898521
TTAGACT1001.9329418E-611.3987414
TAGGACT2200.011.2260324
CCGATTT600.005883713711.08156813
TAATACA2250.010.9765654
CTAATAC1408.36917E-910.8559443
CAACGTC700.001493736510.85541415
CCCCGTA2500.010.63830615
GTAGGAC13500.010.6247533
TGGCGAG12150.010.55387518
TAGGCCC1002.4023226E-510.4488464
CTAGGAC2001.8189894E-1210.4488463
Produced by FastQC (version 0.11.5)